Abstract:
AIM:To evaluate the presence of bacteria in permanent teeth with intact crowns (without caries, periodontal disease or dental trauma) in patients with sickle cell anaemia (HbSS genotype) by analysing their clinical, imaging and microbiological parameters. METHODOLOGY:This is a case series study nested in a cohort. In the first follow-up of this cohort study (Journal of Endodontics, 2013, 39, 177), 10 HbSS patients with at least one tooth with an intact crown and clinically diagnosed with pulp necrosis by pulse oximetry adapted for dentistry and a cold pulp sensitivity test (n = 27 teeth) were selected. Changes in the pulp chamber, root and periodontal ligament were identified in the tomographic analysis. Bacterial culture, staining for live and dead bacteria, and real-time polymerase chain reaction with 16S rRNA primers were used to identify the presence of bacteria. Culture sample collection was performed immediately after access to the pulp chamber. The microbiome was analysed with a MiSeq sequencer (Illumina, San Diego, CA). RESULTS:The diagnosis of pulp necrosis was confirmed clinically in 82% (22/27) of the teeth. The amount of bacterial load identified was less than 100 copies μL-1 in 23% (5/22) of the teeth with intact crowns and pulp necrosis. Thirteen bacterial species were identified that are commonly found in urinary tract infections, septicaemia and infective endocarditis. Only one of these species, Granulicatella adjacens, has also be found in primary endodontic infections. CONCLUSION:Prospective clinical, imaging and microbiological analyses suggest that pulp necrosis of teeth with intact crowns in HbSS patients is not associated with the presence of bacteria.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Costa CPS,Silva MA,Neto LGL,Valois EM,Monteiro-Neto V,Souza SFCdoi
10.1111/iej.13476subject
Has Abstractpub_date
2021-01-12 00:00:00eissn
0143-2885issn
1365-2591pub_type
杂志文章abstract:AIM:To compare the cyclic fatigue resistance of ProTaper Next files (PTN; Dentsply Maillefer, Ballaigues, Switzerland) with Twisted Files (TF; SybronEndo, Orange, CA, USA), HyFlex CM (HF; ColténeEndo/Whaledent, Inc, Cuyahoga Falls, OH, USA) and ProTaper Universal (PT; Dentsply Maillefer). METHODOLOGY:Size 25, .06 tape...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12244
更新日期:2014-11-01 00:00:00
abstract:AIM:To investigate the root and canal morphology of mandibular permanent teeth collected from an indigenous Turkish population. METHODOLOGY:A total of 1400 extracted mandibular permanent teeth were evaluated. The teeth were divided into seven groups as central incisors, lateral incisors, canines, first premolars, seco...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00837.x
更新日期:2004-07-01 00:00:00
abstract:AIM:To identify the role of astrocyte elevated gene-1 (AEG-1) and the mechanism underlying the inflammatory response in dental pulp cells. METHODOLOGY:Astrocyte elevated gene-1 expression was detected at different stages of pulpitis in a rat model using immunohistochemistry. RT-qPCR and Western blot were used to asses...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12921
更新日期:2018-10-01 00:00:00
abstract:AIM:To provide insight into the principles of operation of electronic apex locators, determine optimal measuring parameters of the impedance ratio method, and to evaluate its accuracy. METHODOLOGY:Electrical impedance was measured ex vivo on 14 extracted human teeth using a QuadTech 1920 precision impedance analyzer. ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01589.x
更新日期:2009-09-01 00:00:00
abstract:AIM:To evaluate ex vivo the quality of root fillings completed by two thermoplasticized gutta-percha techniques (Thermafil and System B) and a cold gutta-percha technique (single point) by μCT analysis. METHODOLOGY:A total of 30 freshly extracted human single-rooted permanent teeth were selected. Root canals were prep...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01840.x
更新日期:2011-04-01 00:00:00
abstract::This European Society of Endodontology (ESE) position statement on surgical extrusion, intentional replantation and tooth autotransplantation represents the consensus of an expert committee, convened by the ESE. A narrative review in the International Endodontic Journal (Plotino et al. 2020, international Endodontic J...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13456
更新日期:2021-01-26 00:00:00
abstract::The aim of this study was to evaluate the accuracy of the electronic apex locator Root ZX compared to the Sono Explorer Mark II Junior and Apit III in detecting root perforations. Extracted human teeth (n = 30) were perforated artificially in the middle third of the root and embedded in a box containing alginate. Dete...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00107.x
