Abstract:
AIM:To develop a convenient method for the localization and quantification of live and dead bacteria in human ex vivo mineralized dentinal tubules. METHODOLOGY:The roots from human single-rooted teeth (n = 12) were infected with Enterococcus faecalis V583 and either treated with calcium hydroxide paste or left untreated; six control roots were uninoculated and untreated. Following further incubation, roots were stained with fluorescent DNA-binding reagents, washed thoroughly, sectioned and examined by confocal laser scanning microscopy. Computer-assisted determinations of fluorescence (bacterial viability) were compared statistically. RESULTS:Bacteria were distributed in the tubules throughout the length of the roots but tubule penetration distance was slightly reduced in the apical sections. There was no significant difference in bacterial tubule penetration between roots from different teeth and small standard deviations indicated reproducibility appropriate for experimental application. Following treatment with calcium hydroxide paste, live and dead bacteria were readily distinguishable by contrasting green and red fluorescence. Bacterial viability determinations amongst roots treated in the same way were not significantly different, and the small standard deviation is commensurate with experimental application. CONCLUSIONS:Fluorescent viability staining is a convenient, accurate and reproducible method for localizing and quantifying live and dead bacteria in human ex vivo mineralized dentinal tubules.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Parmar D,Hauman CH,Leichter JW,McNaughton A,Tompkins GRdoi
10.1111/j.1365-2591.2011.01867.xsubject
Has Abstractpub_date
2011-07-01 00:00:00pages
644-51issue
7eissn
0143-2885issn
1365-2591journal_volume
44pub_type
杂志文章abstract:AIM:This study was designed to examine whether enamel matrix derivative (EMD) could induce reparative dentine formation without eliciting adverse side-effects in pulpotomized teeth in the miniature swine. METHODOLOGY:Pulpotomy was performed in 36 mandibular incisor teeth from 11 adult miniature swine. Following the su...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00556.x
更新日期:2002-05-01 00:00:00
abstract:AIM:To report the use of cone beam computed tomography in the assessment of external cervical resorption lesions. SUMMARY:Asymptomatic external cervical resorption lesions were diagnosed radiographically in two patients. Clinical examination in both cases was unremarkable. Cone beam computed tomography scans revealed ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01247.x
更新日期:2007-09-01 00:00:00
abstract::Thorough knowledge of anatomical complexities of the root canal system has a direct impact on the effectiveness of canal preparation and filling, and is an essential prerequisite for successful root canal treatment. A wide range of complex variations in root canal anatomy exists, including root canal configuration typ...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12800
更新日期:2018-02-01 00:00:00
abstract:CASE REPORTS:The problem of thermal sensitivity following non-surgical root-canal treatment is explored and case reports are presented. Possible causes for post-treatment discomfort from endodontic and restorative aetiologies are discussed, as are the mechanisms to explain the patients' painful experiences. Treatment o...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00197.x
更新日期:1999-03-01 00:00:00
abstract::The aim of this in vitro study was to determine the effect of removal of the smear layer on canal obturation as measured by penetration of bacteria from a coronal direction. One hundred and twenty extracted human teeth with straight, single root canals were decoronated. The canals were prepared using the modified doub...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1996.tb01376.x
更新日期:1996-07-01 00:00:00
abstract:AIM:To compare 2D with 3D radiography in assessing the treatment outcome 1 year after periapical surgery. METHODOLOGY:In this prospective study, periapical radiographs (PA) and cone beam computed tomography (CBCT) were performed 1 year after periapical surgery. Three calibrated observers independently evaluated the ra...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12548
更新日期:2016-10-01 00:00:00
