Abstract:
AIM:To assay the toxicity of the single-methacrylate-based sealer urethane dimethacrylate (UDMA) (EndoRez) in terms of cell growth and pro-inflammatory cytokines release, in expanded ex vivo human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPDLSCs), human gingival fibroblasts (hGFs) and human osteoblasts (hOSTs). METHODOLOGY:Dental pulp and periodontal ligament stem cells, osteoblasts and fibroblasts were derived from five young donors. After in vitro isolation, hDPSCs, hPDLSCs, hGFs and hOSTs were seeded to resin-based sealers for 24, 48, 72 h up to 1 week. The morphological features and the cell growth and the release of pro-inflammatory interleukin (IL)6, IL8, IL12 and tumour necrosis factor (TNF) α were analysed. Differences in cell growth and in interleukin secretion were analysed for statistical significance with two-way anova tests for multiple comparisons. RESULTS:Exposure to endodontic sealer based on UDMA resulted in a 50% decrease in survival oral cells at 24 h of incubation. No evident morphological changes were present in cell cultures examined. After 48 h, 72 h and 1-week culture time, a progressive cell growth was evident. A significant up-regulation of IL6, IL8, IL12 and TNFα cytokines in cells in contact with the dental sealer compared to the control was observed. CONCLUSION:In vitro, EndoRez interacted with primary human hDPSCs, hPDLSCs, hGFs and hOSTs causing damage to biological system evidenced through cell growth inhibition and up-regulation of IL6, IL8, IL12 and TNFα proinflammatory mediators.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Diomede F,Caputi S,Merciaro I,Frisone S,D'Arcangelo C,Piattelli A,Trubiani Odoi
10.1111/iej.12230subject
Has Abstractpub_date
2014-09-01 00:00:00pages
864-72issue
9eissn
0143-2885issn
1365-2591journal_volume
47pub_type
杂志文章abstract:AIM:To evaluate the resistance to ex vivo bacterial leakage over a 40-day period of root canal fillings with five new root canal sealers: RC Sealer, Epiphany, EndoREZ, GuttaFlow and Acroseal, compared with Apexit, AH Plus and RoekoSeal. METHODOLOGY:One hundred and forty-four single rooted human teeth were divided rand...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01509.x
更新日期:2009-04-01 00:00:00
abstract:AIM:To report clinical complications (pain, necrotic gingival tissue and bone sequestration) resulting from accidental injection of sodium hypochlorite. SUMMARY:Root canal treatment is a routine clinical procedure with few reported complications. Sodium hypochlorite (NaOCl) is commonly used as an irrigant during the p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01340.x
更新日期:2008-03-01 00:00:00
abstract:AIM:To evaluate the influence of root canal form on the sealing ability of two root canal sealers. METHODOLOGY:Twenty radiographically confirmed straight and 20 curved root canals were prepared with a stepback hand filing technique. Root canal aberrations created during preparation were determined by the use of double...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01079.x
更新日期:2006-04-01 00:00:00
abstract:AIM:To characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. METHODOLOGY:Apical lesion samples were collected from 12 patients (23-80 years old) who visited the Kyushu University Hospital for apicectomy with persistent periapical lesions assoc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12361
更新日期:2015-08-01 00:00:00
abstract:AIM:To evaluate the effect of multiple refreshment/activation cycles and temperature on the reaction rate of sodium hypochlorite (NaOCl) with bovine dentine during ultrasonic activated irrigation (UAI) under laboratory conditions. METHODOLOGY:The root canal walls of 24 standardized root canals in bovine incisors were ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12125
更新日期:2014-02-01 00:00:00
abstract:AIM:The aim of this study was to determine the influence of the width of apical root canals and the depth of heat application during warm vertical compaction on the percentage of gutta-percha-filled area (PGFA) in the apical root canal. METHODOLOGY:Two groups of extracted human canines (total 60) were instrumented and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00522.x
更新日期:2002-06-01 00:00:00
abstract:AIM:To compare the effect of 5.25% NaOCl or 17% EDTA solutions on the surface topography and roughness of GTX, TF, RaCe and Hero Shaper instruments using atomic force microscopy (AFM) analysis. METHODOLOGY:Four new files of each brand were subjected to AMF analysis; then each group was subdivided into two subgroups (n...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12189
更新日期:2014-06-01 00:00:00
