Vascular endothelial growth factor A/Vascular endothelial growth factor receptor 2 axis promotes human dental pulp stem cell migration via the FAK/PI3K/Akt and p38 MAPK signalling pathways.

Abstract:

AIM:To investigate the effects of vascular endothelial growth factor A (VEGFA) and the underlying molecular mechanisms on the migration of human dental pulp stem cells (hDPSCs). METHODOLOGY:The expression of VEGFA in inflammatory pulp tissue and lipopolysaccharide (LPS)-stimulated dental pulp cells was examined by immunofluorescence staining and qRT-PCR. The migration of hDPSCs was detected using transwell migration and wound healing assays. The activation of FAK, PI3K, Akt and p38 signalling was evaluated by Western blot analysis. Silence RNA (siRNA) technology was utilized to knockdown the expression of VEGFR1 (Flt-1) and VEGFR2 (Flk-1/KDR). PF573228 (inhibitor of FAK), LY294002 (inhibitor of PI3K), SB203580 (inhibitor of p38) and SU5416 (inhibitor of VEGFR2) were employed to investigate the effect of VEGFA on the migratory mechanism of hDPSCs. Data were analysed statistically using the Student's t-test or one-way ANOVA. RESULTS:The expression levels of VEGFA in inflammatory pulp tissue in vivo and LPS-stimulated dental pulp cells in vitro were significantly greater than those in the control groups (P < 0.05). Vascular endothelial growth factor A promoted the migration of hDPSCs in a concentration-dependent manner. Several signalling pathways, including FAK, PI3K, Akt and p38, were activated by VEGFA in a dose- and time-dependent manner in hDPSCs. The VEGFA-induced migration of hDPSCs was significantly inhibited with drug inhibitors such as PF573228, LY294002, SB203580 or SU5416 (P < 0.05). These signalling pathways activated by VEGFA stimulation were significantly suppressed by pre-treatment with inhibitor of VEGFR2 (SU5416) or transfection with siRNA of VRGFR2 (P < 0.05) but not VEGFR1 siRNA. CONCLUSIONS:Vascular endothelial growth factor A/VEGFR2 axis promoted the migration of hDPSCs via the FAK/PI3K/Akt and p38 MAPK signalling pathways. These findings reveal a novel molecular mechanism for cell migration of hDPSCs, which may contribute to the remodelling of pulp tissue and dentine.

journal_name

Int Endod J

authors

Sun X,Meng L,Qiao W,Yang R,Gao Q,Peng Y,Bian Z

doi

10.1111/iej.13179

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

1691-1703

issue

12

eissn

0143-2885

issn

1365-2591

journal_volume

52

pub_type

杂志文章
  • Tissue necrosis after using an arsenical endodontic preparation: a case report.

    abstract::A case is reported of severe loss of alveolar bone in the maxilla, as a result of leakage into the tissues of an arsenical paste from the pulp chamber of an endodontically 'treated' tooth. The history of the use of arsenic in dentistry is discussed, as are the hazards inherent in the use of this outmoded and dangerous...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:

    authors: Smart ER,Barnes IE

    更新日期:1991-09-01 00:00:00

  • Focal cemento-osseous dysplasia involving a mandibular lateral incisor.

    abstract:AIM:To report a case of focal cemento-osseous dysplasia (FCOD) affecting a single tooth misdiagnosed as an inflammatory periapical lesion. SUMMARY:The patient, a black 47-year-old woman complained of pain affecting the right side of the mandible. Routine X-ray examination discovered a periapical radiolucency on the ma...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2003.00736.x

    authors: Galgano C,Samson J,Küffer R,Lombardi T

    更新日期:2003-12-01 00:00:00

  • Reliability of different electronic apex locators to detect root perforations in vitro.

