Abstract:
AIM:To examine the occurrence of Candida spp. in refractory periapical granulomas. METHODOLOGY:One hundred and three surgically removed periapical granulomas were subjected to molecular analysis for the occurrence of Candida albicans. DNA was extracted from the samples using a modified phenol/chloroform/isoamyl alcohol method and was subjected to polymerase chain reaction (PCR) with OPA-03 and repetitive sequence (GACA)4 primers. The PCR products were separated in agarose gel electrophoresis, stained with ethidium bromide, visualized using UV light and the sequences were analysed. Samples indicating possible occurrence of Candida were further investigated by histological and immunohistological methods. Periodic acid-Shiff staining (PAS) was used to detect yeast cells and hyphae, and specific monoclonal antibodies to recognize high molecular mass mannoproteins present in the C. albicans cell wall. DNA extraction was controlled by running PCR using beta-actin primers (a housekeeping gene). C. albicans CCUG19915, C. tropicalis ATCC750, C. krusei ATCC6258, C. guilliermondii ATCC6260 and C. glabrata CCUG32725 served as positive controls in PCR. A tissue preparation of chronic atrophic candidosis in oral buccal mucosa served as a positive control for histological and immunohistological examinations. RESULTS:Polymerase chain reaction with beta-actin primers indicated successful DNA extraction in 68 out of 103 samples. The majority of the samples (50) were negative whereas 18 of the samples showed PCR products indicating possible occurrence of Candida spp. PAS-staining and immunohistological examination of these samples were, however, negative. Further analysis of the PCR products revealed sequences not typical for Candida spp. CONCLUSIONS:Candida spp. do not seem to occur in periapical granuloma.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Waltimo T,Kuusinen M,Järvensivu A,Nyberg P,Väänänen A,Richardson M,Salo T,Tjäderhane Ldoi
10.1046/j.1365-2591.2003.00707.xkeywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
643-7issue
9eissn
0143-2885issn
1365-2591pii
707journal_volume
36pub_type
杂志文章abstract:AIM:To evaluate the frequency of dentinal microcracks after ultrasonic removal of fractured files from the middle third of root canals using micro-computed tomography (micro-CT). METHODOLOGY:Eighteen bilaterally matched pairs of human mandibular incisors extracted for periodontal reasons were included. The matched pai...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12909
更新日期:2018-09-01 00:00:00
abstract::Dentoalveolar ankylosis is a common complication after replantation of teeth with injured periodontal ligaments. This is a serious complication for the tooth since it leads to progressive root resorption. In young individuals it may also cause infra-occlusion and reduced development of the alveolar process. The purpos...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00549.x
更新日期:1993-03-01 00:00:00
abstract:AIM:To report a case of reparative bone-like tissue formation in the tooth of a patient with systemic sclerosis. SUMMARY:A 58-year-old Japanese female patient with systemic sclerosis was referred because of tooth fracture. Cone beam computerized tomography (CBCT) revealed multiple root resorption and the unclear trans...
