Abstract:
AIM:To elucidate mechanisms by which mineral trioxide aggregate (MTA) suppresses pro-inflammatory cytokine mRNA expression in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. METHODOLOGY:Mineral trioxide aggregate extracts were prepared by immersing set ProRoot MTA in culture medium. RAW264.7 cells were cultured in the presence of LPS and MTA extracts. mRNA expression levels of interleukin (IL)-1α, IL-6, early growth response 2 (Egr2), suppressor of cytokine signalling 3 (Socs3) and IL-10 were quantified with reverse transcription-quantitative polymerase chain reaction. Phosphorylation of nuclear factor-kappa B (NF-κB) p65 in RAW264.7 cells was analysed by Western blotting. Intracellular calcium imaging was performed with Fluo-4 AM. The activity of nuclear factor of activated T cells (NFAT) was determined by luciferase assays. Enforced expression and silencing of Egr2 in RAW264.7 cells were carried out using an expression vector and specific RNAi, respectively. In vivo kinetics of Egr2+ cells in MTA-treated rat molar pulp tissues were examined using immunohistochemistry. Data were analysed by one-way analysis of variance, followed by the Tukey-Kramer test (P < 0.05). RESULTS:Exposure to MTA extracts resulted in reduced mRNA expression levels of IL-1α and IL-6, as well as reduced expression of phosphorylated NF-κB, in LPS-stimulated RAW264.7 cells. Exposure to MTA extracts induced Ca2+ influx, which was blocked by NPS2143, an antagonist of calcium-sensing receptor (CaSR); Ca2+ influx then triggered activation of calcineurin/NFAT signalling and enhanced mRNA expression of Egr2. Enforced expression of Egr2 in RAW264.7 cells promoted the expression of both IL-10 and Socs3. In vivo application of MTA onto rat molar pulp tissue resulted in the appearance of Egr2-expressing cells that coexpressed CD163, a typical M2 macrophage marker. CONCLUSIONS:Mineral trioxide aggregate extracts induced downregulation of IL-1α and IL-6 in LPS-stimulated RAW264.7 cells via CaSR-induced activation of calcineurin/NFAT/Egr2 signalling and subsequent upregulation of IL-10 and Socs3.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Kuramoto M,Kawashima N,Tazawa K,Nara K,Fujii M,Noda S,Hashimoto K,Nozaki K,Okiji Tdoi
10.1111/iej.13386subject
Has Abstractpub_date
2020-12-01 00:00:00pages
1653-1665issue
12eissn
0143-2885issn
1365-2591journal_volume
53pub_type
杂志文章abstract:AIM:To describe the successful non-surgical endodontic management of a permanent maxillary lateral incisor tooth with four root canals. SUMMARY:Aberrant morphology of root canals can occur in any tooth, including maxillary lateral incisors. In the presented case, clinical examination and radiographs clearly depicted t...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01984.x
更新日期:2012-04-01 00:00:00
abstract:AIM:To compare ex vivo the accuracy of two impedance quotient apex locators with different scales and frequencies of the measuring circuit. METHODOLOGY:In each root of 193 extracted human teeth, electronic working length determination (ELD) was carried out with a newly constructed measuring unit. In all cases, ELD was...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.01004.x
更新日期:2005-12-01 00:00:00
abstract:AIM:To compare the penetration resistance (PR) of six thermoplastic gutta-percha (GP) materials against needle penetration at different temperatures. METHODOLOGY:The PR of the following materials based on warm gutta-percha techniques, Beefill GP Cartridge (Beefill), GP Obturator-Hard/Medium/Soft (Dia-H, Dia-M, Dia-S),...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12349
更新日期:2015-06-01 00:00:00
abstract:AIM:This case report demonstrates a positive outcome of the adjuvant use of fragile fracture (FF), which is a technique used to harvest dental pulp stem cells (DPSCs), and platelet-rich plasma (PRP) in a mandibular premolar (tooth 44) with a completely formed root that was transplanted into a surgically created socket ...
