Abstract:
AIM:To assess the inflammatory potential of biofilm matrix constituents of Enterococcus faecalis and Pseudomonas aeruginosa monospecies biofilms on macrophages. METHODOLOGY:In vitro biofilms of E. faecalis and P. aeruginosa were grown (7 days) in aerobic and anaerobic conditions. The biofilm matrix components: exopolysaccharides (EPS) and extracellular DNA (eDNA) were extracted and quantified. The inflammatory potential of EPS and eDNA was assessed on macrophage cell lines (RAW 267.4) using nitric oxide (NO), and enzyme-linked immunosorbent assay for tumour necrosis factor (TNF-α) and interleukin-6 (IL-6) expressions. LPS from P. aeruginosa and planktonic bacteria were positive controls. One-way analysis of variance and the Tukey post hoc test were used for statistical analysis. RESULTS:Extracted EPS from both biofilm strains was associated with higher levels than eDNA in both growth conditions (P < 0.05). The biofilm components had less inflammatory potential compared to planktonic bacteria and LPS. EPS produced higher levels of inflammatory response compared to eDNA for both strains (P < 0.05). IL-6 and TNF-α, and NO expression showed no difference for E. faecalis EPS (P ≥ 0.05). In contrast, P. aeruginosa EPS and eDNA had significant levels of IL-6 compared to TNF-α and NO (P < 0.05). CONCLUSIONS:Monospecies biofilm matrix EPS and eDNA from the bacterial strains tested had the ability to induce a low-grade inflammatory response when compared to planktonic bacteria and LPS. This study highlights the potential of biofilm matrix/components, devoid of bacteria to induce low-grade chronic inflammation.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Ramirez T,Shrestha A,Kishen Adoi
10.1111/iej.13093subject
Has Abstractpub_date
2019-07-01 00:00:00pages
1020-1027issue
7eissn
0143-2885issn
1365-2591journal_volume
52pub_type
杂志文章abstract:AIM:To evaluate the therapeutic effect of Simvastatin on apical periodontitis in rats by assessing the osteoblast production of receptor activator of nuclear factor-Kappa B Ligand (RANKL) and Osteoprotegerin (OPG) cytokines that are essential for bone resorption. METHODOLOGY:Twenty-five healthy 8- to 10-week-old male ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12101
更新日期:2013-11-01 00:00:00
abstract:AIM:To evaluate the effect of multiple refreshment/activation cycles and temperature on the reaction rate of sodium hypochlorite (NaOCl) with bovine dentine during ultrasonic activated irrigation (UAI) under laboratory conditions. METHODOLOGY:The root canal walls of 24 standardized root canals in bovine incisors were ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12125
更新日期:2014-02-01 00:00:00
abstract:AIM:To compare periapical health using samples from prehistoric and historic periods until the present day, and to emphasize the major risk indicators for apical periodontitis (AP). METHODOLOGY:A comparative survey to assess periapical health was performed on five samples (525 individuals) drawn from different periods...