    abstract::The aim of this study was to evaluate the accuracy of the electronic apex locator Root ZX compared to the Sono Explorer Mark II Junior and Apit III in detecting root perforations. Extracted human teeth (n = 30) were perforated artificially in the middle third of the root and embedded in a box containing alginate. Dete...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.1997.00107.x

    authors: Kaufman AY,Fuss Z,Keila S,Waxenberg S

    更新日期:1997-11-01 00:00:00

  • Proposal for a standardized protocol for the systematic orofacial examination of patients with Hereditary Sensory Radicular Neuropathy.

    abstract:AIM:To apply a standardized protocol for the orofacial evaluation of two adult siblings (one male and one female) with Hereditary Sensory Radicular Neuropathy (HSRN) that presented with dental problems. SUMMARY:The systematic evaluation consisted of (a) clinical questionnaire; (b) radiographs [orthopantomography and c...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2006.01160.x

    authors: Siqueira SR,Okada M,Lino AM,Teixeira MJ,Siqueira JT

    更新日期:2006-11-01 00:00:00

  • The design and development of surgical templates for premolar transplants in adolescents.

    abstract::The aim of designing and fabricating the surgical templates was to assist the surgical component of premolar transplantation, by establishing and replicating the root dimensions of the donor premolar tooth. The correct template could be used to assess the socket preparation (width and depth) prior to placement of the ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2012.02077.x

    authors: Day PF,Lewis BR,Spencer RJ,Barber SK,Duggal M

    更新日期:2012-11-01 00:00:00

  • Thermal sensitivity of endodontically treated teeth.

    abstract:CASE REPORTS:The problem of thermal sensitivity following non-surgical root-canal treatment is explored and case reports are presented. Possible causes for post-treatment discomfort from endodontic and restorative aetiologies are discussed, as are the mechanisms to explain the patients' painful experiences. Treatment o...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.1999.00197.x

    authors: Tidwell E,Witherspoon DE,Gutmann JL,Vreeland DL,Sweet PM

    更新日期:1999-03-01 00:00:00

  • The use of glass ionomer as a root canal sealer--a pilot study.

    abstract::The use of a resin-based glass ionomer as an endodontic sealer was studied in vitro. The root canals of extracted single-rooted human teeth with mature apices were prepared using a stepback technique. Subsequently, the smear layer was removed with 40% citric acid and the canals obturated using lateral condensation of ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1992.tb01156.x

    authors: Saunders WP,Saunders EM,Herd D,Stephens E

    更新日期:1992-09-01 00:00:00

  • Radiographic evaluation of cases referred for surgical endodontics.

    abstract:AIM:The aim of this study is to test the hypothesis that more patients with failed root-canal treatment or other endodontic problems are referred for periradicular surgery rather than nonsurgical re-treatment. METHODOLOGY:Three sets of 100 periapical radiographs representing typical cases referred for surgical treatme...

    journal_title:International endodontic journal

    pub_type: 杂志文章,多中心研究

    doi:10.1046/j.1365-2591.2002.00510.x

    authors: Doornbusch H,Broersma L,Boering G,Wesselink PR

    更新日期:2002-05-01 00:00:00

  • Accidental swallowing of an endodontic file.

    abstract:AIM:To report the management of an iatrogenic incident in which an endodontic file was swallowed accidentally and passed into the gastrointestinal tract. SUMMARY:A 51-year-old male swallowed a 21 mm, size 30 endodontic file during root canal treatment without rubber dam. In the absence of clinical complications, the p...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2008.01392.x

    authors: Kuo SC,Chen YL

    更新日期:2008-07-01 00:00:00

  • Comparison of working length determination with radiographs and two electronic apex locators.

    abstract:AIM:To evaluate the accuracy of the Root ZX and Elements-Diagnostic electronic apex locators when compared with radiographs for locating the canal terminus or minor foramen. METHODOLOGY:The canal terminus of 482 canals in 160 maxillary and mandibular teeth was located in vivo with both locators and radiographically. A...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2009.01620.x

    authors: Vieyra JP,Acosta J,Mondaca JM

    更新日期:2010-01-01 00:00:00

  • The influence of irrigating solutions on the accuracy of the electronic apex locator facility in the Tri Auto ZX handpiece.

