Abstract:
AIM:To investigate in vitro the cytocompatibility of the calcium silicate-containing endodontic sealers MTA Fillapex and TotalFill BC Sealer on human periodontal ligament stem cells (hPDLSCs) by assaying their biological responses and compare them with that observed when using an epoxy resin-based sealer (AH Plus). METHODOLOGY:Specimens from the three different endodontic sealers were eluated with culture medium for 24 h. The cytotoxicity of these eluates was evaluated using the MTT assay. In addition, an in vitro scratch wound healing model was used to determine their effects on cell migration. Cell adhesion to collagen type I after treatment with the different sealer eluates was also measured, whereas cytotoxicity was determined using the DNA-specific fluorochrome Hoechst 33342. Finally, to assess cell morphology and attachment to the different sealers, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). One-way analysis of variance (anova) followed by a Bonferroni post-test were performed (P < 0.05). RESULTS:hPDLSCs exposed to different dilutions of TotalFill BC Sealer eluates had significantly higher cell proliferation compared with that observed when cells were treated with AH Plus and MTA Fillapex eluates (P < 0.001). In addition, TotalFill eluates were associated with significantly increased cell adhesion to collagen type I and migration of hPDLSCs in a concentration-dependent manner than displayed after treatment with MTA Fillapex or AH Plus eluates (P < 0.001). Moreover, TotalFill BC Sealer-induced cytotoxicity was significantly lower than observed using AH Plus and MTA Fillapex eluates (P < 0.001). Finally, SEM studies revealed suitable proliferation, cell spreading and attachment, especially when using TotalFill BC Sealer discs. CONCLUSION:TotalFill BC Sealer exhibited a higher cytocompatibility than AH Plus and MTA Fillapex. Further investigations using in vivo animal models are required to validate the potential biological responses of TotalFill BC Sealer on hPDLSCs.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Rodríguez-Lozano FJ,García-Bernal D,Oñate-Sánchez RE,Ortolani-Seltenerich PS,Forner L,Moraleda JMdoi
10.1111/iej.12596subject
Has Abstractpub_date
2017-01-01 00:00:00pages
67-76issue
1eissn
0143-2885issn
1365-2591journal_volume
50pub_type
杂志文章abstract:AIM:To evaluate the effect of a calcium aluminate-based cement (CAC+) on the development of the osteogenic phenotype in vitro. METHODOLOGY:Rat calvaria-derived cells were grown on Thermanox® coverslips for 24 h and then exposed to either samples (4-h set) of CAC+ or mineral trioxide aggregate (MTA) placed on Transwell...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12682
更新日期:2017-08-01 00:00:00
abstract:AIM:To compare the fracture resistance of roots filled with different materials. METHODOLOGY:Thirty-four freshly extracted human maxillary central incisor teeth with similar dimension were selected. Crowns were sectioned at the cemento-enamel junction, and the length of the roots were adjusted to 13 mm. Following the ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01177.x
更新日期:2007-01-01 00:00:00
abstract:AIM:To detect and quantify dentine sialoprotein (DSP) in the gingival crevicular fluid (GCF) of luxated teeth. METHODOLOGY:Eighteen subjects were enroled and distributed as follows. Group I (n = 6, positive control): subjects with primary second molar teeth undergoing physiological root resorption. Group II (n = 6, ne...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12001
更新日期:2013-04-01 00:00:00
abstract:AIM:To investigate the smallest dentine thickness in mesial canals of mandibular molars along the cervical and middle thirds of the root by means of a micro-computed tomographic (micro-CT) technology and digital image analysis. METHODOLOGY:Fifty mesial roots of mandibular molars having two independent canals (mesiobuc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13141
更新日期:2019-10-01 00:00:00
abstract::The use of cone beam computed tomography (CBCT) in the diagnosis and/or management of endodontic problems is increasing and is reflected in the exponential rise in publications on this topic in the last two decades. The aim of this paper is to: (i) Review current literature on the endodontic applications of CBCT; (ii)...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13115
更新日期:2019-08-01 00:00:00
abstract:AIM:The present randomized, controlled prospective study evaluated the histomorphological response of human dental pulps capped with two grey mineral trioxide aggregate (MTA) compounds. METHODOLOGY:Pulp exposures were performed on the occlusal floor of 40 human permanent pre-molars. The pulp was capped either with Pro...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2008.01485.x
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND:Stem/progenitor cell-mediated pulpal regeneration could represent a promising therapeutic alternative in the field of clinical endodontics. AIM:The present study aimed to systematically assess and meta-analyse dental pulpal tissue regeneration, pulpal vitality and apical healing after the transplantation of...
