Abstract:
AIM:To examine the role of stromal cell-derived factor 1 (SDF-1) signalling during odontogenic differentiation in human dental pulp cells (HDPCs). METHODOLOGY:Human dental pulp cells were treated with differentiation medium, recombinant human SDF-1, neutralizing antibody for SDF-1 or CXCR4, pertussis toxin (PTX) and AMD3100. The expression of SDF-1 and its receptor chemokine receptor type 4 (CXCR4) was measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Odontoblastic differentiation was determined using alkaline phosphatase (ALP) activity assay, mineralized nodule formation and marker mRNAs by RT-PCR. RESULTS:Marked upregulation of SDF-1 and CXCR4 mRNA and protein was observed in cells grown 7 days in osteogenic induction medium. The addition of recombinant human SDF-1 to HDPCs significantly (P < 0.05) increased ALP activity, mineralized nodule formation and odontoblast marker mRNAs in a dose-dependent manner. Blocking SDF-1 signalling using antibodies against SDF-1 or CXCR4, or the G-protein-coupled receptor inhibitor PTX, and CXCR4 inhibitor AMD3100, strongly suppressed induction of odontogenic differentiation in HDPCs. CONCLUSIONS:Odontoblastic differentiation was stimulated by SDF-1 activation and repressed by SDF-1/CXCR4 inhibition. Thus, SDF-1/CXCR4 signalling may be a new therapeutic target and strategy to promote repair and regeneration in endodontics.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Kim DS,Kim YS,Bae WJ,Lee HJ,Chang SW,Kim WS,Kim ECdoi
10.1111/iej.12182subject
Has Abstractpub_date
2014-06-01 00:00:00pages
534-41issue
6eissn
0143-2885issn
1365-2591journal_volume
47pub_type
杂志文章abstract:AIM:To develop a convenient method for the localization and quantification of live and dead bacteria in human ex vivo mineralized dentinal tubules. METHODOLOGY:The roots from human single-rooted teeth (n = 12) were infected with Enterococcus faecalis V583 and either treated with calcium hydroxide paste or left untreat...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01867.x
更新日期:2011-07-01 00:00:00
abstract:AIM:To investigate the influence of biofilm structure on the biofilm removal capacity of endodontic irrigants and to study changes in the architecture of the remaining biofilms. METHODOLOGY:Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were cocultured under different growth conditions on saliva-coated hy...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13027
更新日期:2019-04-01 00:00:00
abstract:AIM:To introduce and assess a novel method for measuring washout resistance of cement-based dental materials, including mineral trioxide aggregate (MTA), to qualitatively verify the results with a clinical simulation and to evaluate the washout resistance of a new root-end filling material. METHODOLOGY:A method for as...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02108.x
更新日期:2013-02-01 00:00:00
abstract:AIM:To evaluate the therapeutic effect of Simvastatin on apical periodontitis in rats by assessing the osteoblast production of receptor activator of nuclear factor-Kappa B Ligand (RANKL) and Osteoprotegerin (OPG) cytokines that are essential for bone resorption. METHODOLOGY:Twenty-five healthy 8- to 10-week-old male ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12101
更新日期:2013-11-01 00:00:00
abstract::This study was conducted to evaluate the efficacy of the antibacterial activity of Ca(OH)2 and a sustained-release device containing chlorhexidine (SRD) in both sterilization and prevention of secondary infection of the root canal system. Bovine root dentine specimens previously incubated with Streptococcus faecalis w...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb00944.x
更新日期:1992-01-01 00:00:00
abstract:AIM:To conduct three-dimensional micro-CT analysis of bone destruction, periapical sclerotic changes and inflammatory root resorption (IRR) to compare the differences between Enterococcus faecalis (Ef)- and Porphyromonas gingivalis (Pg)-induced chronic apical periodontitis (CAP). METHODOLOGY:Mono-species bacteria-indu...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13095
更新日期:2019-07-01 00:00:00
abstract:AIM:To present a tactile method for working length determination in teeth with open apices and to determine its accuracy and repeatability. METHODOLOGY:Ninety teeth with 129 root canals were prepared to create open apices. The correct working length (CWL) for each canal was determined by introducing a file into the ro...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01636.x
更新日期:2009-12-01 00:00:00
abstract::The Netherlands Society for Endodontology has developed a 5-year plan for continuing education that served as a guideline for two basic courses in endodontology. The 2-day basic course has been attended by over 2000 dentists, and has been very much appreciated according to the evaluation outlined in this article. The ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1991.tb00131.x
更新日期:1991-07-01 00:00:00