    abstract:AIM:To determine the influence of various irrigating solutions on the accuracy of the electronic apex locator facility in the Tri Auto ZX handpiece. METHODOLOGY:One hundred and forty teeth with single canals and mature apices, scheduled for extraction for either periodontal or prosthetic reasons in 76 patients were us...

    journal_title:International endodontic journal

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1365-2591.2007.01234.x

    authors: Erdemir A,Eldeniz AU,Ari H,Belli S,Esener T

    更新日期:2007-05-01 00:00:00

  • An in vitro comparison of adhesive systems to seal pulp chamber walls.

    abstract:AIM:To compare in vitro the sealing properties of five different dentine adhesive materials (Prime&Bond NT (PBNT); Prompt L-Pop (PLP); Clearfil SE Bond (CSEB); Scotchbond Multi Purpose Plus (SMPP); EBS-Multi (EBSM)) inside the pulp chamber. METHODOLOGY:Seventy-five recently extracted human molar teeth were used. The r...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.0143-2885.2004.00790.x

    authors: Ozturk B,Ozer F,Belli S

    更新日期:2004-05-01 00:00:00

  • Canal and isthmus debridement efficacies of two irrigant agitation techniques in a closed system.

    abstract:AIM:To compare canal and isthmus debris debridement efficacies of the manual dynamic irrigation (MDI) and apical negative pressure (ANP) techniques in the mesial root of mandibular first molars with narrow isthmi, using a closed canal design. METHODOLOGY:Micro-computed tomography was employed to select 20 teeth, each ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2010.01778.x

    authors: Susin L,Liu Y,Yoon JC,Parente JM,Loushine RJ,Ricucci D,Bryan T,Weller RN,Pashley DH,Tay FR

    更新日期:2010-12-01 00:00:00

  • Prescribing endodontic retreatment: towards a theory of dentist behaviour.

    abstract::The large variations observed in dentists' management of periapical lesions in endodontically treated teeth suggest that disease concepts used in clinical practice should be investigated. In the present study it was hypothesized that dentists regard various periapical conditions as different stages on a health continu...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1994.tb00270.x

    authors: Kvist T,Reit C,Esposito M,Mileman P,Bianchi S,Pettersson K,Andersson C

    更新日期:1994-11-01 00:00:00

  • Concentration-dependent effect of bleaching agents on the immunolabelling of interleukin-6, interleukin-17 and CD5-positive cells in the dental pulp.

    abstract:AIM:To evaluate lymphocyte-like cell activation (CD5-positive cells) and the expression of interleukin (IL)-6 and IL-17 in the pulp after tooth bleaching with two concentrations of hydrogen peroxide (H2 O2 ). METHODOLOGY:The right and left maxillary molars from 40 rats were treated randomly with bleaching gel with 20%...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12891

    authors: Benetti F,Gomes-Filho JE,Ferreira LL,Sivieri-Araújo G,Ervolino E,Briso ALF,Cintra LTA

    更新日期:2018-07-01 00:00:00

  • Scanning electron microscopic study of the apical dentine surfaces lased with ND:YAG laser following apicectomy and retrofill.

    abstract::The purpose of this investigation was to study the effects of a Nd:YAG laser on the cut surface of teeth using scanning electron microscopy (SEM). Eighteen single-rooted teeth were cleaned, shaped, and obturated with gutta-percha and root canal sealer. The apical 3 mm of each tooth were resected with a diamond fissure...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1992.tb00758.x

    authors: Stabholz A,Khayat A,Weeks DA,Neev J,Torabinejad M

    更新日期:1992-11-01 00:00:00

  • A comparative analysis of periapical health based on historic and current data.

    abstract:AIM:To compare periapical health using samples from prehistoric and historic periods until the present day, and to emphasize the major risk indicators for apical periodontitis (AP). METHODOLOGY:A comparative survey to assess periapical health was performed on five samples (525 individuals) drawn from different periods...