journal_title:International endodontic journal
pub_type:
doi:10.1111/iej.12914
更新日期:2018-09-01 00:00:00
abstract:AIM:To assess the free available chlorine concentration (FAC), organic tissue dissolution, and smear layer removal capacity of sodium hypochlorite (NaOCl) alone and when mixtured with etidronate (HEDP) and tetrasodium EDTA (Na4 EDTA), and heated to different temperatures. METHODOLOGY:Mixtures at 1:1 ratio of 5% NaOCl ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13450
更新日期:2020-11-20 00:00:00
abstract:AIM:To investigate whether apical periodontitis lesions infected by Epstein-Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8-hydroxydeoxyguanosine (8-OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF-κB) ligand (RANKL) and osteoproteg...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12886
更新日期:2018-06-01 00:00:00
abstract:AIM:To investigate the reliability of using bovine teeth as an alternative to human teeth in an intra-tooth push-out model by comparing the bond strength of three root canal sealers in both tooth substrates. METHODOLOGY:From human and bovine incisors (12 per group), one 1-mm disc was obtained from each root. On each d...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13085
更新日期:2019-07-01 00:00:00
abstract:AIM:To determine whether the outcome of cone beam computed tomography (CBCT) examinations performed in accordance with the European Commission guidelines in a clinical setting has an impact on choosing diagnoses in endodontics. METHODOLOGY:A prospective observational study was conducted. Fifty-three consecutive patien...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12350
更新日期:2015-06-01 00:00:00
abstract:AIM:To analyse responses from dental practitioners (DPs) on how secure they felt as a newly graduated dentist, level of confidence or self-efficacy when performing root canal treatment (RCT), and if undergraduate (UG) education in Endodontics adequately met their needs in a dental practice. METHODOLOGY:An electronic q...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13455
更新日期:2020-11-30 00:00:00
abstract:AIM:To investigate amongst Greek endodontists the prevalence and characteristics of musculoskeletal disorders (MSDs) in the past 12 months, the treatment followed, their postures during practice and the adoption of ergonomic standards. METHODOLOGY:One hundred and forty-seven endodontists met the inclusion criteria and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12219
更新日期:2014-08-01 00:00:00
abstract:AIM:To evaluate radiopacity of root filling materials using digital radiography. METHODOLOGY:The sealers tested were AH Plus, Endofill, EndoREZ and Epiphany. Gutta-percha (Dentsply Maillefer) and Resilon cones were also tested. Acrylic plates, containing six wells, measuring 1 mm in depth and 5 mm in diameter, were pr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01246.x
更新日期:2007-07-01 00:00:00
abstract::The aim of this report is to (i) review the current literature on the status of root filled teeth, (ii) analyse the most important factors in decision-making, (iii) discuss the current restorative concepts, and (iv) classify both the evidence and clinical practice in a way that seeks to be clear, understandable and he...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12847
更新日期:2018-03-01 00:00:00
abstract:AIM:The aim of this in vitro study was to evaluate the antimicrobial effectiveness of electro-chemically activated water (ECA) as an endodontic irrigation solution. METHODOLOGY:The root canals of 60 caries-free, single-rooted, adult, maxillary, anterior human teeth were instrumented and irrigated in a similar method t...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2001.00258.x
更新日期:2001-04-01 00:00:00
abstract:AIM:To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. METHODOLOGY:A total of 50 human periodontal ligament samples were obtained from health...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12952
更新日期:2018-12-01 00:00:00
abstract:AIM:To describe the use of a new protective device to reduce aerosol dispersion in dental clinics during the COVID-19 pandemic. METHODOLOGY:The device consists of a rigid translucent acrylic structure (methyl polymethacrylate), adjusted to the dental chair, involving the patient's head, neck and chest. There is also a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13373
更新日期:2020-11-01 00:00:00
abstract:AIM:To develop a precise and simple mounting model (MM) for evaluating apex locators and to compare the repeatability of the MM with a conventional visual method (VM). METHODOLOGY:Electronic working length determination was performed in 32 maxillary central incisors using two methods: (i) the MM method and (ii) a conv...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01052.x
更新日期:2006-02-01 00:00:00
abstract:AIM:To compare several parameters of root canal preparation using two different rotary nickel-titanium (Ni-Ti) instruments: FlexMaster (VDW, Munich, Germany) and HERO 642 (Micro-Mega, Besançon, France). METHODOLOGY:Fifty extracted human mandibular molars with root canal curvatures between 20 and 40 degrees were embedd...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00664.x
