Abstract:
AIM:To investigate the role of the Ca(2+) -Mg(2+) ion channel TRPM7 in the proliferation, migration and osteogenic differentiation of human dental pulp stem cells (hDPSCs). METHODOLOGY:Immunohistochemistry was used to localize expression of TRPM7 in human dental pulp tissues and in cultured hDPSCs. Isolated hDPSCs were infected with recombinant lentiviruses expressing short hairpin RNA (shRNA) specific for TRPM7, or control shRNA, in order to suppress TRPM7 mRNA expression and investigate its functional role. The proliferation of the shRNA-infected hDPSCs was evaluated using both an MTT assay to measure viable cell numbers and cell cycle analysis. Cell migration was assessed using a transwell assay. The dynamic mRNA expression of TRPM7 during osteogenic differentiation of hDPSCs and the effect of shRNA specific for TRPM7 on hDPSC osteogenic differentiation were evaluated by real-time PCR. RESULTS:TRPM7 expression was widespread in human dental pulp tissue and was detected mainly in the cytomembrane and cytoplasm of hDPSCs. Suppression of TRPM7 inhibited both the proliferation and the migratory capacity of hDPSCs. TRPM7 mRNA expression was elevated during osteogenic differentiation of hDPSCs. TRPM7-specific shRNA inhibited osteogenic differentiation of hDPSCs, with downregulated mRNA expression of the osteogenic markers alkaline phosphatase (ALP), dentine sialophosphoprotein (DSPP), bone sialoprotein (BSP), runt-related transcription factor (RUNX2) and osterix (OSX). CONCLUSIONS:TRPM7 was involved in the regulation of hDPSC proliferation, migration and osteogenic differentiation and may play a role in the dental pulp repair process.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Cui L,Xu SM,Ma DD,Wu BLdoi
10.1111/iej.12193subject
Has Abstractpub_date
2014-06-01 00:00:00pages
583-93issue
6eissn
0143-2885issn
1365-2591journal_volume
47pub_type
杂志文章abstract::A case is described in which root canal treatment with calcium hydroxide was used successfully to repair a fracture site with internal resorption of the tooth. ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1996.tb01404.x
更新日期:1996-11-01 00:00:00
abstract:AIM:To evaluate the adhesive strength of Resilon to Next root canal sealant (Heraeus-Kulzer), a methacrylate-based root canal sealer, using a modified microshear bond testing design. METHODOLOGY:Flat Resilon surfaces of different roughnesses (smooth surface and surface roughness equivalent to 320-grit and 180-grit) we...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.01012.x
更新日期:2005-10-01 00:00:00
abstract:AIM:To compare torque and angle of rotation at fracture of new and used K3 rotary instruments. The relation between size of instrument and torque at fracture was also investigated. METHODOLOGY:Nickel-titanium (Ni-Ti).06 K3 rotary instruments were used in a crown-down manner at 300 r.p.m. to prepare canals in resin blo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00732.x
更新日期:2003-11-01 00:00:00
abstract::The aim of this study was to assess the shaping ability of the M4 reciprocating handpiece and Safety Hedstrom files in simulated canals. A total of 40 simulated canals of various angles and positions of curvature were prepared with an M4 handpiece using Safety Hedstrom files oriented with the ground, flattened surface...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1997.tb01094.x
更新日期:1997-01-01 00:00:00
abstract:AIM:To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. METHODOLOGY:A total of 50 human periodontal ligament samples were obtained from health...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12952
更新日期:2018-12-01 00:00:00
abstract::The use of a resin-based glass ionomer as an endodontic sealer was studied in vitro. The root canals of extracted single-rooted human teeth with mature apices were prepared using a stepback technique. Subsequently, the smear layer was removed with 40% citric acid and the canals obturated using lateral condensation of ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb01156.x
更新日期:1992-09-01 00:00:00
abstract:AIM:To investigate interfacial stress distribution in restored root filled teeth with various post lengths and crown heights. METHODOLOGY:Three-dimensional mathematical models of a root filled mandibular premolar tooth were constructed. Parts of the tooth structures were replaced with ceramic crowns having three crown...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12397
更新日期:2015-11-01 00:00:00
