Abstract:
AIM:To determine whether nisin, a bacteriocin, would be effective at killing Enterococcus faecalis and Streptococcus gordonii cells in solution and within the root canal system. METHODOLOGY:Bacterial isolates of E. faecalis and S. gordonii were grown from glycerol stocks in closed tubes containing BHY broth at 37 degrees C. The minimum bactericidal concentration (MBC) of nisin for both bacterial species was determined by a microdilution method. Extracted human teeth were decoronated to produce roots of equal length with a single canal and divided into six groups of 10 roots. The canals were prepared to a master apical size 30 file using 0.04 taper Ni-Ti rotary instruments. Bacterial samples of each species were inoculated into three groups of prepared roots and incubated in closed tubes at 37 degrees C for 21 days. The root canals in each group were then medicated with water (control), calcium hydroxide powder mixed with sterile water [Ca(OH)2], or nisin and incubated for a further 7 days. Rotary Ni-Ti files were used to take radicular dentine samples from the walls of each canal which were then incubated in BHY broth for 24 h. Optical density (OD600) readings were taken as a measure of bacterial growth. RESULTS:The MBC of nisin for E. faecalis and S. gordonii was 70 and 20 mg mL(-1) respectively. Calcium hydroxide and nisin medication eradicated infection within the root canal while cells remained viable in the control group. Mean optical density (OD600) readings from canal wall dentine shavings infected with E. faecalis were 1.32 +/- 0.98, 0.73 +/- 0.27 and 0.69 +/- 0.38 for the control, Ca(OH)2 and nisin samples respectively. Corresponding mean readings for S. gordonii were 1.19 +/- 0.18, 0.73 +/- 0.15 and 0.60 +/- 0.29. The Ca(OH)2 and nisin group readings were significantly (P < 0.01) lower than the control for each species as tested by Student's t-test and Mann-Whitney U statistical analysis. Values for Ca(OH)2 and nisin were not significantly (P > 0.01) different. CONCLUSION:Nisin was effective at eradicating E. faecalis and S. gordonii cells in pure culture and was comparable with Ca(OH)2 in the elimination of these species from within the root canal system.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Turner SR,Love RM,Lyons KMdoi
10.1111/j.1365-2591.2004.00846.xkeywords:
subject
Has Abstractpub_date
2004-10-01 00:00:00pages
664-71issue
10eissn
0143-2885issn
1365-2591pii
IEJ846journal_volume
37pub_type
杂志文章abstract:AIM:To present a scientometric analysis of the entire body of scientific publications in the field of vital pulp therapy (VPT) and analyse the research trends and popular topics. METHODOLOGY:A comprehensive literature search was conducted in the Web of Science and Scopus databases on 21 August 2020 to identify all art...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13422
更新日期:2021-02-01 00:00:00
abstract:AIM:To evaluate the torsional properties of pathfinding nickel-titanium (NiTi) rotary instruments manufactured from several NiTi alloys, ProGlider (M-wire), Hyflex GPF (conventional NiTi Wire and controlled memory wire), Logic (conventional NiTi wire and controlled memory wire) and Mtwo (conventional NiTi wire). METHO...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12879
更新日期:2018-06-01 00:00:00
abstract:AIM:To introduce and assess a novel method for measuring washout resistance of cement-based dental materials, including mineral trioxide aggregate (MTA), to qualitatively verify the results with a clinical simulation and to evaluate the washout resistance of a new root-end filling material. METHODOLOGY:A method for as...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02108.x
更新日期:2013-02-01 00:00:00
abstract:AIM:The apical sealing ability of three different endodontic sealers was evaluated in extracted teeth using dye penetration. METHODOLOGY:The root canals of 99 extracted human maxillary central incisors were prepared sequentially 2 mm beyond the apical foramen with a size 55 Nitiflex file. The teeth were divided into t...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2000.00247.x
更新日期:2000-01-01 00:00:00
abstract:AIM:To evaluate the effect of irrigation with 5% sodium hypochlorite (NaOCl) alone and in conjunction with 17% ethylenediaminetetraacetic acid (EDTA) on tooth surface strain (TSS) and to analyse the influence of irrigation time, root morphology and dentine thickness. METHODOLOGY:Thirty-six single-rooted pre-molars wit...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01655.x
更新日期:2010-03-01 00:00:00
abstract:AIM:To evaluate ex vivo the quality of root fillings completed by two thermoplasticized gutta-percha techniques (Thermafil and System B) and a cold gutta-percha technique (single point) by μCT analysis. METHODOLOGY:A total of 30 freshly extracted human single-rooted permanent teeth were selected. Root canals were prep...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01840.x
更新日期:2011-04-01 00:00:00