更新日期:1997-11-01 00:00:00
abstract:AIM:To investigate whether apical periodontitis lesions infected by Epstein-Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8-hydroxydeoxyguanosine (8-OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF-κB) ligand (RANKL) and osteoproteg...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12886
更新日期:2018-06-01 00:00:00
abstract:AIM:To assess the effect of acid-etching duration time on the compressive strength, microstructure and shear bond strength of All-Bond Universal adhesive to MTA-Angelus (AMTA), and Biodentine (BD). METHODOLOGY:Forty-eight cylindrical specimens (4 mm in diameter, 6 mm in height) of AMTA in four groups and 48 specimens ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13026
更新日期:2019-04-01 00:00:00
abstract::CLINICAL TECHNIQUE: It is generally accepted that the success of root-canal treatment is mainly dependent on the proper cleaning, shaping and hermetic obturation of root canals. Obturation of the root canal is of importance as 60% of endodontic failures are attributable to incomplete obliteration of the root-canal sys...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00180.x
更新日期:1999-01-01 00:00:00
abstract:AIM:To investigate the cytotoxicity of a modified salt solution (MSS) and evaluate the antimicrobial properties of MSS on in vitro biofilm models. METHODOLOGY:In a metabolic assay, fibroblasts derived from periodontal ligaments (PDL) of human extracted teeth were cultured and challenged with MSS or controls. Then, in ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12294
更新日期:2015-02-01 00:00:00
abstract::Angiogenesis is the formation of new blood vessels based on a pre-existing vasculature. It comprises two processes, sprouting of endothelial cells and the division of vessels due to abnormal growth of the microvasculature. It has been demonstrated that substance P (SP) can induce angiogenesis either by modulating endo...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12627
更新日期:2017-04-01 00:00:00
abstract:AIM:To investigate the prevalence of periradicular periodontitis (PRP) using cone-beam computed tomography (CBCT) scans in a retrospective cross-sectional epidemiological study in a Scottish subpopulation. METHODOLOGY:Of the 319 CBCT scans performed at Dundee Dental Hospital between November 2009 and July 2012, 245 de...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12228
更新日期:2014-09-01 00:00:00
abstract:AIMS:(i) To compare the prevalence of extrusion of root filling material when placed using different root filling techniques, with or without customization of the master gutta-percha (GP) cone; and (ii) to investigate the effects of some factors influencing root filling extrusion and presence of voids. METHODOLOGY:A t...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.01005.x
更新日期:2005-09-01 00:00:00
abstract:AIM:To evaluate the bond strength of a resin-based sealer (AH Plus) to root canal dentine after the removal of a zinc oxide-eugenol-based sealer (Endofill), using different retreatment techniques. METHODOLOGY:The root canals of sixty-four maxillary incisors were prepared with ProTaper and filled with Endofill/gutta-pe...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12170
更新日期:2014-05-01 00:00:00
abstract::The effect on coronal leakage of the use of either a single gutta-percha cone or cold lateral condensation of gutta-percha, with a glass ionomer sealer cement was studied. In addition, the effect of the removal of the smear layer was also determined. Eighty-eight single-rooted teeth were prepared chemomechanically usi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00255.x
更新日期:1994-07-01 00:00:00
abstract:AIM:To determine if a final rinse with EDTA improves the short-term in-tooth retentive strength of adhesively luted fibre-reinforced composite posts. METHODOLOGY:Six different commercial fibre post systems were tested. Post spaces in extracted human anterior teeth were irrigated with either 6% NaOCl or 6% NaOCl follow...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01482.x
更新日期:2008-12-01 00:00:00
abstract::An in-vitro investigation was performed to assess the extent of apical microleakage in simulated canals in clear resin blocks. Four different methods of producing dye penetration were employed: passive dye penetration alone, centrifugation alone, vacuum plus passive dye penetration, and increased pressure plus passive...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1996.tb01362.x
更新日期:1996-05-01 00:00:00
abstract:AIM:The purpose of this study was to investigate the expression of cyclooxygenase-2 (COX-2) in radicular cysts. METHODOLOGY:Thirty biopsy specimens of radicular cysts were examined using immunohistochemistry. A peroxidase-labelled streptavidin-biotin technique was used for identification of the COX-2. Fisher's exact t...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00584.x
