Abstract:
AIM:The objectives of this laboratory-based study were to investigate the effects of GH12 on Enterococcus faecalis biofilm and virulence. METHODOLOGY:Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of GH12 against E. faecalis were first determined. A time-kill assay was further conducted. The effects of GH12 on the expression of virulence and stress genes in E. faecalis were evaluated by RT-qPCR. Crystal violet stain was used to investigate the effects of GH12 on E. faecalis biofilm formation and 1-day-old biofilm. Finally, an ex vivo tooth model contaminated with E. faecalis was used to evaluate the antimicrobial activity of GH12 as an irrigant by CFU counting, SEM and CLSM. One-way anova and Tukey's multiple comparisons test were used to compare the differences amongst groups (α = 0.05). RESULTS:The MICs and MBCs of GH12 against E. faecalis were 8.0 ± 0.0 and 16.0 ± 0.0 mg L-1 , respectively, and GH12 at 32.0 mg L-1 reduced the bacterial numbers by more than 99.9% within 1 min. Various virulence genes (efaA, esp and gelE) and stress genes (dnaK, groEL, ctsR and clpPBCEX) in E. faecalis were significantly downregulated by GH12 at sub-MIC levels (P < 0.05). Additionally, both E. faecalis biofilm formation and the biomass of 1-day-old E. faecalis biofilm were significantly reduced by GH12 (P < 0.05). Elimination of E. faecalis in biofilms from root canal walls was achieved through irrigation with 64.0 mg L-1 GH12 for 30 min. CLSM analysis revealed that GH12 at 64.0 mg L-1 was most effective in eliminating bacteria within dentinal tubules (P < 0.05). CONCLUSION:In a laboratory setting, and when used as an irrigant, GH12 suppressed E. faecalis, downregulated specific virulence and stress-associated genes, eliminated intracanal E. faecalis protected by biofilms and killed bacteria in dentinal tubules. These results emphasize the need for preclinical and clinical studies to explore the potential of GH12 as an antimicrobial agent during root canal treatment.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Li Y,Wang Y,Chen X,Jiang W,Jiang X,Zeng Y,Li X,Feng Z,Luo J,Zhang Ldoi
10.1111/iej.13296subject
Has Abstractpub_date
2020-07-01 00:00:00pages
948-961issue
7eissn
0143-2885issn
1365-2591journal_volume
53pub_type
杂志文章abstract:AIM:To assess the success rate of the root-end filling material, Mineral Trioxide Aggregate (MTA). METHODOLOGY:Referred adult patients were recruited using strict entry criteria and randomly allocated to receive MTA or IRM. A standardized surgical technique was employed: the root end was resected perpendicularly and a...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2003.00682.x
更新日期:2003-08-01 00:00:00
abstract:AIM:To investigate amongst Greek endodontists the incidence of percutaneous injuries, the circumstances associated with them, the therapeutic measures taken after the injuries and their compliance with infection control measures. METHODOLOGY:One hundred and forty-seven endodontists met the inclusion criteria and were ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02126.x
更新日期:2013-03-01 00:00:00
abstract:AIM:To compare the fracture resistance of roots filled with different materials. METHODOLOGY:Thirty-four freshly extracted human maxillary central incisor teeth with similar dimension were selected. Crowns were sectioned at the cemento-enamel junction, and the length of the roots were adjusted to 13 mm. Following the ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01177.x
更新日期:2007-01-01 00:00:00
abstract:AIM:To compare canal and isthmus debris debridement efficacies of the manual dynamic irrigation (MDI) and apical negative pressure (ANP) techniques in the mesial root of mandibular first molars with narrow isthmi, using a closed canal design. METHODOLOGY:Micro-computed tomography was employed to select 20 teeth, each ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01778.x
更新日期:2010-12-01 00:00:00
abstract:AIM:To evaluate the effect of capsaicin on substance P (SP) expression during induced inflammation in rat pulp tissue. METHODOLOGY:Radioimmunoanalysis was used to measure SP levels in 36 mandibular molar pulps taken from six Wistar rats. Twelve samples were obtained from healthy pulps and used as negative control grou...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00893.x
更新日期:2005-01-01 00:00:00
abstract:AIM:To evaluate the percentage volume of voids within cement layers, to determine the push-out bond strength of circular and oval fibre posts luted with different commercial resin cements in oval cross-sectional root canals, and to correlate push-out bond strength values and volume of voids of circular and oval fibre p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12568
更新日期:2016-12-01 00:00:00