更新日期:2003-05-01 00:00:00
abstract::In this study, an unusual observation of Saccharomyces cerevisiae isolated from an infected root canal and from the blood of a patient undergoing endodontic therapy of a tooth with asymptomatic apical periodontitis is reported. Phenotypic (biochemical tests, antifungal susceptibility tests and SDS-PAGE of cellular pro...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00101.x
更新日期:1997-09-01 00:00:00
abstract:AIM:To assess the effectiveness of an active application of liquid etching, compared with the standard gel formulation on smear layer removal from post space walls and push-out bond strength of luted fibre posts. METHODOLOGY:Human extracted teeth were collected and root filled. After post space preparation and cleanin...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12096
更新日期:2013-11-01 00:00:00
abstract:AIM:To evaluate the presence of bacteria in permanent teeth with intact crowns (without caries, periodontal disease or dental trauma) in patients with sickle cell anaemia (HbSS genotype) by analysing their clinical, imaging and microbiological parameters. METHODOLOGY:This is a case series study nested in a cohort. In ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13476
更新日期:2021-01-12 00:00:00
abstract::A rare case of dens invaginatus involving a non-carious mandibular lateral incisor with periapical periodontitis is reported. Invaginated teeth present technical difficulties in their clinical management because of their abnormal anatomy. In the present case, non-surgical root canal therapy was chosen. A one-year foll...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1995.tb00320.x
更新日期:1995-11-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12658
更新日期:2017-06-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01681.x
更新日期:2010-04-01 00:00:00
abstract:AIM:To report a case of focal cemento-osseous dysplasia (FCOD) affecting a single tooth misdiagnosed as an inflammatory periapical lesion. SUMMARY:The patient, a black 47-year-old woman complained of pain affecting the right side of the mandible. Routine X-ray examination discovered a periapical radiolucency on the ma...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2003.00736.x
更新日期:2003-12-01 00:00:00
abstract:AIM:The aim of this study was to determine the influence of the width of apical root canals and the depth of heat application during warm vertical compaction on the percentage of gutta-percha-filled area (PGFA) in the apical root canal. METHODOLOGY:Two groups of extracted human canines (total 60) were instrumented and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00522.x
更新日期:2002-06-01 00:00:00
abstract:AIM:To compare the area occupied by gutta-percha, sealer, or void in standardized .06 tapered prepared simulated curved canals and in mesio-buccal canals of extracted maxillary first molars filled with a single .06 gutta-percha point and sealer or lateral condensation of multiple .02 gutta-percha points and sealer. ME...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00903.x
更新日期:2005-02-01 00:00:00
abstract:AIM:To investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. METHODOLOGY:The study included 16 healthy and 16 T2D participants who underwent vital pulp ex...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12934
更新日期:2018-10-01 00:00:00
abstract:AIM:To assess the inflammatory potential of biofilm matrix constituents of Enterococcus faecalis and Pseudomonas aeruginosa monospecies biofilms on macrophages. METHODOLOGY:In vitro biofilms of E. faecalis and P. aeruginosa were grown (7 days) in aerobic and anaerobic conditions. The biofilm matrix components: exopoly...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13093
更新日期:2019-07-01 00:00:00
abstract:AIM:To evaluate the percentage of gutta-percha and sealer-filled canal area when four different types of master gutta-percha cones were used. METHODOLOGY:Sixty mandibular premolars with straight canals were instrumented using System ProTaper rotary instruments with a crown-down technique and assigned to four groups. C...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01533.x
更新日期:2009-06-01 00:00:00
abstract:LITERATURE REVIEW:Inter-appointment flare-up is characterized by the development of pain, swelling or both, following endodontic intervention. The causative factors of flare-ups encompass mechanical, chemical and/or microbial injury to the pulp or periradicular tissues. Of these factors, microorganisms are arguably the...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00671.x
更新日期:2003-07-01 00:00:00
abstract:AIM:To evaluate ex vivo the long-term sealing ability of the SE Resilon Epiphany system as an apical root-end filling material. METHODOLOGY:A total of 60 standardized horizontal bovine root sections were divided into three groups filled with either gutta-percha with AH 26, tooth-coloured mineral trioxide aggregate (MT...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01583.x
更新日期:2009-10-01 00:00:00