Abstract:
AIM:To investigate an immortalized murine odontoblast cell line as a potential alternative for experimental studies on dentinogenesis. METHODOLOGY:The MO6-G3 cell line was investigated morphologically over 3, 7, 11 and 42 days of culture, using histochemical localization of dentine sialoprotein (DSP), alkaline phosphatase (AP), type I collagen and actin filaments, histoenzymatic staining and biochemical investigation of AP and finally, transmission and scanning electron microscopy. RESULTS:Scanning electron micrographs showed elongated cells. Accordingly, a polarized organization of odontoblasts was observed by transmission electron microscopy, identifying distinct subcellular compartments as described in vivo. The secretion apparatus, which includes cisternae of rough endoplasmic reticulum, Golgi apparatus saccules and secretion vesicles and granules, was longitudinally organized in the supranuclear compartment ending distally in the secretory pole. A cellular process was observed. The investigation of the cytoskeleton network revealed that actin microfilaments were organized in parallel stress fibre oriented depending on the longitudinal axis of the cytoplasm. Immunofluorescent labelling showed a continuous expression of type I collagen, DSP and AP. A unipolar distribution characterized intracellular DSP immunoreactivity. Histoenzymology revealed AP active sites increasing from 3 to 11 days albeit with a moderate level of activity comparatively to the in vivo situation in dental cells. CONCLUSION:This cell line MO6-G3 not only showed the criteria of odontoblast phenotype as previously reported but also the characteristic morphodifferentiation pattern of polarized odontoblasts at the cellular level but with an apparent random distribution.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Mesgouez C,Oboeuf M,Mauro N,Colon P,MacDougall M,Machtou P,Sautier JM,Berdal Adoi
10.1111/j.1365-2591.2006.01089.xsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
453-63issue
6eissn
0143-2885issn
1365-2591pii
IEJ1089journal_volume
39pub_type
杂志文章abstract::The assurance of the quality of a service rendered by a member of the dental profession is an essential feature of any system of peer review in dentistry. This document addresses two essential elements: (i) appropriateness of treatment modality and (ii) quality or level of treatment rendered. In revising these guideli...
journal_title:International endodontic journal
pub_type: 共识发展会议,杂志文章,实务指引
doi:10.1111/j.1365-2591.2006.01180.x
更新日期:2006-12-01 00:00:00
abstract::Four hundred and fifty-five extracted mandibular incisor and 340 extracted mandibular premolar teeth were radiographed to assess the incidence of twin canals as visualized on radiographs taken in the mesio-distal direction. The ability to detect the presence of these twin canals by viewing radiographs taken in the sta...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1991.tb00808.x
更新日期:1991-03-01 00:00:00
abstract:AIM:To compare torque and angle of rotation at fracture of new and used K3 rotary instruments. The relation between size of instrument and torque at fracture was also investigated. METHODOLOGY:Nickel-titanium (Ni-Ti).06 K3 rotary instruments were used in a crown-down manner at 300 r.p.m. to prepare canals in resin blo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00732.x
更新日期:2003-11-01 00:00:00
abstract:AIM:To evaluate the influence of root canal form on the sealing ability of two root canal sealers. METHODOLOGY:Twenty radiographically confirmed straight and 20 curved root canals were prepared with a stepback hand filing technique. Root canal aberrations created during preparation were determined by the use of double...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01079.x
更新日期:2006-04-01 00:00:00
abstract::The purpose of this investigation was to study the effects of a Nd:YAG laser on the cut surface of teeth using scanning electron microscopy (SEM). Eighteen single-rooted teeth were cleaned, shaped, and obturated with gutta-percha and root canal sealer. The apical 3 mm of each tooth were resected with a diamond fissure...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb00758.x
更新日期:1992-11-01 00:00:00
abstract:AIM:The aim of this study was to determine the shaping ability of Quantec LX nickel-titanium rotary instruments in simulated root canals. METHODOLOGY:Forty simulated canals consisting of four different shapes in terms of angle and position of curvature were prepared with Quantec LX instruments. Sequential still images...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2000.00311.x
更新日期:2000-07-01 00:00:00
abstract::This case report highlights the diagnostic challenge that herpes zoster represents if pain develops in the prodromal stage. A 58-year-old male presented with pain in the left maxilla. The symptoms had lasted for 7 months. The first premolar had been extracted soon after the onset, and the second premolar root filled s...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00605.x
