Abstract:
AIM:To characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. METHODOLOGY:Apical lesion samples were collected from 12 patients (23-80 years old) who visited the Kyushu University Hospital for apicectomy with persistent periapical lesions associated with root filled teeth. DNA was directly extracted from each sample and the microbial composition was comprehensively analysed using clone library analysis of the 16S rRNA gene. Enterococcus faecalis, Candida albicans and specific fimA genotypes of Porphyromonas gingivalis were confirmed using polymerase chain reaction (PCR) analysis with specific primers. RESULTS:Bacteria were detected in all samples, and the dominant findings were P. gingivalis (19.9%), Fusobacterium nucleatum (11.2%) and Propionibacterium acnes (9%). Bacterial diversity was greater in symptomatic lesions than in asymptomatic ones. In addition, the following bacteria or bacterial combinations were characteristic to symptomatic lesions: Prevotella spp., Treponema spp., Peptostreptococcaceae sp. HOT-113, Olsenella uli, Slackia exigua, Selemonas infelix, P. gingivalis with type IV fimA, and a combination of P. gingivalis, F. nucleatum, and Peptostreptococcaceae sp. HOT-113 and predominance of Streptococcus spp. On the other hand, neither Enterococcus faecalis nor C. albicans were detected in any of the samples. CONCLUSION:Whilst a diverse bacterial species were observed in the persistent apical lesions, some characteristic patterns of bacterial community were found in the symptomatic lesions. The diverse variation of community indicates that bacterial combinations as a community may cause persistent inflammation in periapical tissues rather than specific bacterial species.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Zakaria MN,Takeshita T,Shibata Y,Maeda H,Wada N,Akamine A,Yamashita Ydoi
10.1111/iej.12361subject
Has Abstractpub_date
2015-08-01 00:00:00pages
717-28issue
8eissn
0143-2885issn
1365-2591journal_volume
48pub_type
杂志文章abstract:AIMS:To identify Gram-positive rods from root canals of teeth with apical periodontitis and to examine their associations with other species. METHODOLOGY:Consecutive root canal samples (RCSs) from 139 teeth undergoing root canal treatment were analyzed prospectively for cultivable microbes. Gram-positive rods in the f...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00845.x
更新日期:2004-09-01 00:00:00
abstract:AIM:To evaluate the clinical outcome of 'Lesion Sterilization and Tissue Repair' (LSTR) therapy for endodontic treatment of primary teeth. METHODOLOGY:A mixture of metronidazole, ciprofloxacin, and minocycline (3Mix) in ointment (macrogol mixed with propylene glycol: MP) or in a root canal sealer was used to disinfect...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.0143-2885.2004.00771.x
更新日期:2004-02-01 00:00:00
abstract:AIM:To compare the penetration resistance (PR) of six thermoplastic gutta-percha (GP) materials against needle penetration at different temperatures. METHODOLOGY:The PR of the following materials based on warm gutta-percha techniques, Beefill GP Cartridge (Beefill), GP Obturator-Hard/Medium/Soft (Dia-H, Dia-M, Dia-S),...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12349
更新日期:2015-06-01 00:00:00
abstract:AIM:To perform multiparametric analysis of the effects of soya milk (SM), whole milk (WM) and Hank's balanced salt solution (HBSS) on the viability of fibroblasts (HGF). The study also aimed to evaluate the influence of these solutions on bovine root dentine according to OH- and PO43- on the surface. METHODOLOGY:The H...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12739
更新日期:2017-12-01 00:00:00
abstract:AIM:To report the management of an iatrogenic incident in which an endodontic file was swallowed accidentally and passed into the gastrointestinal tract. SUMMARY:A 51-year-old male swallowed a 21 mm, size 30 endodontic file during root canal treatment without rubber dam. In the absence of clinical complications, the p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01392.x
更新日期:2008-07-01 00:00:00
abstract:AIM:To report on the use of cone beam computed tomography (CBCT) in the diagnosis and management of dens invaginatus. SUMMARY:Chronic apical periodontitis of an invaginated maxillary lateral incisor was diagnosed in a patient suffering from hypodontia and awaiting active orthodontic therapy. Loss of the tooth would ha...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01866.x
更新日期:2011-05-01 00:00:00
abstract:AIM:To describe unusual variations in the root morphology and root canal systems of mandibular first and second premolar teeth extracted for orthodontic reasons. SUMMARY:Normally mandibular first and second premolar teeth have single roots with single canals. A 15-year-old patient presented for orthodontic treatment a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01451.x