abstract:AIM:To develop and preliminarily evaluate a new screening instrument for atypical odontalgia (AO) or persistent dentoalveolar pain disorder (PDAP). To evaluate the instrument's performance in detecting AO/PDAP amongst a heterogeneous group of orofacial pain conditions and pain-free controls and empirically compare its ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13017
更新日期:2019-03-01 00:00:00
abstract:AIM:To assess the long-term outcome of root filled teeth in a general adult population examined in 1997, 2003 and 2008. METHODOLOGY:The study provides longitudinal, observational information on endodontic status for 327 randomly selected individuals from a general Danish population receiving three consecutive full-mou...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12245
更新日期:2014-10-01 00:00:00
abstract:AIM:To investigate the cytotoxicity of a modified salt solution (MSS) and evaluate the antimicrobial properties of MSS on in vitro biofilm models. METHODOLOGY:In a metabolic assay, fibroblasts derived from periodontal ligaments (PDL) of human extracted teeth were cultured and challenged with MSS or controls. Then, in ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12294
更新日期:2015-02-01 00:00:00
abstract:AIM:The apical sealing ability of three different endodontic sealers was evaluated in extracted teeth using dye penetration. METHODOLOGY:The root canals of 99 extracted human maxillary central incisors were prepared sequentially 2 mm beyond the apical foramen with a size 55 Nitiflex file. The teeth were divided into t...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2000.00247.x
更新日期:2000-01-01 00:00:00
abstract:AIM:The aim of this study was to compare two protocols to examine bacterial colonization in teeth associated with chronic apical periodontitis with acute episodes (ap), using light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). METHODOLOGY:Nine root samples (seven teeth...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2009.01594.x
更新日期:2009-10-01 00:00:00
abstract:AIM:The present randomized, controlled prospective study evaluated the histomorphological response of human dental pulps capped with two grey mineral trioxide aggregate (MTA) compounds. METHODOLOGY:Pulp exposures were performed on the occlusal floor of 40 human permanent pre-molars. The pulp was capped either with Pro...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2008.01485.x
更新日期:2009-02-01 00:00:00
abstract:AIM:To identify the suitable ratio of alternative radiopacifiers to impart the necessary radiopacity to calcium silicate cements (CSC) and assess the purity of the radiopacifying agents. METHODOLOGY:Alternative radiopacifying materials for incorporation into CSC included barium sulphate, titanium oxide, zinc oxide, go...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01621.x
更新日期:2010-01-01 00:00:00
abstract:AIM:To determine the pain experienced by patients during root canal treatment and to correlate with age and gender, pulpal diagnosis, previous periapical status, dental characteristics and length of treatment. METHODOLOGY:One hundred and seventy-six patients (68 men and 108 women), with ages ranged from 6 to 83 years,...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01562.x
更新日期:2009-07-01 00:00:00
abstract:AIM:To evaluate the physical and mechanical properties of two cements with a similar chemical composition to mineral trioxide aggregate and to attempt to improve their properties by producing a composite material. METHODOLOGY:Two cement types were used: a mixture of calcium sulpho-aluminate cement and Portland cement ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01435.x
更新日期:2008-10-01 00:00:00
abstract:AIM:This randomized, double-blinded, clinical trial evaluated the effect of oral premedication of piroxicam, prednisolone, dexamethasone or placebo on postoperative pain after single-visit root canal treatment in teeth with symptomatic irreversible pulpitis and symptomatic apical periodontitis. METHODOLOGY:The trial i...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/iej.13416
更新日期:2021-02-01 00:00:00
abstract:AIM:To assess and correlate three distinct states of severely painful endodontically derived inflammation with their depiction on periapical radiographs using periapical index (PAI) scores. METHODOLOGY:During a period of 15 months, 368 consecutively enrolled patients with suspected endodontic emergency conditions were...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13407
更新日期:2021-02-01 00:00:00
abstract:AIM:To compare the shaping ability of ProTaper Next, iRaCe and Hyflex CM rotary NiTi files during the preparation of severely curved root canals in extracted human molar teeth. METHODOLOGY:Sixty mandibular molars with mesio-buccal canals having angles of curvature ranging from 25° to 35° were divided according to the ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12291