journal_title:International endodontic journal
pub_type: 杂志文章,meta分析
doi:10.1111/iej.13177
更新日期:2019-11-01 00:00:00
abstract:AIMS:To evaluate the microbiota of endodontic infections in deciduous teeth by Checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA). METHODOLOGY:Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01805.x
更新日期:2011-03-01 00:00:00
abstract::The apical seal produced by low-temperature injection of gutta-percha, with or without root canal sealer, in the presence or absence of the smear layer was evaluated quantitatively using an electrochemical technique. The root canals of 44 teeth were prepared with the smear layer intact following a final flush of NaOCl...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00236.x
更新日期:1994-03-01 00:00:00
abstract:AIM:To examine the occurrence of Candida spp. in refractory periapical granulomas. METHODOLOGY:One hundred and three surgically removed periapical granulomas were subjected to molecular analysis for the occurrence of Candida albicans. DNA was extracted from the samples using a modified phenol/chloroform/isoamyl alcoho...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00707.x
更新日期:2003-09-01 00:00:00
abstract::In an effort to simulate the infected dental root canal, bacteria were enclosed in polyethylene tubes that were subcutaneously implanted into rats. Four different bacterial combinations, selected from nine oral species, were tested. The contents of 144 inoculated and implanted 'root canals' were analysed after 2 and 4...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1989.tb00931.x
更新日期:1989-11-01 00:00:00
abstract:AIM:To determine the influence of various irrigating solutions on the accuracy of the electronic apex locator facility in the Tri Auto ZX handpiece. METHODOLOGY:One hundred and forty teeth with single canals and mature apices, scheduled for extraction for either periodontal or prosthetic reasons in 76 patients were us...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2007.01234.x
更新日期:2007-05-01 00:00:00
abstract:AIM:To elucidate mechanisms by which mineral trioxide aggregate (MTA) suppresses pro-inflammatory cytokine mRNA expression in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. METHODOLOGY:Mineral trioxide aggregate extracts were prepared by immersing set ProRoot MTA in culture medium. RAW264.7 cells were cultu...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13386
更新日期:2020-12-01 00:00:00
abstract:AIM:To compare root canal treatments performed before and after education in a nickel-titanium rotary technique (NiTiR) with respect to costs for instrumentation and number of instrumentation sessions in a County Public Dental Service in Sweden. METHODOLOGY: Following education, 77% of the general dental practitioner...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2012.02019.x
更新日期:2012-07-01 00:00:00
abstract:AIM:To assess and compare the mechanical properties of TRUShape (TRS) with several nickel-titanium rotary instruments. METHODOLOGY:Cyclic fatigue, torsional resistance, flexibility and surface microhardness of TRS (size 25, 0.06v taper), ProTaper Next X2 (PTN X2, size 25, 0.06 taper), ProTaper Gold (PTG F2; size 25, 0...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12700
更新日期:2017-08-01 00:00:00
abstract:AIM:To identify, search for and critically interpret the results from clinical studies on single- versus multiple-visit root canal treatment in the light of an evidence-based paradigm. For that purpose, the quality of the available body of evidence was assessed using the SORT 'grade' - Strength of Recommendation Taxono...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12621
更新日期:2017-03-01 00:00:00
abstract:AIM:To assess the failure mechanism of rotary NiTi instruments by chemical, structural and morphological analyses to provide a rational explanation of the effects of surface and bulk treatments on their resistance to fatigue fracture. METHODOLOGY:Thermal treatment (350-500 °C) was performed on electropolished (EP) and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01759.x
更新日期:2010-10-01 00:00:00
abstract:AIM:This randomized, double-blind, placebo-controlled, clinical trial was designed to improve the success of inferior alveolar nerve blocks (IANB) in mandibular molars with symptomatic irreversible pulpitis (SIP) by means of preoperative submucosal administration of 50 mg tramadol. METHODOLOGY:Forty-two patients with ...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/iej.12755
更新日期:2017-12-01 00:00:00
abstract:AIM:To develop and preliminarily evaluate a new screening instrument for atypical odontalgia (AO) or persistent dentoalveolar pain disorder (PDAP). To evaluate the instrument's performance in detecting AO/PDAP amongst a heterogeneous group of orofacial pain conditions and pain-free controls and empirically compare its ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13017
更新日期:2019-03-01 00:00:00
abstract:AIM:To evaluate, using an experimental immature tooth model, the fracture resistance of bovine incisors submitted to different reinforcement treatments with mineral trioxide aggregate (MTA). METHODOLOGY:An immature tooth model was created by sectioning the coronal and apical portions of 40 bovine incisors 8 mm above a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01266.x
更新日期:2007-09-01 00:00:00
abstract::The cutting heads of the first 18 instruments in six sets of clinically used Lightspeed instruments (sizes 20-65) were examined in a scanning electron microscope (SEM). Three sets had been used by full-time faculty and discarded as follows: instrument sizes 20-32.5 after 18 treated root canals; instrument sizes 35-47....