abstract::The thermal effects of a CO2 laser on the external root surface and inside the root canal were studied in vitro by means of computerized infrared (IR) thermography and a digital thermometer. One-hundred-and-eighty tooth roots with single root canals were irradiated internally and externally with laser power set at 0.5...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00573.x
更新日期:1993-09-01 00:00:00
abstract:AIM:To elucidate the expressions of MMP-8 and MMP-13 in experimentally induced rat periradicular lesions by means of the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining. METHODOLOGY:Thirty rats were used and periradicular lesions in mandibular first molar teeth were establishe...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01880.x
更新日期:2011-08-01 00:00:00
abstract:AIM:To perform multiparametric analysis of the effects of soya milk (SM), whole milk (WM) and Hank's balanced salt solution (HBSS) on the viability of fibroblasts (HGF). The study also aimed to evaluate the influence of these solutions on bovine root dentine according to OH- and PO43- on the surface. METHODOLOGY:The H...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12739
更新日期:2017-12-01 00:00:00
abstract:AIM:The aim of the present study was to identify periapical changes 20-27 years after root-canal treatment. METHODOLOGY:The periapical condition of 265 roots filled by undergraduate students was evaluated in two series of intraoral radiographs taken 10-17 and 20-27 years after treatment. Roots (72) not recorded with a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00568.x
更新日期:2002-09-01 00:00:00
abstract:AIM:To obtain concurrent radicular measurements in the mesiobuccal (MB) and mesiolingual (ML) canals of mandibular first molars using scanned data of micro-computed tomography (μCT) with novel software. METHODOLOGY:The scanned data from 37 mandibular first molar mesial roots were reconstructed and analysed with custom...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12326
更新日期:2015-04-01 00:00:00
abstract::Following extraction of a symptomatic mandibular premolar which had been subjected to two periradicular surgical procedures, significant apical healing was identified histologically. The implications of these findings are discussed in relation to contemporary advocated treatment regimens, case assessment, and interpre...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00280.x
更新日期:1994-11-01 00:00:00
abstract:AIM:The aim of this study is to test the hypothesis that more patients with failed root-canal treatment or other endodontic problems are referred for periradicular surgery rather than nonsurgical re-treatment. METHODOLOGY:Three sets of 100 periapical radiographs representing typical cases referred for surgical treatme...
journal_title:International endodontic journal
pub_type: 杂志文章,多中心研究
doi:10.1046/j.1365-2591.2002.00510.x
更新日期:2002-05-01 00:00:00
abstract:AIM:To determine the influence of filling technique and root canal area on the percentage of gutta-percha (PGP) in laterally compacted root fillings. METHODOLOGY:Sixty extracted canine teeth were accessed and the root canals instrumented to the same size. They were then divided in three groups and filled with laterall...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01575.x
更新日期:2009-08-01 00:00:00
abstract:AIM:To investigate the prevalence of Porphyromonas gingivalis in root canals of infected teeth with periapical abscesses and to investigate the antimicrobial susceptibility of this species to some frequently prescribed antibiotics. METHODOLOGY:Samples were obtained from 70 root canals of abscessed teeth. Microbial sam...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.01053.x
更新日期:2006-01-01 00:00:00
abstract::Ninety-one extracted maxillary premolar teeth were restored with a prefabricated post and amalgam, composite resin or glass-cermet core. Each group was again divided into three groups of 9-13 teeth to be subjected to an increasing load in one of three standardized directions (10, 45 and 90 degrees to the long axis of ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb00774.x
更新日期:1992-05-01 00:00:00
abstract::Models which simulate oral conditions pertaining to endodontic surgical procedures are most suitable if they contain natural teeth and have hard and soft tissues which handle realistically. In addition, the model should possess radiological characteristics similar to bone. A simple custom-built model which fulfils the...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract:AIM:To compare the antibacterial activity of 2-hydroxyisocaproic acid (HICA) with currently used root canal medicaments and to examine their interactions with potential inhibitors in nutrient-deficient and nutrient-rich conditions. METHODOLOGY:First, the antibacterial activity of single concentrations of HICA, calcium...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12465
更新日期:2016-04-01 00:00:00