journal_title:International endodontic journal
pub_type:
doi:10.1111/iej.13230
更新日期:2020-03-01 00:00:00
abstract:AIM:To assay the toxicity of the single-methacrylate-based sealer urethane dimethacrylate (UDMA) (EndoRez) in terms of cell growth and pro-inflammatory cytokines release, in expanded ex vivo human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPDLSCs), human gingival fibroblasts (hGFs) and hum...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12230
更新日期:2014-09-01 00:00:00
abstract:AIM:To present examples of radiographic image reconstructions in endodontic treatment, to discuss the potential problems arising from the use of image processing software and to mention some current methods to prevent such counterfeit utilization of digital endodontic radiographs. SUMMARY:Conventional Ultra-Speed peri...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.0143-2885.2004.00780.x
更新日期:2004-03-01 00:00:00
abstract:AIM:To compare the antibacterial activity of 2-hydroxyisocaproic acid (HICA) with currently used root canal medicaments and to examine their interactions with potential inhibitors in nutrient-deficient and nutrient-rich conditions. METHODOLOGY:First, the antibacterial activity of single concentrations of HICA, calcium...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12465
更新日期:2016-04-01 00:00:00
abstract:AIM:To evaluate the neurotoxic effects of two endodontic sealers, AH-26 and Roth 801, on firing excitability and action potential configuration of F1 neural cells in the suboesophageal ganglia of Helix aspersa. METHODOLOGY:A conventional intracellular current clamp technique was used to study the blocking effects of A...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2003.00751.x
更新日期:2003-12-01 00:00:00
abstract::Teflon tubes containing freshly mixed, polymerized Hydron root canal filling material, fully set AH26 or Teflon were implanted into the mandible of guinea pigs and assessed histologically at 2 days, 1, 2, 4, 12 and 26 weeks. None of the materials tested elicited signs of overt or significant tissue damage, and polymer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb00749.x
更新日期:1992-07-01 00:00:00
abstract:AIM:To investigate the composition of materials and leachate of a hydrated prototype cement composed of tricalcium silicate and radiopacifier and compare this to other tricalcium silicate-based cements (Biodentine and Bioaggregate) to assess whether the additives in the proprietary brand cements affect the hydration of...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12039
更新日期:2013-07-01 00:00:00
abstract::The objective of this study was to compare the adaptation of mechanically softened gutta-percha to the root canal wall in the presence and absence of smear layer. The root canals of 20 freshly extracted human maxillary incisors were cleaned and shaped. Prior to obturation, 10 root canals were irrigated with 20 ml of 5...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1995.tb00312.x
更新日期:1995-09-01 00:00:00
abstract:AIM:To evaluate the effect of a glide path on the amount of apically extruded debris during canal preparation using single-file systems in curved canals. METHODOLOGY:Ninety extracted mandibular molar teeth were randomly assigned to six groups (n = 15 for each group) for canal instrumentation. Endodontic access cavitie...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12484
更新日期:2016-06-01 00:00:00
abstract:AIM:To determine the influence of filling technique and root canal area on the percentage of gutta-percha (PGP) in laterally compacted root fillings. METHODOLOGY:Sixty extracted canine teeth were accessed and the root canals instrumented to the same size. They were then divided in three groups and filled with laterall...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01575.x
更新日期:2009-08-01 00:00:00
abstract::In an effort to simulate the infected dental root canal, bacteria were enclosed in polyethylene tubes that were subcutaneously implanted into rats. Four different bacterial combinations, selected from nine oral species, were tested. The contents of 144 inoculated and implanted 'root canals' were analysed after 2 and 4...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1989.tb00931.x
更新日期:1989-11-01 00:00:00
abstract:AIM:To evaluate the accuracy of the Root ZX and Elements-Diagnostic electronic apex locators when compared with radiographs for locating the canal terminus or minor foramen. METHODOLOGY:The canal terminus of 482 canals in 160 maxillary and mandibular teeth was located in vivo with both locators and radiographically. A...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01620.x
更新日期:2010-01-01 00:00:00
abstract:AIM:To compare the shaping ability of ProTaper Next, iRaCe and Hyflex CM rotary NiTi files during the preparation of severely curved root canals in extracted human molar teeth. METHODOLOGY:Sixty mandibular molars with mesio-buccal canals having angles of curvature ranging from 25° to 35° were divided according to the ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12291
更新日期:2015-02-01 00:00:00
abstract:AIM:To compare the fracture resistance of thin-walled roots after restoration with different types of post systems. METHODOLOGY:One hundred and sixty-five decoronated maxillary central incisors were root filled and randomly assigned to three groups with respect to the remaining dentine thickness of root (1.0 mm, 1.5 m...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01609.x
更新日期:2009-11-01 00:00:00
abstract::Animal testing is crucial in situations when research on humans is not allowed because of unknown health risks and ethical concerns. The current project aims to develop reporting guidelines exclusively for animal studies in Endodontology, using an established consensus-based methodology. The guidelines have been named...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13477
更新日期:2021-01-15 00:00:00
abstract:AIM:To develop a precise and simple mounting model (MM) for evaluating apex locators and to compare the repeatability of the MM with a conventional visual method (VM). METHODOLOGY:Electronic working length determination was performed in 32 maxillary central incisors using two methods: (i) the MM method and (ii) a conv...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01052.x
更新日期:2006-02-01 00:00:00
abstract:AIM:The aim of this study is to test the hypothesis that more patients with failed root-canal treatment or other endodontic problems are referred for periradicular surgery rather than nonsurgical re-treatment. METHODOLOGY:Three sets of 100 periapical radiographs representing typical cases referred for surgical treatme...