更新日期:2002-12-01 00:00:00
abstract::The effect of temperature of plasticization on the sealing ability of Thermafil obturators was evaluated. Forty-eight canals in roots of extracted human mandibular molar teeth were divided into four groups of 12. The root canals had curvatures of less than 25 degrees, as determined using the Schneider technique. Four ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00227.x
更新日期:1994-01-01 00:00:00
abstract::The use of cone beam computed tomography (CBCT) in the diagnosis and/or management of endodontic problems is increasing and is reflected in the exponential rise in publications on this topic in the last two decades. The aim of this paper is to: (i) Review current literature on the endodontic applications of CBCT; (ii)...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13115
更新日期:2019-08-01 00:00:00
abstract::The short-term tissue responses to two potential root-end filling materials, a light-cured glass ionomer cement (Vitrebond) and a reinforced zinc oxide-eugenol cement (Kalzinol), were compared with that to amalgam using a previously devised experimental model. In 24 premolar teeth of beagle dogs (47 roots), a collecti...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00077.x
更新日期:1997-07-01 00:00:00
abstract:AIM:To identify the number of roots and canal configurations in permanent first molars of the indigenous Kuwaiti sub-population and compare them against a similar group of non-Kuwaiti population in different age groups and gender, and to determine the frequency of a second canal in the mesiobuccal root of maxillary fir...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01427.x
更新日期:2008-09-01 00:00:00
abstract:AIM:To analyse torque and force generated whilst shaping curved canals using rotary instruments. METHODOLOGY:A specially designed computer-controlled testing platform was used to record events during the shaping of straight and curved canals in plastic blocks and in extracted human teeth using ProFile.04 instruments. ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.0143-2885.2001.00494.x
更新日期:2002-04-01 00:00:00
abstract:AIM:To investigate a potential cause-effect relationship between dentinal microcracks and fracture resistance of mandibular incisors that had not been endodontically treated. METHODOLOGY:Sixty mandibular incisors with circular-shaped canals were selected based on micro-computed tomographic scans to create a homogeneou...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13370
更新日期:2020-11-01 00:00:00
abstract:AIM:The Bramante method was used to study how the natural curvature of root canals was modified when using the Hero 642 nickel-titanium rotary system. METHODOLOGY:Twenty-four canals were selected in freshly extracted human mandibular molars. Every root canal had an angle of curvature greater than 20 degrees. Roots wer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2001.00440.x
更新日期:2001-12-01 00:00:00
abstract:AIM:To evaluate the percentage volume of voids within cement layers, to determine the push-out bond strength of circular and oval fibre posts luted with different commercial resin cements in oval cross-sectional root canals, and to correlate push-out bond strength values and volume of voids of circular and oval fibre p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12568
更新日期:2016-12-01 00:00:00
abstract:AIM:The objectives of this laboratory-based study were to investigate the effects of GH12 on Enterococcus faecalis biofilm and virulence. METHODOLOGY:Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of GH12 against E. faecalis were first determined. A time-kill assay was further cond...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13296
更新日期:2020-07-01 00:00:00
abstract:AIM:To evaluate the performance of XP-endo Finisher R instruments when removing root filling remnants from oval-shaped canals using microcomputed tomographic (micro-CT) imaging as the analytical tool. Passive ultrasonic irrigation (PUI) was used as a reference technique for comparison. METHODOLOGY:Twenty mandibular in...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13077
更新日期:2019-06-01 00:00:00
abstract:AIM:The aims of this study were firstly to compare the area of canal occupied by gutta-percha, sealer or voids using the System B heating device with that obtained by a modified vertical condensation technique using the Touch 'n Heat: and secondly to compare the temperature changes at the root canal wall and external r...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00215.x
更新日期:1999-08-01 00:00:00