abstract:AIM:To investigate the impact of three irrigation protocols, activated by three different methods, on mature biofilms of Enterococcus faecalis in vitro. METHODOLOGY:Root canals in 280 single-rooted teeth were instrumented using a rotary Ni-Ti system. Biofilms of E. faecalis were generated based on a previously establi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12354
更新日期:2015-06-01 00:00:00
abstract:AIM:To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infections and apical periodontitis, and to assess the influence of calcium hydroxide medication [Ca(OH)2 ], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and R...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13403
更新日期:2021-01-01 00:00:00
abstract:AIM:To evaluate connective tissue reactions to iRoot SP (Innovative Bioceramics, Vancouver, BC, Canada), mineral trioxide aggregate (MTA) Fillapex (FLPX) (Angelus Soluções Odontológicas, Londrina, Brazil), DiaRoot Bioaggregate (DiaDent Group International, Burnaby, BC, Canada) and white MTA (Angelus, Londrina, Brazil) ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12203
更新日期:2014-07-01 00:00:00
abstract:AIM:To compare the toxicity of methacrylate resin-based root canal sealers with sealers based on epoxy resin and silicone by two-well established cell culture methods. METHODOLOGY:Specimens of AH Plus, EndoREZ, RoekoSeal and Epiphany were prepared for direct contact in the Millipore filter diffusion test and as extrac...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01321.x
更新日期:2008-01-01 00:00:00
abstract:AIM:To evaluate the influence of delayed scanning on images obtained with two PSPs digital systems and on the diagnostic accuracy of vertical root fracture (VRF) by means of objective and subjective analyses. METHODOLOGY:Forty single-rooted human teeth were divided into two groups, one without VRFs and another with VR...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12547
更新日期:2016-10-01 00:00:00
abstract:AIM:To evaluate the effect of citric acid, ethylenediaminetetraacetic acid (EDTA) and ethylenediaminetetraacetic acid plus Cetavlon (EDTAC) solutions on the microhardness of human root canal dentine. METHODOLOGY:Sixteen maxillary human canines were sectioned transversely at the cemento-enamel junction and the crowns w...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2006.01094.x
更新日期:2006-05-01 00:00:00
abstract:AIM:To evaluate clinically the antibacterial effects of root canal treatment procedures using molecular microbiology analyses. METHODOLOGY:Samples were taken from 14 necrotic root canals of teeth with apical periodontitis before (S1) and after instrumentation with NaOCl irrigation (S2), a final rinse with chlorhexidin...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02111.x
更新日期:2013-03-01 00:00:00
abstract:AIM:To report a case of reparative bone-like tissue formation in the tooth of a patient with systemic sclerosis. SUMMARY:A 58-year-old Japanese female patient with systemic sclerosis was referred because of tooth fracture. Cone beam computerized tomography (CBCT) revealed multiple root resorption and the unclear trans...
journal_title:International endodontic journal
pub_type:
doi:10.1111/iej.12914
更新日期:2018-09-01 00:00:00
abstract:AIM:To evaluate the biocompatibility of two pastes designed to fill the root canals of primary teeth. METHODOLOGY:A study group of 54 mice received subcutaneous tissue implants of polyethylene tubes containing CTZ or calcium hydroxide paste or, as a negative control, empty tubes. Biocompatibility was evaluated on days...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12328
更新日期:2015-05-01 00:00:00
abstract:AIM:To evaluate the influence of root canal form on the sealing ability of two root canal sealers. METHODOLOGY:Twenty radiographically confirmed straight and 20 curved root canals were prepared with a stepback hand filing technique. Root canal aberrations created during preparation were determined by the use of double...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01079.x
更新日期:2006-04-01 00:00:00
abstract:AIM:The purpose of this study was to evaluate the influence of rotational speed, torque, and operator experience with a specific Ni-Ti rotary instrumentation technique on the incidence of locking, deformation and separation of instruments. METHODOLOGY:ProFile Ni-Ti rotary instruments (PRI) sizes 40-15 with a 6% taper ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2001.00352.x
更新日期:2001-01-01 00:00:00
abstract:AIM:To apply a standardized protocol for the orofacial evaluation of two adult siblings (one male and one female) with Hereditary Sensory Radicular Neuropathy (HSRN) that presented with dental problems. SUMMARY:The systematic evaluation consisted of (a) clinical questionnaire; (b) radiographs [orthopantomography and c...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01160.x