abstract:AIM:To evaluate the efficacy of a prototype root canal dressing containing surface pre-reacted glass-ionomer (S-PRG) fillers on repairing induced periapical lesions in a rat model. Calcium hydroxide [Ca(OH)2 ] was applied as a comparison in the healing process. METHODOLOGY:The pulp chambers of the maxillary first mola...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13414
更新日期:2021-02-01 00:00:00
abstract::Animal testing is crucial in situations when research on humans is not allowed because of unknown health risks and ethical concerns. The current project aims to develop reporting guidelines exclusively for animal studies in Endodontology, using an established consensus-based methodology. The guidelines have been named...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13477
更新日期:2021-01-15 00:00:00
abstract:AIM:To use micro-computed tomography (μ-CT) to evaluate the amount of calcium hydroxide [Ca(OH)2 ] remaining in the C-shaped root canals of mandibular second molars after attempting to remove it with passive ultrasonic and sonic irrigation. METHODOLOGY:Thirty mandibular second molars, 15 in C1 and 15 in C2 configurati...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12319
更新日期:2015-04-01 00:00:00
abstract:AIM:To assess the free available chlorine concentration (FAC), organic tissue dissolution, and smear layer removal capacity of sodium hypochlorite (NaOCl) alone and when mixtured with etidronate (HEDP) and tetrasodium EDTA (Na4 EDTA), and heated to different temperatures. METHODOLOGY:Mixtures at 1:1 ratio of 5% NaOCl ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13450
更新日期:2020-11-20 00:00:00
abstract:AIM:The aim of this study was to compare in vitro bacterial and dye leakage tests, commonly used to determine the seal of root canal fillings. METHODOLOGY:Ninety-six single-rooted teeth had their crowns removed at the cemento-enamel junction and their roots instrumented to ISO size 60 within 1 mm of the apex. Three gr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00235.x
更新日期:1999-09-01 00:00:00
abstract:AIM:Apatite-forming ability, considered as an index of bioactivity (bond-to-bone ability), was tested on ProRoot MTA cement after immersion in phosphate-containing solution (DPBS). METHODOLOGY:Disk samples were prepared and immersed in DPBS for 10 min, 5 h, 1 and 7 days. The cement surface was studied by attenuated to...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01768.x
更新日期:2010-10-01 00:00:00
abstract:AIM:To compare the efficacy of different solutions (1% sodium hypochlorite, 10% citric acid and 20% EDTA) in the removal of calcium hydroxide from root canals. METHODOLOGY:One hundred single-rooted maxillary incisors were prepared to size 50 and split longitudinally. Two standardized grooves were cut into the apical a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01709.x
更新日期:2010-06-01 00:00:00
abstract:AIM:The aim of this study was to test the null hypothesis that sodium hypochlorite irrigation of root canals does not alter the properties of dentine and contribute to the weakening of root-treated teeth. METHODOLOGY:The effect of two concentrations (0.5%, 5.25%) of sodium hypochlorite (NaOCl) and saline on (i) the el...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2001.00357.x
更新日期:2001-03-01 00:00:00
abstract:AIM:The aim of the present study was to investigate the antimicrobial activity of octenidine on Enterococcus faecalis ATCC 29212 in a dentine block model. METHODOLOGY:Fifty-six root segments of extracted human teeth were infected with E. faecalis for 4 weeks. Octenidine-phenoxyethanol gel (1 : 1) was applied for diffe...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01279.x
更新日期:2007-11-01 00:00:00
abstract::The root canal anatomy of 19 mandibular second molars with C-shaped canals was investigated by rendering the roots transparent and allowing the canal system to be observed by black ink infiltration. The presence of three root canals was most frequent, and lateral canals were found in all roots. Transverse anastomoses ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1990.tb00801.x
更新日期:1990-01-01 00:00:00
abstract::The aim of this study was to assess the shaping ability of the M4 reciprocating handpiece and Safety Hedstrom files in simulated canals. A total of 40 simulated canals of various angles and positions of curvature were prepared with an M4 handpiece using Safety Hedstrom files oriented with the ground, flattened surface...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1997.tb01094.x
更新日期:1997-01-01 00:00:00
abstract:AIM:To investigate the ability of BioRoot RCS, a tricalcium silicate-based root canal sealer and AH Plus to effectively fill the root canals of contralateral teeth using three evaluation methods, and to investigate also the correlation between the methods. METHODOLOGY:The prepared root canals of ten pairs of contralat...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12513
更新日期:2016-08-01 00:00:00