Abstract:
AIM:To establish a cell line of immortalized human dental papilla cells (hDPCs). METHODOLOGY:Primary hDPCs were cultured and infected with lentivirus containing the hTERT gene. Integration and transcription of the hTERT gene were verified by PCR. The characteristics of the cells, such as morphology, proliferation and mineralization, were analysed. Also, the expression of odontoblastic-related markers including ALP, DMP1, DLX3, OSX, DSP and Nestin, was detected by immunohistochemistry and real-time RT-PCR. RESULTS:hTERT gene was integrated into genomic DNA of immortalized cells (hDPC-TERT) and transcribed into mRNA. With long-time culture, hDPC-TERT bypassed senescence and grew over 120 population doublings. hDPC-TERT cells have a higher proliferation rate, but retain the phenotypic characteristics of the primary hDPCs, and so was ALP activity and mineralization activity. Furthermore, the hDPC-TERT cells express no DSP and Nestin with maintenance medium, but highly expressed DSP and Nestin after odontoblastic induction. CONCLUSIONS:A line of immortalized human dental papilla cells, which remains in an undifferentiated state and has odontoblastic differentiation potential, was established. This cell line can be used as a cell model for studying the mechanism of the initiation of odontoblast differentiation.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Yang GB,Li XY,Yuan GH,Liu H,Fan MWdoi
10.1111/iej.12029subject
Has Abstractpub_date
2013-06-01 00:00:00pages
565-72issue
6eissn
0143-2885issn
1365-2591journal_volume
46pub_type
杂志文章abstract:AIM:To determine retrospectively the long-term radiographic outcome of root canal treatments and root canal retreatments with unintentional root canal overfilling. METHODOLOGY:A total of 220 root canal treatments (143 primary/77 retreatments) with postoperative unintentional canal overfilling and performed by two endo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13209
更新日期:2020-01-01 00:00:00
abstract:AIM:To evaluate the response of the apical and periapical tissues of dog teeth with apical periodontitis after one-session root canal treatment with and without antimicrobial photodynamic therapy (aPDT) compared with the use of an intracanal dressing. METHODOLOGY:Sixty root canals with an induced periapical lesion wer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12486
更新日期:2016-06-01 00:00:00
abstract::The aim of this report is to (i) review the current literature on the status of root filled teeth, (ii) analyse the most important factors in decision-making, (iii) discuss the current restorative concepts, and (iv) classify both the evidence and clinical practice in a way that seeks to be clear, understandable and he...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12847
更新日期:2018-03-01 00:00:00
abstract:AIM:To evaluate the antimicrobial efficacy of Ocimum sanctum, Cinnamomum zeylanicum, Syzygium aromaticum and 3% sodium hypochlorite (NaOCl) against Enterococcus faecalis in planktonic suspension and biofilm phenotypes. METHODOLOGY:The antibacterial efficacy of different concentrations of aqueous ethanolic extracts of ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12058
更新日期:2013-08-01 00:00:00
abstract::Following extraction of a symptomatic mandibular premolar which had been subjected to two periradicular surgical procedures, significant apical healing was identified histologically. The implications of these findings are discussed in relation to contemporary advocated treatment regimens, case assessment, and interpre...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00280.x
更新日期:1994-11-01 00:00:00
abstract::Sodium hypochlorite (NaOCl), is the most popular irrigation solution used in root canal treatment but it is known to be an irritant to vital tissues. There have been many case reports dealing with toxic effects of this material. Although the allergic potential of NaOCl has been described in the medical literature, onl...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00247.x
更新日期:1994-05-01 00:00:00
abstract:AIM:This randomized, double-blinded, clinical trial evaluated the effect of oral premedication of piroxicam, prednisolone, dexamethasone or placebo on postoperative pain after single-visit root canal treatment in teeth with symptomatic irreversible pulpitis and symptomatic apical periodontitis. METHODOLOGY:The trial i...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/iej.13416
更新日期:2021-02-01 00:00:00