abstract:AIM:To present a scientometric analysis of the entire body of scientific publications in the field of vital pulp therapy (VPT) and analyse the research trends and popular topics. METHODOLOGY:A comprehensive literature search was conducted in the Web of Science and Scopus databases on 21 August 2020 to identify all art...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13422
更新日期:2021-02-01 00:00:00
abstract::Chelating agents were introduced into endodontics as an aid for the preparation of narrow and calcified root canals in 1957 by Nygaard-Østby. A liquid solution of ethylenediaminetetraacetic acid (EDTA) was thought to chemically soften the root canal dentine and dissolve the smear layer, as well as to increase dentine ...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2591.2003.00754.x
更新日期:2003-12-01 00:00:00
abstract::A case is described in which root canal treatment with calcium hydroxide was used successfully to repair a fracture site with internal resorption of the tooth. ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1996.tb01404.x
更新日期:1996-11-01 00:00:00
abstract:AIM: To discuss the clinical and radiographic success of a pulpotomy with second-generation platelet concentrate (PRF), in a human mature permanent molar tooth. SUMMARY: A 19-year-old female patient reported to the Department of Conservative Dentistry and Endodontics with established pulpitis in tooth 36. The tooth ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01973.x
更新日期:2012-01-01 00:00:00
abstract:AIM:To investigate the incidence of and factors associated with pulpal necrosis in vital teeth restored with metal-ceramic crowns (CMCs) or crowned as part of a fixed-fixed bridge. METHODOLOGY:Patients who had a CMC or bridge retainer (BR) placed on a tooth with no previous history of root canal treatment from 1981 to...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.00982.x
更新日期:2005-08-01 00:00:00
abstract:AIM:To evaluate the influence of delayed scanning on images obtained with two PSPs digital systems and on the diagnostic accuracy of vertical root fracture (VRF) by means of objective and subjective analyses. METHODOLOGY:Forty single-rooted human teeth were divided into two groups, one without VRFs and another with VR...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12547
更新日期:2016-10-01 00:00:00
abstract:AIM:To investigate the molecular mechanisms of nitric oxide (NO)-induced cytotoxic effect in human gingival fibroblast (HGF) cells. METHODOLOGY:After sodium nitroprusside (SNP), as NO donor, was treated to HGF, viability was measured by MTT assay and apoptosis was determined by TUNEL and DNA fragmentation assay. Mitoc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12314
更新日期:2015-03-01 00:00:00
abstract:AIM:To assess the effect of acid-etching duration time on the compressive strength, microstructure and shear bond strength of All-Bond Universal adhesive to MTA-Angelus (AMTA), and Biodentine (BD). METHODOLOGY:Forty-eight cylindrical specimens (4 mm in diameter, 6 mm in height) of AMTA in four groups and 48 specimens ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13026
更新日期:2019-04-01 00:00:00
abstract:AIM:To establish a cell line of immortalized human dental papilla cells (hDPCs). METHODOLOGY:Primary hDPCs were cultured and infected with lentivirus containing the hTERT gene. Integration and transcription of the hTERT gene were verified by PCR. The characteristics of the cells, such as morphology, proliferation and ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12029
更新日期:2013-06-01 00:00:00
abstract:AIM:Apatite-forming ability, considered as an index of bioactivity (bond-to-bone ability), was tested on ProRoot MTA cement after immersion in phosphate-containing solution (DPBS). METHODOLOGY:Disk samples were prepared and immersed in DPBS for 10 min, 5 h, 1 and 7 days. The cement surface was studied by attenuated to...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01768.x
更新日期:2010-10-01 00:00:00
abstract::The aim of the present systematic review was to evaluate the effectiveness of technology-enhanced learning (TEL) in the field of Endodontics to improve educational outcomes compared to traditional learning methods. Randomized controlled studies published in English were identified from two electronic databases (PubMed...