更新日期:2008-11-01 00:00:00
abstract:AIM:To examine the surfaces of a root tip removed during surgical endodontic treatment for the presence of microorganisms. SUMMARY:The present clinical case illustrates an endodontic retreatment of a maxillary premolar tooth with a fistula and periapical reaction. The case was under treatment for 1 year, during which ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00753.x
更新日期:2004-01-01 00:00:00
abstract:AIM:To evaluate the influence of post design and roughness and cement system (dual- or self-cured) on the retention of glass fibre posts. METHODOLOGY:Two tapered and smooth posts (Exacto Cônico No. 2 and White Post No. 1) and two parallel-sided and serrated posts (Fibrekor 1.25 mm and Reforpost No. 2) were adhesively ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01681.x
更新日期:2010-04-01 00:00:00
abstract:AIM:To evaluate the efficacy of a prototype root canal dressing containing surface pre-reacted glass-ionomer (S-PRG) fillers on repairing induced periapical lesions in a rat model. Calcium hydroxide [Ca(OH)2 ] was applied as a comparison in the healing process. METHODOLOGY:The pulp chambers of the maxillary first mola...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13414
更新日期:2021-02-01 00:00:00
abstract::During the past few decades several authors have perpetuated the notion that nearly half of all periapical lesions are radicular cysts. A few studies, based on meticulous serial sectioning of periapical lesions retrieved in toto, have shown that the actual incidence of radicular cyst is only about 15% of all periapica...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.1998.00146.x
更新日期:1998-05-01 00:00:00
abstract:AIM:To investigate the effects of vascular endothelial growth factor A (VEGFA) and the underlying molecular mechanisms on the migration of human dental pulp stem cells (hDPSCs). METHODOLOGY:The expression of VEGFA in inflammatory pulp tissue and lipopolysaccharide (LPS)-stimulated dental pulp cells was examined by imm...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13179
更新日期:2019-12-01 00:00:00
abstract:AIM:To determine the effect of intracoronal bleaching agents on adhesion of bonding agents to pulp chamber dentine. METHODOLOGY:Forty extracted human maxillary anterior teeth were randomly divided into four groups of 10 teeth each. Bleaching agents were sealed in pulp chambers for 7 days, as in clinical use. Group 1 (...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00931.x
更新日期:2005-04-01 00:00:00
abstract::The assurance of the quality of a service rendered by a member of the dental profession is an essential feature of any system of peer review in dentistry. This document addresses two essential elements: (i) appropriateness of treatment modality and (ii) quality or level of treatment rendered. In revising these guideli...
journal_title:International endodontic journal
pub_type: 共识发展会议,杂志文章,实务指引
doi:10.1111/j.1365-2591.2006.01180.x
更新日期:2006-12-01 00:00:00
abstract::The Netherlands Society for Endodontology has developed a 5-year plan for continuing education that served as a guideline for two basic courses in endodontology. The 2-day basic course has been attended by over 2000 dentists, and has been very much appreciated according to the evaluation outlined in this article. The ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1991.tb00131.x
更新日期:1991-07-01 00:00:00
abstract:AIM:To evaluate the effect of a glide path on the amount of apically extruded debris during canal preparation using single-file systems in curved canals. METHODOLOGY:Ninety extracted mandibular molar teeth were randomly assigned to six groups (n = 15 for each group) for canal instrumentation. Endodontic access cavitie...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12484
更新日期:2016-06-01 00:00:00
abstract::Teflon tubes containing freshly mixed, polymerized Hydron root canal filling material, fully set AH26 or Teflon were implanted into the mandible of guinea pigs and assessed histologically at 2 days, 1, 2, 4, 12 and 26 weeks. None of the materials tested elicited signs of overt or significant tissue damage, and polymer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb00749.x
更新日期:1992-07-01 00:00:00
abstract:AIM:To examine the role of stromal cell-derived factor 1 (SDF-1) signalling during odontogenic differentiation in human dental pulp cells (HDPCs). METHODOLOGY:Human dental pulp cells were treated with differentiation medium, recombinant human SDF-1, neutralizing antibody for SDF-1 or CXCR4, pertussis toxin (PTX) and A...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12182
更新日期:2014-06-01 00:00:00