abstract:AIM:To investigate a potential cause-effect relationship between dentinal microcracks and fracture resistance of mandibular incisors that had not been endodontically treated. METHODOLOGY:Sixty mandibular incisors with circular-shaped canals were selected based on micro-computed tomographic scans to create a homogeneou...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13370
更新日期:2020-11-01 00:00:00
abstract:AIM:This study evaluated the effects of different root canal sealers on the bond strength of a fibreglass post cemented with self-adhesive resin cements. METHODOLOGY:The root canals of 50 extracted maxillary single-rooted canine teeth were prepared with the crown-down technique and randomly divided into five groups ac...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01831.x
更新日期:2011-04-01 00:00:00
abstract:AIM:To test the chemical-physical properties and apatite-forming ability of experimental fluoride-doped calcium silicate cements designed to create novel bioactive materials for use in endodontics and oral surgery. METHODOLOGY:A thermally treated calcium silicate cement (wTC) containing CaCl(2) 5%wt was modified by ad...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01907.x
更新日期:2011-10-01 00:00:00
abstract:AIM:To investigate in vitro the cytocompatibility of the calcium silicate-containing endodontic sealers MTA Fillapex and TotalFill BC Sealer on human periodontal ligament stem cells (hPDLSCs) by assaying their biological responses and compare them with that observed when using an epoxy resin-based sealer (AH Plus). ME...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12596
更新日期:2017-01-01 00:00:00
abstract:AIM:To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. METHODOLOGY:A total of 50 human periodontal ligament samples were obtained from health...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12952
更新日期:2018-12-01 00:00:00
abstract:AIM:This randomized clinical trial evaluated clinical sign/symptoms as well as histological pulp reactions in terms of inflammation and mineralized bridge formation after partial pulpotomy of sound human premolars and placement of a bioceramic paste (iRoot BP) or tooth-colored ProRoot MTA as pulp-covering biomaterials....
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/iej.12231
更新日期:2014-09-01 00:00:00
abstract:AIM:To evaluate the wear of root wall surfaces, the bond strength of sealers to dentine and the demineralization around root filling materials after canals were exposed to acid challenge in situ. METHODOLOGY:Eighty-seven roots of mandibular incisors were selected. Thirty-two were used in the laboratory bond strength s...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12858
更新日期:2018-03-01 00:00:00
abstract:AIM:To compare the penetration resistance (PR) of six thermoplastic gutta-percha (GP) materials against needle penetration at different temperatures. METHODOLOGY:The PR of the following materials based on warm gutta-percha techniques, Beefill GP Cartridge (Beefill), GP Obturator-Hard/Medium/Soft (Dia-H, Dia-M, Dia-S),...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12349
更新日期:2015-06-01 00:00:00
abstract:AIM:To evaluate the fracture resistance and fracture patterns of root filled maxillary first premolar teeth (with mesio-occlusal cavity preparation) restored with several composite restoration designs. METHODOLOGY:One hundred extracted sound human maxillary first premolars were randomly divided into five groups. Group...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2011.02005.x
更新日期:2012-06-01 00:00:00
abstract:AIM:To develop an inexpensive simulation model for training of revitalization procedures. METHODOLOGY:A replica of an immature maxillary central incisor was equipped with a mock blood reservoir at the root apex and embedded in a plaster model. Mock blood consisted of water supplemented with red pigments and fibrinogen...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12765
更新日期:2018-05-01 00:00:00
abstract:AIM:The aim of this in vitro study was to evaluate the shear bond strength of commercially available and experimental glass ionomer cement (GIC) sealers to dentine exposed to common endodontic irrigants. METHODOLOGY:The enamel of 90 bovine incisor crowns, randomly divided into nine equal groups, was ground to expose t...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00238.x
更新日期:1999-11-01 00:00:00
abstract:AIM:To evaluate the presence of bacteria in permanent teeth with intact crowns (without caries, periodontal disease or dental trauma) in patients with sickle cell anaemia (HbSS genotype) by analysing their clinical, imaging and microbiological parameters. METHODOLOGY:This is a case series study nested in a cohort. In ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13476
更新日期:2021-01-12 00:00:00
abstract:AIM:To assess if a contrast medium improved diagnostic yield of endodontic radiographs. METHODOLOGY:Forty-five extracted mandibular premolar teeth were radiographed in bucco-lingual and mesio-distal planes. Access cavities were prepared, pulpal tissue extirpated and Ultravist contrast medium introduced under pressure....