更新日期:2002-10-01 00:00:00
abstract:AIM:To use micro-computed tomography (μ-CT) to evaluate the amount of calcium hydroxide [Ca(OH)2 ] remaining in the C-shaped root canals of mandibular second molars after attempting to remove it with passive ultrasonic and sonic irrigation. METHODOLOGY:Thirty mandibular second molars, 15 in C1 and 15 in C2 configurati...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12319
更新日期:2015-04-01 00:00:00
abstract:AIM:To evaluate ex vivo the incidence of defects in root dentine before and after root canal preparation and filling. METHODOLOGY:Eighty extracted mandibular premolars were divided equally in four groups. Group 1 was left unprepared. All other root canals were prepared using Gates Glidden drills and System GT files up...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01502.x
更新日期:2009-03-01 00:00:00
abstract:AIM:To investigate the influence of biofilm structure on the biofilm removal capacity of endodontic irrigants and to study changes in the architecture of the remaining biofilms. METHODOLOGY:Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were cocultured under different growth conditions on saliva-coated hy...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13027
更新日期:2019-04-01 00:00:00
abstract:AIMS:To identify Gram-positive rods from root canals of teeth with apical periodontitis and to examine their associations with other species. METHODOLOGY:Consecutive root canal samples (RCSs) from 139 teeth undergoing root canal treatment were analyzed prospectively for cultivable microbes. Gram-positive rods in the f...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00845.x
更新日期:2004-09-01 00:00:00
abstract::A light-cured ionomer cement was investigated as a retrograde root seal, without a retrograde cavity. This was compared with the material used in a retrograde cavity, and with a conventional glass ionomer cement, as a seal. The adaptation and sealing ability of the test materials were assessed using a confocal optical...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00562.x
更新日期:1993-07-01 00:00:00
abstract:AIM:To evaluate the effect of rotational speed and the angle and radius of curvature of root canals on the fracture of two types of nickel-titanium rotary instruments: K3 and ProTaper. METHODOLOGY:A total of 240 root canals of extracted human maxillary and mandibular molars were divided into two groups of 120, accordi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00630.x
更新日期:2003-04-01 00:00:00
abstract:AIM:To evaluate the performance of XP-endo Finisher R instruments when removing root filling remnants from oval-shaped canals using microcomputed tomographic (micro-CT) imaging as the analytical tool. Passive ultrasonic irrigation (PUI) was used as a reference technique for comparison. METHODOLOGY:Twenty mandibular in...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13077
更新日期:2019-06-01 00:00:00
abstract:AIM:To evaluate the torsional properties of pathfinding nickel-titanium (NiTi) rotary instruments manufactured from several NiTi alloys, ProGlider (M-wire), Hyflex GPF (conventional NiTi Wire and controlled memory wire), Logic (conventional NiTi wire and controlled memory wire) and Mtwo (conventional NiTi wire). METHO...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12879
更新日期:2018-06-01 00:00:00
abstract:AIM:This randomized, double-blinded, clinical trial evaluated the effect of oral premedication of piroxicam, prednisolone, dexamethasone or placebo on postoperative pain after single-visit root canal treatment in teeth with symptomatic irreversible pulpitis and symptomatic apical periodontitis. METHODOLOGY:The trial i...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/iej.13416
更新日期:2021-02-01 00:00:00
abstract:AIM:To evaluate the accuracy of the Root ZX and Elements-Diagnostic electronic apex locators when compared with radiographs for locating the canal terminus or minor foramen. METHODOLOGY:The canal terminus of 482 canals in 160 maxillary and mandibular teeth was located in vivo with both locators and radiographically. A...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01620.x
更新日期:2010-01-01 00:00:00
abstract::The assurance of the quality of a service rendered by a member of the dental profession is an essential feature of any system of peer review in dentistry. This document addresses two essential elements: (i) appropriateness of treatment modality and (ii) quality or level of treatment rendered. In revising these guideli...
journal_title:International endodontic journal
pub_type: 共识发展会议,杂志文章,实务指引
doi:10.1111/j.1365-2591.2006.01180.x
更新日期:2006-12-01 00:00:00