    journal_title:International endodontic journal

    pub_type: 历史文章,杂志文章

    doi:10.1111/j.1365-2591.2005.00905.x

    authors: Chazel JC,Tramini P,Valcarcel J,Pélissier B

    更新日期:2005-05-01 00:00:00

  • Long-term sealing ability of Resilon apical root-end fillings.

    abstract:AIM:To evaluate ex vivo the long-term sealing ability of the SE Resilon Epiphany system as an apical root-end filling material. METHODOLOGY:A total of 60 standardized horizontal bovine root sections were divided into three groups filled with either gutta-percha with AH 26, tooth-coloured mineral trioxide aggregate (MT...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2009.01583.x

    authors: De Bruyne MA,De Moor RJ

    更新日期:2009-10-01 00:00:00

  • Vertical root fractures in adjacent maxillary premolars: an endodontic-prosthetic perplexity.

    abstract::Vertical root fractures in endodontically treated teeth occur frequently in teeth or roots in which their mesiodistal dimension is narrow, such as the maxillary upper premolars. Two cases of vertical root fractures in two adjacent maxillary reconstructed teeth are presented. As in many cases of vertical root fractures...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1046/j.1365-2591.1998.00129.x

    authors: Tamse A,Zilburg I,Halpern J

    更新日期:1998-03-01 00:00:00

  • Substance P and Calcitonin gene-related peptide expression in human periodontal ligament after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files.

    abstract:AIM:To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. METHODOLOGY:A total of 50 human periodontal ligament samples were obtained from health...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12952

    authors: Caviedes-Bucheli J,Rios-Osorio N,Rey-Rojas M,Laguna-Rivero F,Azuero-Holguin MM,Diaz LE,Curtidor H,Castaneda-Ramirez JJ,Munoz HR

    更新日期:2018-12-01 00:00:00

  • Ex-vivo area-metric analysis of root canal obturation using gutta-percha cones of different taper.

    abstract:AIM:To evaluate the percentage of gutta-percha and sealer-filled canal area when four different types of master gutta-percha cones were used. METHODOLOGY:Sixty mandibular premolars with straight canals were instrumented using System ProTaper rotary instruments with a crown-down technique and assigned to four groups. C...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.2008.01533.x

    authors: Romania C,Beltes P,Boutsioukis C,Dandakis C

    更新日期:2009-06-01 00:00:00

  • Effect of oral premedication on the anaesthetic efficacy of inferior alveolar nerve block in patients with irreversible pulpitis - A systematic review and network meta-analysis of randomized controlled trials.

    abstract::This systematic review (SR; PROSPERO database: CRD42017075160) and network meta-analysis (NMA) identified the most effective oral premedication for anaesthetic success of inferior alveolar nerve blocks (IANB) in cases of irreversible pulpitis. Medline and Ebscohost databases were searched up until 10/2017. Randomized ...

    journal_title:International endodontic journal

    pub_type: 杂志文章,meta分析

    doi:10.1111/iej.12912

    authors: Pulikkotil SJ,Nagendrababu V,Veettil SK,Jinatongthai P,Setzer FC

    更新日期:2018-09-01 00:00:00

  • Cone beam computed tomography in Endodontics - a review of the literature.

    abstract::The use of cone beam computed tomography (CBCT) in the diagnosis and/or management of endodontic problems is increasing and is reflected in the exponential rise in publications on this topic in the last two decades. The aim of this paper is to: (i) Review current literature on the endodontic applications of CBCT; (ii)...

    journal_title:International endodontic journal

    pub_type: 杂志文章,评审

    doi:10.1111/iej.13115

    authors: Patel S,Brown J,Pimentel T,Kelly RD,Abella F,Durack C

    更新日期:2019-08-01 00:00:00

  • XP-endo Finisher R instrument optimizes the removal of root filling remnants in oval-shaped canals.