journal_title:International endodontic journal
pub_type: 历史文章,杂志文章
doi:10.1111/j.1365-2591.2005.00905.x
更新日期:2005-05-01 00:00:00
abstract:AIM:To determine retrospectively the long-term radiographic outcome of root canal treatments and root canal retreatments with unintentional root canal overfilling. METHODOLOGY:A total of 220 root canal treatments (143 primary/77 retreatments) with postoperative unintentional canal overfilling and performed by two endo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13209
更新日期:2020-01-01 00:00:00
abstract:AIM:To evaluate the effect of rotational speed and the angle and radius of curvature of root canals on the fracture of two types of nickel-titanium rotary instruments: K3 and ProTaper. METHODOLOGY:A total of 240 root canals of extracted human maxillary and mandibular molars were divided into two groups of 120, accordi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00630.x
更新日期:2003-04-01 00:00:00
abstract:AIM:To report the hydration mechanism of white mineral trioxide aggregate (White MTA, Dentsply, Tulsa Dental Products, Tulsa, OK, USA). METHODOLOGY:The chemical constitution of white MTA was studied by viewing the powder in polished sections under the scanning electron microscope (SEM). The hydration of both white MTA...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01248.x
更新日期:2007-06-01 00:00:00
abstract:AIM:To assess and correlate three distinct states of severely painful endodontically derived inflammation with their depiction on periapical radiographs using periapical index (PAI) scores. METHODOLOGY:During a period of 15 months, 368 consecutively enrolled patients with suspected endodontic emergency conditions were...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13407
更新日期:2021-02-01 00:00:00
abstract:AIM:To use micro-computed tomography (μ-CT) to evaluate the amount of calcium hydroxide [Ca(OH)2 ] remaining in the C-shaped root canals of mandibular second molars after attempting to remove it with passive ultrasonic and sonic irrigation. METHODOLOGY:Thirty mandibular second molars, 15 in C1 and 15 in C2 configurati...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12319
更新日期:2015-04-01 00:00:00
abstract:AIMS:To (i) determine the prevalence of persistent dento-alveolar pain following nonsurgical and/or surgical endodontic treatment conducted in a teaching dental hospital and (ii) identify the risk factors associated with persistent pain after apparently successful root canal treatment. STUDY DESIGN:A total of 175 pati...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00923.x
更新日期:2005-03-01 00:00:00
abstract:AIM:To evaluate the effects of a long period of oestrogen deficiency on the development of apical periodontitis in rats. METHODOLOGY:Wistar rats (n = 24), 3 months old, evaluated by vaginal cytology, were included in the study. Twelve animals were ovariectomized (OVX group) and the other 12 were sham operated (control...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12612
更新日期:2017-02-01 00:00:00
abstract:AIM:To detect and quantify dentine sialoprotein (DSP) in the gingival crevicular fluid (GCF) of luxated teeth. METHODOLOGY:Eighteen subjects were enroled and distributed as follows. Group I (n = 6, positive control): subjects with primary second molar teeth undergoing physiological root resorption. Group II (n = 6, ne...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12001
更新日期:2013-04-01 00:00:00
abstract:AIM:To compare torque and angle of rotation at fracture of new and used K3 rotary instruments. The relation between size of instrument and torque at fracture was also investigated. METHODOLOGY:Nickel-titanium (Ni-Ti).06 K3 rotary instruments were used in a crown-down manner at 300 r.p.m. to prepare canals in resin blo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00732.x
更新日期:2003-11-01 00:00:00
abstract:AIM:To establish a cell line of immortalized human dental papilla cells (hDPCs). METHODOLOGY:Primary hDPCs were cultured and infected with lentivirus containing the hTERT gene. Integration and transcription of the hTERT gene were verified by PCR. The characteristics of the cells, such as morphology, proliferation and ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12029
更新日期:2013-06-01 00:00:00
abstract:AIM:To describe some toxic effects of arsenic trioxide in the mouth, to condemn its continued use, and present a case in which a tooth was preserved despite significant bony destruction. SUMMARY:A case is presented in which severe alveolar bone necrosis resulted from leak-age of an arsenical devitalization paste into ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00491.x
更新日期:2002-03-01 00:00:00
abstract:AIM:To evaluate lymphocyte-like cell activation (CD5-positive cells) and the expression of interleukin (IL)-6 and IL-17 in the pulp after tooth bleaching with two concentrations of hydrogen peroxide (H2 O2 ). METHODOLOGY:The right and left maxillary molars from 40 rats were treated randomly with bleaching gel with 20%...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12891
更新日期:2018-07-01 00:00:00
abstract:AIM:To describe the use of a new protective device to reduce aerosol dispersion in dental clinics during the COVID-19 pandemic. METHODOLOGY:The device consists of a rigid translucent acrylic structure (methyl polymethacrylate), adjusted to the dental chair, involving the patient's head, neck and chest. There is also a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13373
更新日期:2020-11-01 00:00:00