abstract:AIM:To evaluate the radiopacity of calcium hydroxide-based root canal sealers (Acroseal, Sealapex and Sealer 26), a glass-ionomer-based sealer (Activ GP Sealer) and a zinc oxide and eugenol-based sealer (Intrafill). METHODOLOGY:Five disc-shaped specimens (10 x 1 mm) were fabricated from each material, according to the...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01309.x
更新日期:2008-01-01 00:00:00
abstract:AIM:The aim of this study was to test the null hypothesis that sodium hypochlorite irrigation of root canals does not alter the properties of dentine and contribute to the weakening of root-treated teeth. METHODOLOGY:The effect of two concentrations (0.5%, 5.25%) of sodium hypochlorite (NaOCl) and saline on (i) the el...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2001.00357.x
更新日期:2001-03-01 00:00:00
abstract:AIM:To assess the inflammatory potential of biofilm matrix constituents of Enterococcus faecalis and Pseudomonas aeruginosa monospecies biofilms on macrophages. METHODOLOGY:In vitro biofilms of E. faecalis and P. aeruginosa were grown (7 days) in aerobic and anaerobic conditions. The biofilm matrix components: exopoly...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13093
更新日期:2019-07-01 00:00:00
abstract::Thorough knowledge of anatomical complexities of the root canal system has a direct impact on the effectiveness of canal preparation and filling, and is an essential prerequisite for successful root canal treatment. A wide range of complex variations in root canal anatomy exists, including root canal configuration typ...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12800
更新日期:2018-02-01 00:00:00
abstract:AIM:To compare the efficacy of reciprocating and rotary techniques with that of hand files for removing gutta-percha and sealer from root canals. METHODOLOGY:The root canals of fifty-four human extracted maxillary central incisors were cleaned and shaped using a crown-down technique to a size 40 and filled with gutta-...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12085
更新日期:2013-10-01 00:00:00
abstract:AIM:To assess the long-term outcome of root filled teeth in a general adult population examined in 1997, 2003 and 2008. METHODOLOGY:The study provides longitudinal, observational information on endodontic status for 327 randomly selected individuals from a general Danish population receiving three consecutive full-mou...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12245
更新日期:2014-10-01 00:00:00
abstract:AIM:To compare the area occupied by gutta-percha, sealer, or void in standardized .06 tapered prepared simulated curved canals and in mesio-buccal canals of extracted maxillary first molars filled with a single .06 gutta-percha point and sealer or lateral condensation of multiple .02 gutta-percha points and sealer. ME...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00903.x
更新日期:2005-02-01 00:00:00
abstract:AIM:To evaluate the effect of repeated sterilization cycles in dry oven or autoclave, on the mechanical behaviour and fatigue resistance of rotary endodontic Ni-Ti instruments. METHODOLOGY:New Ni-Ti instruments were subjected to five consecutive sterilization cycles in a dry oven or steam autoclave. Microhardness was ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01138.x
更新日期:2006-09-01 00:00:00
abstract::A case is described in which root canal treatment with calcium hydroxide was used successfully to repair a fracture site with internal resorption of the tooth. ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1996.tb01404.x
更新日期:1996-11-01 00:00:00
abstract:AIM:The aim of this study was to determine the shaping ability of GT Rotary Files in simulated root canals. METHODOLOGY:Forty canals with four different shapes in terms of angle (40 degrees and 60 degrees) and position of curvature (straight section before curve: 8 and 12 mm) were prepared using a crown-down/stepback ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00540.x
更新日期:2002-07-01 00:00:00
abstract::In this study, an unusual observation of Saccharomyces cerevisiae isolated from an infected root canal and from the blood of a patient undergoing endodontic therapy of a tooth with asymptomatic apical periodontitis is reported. Phenotypic (biochemical tests, antifungal susceptibility tests and SDS-PAGE of cellular pro...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00101.x
更新日期:1997-09-01 00:00:00
abstract:AIM:To evaluate ex vivo the long-term sealing ability of the SE Resilon Epiphany system as an apical root-end filling material. METHODOLOGY:A total of 60 standardized horizontal bovine root sections were divided into three groups filled with either gutta-percha with AH 26, tooth-coloured mineral trioxide aggregate (MT...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01583.x