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00872.x
更新日期:2004-12-01 00:00:00
abstract:AIM:To compare the fracture resistance of thin-walled roots after restoration with different types of post systems. METHODOLOGY:One hundred and sixty-five decoronated maxillary central incisors were root filled and randomly assigned to three groups with respect to the remaining dentine thickness of root (1.0 mm, 1.5 m...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01609.x
更新日期:2009-11-01 00:00:00
abstract:AIM:The purpose was to present the frequency of periapical healing in first and second/third mandibular molars, after root-end resections sealed with a dentine-bonded resin composite. METHODOLOGY:Root-end sealing of resected mandibular molar roots was made with dentine-bonded composite (Gluma-Retroplast) as a cover on...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2001.00383.x
更新日期:2001-06-01 00:00:00
abstract:AIM:To evaluate the influence of delayed scanning on images obtained with two PSPs digital systems and on the diagnostic accuracy of vertical root fracture (VRF) by means of objective and subjective analyses. METHODOLOGY:Forty single-rooted human teeth were divided into two groups, one without VRFs and another with VR...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12547
更新日期:2016-10-01 00:00:00
abstract:AIM:To assess the effect of acid-etching duration time on the compressive strength, microstructure and shear bond strength of All-Bond Universal adhesive to MTA-Angelus (AMTA), and Biodentine (BD). METHODOLOGY:Forty-eight cylindrical specimens (4 mm in diameter, 6 mm in height) of AMTA in four groups and 48 specimens ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13026
更新日期:2019-04-01 00:00:00
abstract::Microorganisms and their by-products are considered to be the major cause of pulp and periradicular pathosis. Hence, a major objective in root canal treatment is to disinfect the entire root canal system, which requires that all contents of the root canal system be eliminated as possible sources of infection. This goa...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2591.2008.01540.x
更新日期:2009-04-01 00:00:00
abstract:AIM:The purpose of this study was to determine the cytotoxicity of three different types of root canal sealer on human periodontal ligament (PDL) cells and a permanent hamster cell line (V79 cells). METHODOLOGY:Set specimens from two resin based sealers (AH26 and AHPlus), three zinc oxide-eugenol-based sealers (Canals...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00459.x
更新日期:2002-02-01 00:00:00
abstract::The root canal anatomy of 19 mandibular second molars with C-shaped canals was investigated by rendering the roots transparent and allowing the canal system to be observed by black ink infiltration. The presence of three root canals was most frequent, and lateral canals were found in all roots. Transverse anastomoses ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1990.tb00801.x
更新日期:1990-01-01 00:00:00
abstract:AIM:To investigate amongst Greek endodontists the prevalence and characteristics of musculoskeletal disorders (MSDs) in the past 12 months, the treatment followed, their postures during practice and the adoption of ergonomic standards. METHODOLOGY:One hundred and forty-seven endodontists met the inclusion criteria and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12219
更新日期:2014-08-01 00:00:00
abstract::The effect on coronal leakage of the use of either a single gutta-percha cone or cold lateral condensation of gutta-percha, with a glass ionomer sealer cement was studied. In addition, the effect of the removal of the smear layer was also determined. Eighty-eight single-rooted teeth were prepared chemomechanically usi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00255.x
更新日期:1994-07-01 00:00:00