journal_title:International endodontic journal
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:AIM:To investigate the effects of vascular endothelial growth factor A (VEGFA) and the underlying molecular mechanisms on the migration of human dental pulp stem cells (hDPSCs). METHODOLOGY:The expression of VEGFA in inflammatory pulp tissue and lipopolysaccharide (LPS)-stimulated dental pulp cells was examined by imm...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13179
更新日期:2019-12-01 00:00:00
abstract:AIM:To present a scientometric analysis of the entire body of scientific publications in the field of vital pulp therapy (VPT) and analyse the research trends and popular topics. METHODOLOGY:A comprehensive literature search was conducted in the Web of Science and Scopus databases on 21 August 2020 to identify all art...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13422
更新日期:2021-02-01 00:00:00
abstract:AIM:To evaluate the efficacy of a prototype root canal dressing containing surface pre-reacted glass-ionomer (S-PRG) fillers on repairing induced periapical lesions in a rat model. Calcium hydroxide [Ca(OH)2 ] was applied as a comparison in the healing process. METHODOLOGY:The pulp chambers of the maxillary first mola...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13414
更新日期:2021-02-01 00:00:00
abstract:AIM:To compare fracture patterns of posterior teeth temporized with: mesio-occlusal-distal (MOD) glass-ionomer cement (GIC) interim restorations, stainless steel (SS) bands, GIC dome overlays and GIC dome overlays with occlusal temporary restorations. METHODOLOGY:The root canals of 92 extracted human premolars and mol...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12102
更新日期:2013-12-01 00:00:00
abstract:AIM:To report the hydration mechanism of white mineral trioxide aggregate (White MTA, Dentsply, Tulsa Dental Products, Tulsa, OK, USA). METHODOLOGY:The chemical constitution of white MTA was studied by viewing the powder in polished sections under the scanning electron microscope (SEM). The hydration of both white MTA...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01248.x
更新日期:2007-06-01 00:00:00
abstract::The typical treatment for irreversibly inflamed/necrotic pulp tissue is root canal treatment. As an alternative approach, regenerative endodontics aims to regenerate dental pulp-like tissues using two possible strategies: cell transplantation and cell homing. The former requires exogenously transplanted stem cells, co...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12868
更新日期:2018-04-01 00:00:00
abstract:AIM:To determine whether nisin, a bacteriocin, would be effective at killing Enterococcus faecalis and Streptococcus gordonii cells in solution and within the root canal system. METHODOLOGY:Bacterial isolates of E. faecalis and S. gordonii were grown from glycerol stocks in closed tubes containing BHY broth at 37 degr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00846.x
更新日期:2004-10-01 00:00:00
abstract::The purpose of this study was to observe the quality of seal of the glass ionomer cement, Ketac-Endo, after treatment of the root canal wall. The root canals of 140 extracted human teeth were prepared biomechanically. The root canals were treated with either EDTA or received an intracanal dressing of calcium hydroxide...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1995.tb00298.x
更新日期:1995-07-01 00:00:00
abstract:AIM:Apatite-forming ability, considered as an index of bioactivity (bond-to-bone ability), was tested on ProRoot MTA cement after immersion in phosphate-containing solution (DPBS). METHODOLOGY:Disk samples were prepared and immersed in DPBS for 10 min, 5 h, 1 and 7 days. The cement surface was studied by attenuated to...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01768.x
更新日期:2010-10-01 00:00:00