abstract:AIM:This randomized, double-blind, placebo-controlled, clinical trial was designed to improve the success of inferior alveolar nerve blocks (IANB) in mandibular molars with symptomatic irreversible pulpitis (SIP) by means of preoperative submucosal administration of 50 mg tramadol. METHODOLOGY:Forty-two patients with ...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/iej.12755
更新日期:2017-12-01 00:00:00
abstract:AIM:To analyse responses from dental practitioners (DPs) on how secure they felt as a newly graduated dentist, level of confidence or self-efficacy when performing root canal treatment (RCT), and if undergraduate (UG) education in Endodontics adequately met their needs in a dental practice. METHODOLOGY:An electronic q...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13455
更新日期:2020-11-30 00:00:00
abstract::The large variations observed in dentists' management of periapical lesions in endodontically treated teeth suggest that disease concepts used in clinical practice should be investigated. In the present study it was hypothesized that dentists regard various periapical conditions as different stages on a health continu...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00270.x
更新日期:1994-11-01 00:00:00
abstract:AIM:The aim of the present study was to identify periapical changes 20-27 years after root-canal treatment. METHODOLOGY:The periapical condition of 265 roots filled by undergraduate students was evaluated in two series of intraoral radiographs taken 10-17 and 20-27 years after treatment. Roots (72) not recorded with a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00568.x
更新日期:2002-09-01 00:00:00
abstract::Four hundred and fifty-five extracted mandibular incisor and 340 extracted mandibular premolar teeth were radiographed to assess the incidence of twin canals as visualized on radiographs taken in the mesio-distal direction. The ability to detect the presence of these twin canals by viewing radiographs taken in the sta...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1991.tb00808.x
更新日期:1991-03-01 00:00:00
abstract:AIM:To describe the successful non-surgical endodontic management of a permanent maxillary lateral incisor tooth with four root canals. SUMMARY:Aberrant morphology of root canals can occur in any tooth, including maxillary lateral incisors. In the presented case, clinical examination and radiographs clearly depicted t...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01984.x
更新日期:2012-04-01 00:00:00
abstract:AIM:To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. METHODOLOGY:A total of 50 human periodontal ligament samples were obtained from health...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12952
更新日期:2018-12-01 00:00:00
abstract:AIM:To compare dentinal microcrack formation whilst using Ni-Ti hand K-files, ProTaper hand and rotary files and the WaveOne reciprocating file. METHODOLOGY:One hundred and fifty mandibular first molars were selected. Thirty teeth were left unprepared and served as controls, and the remaining 120 teeth were divided in...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12197
更新日期:2014-07-01 00:00:00
abstract::The cutting heads of the first 18 instruments in six sets of clinically used Lightspeed instruments (sizes 20-65) were examined in a scanning electron microscope (SEM). Three sets had been used by full-time faculty and discarded as follows: instrument sizes 20-32.5 after 18 treated root canals; instrument sizes 35-47....
journal_title:International endodontic journal
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:AIM:To evaluate and compare the canal shaping ability of BioRace, ProTaper NEXT and Genius engine-driven nickel-titanium (NiTi) file systems in extracted mandibular first molars using micro-computed tomography (MCT). METHODOLOGY:Sixty mesial root canals of mandibular first molars were randomly divided into three equal...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12961
更新日期:2019-01-01 00:00:00
abstract:AIM:To evaluate the effect of depth of insertion of an irrigation needle tip on the removal of hard-tissue debris using micro-computed tomographic (micro-CT) imaging. METHODOLOGY:Twenty isthmus-containing mesial roots of mandibular molars were anatomically matched based on similar morphological dimensions using micro-...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12648
更新日期:2017-06-01 00:00:00
abstract:AIM:To evaluate the biocompatibility of two pastes designed to fill the root canals of primary teeth. METHODOLOGY:A study group of 54 mice received subcutaneous tissue implants of polyethylene tubes containing CTZ or calcium hydroxide paste or, as a negative control, empty tubes. Biocompatibility was evaluated on days...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12328
更新日期:2015-05-01 00:00:00
abstract:AIM:To elucidate the expressions of MMP-8 and MMP-13 in experimentally induced rat periradicular lesions by means of the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining. METHODOLOGY:Thirty rats were used and periradicular lesions in mandibular first molar teeth were establishe...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01880.x
更新日期:2011-08-01 00:00:00
abstract:AIM:To report clinical complications (pain, necrotic gingival tissue and bone sequestration) resulting from accidental injection of sodium hypochlorite. SUMMARY:Root canal treatment is a routine clinical procedure with few reported complications. Sodium hypochlorite (NaOCl) is commonly used as an irrigant during the p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01340.x
更新日期:2008-03-01 00:00:00