journal_title:International endodontic journal
pub_type: 杂志文章,meta分析
doi:10.1111/iej.12995
更新日期:2019-02-01 00:00:00
abstract::When operated in air, the sonic endodontic instrument produced a movement at the driver head which resulted in a large elliptical oscillation of the file. This motion had similar properties to ultrasonically operated files in that there was a distinct variability in the magnitude of the vibratory motion between files ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1989.tb00910.x
更新日期:1989-05-01 00:00:00
abstract:AIM:To establish and characterize different types of fibroblastic cell lines derived from dental pulp tissue. METHODOLOGY:Human dental pulp tissue-derived cells were transfected with SV40 large T antigen by Lipofectamine transfection method. Geneticin (G418)-resistant cells were selected and different cell lines were ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01409.x
更新日期:2008-07-01 00:00:00
abstract:AIM:To examine the clinical and radiographic appearance of teeth that suffered premature interruption of root development and were treated by an mineral trioxide aggregate (MTA) apical plug technique. SUMMARY:Eleven teeth with immature root apices in 11 patients were treated nonsurgically by the manual application of ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01240.x
更新日期:2007-06-01 00:00:00
abstract:AIM:To compare in vitro the cleanliness of root canal walls in oval-shaped root canals following automated or manual instrumentation. METHODOLOGY:Forty-five oval-shaped single-rooted maxillary and mandibular premolars with straight canals were divided into three groups of 15. Automated canal preparation was performed ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.00938.x
更新日期:2005-06-01 00:00:00
abstract:AIM:To compare the effect of 5.25% NaOCl or 17% EDTA solutions on the surface topography and roughness of GTX, TF, RaCe and Hero Shaper instruments using atomic force microscopy (AFM) analysis. METHODOLOGY:Four new files of each brand were subjected to AMF analysis; then each group was subdivided into two subgroups (n...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12189
更新日期:2014-06-01 00:00:00
abstract:AIM:To determine the influence of various irrigating solutions on the accuracy of the electronic apex locator facility in the Tri Auto ZX handpiece. METHODOLOGY:One hundred and forty teeth with single canals and mature apices, scheduled for extraction for either periodontal or prosthetic reasons in 76 patients were us...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2007.01234.x
更新日期:2007-05-01 00:00:00
abstract:AIM:To assay the toxicity of the single-methacrylate-based sealer urethane dimethacrylate (UDMA) (EndoRez) in terms of cell growth and pro-inflammatory cytokines release, in expanded ex vivo human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPDLSCs), human gingival fibroblasts (hGFs) and hum...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12230
更新日期:2014-09-01 00:00:00
abstract:AIM:To determine retrospectively the long-term radiographic outcome of root canal treatments and root canal retreatments with unintentional root canal overfilling. METHODOLOGY:A total of 220 root canal treatments (143 primary/77 retreatments) with postoperative unintentional canal overfilling and performed by two endo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13209
更新日期:2020-01-01 00:00:00
abstract::Teflon tubes containing freshly mixed, polymerized Hydron root canal filling material, fully set AH26 or Teflon were implanted into the mandible of guinea pigs and assessed histologically at 2 days, 1, 2, 4, 12 and 26 weeks. None of the materials tested elicited signs of overt or significant tissue damage, and polymer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb00749.x
更新日期:1992-07-01 00:00:00
abstract:AIM:To evaluate the physical and mechanical properties of two cements with a similar chemical composition to mineral trioxide aggregate and to attempt to improve their properties by producing a composite material. METHODOLOGY:Two cement types were used: a mixture of calcium sulpho-aluminate cement and Portland cement ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01435.x
更新日期:2008-10-01 00:00:00
abstract:AIM:To evaluate and compare the push-out bond strength of MTA Fillapex (Angelus, Londrina, Brazil) and iRoot SP (Innovative BioCeramix Inc., Vancouver, Canada) to the dentine walls of root canals. AH Plus (Dentsply DeTrey, Konstaz, Germany) and MTA (Angelus, Londrina, Brazil) were used as reference materials. METHODOL...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12519
更新日期:2016-08-01 00:00:00
abstract:AIM:To investigate the smallest dentine thickness in mesial canals of mandibular molars along the cervical and middle thirds of the root by means of a micro-computed tomographic (micro-CT) technology and digital image analysis. METHODOLOGY:Fifty mesial roots of mandibular molars having two independent canals (mesiobuc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13141
更新日期:2019-10-01 00:00:00
abstract::The large variations observed in dentists' management of periapical lesions in endodontically treated teeth suggest that disease concepts used in clinical practice should be investigated. In the present study it was hypothesized that dentists regard various periapical conditions as different stages on a health continu...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00270.x
更新日期:1994-11-01 00:00:00