更新日期:2006-11-01 00:00:00
abstract:AIM:To investigate the effects of vascular endothelial growth factor A (VEGFA) and the underlying molecular mechanisms on the migration of human dental pulp stem cells (hDPSCs). METHODOLOGY:The expression of VEGFA in inflammatory pulp tissue and lipopolysaccharide (LPS)-stimulated dental pulp cells was examined by imm...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13179
更新日期:2019-12-01 00:00:00
abstract:AIM:To develop a convenient method for the localization and quantification of live and dead bacteria in human ex vivo mineralized dentinal tubules. METHODOLOGY:The roots from human single-rooted teeth (n = 12) were infected with Enterococcus faecalis V583 and either treated with calcium hydroxide paste or left untreat...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01867.x
更新日期:2011-07-01 00:00:00
abstract:CASE REPORTS:The problem of thermal sensitivity following non-surgical root-canal treatment is explored and case reports are presented. Possible causes for post-treatment discomfort from endodontic and restorative aetiologies are discussed, as are the mechanisms to explain the patients' painful experiences. Treatment o...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00197.x
更新日期:1999-03-01 00:00:00
abstract:AIM:To present a scientometric analysis of the entire body of scientific publications in the field of vital pulp therapy (VPT) and analyse the research trends and popular topics. METHODOLOGY:A comprehensive literature search was conducted in the Web of Science and Scopus databases on 21 August 2020 to identify all art...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13422
更新日期:2021-02-01 00:00:00
abstract:AIM:To compare the penetration resistance (PR) of six thermoplastic gutta-percha (GP) materials against needle penetration at different temperatures. METHODOLOGY:The PR of the following materials based on warm gutta-percha techniques, Beefill GP Cartridge (Beefill), GP Obturator-Hard/Medium/Soft (Dia-H, Dia-M, Dia-S),...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12349
更新日期:2015-06-01 00:00:00
abstract::The use of cone beam computed tomography (CBCT) in the diagnosis and/or management of endodontic problems is increasing and is reflected in the exponential rise in publications on this topic in the last two decades. The aim of this paper is to: (i) Review current literature on the endodontic applications of CBCT; (ii)...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13115
更新日期:2019-08-01 00:00:00
abstract:AIM:To clarify the mechanisms of inflammatory cell migration in human periapical granulomas by examining vascular endothelial (VE) cadherin and inducible nitric oxide synthase (iNOS)-producing cells. METHODOLOGY:Periapical tissues were obtained from patients during endodontic surgery and were divided into two portions...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01068.x
更新日期:2006-03-01 00:00:00
abstract::The effect on coronal leakage of the use of either a single gutta-percha cone or cold lateral condensation of gutta-percha, with a glass ionomer sealer cement was studied. In addition, the effect of the removal of the smear layer was also determined. Eighty-eight single-rooted teeth were prepared chemomechanically usi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00255.x
更新日期:1994-07-01 00:00:00
abstract:AIM:To investigate an immortalized murine odontoblast cell line as a potential alternative for experimental studies on dentinogenesis. METHODOLOGY:The MO6-G3 cell line was investigated morphologically over 3, 7, 11 and 42 days of culture, using histochemical localization of dentine sialoprotein (DSP), alkaline phospha...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01089.x
更新日期:2006-06-01 00:00:00
abstract:AIM:To evaluate the effect of three nickel-titanium (Ni-Ti) rotary systems with varying tapers on stress distribution and to analyse potential fracture patterns as well as the volume of fracture-susceptible regions in two-rooted maxillary premolars. METHODOLOGY:The root canals of three single-rooted premolars were pre...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12770
更新日期:2018-05-01 00:00:00
abstract:AIM:To evaluate the response of the apical and periapical tissues of dog teeth with apical periodontitis after one-session root canal treatment with and without antimicrobial photodynamic therapy (aPDT) compared with the use of an intracanal dressing. METHODOLOGY:Sixty root canals with an induced periapical lesion wer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12486
更新日期:2016-06-01 00:00:00