abstract::In this study, an unusual observation of Saccharomyces cerevisiae isolated from an infected root canal and from the blood of a patient undergoing endodontic therapy of a tooth with asymptomatic apical periodontitis is reported. Phenotypic (biochemical tests, antifungal susceptibility tests and SDS-PAGE of cellular pro...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00101.x
更新日期:1997-09-01 00:00:00
abstract:AIM:To analyse several physicochemical properties of AH Plus (Dentsply DeTrey, Konstanz, Germany), including setting time, flow, radiopacity and the degree of conversion (DC); and to correlate the results with the source of the material: from the beginning, middle or end of the tubes in which they were supplied. METHO...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02049.x
更新日期:2012-10-01 00:00:00
abstract:AIM:To determine the root canal morphology of mandibular first premolar teeth in an Indian population using a decalcification and clearing technique. METHODOLOGY:One hundred extracted adult mandibular first premolar teeth were studied following decalcification and clearing. The shape of the canal orifice, root canal p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01494.x
更新日期:2009-01-01 00:00:00
abstract:AIM:To investigate the use of a zinc oxide/zinc sulphate-based cement (Coltosol(®) F, Coltène Whaledent, Cuyahoga Falls, OH, USA) as a temporary filling material during multiple-visit root canal treatments and the occurrence of cracks within the filling material or the tooth. METHODOLOGY:Root canals of one hundred and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12280
更新日期:2015-01-01 00:00:00
abstract:AIM:To develop a precise and simple mounting model (MM) for evaluating apex locators and to compare the repeatability of the MM with a conventional visual method (VM). METHODOLOGY:Electronic working length determination was performed in 32 maxillary central incisors using two methods: (i) the MM method and (ii) a conv...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01052.x
更新日期:2006-02-01 00:00:00
abstract:AIM:To evaluate the radiopacity of calcium hydroxide-based root canal sealers (Acroseal, Sealapex and Sealer 26), a glass-ionomer-based sealer (Activ GP Sealer) and a zinc oxide and eugenol-based sealer (Intrafill). METHODOLOGY:Five disc-shaped specimens (10 x 1 mm) were fabricated from each material, according to the...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01309.x
更新日期:2008-01-01 00:00:00
abstract:AIM:To evaluate the torsional properties of the NiTi glide path reciprocating instruments R-Pilot (VDW, Munich, Germany) and WaveOne Gold Glider (Dentsply Sirona, Ballaigues, Switzerland). METHODOLOGY:Ten R-Pilot (size 12.5, .04 taper) and 10 WaveOne Gold Glider (size 15, .02v taper) instruments were used. The torque ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13068
更新日期:2019-06-01 00:00:00
abstract:AIM:To develop a convenient method for the localization and quantification of live and dead bacteria in human ex vivo mineralized dentinal tubules. METHODOLOGY:The roots from human single-rooted teeth (n = 12) were infected with Enterococcus faecalis V583 and either treated with calcium hydroxide paste or left untreat...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01867.x
更新日期:2011-07-01 00:00:00
abstract:AIM:To evaluate the resistance to ex vivo bacterial leakage over a 40-day period of root canal fillings with five new root canal sealers: RC Sealer, Epiphany, EndoREZ, GuttaFlow and Acroseal, compared with Apexit, AH Plus and RoekoSeal. METHODOLOGY:One hundred and forty-four single rooted human teeth were divided rand...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01509.x
更新日期:2009-04-01 00:00:00
abstract::The aim of this study was to systematically review and critically analyse the published data on the treatment outcome (primary outcome) and on the cleaning and disinfection of root canals (secondary outcomes) achieved by negative pressure irrigation as compared to syringe irrigation. An electronic search was conducted...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12725
更新日期:2017-11-01 00:00:00
abstract:AIM:To compare the incidence of dentinal defects caused by reciprocating and rotary techniques during retreatment procedures. METHODOLOGY:One hundred and twenty extracted mandibular premolars with single canals were selected. Twenty teeth were left unprepared. The root canals in the remaining 100 teeth were prepared w...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12387
更新日期:2015-10-01 00:00:00
abstract::Dentoalveolar ankylosis is a common complication after replantation of teeth with injured periodontal ligaments. This is a serious complication for the tooth since it leads to progressive root resorption. In young individuals it may also cause infra-occlusion and reduced development of the alveolar process. The purpos...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00549.x
更新日期:1993-03-01 00:00:00