Abstract:
AIM:To investigate the expression of high-mobility group box 1 (HMGB1) in human dental pulp tissues and the effects of HMGB1 on proliferation and odontoblastic differentiation of human dental pulp cells (hDPCs). METHODOLOGY:Immunohistochemical assay, immunofluorescence staining and flow cytometric analysis were used to detect the expression of HMGB1 in the human dental pulp and hDPCs, respectively. The proliferation of hDPCs was examined by CCK-8 after culturing human primary hDPCs in the presence of HMGB1 with different doses. Odontoblastic differentiation of hDPCs was determined using alkaline phosphatase (ALPase) activity assay and mineralized nodule formation. Important mineralization-related genes such as ALP, dental sialophosphoprotein (DSPP) and dental matrix protein-1 (DMP-1) were determined by real-time polymerase chain reaction. Western blot analysis was performed to determine the difference in expressions of DMP-1 and DSP with or without the presence of exogenous HMGB1. Simultaneously, messenger RNA and protein levels of HMGB1 and RAGE were also detected. The protein level of HMGB1 in the supernatants was quantified using ELISA analysis. RESULTS:HMGB1 was found in human dental pulp tissue and in the nuclei of hDPCs. During hDPC odontoblastic differentiation, HMGB1 translocated from the nuclei to the cytoplasm and then secreted out from hDPCs. Exogenous HMGB1 promoted hDPC proliferation and mineralized nodule formation. It up-regulated the activity of ALPase and the mRNA and protein levels of dentine matrix protein-1 (DMP-1), alkaline phosphatase (ALP), dentine sialophosphoprotein (DSPP) and receptor for advance glycation end (RAGE) of hDPCs. CONCLUSION:HMGB1 promoted the proliferation and odontoblastic differentiation of hDPCs.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Qi SC,Cui C,Yan YH,Sun GH,Zhu SRdoi
10.1111/iej.12112subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1153-63issue
12eissn
0143-2885issn
1365-2591journal_volume
46pub_type
杂志文章abstract:AIM:To evaluate the physicochemical and mechanical properties of Portland cement-based experimental sealers (ES) with different radiopacifying agents (zirconium oxide and niobium oxide micro- and nanoparticles) in comparison with the following conventional sealers: AH Plus, MTA Fillapex and Sealapex. METHODOLOGY:The m...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12167
更新日期:2014-05-01 00:00:00
abstract:AIM:To investigate interfacial stress distribution in restored root filled teeth with various post lengths and crown heights. METHODOLOGY:Three-dimensional mathematical models of a root filled mandibular premolar tooth were constructed. Parts of the tooth structures were replaced with ceramic crowns having three crown...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12397
更新日期:2015-11-01 00:00:00
abstract::Root-canal-filling materials are either placed directly onto vital periapical tissues or may leach through dentine. The tissue response to these materials therefore becomes important and may influence the outcome of endodontic treatment. This paper is a review of the biocompatibility of contemporary orthograde and ret...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00637.x
更新日期:2003-03-01 00:00:00
abstract:AIM:To investigate the prevalence of periradicular periodontitis (PRP) using cone-beam computed tomography (CBCT) scans in a retrospective cross-sectional epidemiological study in a Scottish subpopulation. METHODOLOGY:Of the 319 CBCT scans performed at Dundee Dental Hospital between November 2009 and July 2012, 245 de...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12228
更新日期:2014-09-01 00:00:00
abstract:AIM:To compare in vitro the sealing properties of five different dentine adhesive materials (Prime&Bond NT (PBNT); Prompt L-Pop (PLP); Clearfil SE Bond (CSEB); Scotchbond Multi Purpose Plus (SMPP); EBS-Multi (EBSM)) inside the pulp chamber. METHODOLOGY:Seventy-five recently extracted human molar teeth were used. The r...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.0143-2885.2004.00790.x
更新日期:2004-05-01 00:00:00
abstract::A case is reported of severe loss of alveolar bone in the maxilla, as a result of leakage into the tissues of an arsenical paste from the pulp chamber of an endodontically 'treated' tooth. The history of the use of arsenic in dentistry is discussed, as are the hazards inherent in the use of this outmoded and dangerous...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract:AIM:To assess if a contrast medium improved diagnostic yield of endodontic radiographs. METHODOLOGY:Forty-five extracted mandibular premolar teeth were radiographed in bucco-lingual and mesio-distal planes. Access cavities were prepared, pulpal tissue extirpated and Ultravist contrast medium introduced under pressure....