更新日期:2015-02-01 00:00:00
abstract:AIM:To compare in vitro the cleanliness of root canal walls in oval-shaped root canals following automated or manual instrumentation. METHODOLOGY:Forty-five oval-shaped single-rooted maxillary and mandibular premolars with straight canals were divided into three groups of 15. Automated canal preparation was performed ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.00938.x
更新日期:2005-06-01 00:00:00
abstract:AIM:To provide insight into the principles of operation of electronic apex locators, determine optimal measuring parameters of the impedance ratio method, and to evaluate its accuracy. METHODOLOGY:Electrical impedance was measured ex vivo on 14 extracted human teeth using a QuadTech 1920 precision impedance analyzer. ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01589.x
更新日期:2009-09-01 00:00:00
abstract:AIMS:To (i) determine the prevalence of persistent dento-alveolar pain following nonsurgical and/or surgical endodontic treatment conducted in a teaching dental hospital and (ii) identify the risk factors associated with persistent pain after apparently successful root canal treatment. STUDY DESIGN:A total of 175 pati...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00923.x
更新日期:2005-03-01 00:00:00
abstract:AIM:To compare the push-out bond strength exhibited by root fillings performed with either C-Point and Endosequence® BC sealer™ (BC Sealer) or gutta-percha and AH Plus® after the instrumentation of oval canals with either the Self-Adjusting File (SAF) System or WaveOne (WO) reciprocating file. METHODOLOGY:Eighty extra...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12455
更新日期:2016-04-01 00:00:00
abstract::The assurance of the quality of a service rendered by a member of the dental profession is an essential feature of any system of peer review in dentistry. This document addresses two essential elements: (i) appropriateness of treatment modality and (ii) quality or level of treatment rendered. In revising these guideli...
journal_title:International endodontic journal
pub_type: 共识发展会议,杂志文章,实务指引
doi:10.1111/j.1365-2591.2006.01180.x
更新日期:2006-12-01 00:00:00
abstract:AIM:To investigate the use of a zinc oxide/zinc sulphate-based cement (Coltosol(®) F, Coltène Whaledent, Cuyahoga Falls, OH, USA) as a temporary filling material during multiple-visit root canal treatments and the occurrence of cracks within the filling material or the tooth. METHODOLOGY:Root canals of one hundred and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12280
更新日期:2015-01-01 00:00:00
abstract::In an effort to simulate the infected dental root canal, bacteria were enclosed in polyethylene tubes that were subcutaneously implanted into rats. Four different bacterial combinations, selected from nine oral species, were tested. The contents of 144 inoculated and implanted 'root canals' were analysed after 2 and 4...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1989.tb00931.x
更新日期:1989-11-01 00:00:00
abstract::Chelating agents were introduced into endodontics as an aid for the preparation of narrow and calcified root canals in 1957 by Nygaard-Østby. A liquid solution of ethylenediaminetetraacetic acid (EDTA) was thought to chemically soften the root canal dentine and dissolve the smear layer, as well as to increase dentine ...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2591.2003.00754.x
更新日期:2003-12-01 00:00:00
abstract:LITERATURE REVIEW:Inter-appointment flare-up is characterized by the development of pain, swelling or both, following endodontic intervention. The causative factors of flare-ups encompass mechanical, chemical and/or microbial injury to the pulp or periradicular tissues. Of these factors, microorganisms are arguably the...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00671.x
更新日期:2003-07-01 00:00:00
abstract::A light-cured ionomer cement was investigated as a retrograde root seal, without a retrograde cavity. This was compared with the material used in a retrograde cavity, and with a conventional glass ionomer cement, as a seal. The adaptation and sealing ability of the test materials were assessed using a confocal optical...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00562.x
更新日期:1993-07-01 00:00:00
abstract:AIM:To describe unusual variations in the root morphology and root canal systems of mandibular first and second premolar teeth extracted for orthodontic reasons. SUMMARY:Normally mandibular first and second premolar teeth have single roots with single canals. A 15-year-old patient presented for orthodontic treatment a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01451.x
更新日期:2008-11-01 00:00:00