abstract:AIM:To evaluate the torsional properties of the NiTi glide path reciprocating instruments R-Pilot (VDW, Munich, Germany) and WaveOne Gold Glider (Dentsply Sirona, Ballaigues, Switzerland). METHODOLOGY:Ten R-Pilot (size 12.5, .04 taper) and 10 WaveOne Gold Glider (size 15, .02v taper) instruments were used. The torque ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13068
更新日期:2019-06-01 00:00:00
abstract::Teflon tubes containing freshly mixed, polymerized Hydron root canal filling material, fully set AH26 or Teflon were implanted into the mandible of guinea pigs and assessed histologically at 2 days, 1, 2, 4, 12 and 26 weeks. None of the materials tested elicited signs of overt or significant tissue damage, and polymer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1992.tb00749.x
更新日期:1992-07-01 00:00:00
abstract:AIM:To evaluate the bond strength of a resin-based sealer (AH Plus) to root canal dentine after the removal of a zinc oxide-eugenol-based sealer (Endofill), using different retreatment techniques. METHODOLOGY:The root canals of sixty-four maxillary incisors were prepared with ProTaper and filled with Endofill/gutta-pe...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12170
更新日期:2014-05-01 00:00:00
abstract:AIM:To assess the push-out bond strength of two new calcium silicate-based endodontic sealers in the root canals of extracted teeth. METHODOLOGY:Thirty extracted single-rooted central incisors of similar sizes were selected randomly and distributed to three groups (n = 10). All canals were instrumented using ProTaper ...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2011.01925.x
更新日期:2011-12-01 00:00:00
abstract:AIM:To determine the influence of various irrigating solutions on the accuracy of the electronic apex locator facility in the Tri Auto ZX handpiece. METHODOLOGY:One hundred and forty teeth with single canals and mature apices, scheduled for extraction for either periodontal or prosthetic reasons in 76 patients were us...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2007.01234.x
更新日期:2007-05-01 00:00:00
abstract:AIM:To assess if a contrast medium improved diagnostic yield of endodontic radiographs. METHODOLOGY:Forty-five extracted mandibular premolar teeth were radiographed in bucco-lingual and mesio-distal planes. Access cavities were prepared, pulpal tissue extirpated and Ultravist contrast medium introduced under pressure....
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00872.x
更新日期:2004-12-01 00:00:00
abstract::The aim of this systematic review was to address the question: Do different irrigating protocols have an impact on the dislocation resistance of mineral trioxide aggregate (MTA)-based materials? The review was performed using a well-defined search strategy in three databases (PubMed, Scopus, Web of Science) to include...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12898
更新日期:2018-08-01 00:00:00
abstract::Thorough knowledge of anatomical complexities of the root canal system has a direct impact on the effectiveness of canal preparation and filling, and is an essential prerequisite for successful root canal treatment. A wide range of complex variations in root canal anatomy exists, including root canal configuration typ...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12800
更新日期:2018-02-01 00:00:00
abstract:AIM:To evaluate lymphocyte-like cell activation (CD5-positive cells) and the expression of interleukin (IL)-6 and IL-17 in the pulp after tooth bleaching with two concentrations of hydrogen peroxide (H2 O2 ). METHODOLOGY:The right and left maxillary molars from 40 rats were treated randomly with bleaching gel with 20%...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12891
更新日期:2018-07-01 00:00:00
abstract:AIM:To evaluate the resistance to ex vivo bacterial leakage over a 40-day period of root canal fillings with five new root canal sealers: RC Sealer, Epiphany, EndoREZ, GuttaFlow and Acroseal, compared with Apexit, AH Plus and RoekoSeal. METHODOLOGY:One hundred and forty-four single rooted human teeth were divided rand...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01509.x
更新日期:2009-04-01 00:00:00
abstract::This position statement on the management of deep caries and the exposed pulp represents the consensus of an expert committee, convened by the European Society of Endodontology (ESE). Preserving the pulp in a healthy state with sustained vitality, preventing apical periodontitis and developing minimally invasive biolo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13080
更新日期:2019-07-01 00:00:00
abstract:AIM:To compare the ability of cone-beam computed tomography (CBCT) and digital periapical radiographs (PR) to detect simulated tissue-occupied recesses in root canals. METHODOLOGY:A standard canal was created in 30 extracted mandibular premolar roots. Each root was longitudinally split into buccal and lingual halves. ...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2012.02025.x
更新日期:2012-08-01 00:00:00