    abstract:AIM:To evaluate the performance of XP-endo Finisher R instruments when removing root filling remnants from oval-shaped canals using microcomputed tomographic (micro-CT) imaging as the analytical tool. Passive ultrasonic irrigation (PUI) was used as a reference technique for comparison. METHODOLOGY:Twenty mandibular in...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.13077

    authors: De-Deus G,Belladonna FG,Zuolo AS,Cavalcante DM,Carvalhal JCA,Simões-Carvalho M,Souza EM,Lopes RT,Silva EJNL

    更新日期:2019-06-01 00:00:00

  • Comparative evaluation of the shaping ability of ProTaper Next, iRaCe and Hyflex CM rotary NiTi files in severely curved root canals.

    abstract:AIM:To compare the shaping ability of ProTaper Next, iRaCe and Hyflex CM rotary NiTi files during the preparation of severely curved root canals in extracted human molar teeth. METHODOLOGY:Sixty mandibular molars with mesio-buccal canals having angles of curvature ranging from 25° to 35° were divided according to the ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12291

    authors: Saber SE,Nagy MM,Schäfer E

    更新日期:2015-02-01 00:00:00

  • Fracture resistance of root filled premolar teeth restored with direct composite resin with or without cusp coverage.

    abstract:AIM:To evaluate the fracture resistance and fracture patterns of root filled maxillary first premolar teeth (with mesio-occlusal cavity preparation) restored with several composite restoration designs. METHODOLOGY:One hundred extracted sound human maxillary first premolars were randomly divided into five groups. Group...

    journal_title:International endodontic journal

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1365-2591.2011.02005.x

    authors: Xie KX,Wang XY,Gao XJ,Yuan CY,Li JX,Chu CH

    更新日期:2012-06-01 00:00:00

  • Atomic force microscopic evaluation of nanostructure alterations of rotary NiTi instruments after immersion in irrigating solutions.

    abstract:AIM:To compare the effect of 5.25% NaOCl or 17% EDTA solutions on the surface topography and roughness of GTX, TF, RaCe and Hero Shaper instruments using atomic force microscopy (AFM) analysis. METHODOLOGY:Four new files of each brand were subjected to AMF analysis; then each group was subdivided into two subgroups (n...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.12189

    authors: Fayyad DM,Mahran AH

    更新日期:2014-06-01 00:00:00

  • The management of deep carious lesions and the exposed pulp amongst members of two European endodontic societies: a questionnaire-based study.

    abstract:AIMS:To investigate and compare views on management of deep caries and the exposed pulp by Endodontic Society members in Ireland (Irish Endodontic Society [IES]) and Italy (Accademia Italiana di Endodonzia [AIE]). Further aims were to investigate the influence of patient-related factors (age, symptoms) and operator-rel...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/iej.13418

    authors: Careddu R,Plotino G,Cotti E,Duncan HF

    更新日期:2020-09-24 00:00:00

  • Effect of dentine surface treatment on leakage of root fillings with a glass ionomer sealer.

    abstract::The purpose of this study was to observe the quality of seal of the glass ionomer cement, Ketac-Endo, after treatment of the root canal wall. The root canals of 140 extracted human teeth were prepared biomechanically. The root canals were treated with either EDTA or received an intracanal dressing of calcium hydroxide...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1995.tb00298.x

    authors: Holland R,Sakashita MS,Murata SS,Junior ED

    更新日期:1995-07-01 00:00:00

  • The antibacterial properties of four elements released from dental restorative materials.

    abstract::The antibacterial activity of four elements (zinc, copper, fluoride and mercury), previously reported to be released from dental restorative materials, was investigated. This was carried out using two aerobic micro-organisms isolated from dentine beneath cavities in the ferret. These were grown in the presence of the ...

    journal_title:International endodontic journal

    pub_type: 杂志文章

    doi:10.1111/j.1365-2591.1989.tb00499.x

    authors: Nourollahi M,Meryon SD

    更新日期:1989-01-01 00:00:00