更新日期:2009-10-01 00:00:00
abstract:AIM:The aim of the present study was to investigate the antimicrobial activity of octenidine on Enterococcus faecalis ATCC 29212 in a dentine block model. METHODOLOGY:Fifty-six root segments of extracted human teeth were infected with E. faecalis for 4 weeks. Octenidine-phenoxyethanol gel (1 : 1) was applied for diffe...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01279.x
更新日期:2007-11-01 00:00:00
abstract:AIM:To investigate the incidence of and factors associated with pulpal necrosis in vital teeth restored with metal-ceramic crowns (CMCs) or crowned as part of a fixed-fixed bridge. METHODOLOGY:Patients who had a CMC or bridge retainer (BR) placed on a tooth with no previous history of root canal treatment from 1981 to...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.00982.x
更新日期:2005-08-01 00:00:00
abstract:AIM:To report the management of an iatrogenic incident in which an endodontic file was swallowed accidentally and passed into the gastrointestinal tract. SUMMARY:A 51-year-old male swallowed a 21 mm, size 30 endodontic file during root canal treatment without rubber dam. In the absence of clinical complications, the p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01392.x
更新日期:2008-07-01 00:00:00
abstract:AIM:The Bramante method was used to study how the natural curvature of root canals was modified when using the Hero 642 nickel-titanium rotary system. METHODOLOGY:Twenty-four canals were selected in freshly extracted human mandibular molars. Every root canal had an angle of curvature greater than 20 degrees. Roots wer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2001.00440.x
更新日期:2001-12-01 00:00:00
abstract:AIM:The aim of this study was to determine the ability of the Quantec Series 2000 rotary nickel-titanium endodontic system to remove dentinal debris and smear layer produced during canal preparation. METHODOLOGY:A first group (control) of 12 curved root canals was prepared using conventional manual instruments and the...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00231.x
更新日期:1999-05-01 00:00:00
abstract:AIM:To investigate the role of the Ca(2+) -Mg(2+) ion channel TRPM7 in the proliferation, migration and osteogenic differentiation of human dental pulp stem cells (hDPSCs). METHODOLOGY:Immunohistochemistry was used to localize expression of TRPM7 in human dental pulp tissues and in cultured hDPSCs. Isolated hDPSCs wer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12193
更新日期:2014-06-01 00:00:00
abstract:AIM:To determine the bacterial microbiota in root canals associated with persistent apical periodontitis and their relationship with the clinical characteristics of patients using next-generation sequencing (NGS). METHODOLOGY:Bacterial samples from root canals associated with teeth having persistent apical periodontit...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12953
更新日期:2018-12-01 00:00:00
abstract:AIM:The aims of this study were firstly to compare the area of canal occupied by gutta-percha, sealer or voids using the System B heating device with that obtained by a modified vertical condensation technique using the Touch 'n Heat: and secondly to compare the temperature changes at the root canal wall and external r...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00215.x
更新日期:1999-08-01 00:00:00
abstract:AIM:To evaluate the adhesive strength of Resilon to Next root canal sealant (Heraeus-Kulzer), a methacrylate-based root canal sealer, using a modified microshear bond testing design. METHODOLOGY:Flat Resilon surfaces of different roughnesses (smooth surface and surface roughness equivalent to 320-grit and 180-grit) we...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.01012.x
更新日期:2005-10-01 00:00:00
abstract:AIM:To investigate in vitro the cytocompatibility of the calcium silicate-containing endodontic sealers MTA Fillapex and TotalFill BC Sealer on human periodontal ligament stem cells (hPDLSCs) by assaying their biological responses and compare them with that observed when using an epoxy resin-based sealer (AH Plus). ME...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12596
更新日期:2017-01-01 00:00:00
abstract::This systematic review (SR; PROSPERO database: CRD42017075160) and network meta-analysis (NMA) identified the most effective oral premedication for anaesthetic success of inferior alveolar nerve blocks (IANB) in cases of irreversible pulpitis. Medline and Ebscohost databases were searched up until 10/2017. Randomized ...
journal_title:International endodontic journal
pub_type: 杂志文章,meta分析
doi:10.1111/iej.12912
更新日期:2018-09-01 00:00:00