journal_title:International endodontic journal
pub_type: 杂志文章,多中心研究
doi:10.1046/j.1365-2591.2002.00510.x
更新日期:2002-05-01 00:00:00
abstract::Irrigation solutions and intracanal medicaments are used within the root canal to clean and aid in disinfecting the dentinal walls. Although these materials are intended to be contained within the root canal, they invariably contact the periapical tissues, either through inadvertent extrusion through the apex or leach...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00631.x
更新日期:2003-02-01 00:00:00
abstract:AIM:To determine whether nisin, a bacteriocin, would be effective at killing Enterococcus faecalis and Streptococcus gordonii cells in solution and within the root canal system. METHODOLOGY:Bacterial isolates of E. faecalis and S. gordonii were grown from glycerol stocks in closed tubes containing BHY broth at 37 degr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00846.x
更新日期:2004-10-01 00:00:00
abstract:AIM:To investigate the prevalence of apical periodontitis and the quality of root canal treatment in an adult Turkish population who visited a university dental clinic. SUMMARY:The periapical status of 8863 teeth belonging to 375 patients was evaluated by two pre-calibrated observers from panoramic radiographs. The pr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01217.x
更新日期:2007-02-01 00:00:00
abstract:AIM:To evaluate ex vivo the accuracy of two electronic apex locators during root canal length determination in primary incisor and molar teeth with different stages of physiological root resorption. METHODOLOGY:One calibrated examiner determined the root canal length in 17 primary incisors and 16 primary molars (total...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01366.x
更新日期:2008-04-01 00:00:00
abstract:AIM:To determine retrospectively the long-term radiographic outcome of root canal treatments and root canal retreatments with unintentional root canal overfilling. METHODOLOGY:A total of 220 root canal treatments (143 primary/77 retreatments) with postoperative unintentional canal overfilling and performed by two endo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13209
更新日期:2020-01-01 00:00:00
abstract::Root-canal-filling materials are either placed directly onto vital periapical tissues or may leach through dentine. The tissue response to these materials therefore becomes important and may influence the outcome of endodontic treatment. This paper is a review of the biocompatibility of contemporary orthograde and ret...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00637.x
更新日期:2003-03-01 00:00:00
abstract:AIM:To determine the cleaning effectiveness and shaping ability of ProTaper and RaCe nickel-titanium rotary instruments during the preparation of curved root canals in extracted human teeth. METHODOLOGY:A total of 48 root canals of mandibular and maxillary molars with curvatures ranging between 25 degrees and 35 degre...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.0143-2885.2004.00783.x
更新日期:2004-04-01 00:00:00
abstract::The aim of designing and fabricating the surgical templates was to assist the surgical component of premolar transplantation, by establishing and replicating the root dimensions of the donor premolar tooth. The correct template could be used to assess the socket preparation (width and depth) prior to placement of the ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02077.x
更新日期:2012-11-01 00:00:00
abstract:AIM:To present the successful endodontic and periodontal management of a two rooted maxillary lateral incisor tooth with a complex radicular lingual groove and severe periodontal destruction using spiral computed tomography as a diagnostic aid. SUMMARY:A 30-year-old male patient presented with a chief complaint of mob...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01868.x
更新日期:2011-06-01 00:00:00
abstract:AIM:To evaluate ex vivo the incidence of defects in root dentine before and after root canal preparation and filling. METHODOLOGY:Eighty extracted mandibular premolars were divided equally in four groups. Group 1 was left unprepared. All other root canals were prepared using Gates Glidden drills and System GT files up...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01502.x
更新日期:2009-03-01 00:00:00