abstract:AIM:This randomized, double-blinded, clinical trial evaluated the effect of oral premedication of piroxicam, prednisolone, dexamethasone or placebo on postoperative pain after single-visit root canal treatment in teeth with symptomatic irreversible pulpitis and symptomatic apical periodontitis. METHODOLOGY:The trial i...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/iej.13416
更新日期:2021-02-01 00:00:00
abstract:AIM:The aim of this study was to determine the ability of the Quantec Series 2000 rotary nickel-titanium endodontic system to remove dentinal debris and smear layer produced during canal preparation. METHODOLOGY:A first group (control) of 12 curved root canals was prepared using conventional manual instruments and the...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00231.x
更新日期:1999-05-01 00:00:00
abstract:AIM:To examine the occurrence of Candida spp. in refractory periapical granulomas. METHODOLOGY:One hundred and three surgically removed periapical granulomas were subjected to molecular analysis for the occurrence of Candida albicans. DNA was extracted from the samples using a modified phenol/chloroform/isoamyl alcoho...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00707.x
更新日期:2003-09-01 00:00:00
abstract:AIM:To evaluate the frequency of dentinal microcracks after ultrasonic removal of fractured files from the middle third of root canals using micro-computed tomography (micro-CT). METHODOLOGY:Eighteen bilaterally matched pairs of human mandibular incisors extracted for periodontal reasons were included. The matched pai...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12909
更新日期:2018-09-01 00:00:00
abstract:AIM:The purpose of this study was to determine the cytotoxicity of three different types of root canal sealer on human periodontal ligament (PDL) cells and a permanent hamster cell line (V79 cells). METHODOLOGY:Set specimens from two resin based sealers (AH26 and AHPlus), three zinc oxide-eugenol-based sealers (Canals...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00459.x
更新日期:2002-02-01 00:00:00
abstract:AIM:To describe the decision-making process of the general dental practitioner (GDP) underlying the choice of coronal restoration of a root filled tooth. METHODOLOGY:GDPs were strategically selected with respect to gender, age, undergraduate dental school, service affiliation and duration of professional experience. S...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13442
更新日期:2020-11-07 00:00:00
abstract:LITERATURE REVIEW:Inter-appointment flare-up is characterized by the development of pain, swelling or both, following endodontic intervention. The causative factors of flare-ups encompass mechanical, chemical and/or microbial injury to the pulp or periradicular tissues. Of these factors, microorganisms are arguably the...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00671.x
更新日期:2003-07-01 00:00:00
abstract:AIM:To evaluate the intratubular antimicrobial activity of several oxidant and chelating agents associated with or without surfactants in experimentally infected root canals, using confocal laser scanning microscopy. METHODOLOGY:Twenty-four dentine blocks from bovine incisors were contaminated for 5 days with Enteroco...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12863
更新日期:2018-04-01 00:00:00
abstract:AIM:To evaluate the antimicrobial efficacy of Ocimum sanctum, Cinnamomum zeylanicum, Syzygium aromaticum and 3% sodium hypochlorite (NaOCl) against Enterococcus faecalis in planktonic suspension and biofilm phenotypes. METHODOLOGY:The antibacterial efficacy of different concentrations of aqueous ethanolic extracts of ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12058
更新日期:2013-08-01 00:00:00
abstract:AIM:To evaluate the effect of irrigation with 5% sodium hypochlorite (NaOCl) alone and in conjunction with 17% ethylenediaminetetraacetic acid (EDTA) on tooth surface strain (TSS) and to analyse the influence of irrigation time, root morphology and dentine thickness. METHODOLOGY:Thirty-six single-rooted pre-molars wit...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01655.x
更新日期:2010-03-01 00:00:00
abstract:AIM:To determine if a final rinse with EDTA improves the short-term in-tooth retentive strength of adhesively luted fibre-reinforced composite posts. METHODOLOGY:Six different commercial fibre post systems were tested. Post spaces in extracted human anterior teeth were irrigated with either 6% NaOCl or 6% NaOCl follow...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01482.x
更新日期:2008-12-01 00:00:00
abstract:AIM:To compare in vitro the sealing properties of five different dentine adhesive materials (Prime&Bond NT (PBNT); Prompt L-Pop (PLP); Clearfil SE Bond (CSEB); Scotchbond Multi Purpose Plus (SMPP); EBS-Multi (EBSM)) inside the pulp chamber. METHODOLOGY:Seventy-five recently extracted human molar teeth were used. The r...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.0143-2885.2004.00790.x
更新日期:2004-05-01 00:00:00
