Abstract:
AIM:The purpose of this study was to determine the cytotoxicity of three different types of root canal sealer on human periodontal ligament (PDL) cells and a permanent hamster cell line (V79 cells). METHODOLOGY:Set specimens from two resin based sealers (AH26 and AHPlus), three zinc oxide-eugenol-based sealers (Canals, Endomethasone and N2) and one calcium hydroxide-based sealer (Sealapex) were eluted with culture medium for 1, 2, 3 and 7 days. Cytotoxicity was judged using tetrazolium bromide reduction assay on human primary PDL cells and V79 cells derived from a Chinese hamster. RESULTS:The results showed that elutes from resin-based, zinc oxide-eugenol-based, and calcium hydroxide-based sealers were cytotoxic to primary human PDL cultures and V79 cells. Calcium hydroxide-based sealer was the least toxic sealer amongst the chemicals tested in both cultures. The cytotoxic response decreased in an order of N2 > Endomethasone > AH26 > AHplus > Canals > Sealapex. CONCLUSIONS:The sensitivity of toxicity depended on the materials tested and the cell culture system used. Thus, the use of both permanent and primary cells is recommended for screening of the cytotoxic effects of root canal sealers. In addition, the results confirmed that root canal sealers constantly dissolve when exposed to an aqueous environment for extended periods, possibly causing moderate or severe cytotoxic reactions. Use of calcium hydroxide-based material as a root canal sealer initially may result in a more favourable response to periradicular tissues.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Huang FM,Tai KW,Chou MY,Chang YCdoi
10.1046/j.1365-2591.2002.00459.xkeywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
153-8issue
2eissn
0143-2885issn
1365-2591pii
459journal_volume
35pub_type
杂志文章abstract:AIM:To evaluate the response of the apical and periapical tissues of dog teeth with apical periodontitis after one-session root canal treatment with and without antimicrobial photodynamic therapy (aPDT) compared with the use of an intracanal dressing. METHODOLOGY:Sixty root canals with an induced periapical lesion wer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12486
更新日期:2016-06-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00755.x
更新日期:2004-06-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12244
更新日期:2014-11-01 00:00:00
abstract:LITERATURE REVIEW:Inter-appointment flare-up is characterized by the development of pain, swelling or both, following endodontic intervention. The causative factors of flare-ups encompass mechanical, chemical and/or microbial injury to the pulp or periradicular tissues. Of these factors, microorganisms are arguably the...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00671.x
更新日期:2003-07-01 00:00:00
abstract::In an effort to simulate the infected dental root canal, bacteria were enclosed in polyethylene tubes that were subcutaneously implanted into rats. Four different bacterial combinations, selected from nine oral species, were tested. The contents of 144 inoculated and implanted 'root canals' were analysed after 2 and 4...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1989.tb00931.x
更新日期:1989-11-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00845.x
更新日期:2004-09-01 00:00:00
abstract:AIM:To identify the number of roots and canal configurations in permanent first molars of the indigenous Kuwaiti sub-population and compare them against a similar group of non-Kuwaiti population in different age groups and gender, and to determine the frequency of a second canal in the mesiobuccal root of maxillary fir...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01427.x
更新日期:2008-09-01 00:00:00
abstract:AIM:To compare the efficacy of reciprocating and rotary techniques with that of hand files for removing gutta-percha and sealer from root canals. METHODOLOGY:The root canals of fifty-four human extracted maxillary central incisors were cleaned and shaped using a crown-down technique to a size 40 and filled with gutta-...
journal_title:International endodontic journal
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更新日期:2013-10-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.01012.x
更新日期:2005-10-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12001
更新日期:2013-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/iej.12294
更新日期:2015-02-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
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更新日期:1989-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01089.x
更新日期:2006-06-01 00:00:00
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更新日期:2021-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-08-01 00:00:00
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更新日期:2004-02-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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更新日期:2014-06-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01883.x
更新日期:2011-08-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12765
更新日期:2018-05-01 00:00:00
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journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13296
更新日期:2020-07-01 00:00:00
abstract:AIM:To evaluate clinically the antibacterial effects of root canal treatment procedures using molecular microbiology analyses. METHODOLOGY:Samples were taken from 14 necrotic root canals of teeth with apical periodontitis before (S1) and after instrumentation with NaOCl irrigation (S2), a final rinse with chlorhexidin...
journal_title:International endodontic journal
pub_type: 杂志文章
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更新日期:2013-03-01 00:00:00