abstract::Thirty single-rooted teeth were prepared to receive cast posts. After cementation of the post the teeth were divided into three groups. Group I did not receive any treatment, whereas the posts in groups II and III were treated with an ultrasonic device, for 2 min and 5 min, respectively. The force required for post re...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1995.tb00157.x
更新日期:1995-01-01 00:00:00
abstract:AIM:To examine the role of stromal cell-derived factor 1 (SDF-1) signalling during odontogenic differentiation in human dental pulp cells (HDPCs). METHODOLOGY:Human dental pulp cells were treated with differentiation medium, recombinant human SDF-1, neutralizing antibody for SDF-1 or CXCR4, pertussis toxin (PTX) and A...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12182
更新日期:2014-06-01 00:00:00
abstract:AIM:To investigate the prevalence of periradicular periodontitis (PRP) using cone-beam computed tomography (CBCT) scans in a retrospective cross-sectional epidemiological study in a Scottish subpopulation. METHODOLOGY:Of the 319 CBCT scans performed at Dundee Dental Hospital between November 2009 and July 2012, 245 de...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12228
更新日期:2014-09-01 00:00:00
abstract:AIM:The aim of the present study was to investigate the antimicrobial activity of octenidine on Enterococcus faecalis ATCC 29212 in a dentine block model. METHODOLOGY:Fifty-six root segments of extracted human teeth were infected with E. faecalis for 4 weeks. Octenidine-phenoxyethanol gel (1 : 1) was applied for diffe...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01279.x
更新日期:2007-11-01 00:00:00
abstract:AIM:To compare the antibacterial properties and toxicity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate and 0.2% chlorhexidine gluconate plus 0.2% cetrimide (Cetrexidin); Vebas, San Giuliano, Milan, Italy). METHODOLOGY:The antibacterial effects of the irrigants in vitro were examined after 5 min and ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00673.x
更新日期:2003-06-01 00:00:00
abstract:AIM:To investigate the molecular mechanisms of nitric oxide (NO)-induced cytotoxic effect in human gingival fibroblast (HGF) cells. METHODOLOGY:After sodium nitroprusside (SNP), as NO donor, was treated to HGF, viability was measured by MTT assay and apoptosis was determined by TUNEL and DNA fragmentation assay. Mitoc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12314
更新日期:2015-03-01 00:00:00
abstract:AIM:To evaluate the physical and mechanical properties of two cements with a similar chemical composition to mineral trioxide aggregate and to attempt to improve their properties by producing a composite material. METHODOLOGY:Two cement types were used: a mixture of calcium sulpho-aluminate cement and Portland cement ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01435.x
更新日期:2008-10-01 00:00:00
abstract:AIM:To report the hydration mechanism of white mineral trioxide aggregate (White MTA, Dentsply, Tulsa Dental Products, Tulsa, OK, USA). METHODOLOGY:The chemical constitution of white MTA was studied by viewing the powder in polished sections under the scanning electron microscope (SEM). The hydration of both white MTA...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01248.x
更新日期:2007-06-01 00:00:00
abstract:AIM:To assess the failure mechanism of rotary NiTi instruments by chemical, structural and morphological analyses to provide a rational explanation of the effects of surface and bulk treatments on their resistance to fatigue fracture. METHODOLOGY:Thermal treatment (350-500 °C) was performed on electropolished (EP) and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01759.x
更新日期:2010-10-01 00:00:00
abstract:AIM:To assess and compare the resistance to cyclic fatigue, torsional stress, bending and buckling of ProGlider (PG; Dentsply Maillefer, Ballaigues, Switzerland) instruments with PathFile (PF; Dentsply Maillefer) pathfinding nickel-titanium rotary instruments. METHODOLOGY:Size 16, .02 taper PG and PF instruments were ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12386
更新日期:2015-09-01 00:00:00
abstract:AIMS:To investigate and compare views on management of deep caries and the exposed pulp by Endodontic Society members in Ireland (Irish Endodontic Society [IES]) and Italy (Accademia Italiana di Endodonzia [AIE]). Further aims were to investigate the influence of patient-related factors (age, symptoms) and operator-rel...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13418
更新日期:2020-09-24 00:00:00
abstract:AIM:To examine the surfaces of a root tip removed during surgical endodontic treatment for the presence of microorganisms. SUMMARY:The present clinical case illustrates an endodontic retreatment of a maxillary premolar tooth with a fistula and periapical reaction. The case was under treatment for 1 year, during which ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00753.x
更新日期:2004-01-01 00:00:00