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00872.x
更新日期:2004-12-01 00:00:00
abstract:AIM:The aim of this study was to determine the shaping ability of Quantec LX nickel-titanium rotary instruments in simulated root canals. METHODOLOGY:Forty simulated canals consisting of four different shapes in terms of angle and position of curvature were prepared with Quantec LX instruments. Sequential still images...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2000.00311.x
更新日期:2000-07-01 00:00:00
abstract:AIM:To determine the cleaning effectiveness and shaping ability of ProTaper and RaCe nickel-titanium rotary instruments during the preparation of curved root canals in extracted human teeth. METHODOLOGY:A total of 48 root canals of mandibular and maxillary molars with curvatures ranging between 25 degrees and 35 degre...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.0143-2885.2004.00783.x
更新日期:2004-04-01 00:00:00
abstract:AIM:To evaluate the effect of a calcium aluminate-based cement (CAC+) on the development of the osteogenic phenotype in vitro. METHODOLOGY:Rat calvaria-derived cells were grown on Thermanox® coverslips for 24 h and then exposed to either samples (4-h set) of CAC+ or mineral trioxide aggregate (MTA) placed on Transwell...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12682
更新日期:2017-08-01 00:00:00
abstract:AIM:To investigate the prevalences of four Treponema species in primary root canal infections using a nested PCR assay. METHODOLOGY:Samples were obtained from 32 infected root canals. Twenty-two cases showed chronic asymptomatic periradicular lesions and 10 symptomatic cases were diagnosed as acute apical periodontiti...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.0143-2885.2003.00607.x
更新日期:2003-01-01 00:00:00
abstract:AIM:To assess and compare the mechanical properties of TRUShape (TRS) with several nickel-titanium rotary instruments. METHODOLOGY:Cyclic fatigue, torsional resistance, flexibility and surface microhardness of TRS (size 25, 0.06v taper), ProTaper Next X2 (PTN X2, size 25, 0.06 taper), ProTaper Gold (PTG F2; size 25, 0...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12700
更新日期:2017-08-01 00:00:00
abstract:AIM:To compare the detectability of residual Epiphany and gutta-percha after root canal retreatment using a dental operating microscope and radiographic examination with the residual area measured after rendering the roots transparent. METHODOLOGY:Sixty extracted single-rooted maxillary central incisor teeth were enla...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2006.01126.x
更新日期:2006-07-01 00:00:00
abstract:AIM:To report clinical complications (pain, necrotic gingival tissue and bone sequestration) resulting from accidental injection of sodium hypochlorite. SUMMARY:Root canal treatment is a routine clinical procedure with few reported complications. Sodium hypochlorite (NaOCl) is commonly used as an irrigant during the p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01340.x
更新日期:2008-03-01 00:00:00
abstract:AIM:To evaluate the resistance to ex vivo bacterial leakage over a 40-day period of root canal fillings with five new root canal sealers: RC Sealer, Epiphany, EndoREZ, GuttaFlow and Acroseal, compared with Apexit, AH Plus and RoekoSeal. METHODOLOGY:One hundred and forty-four single rooted human teeth were divided rand...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01509.x
更新日期:2009-04-01 00:00:00
abstract::The thermal effects of a CO2 laser on the external root surface and inside the root canal were studied in vitro by means of computerized infrared (IR) thermography and a digital thermometer. One-hundred-and-eighty tooth roots with single root canals were irradiated internally and externally with laser power set at 0.5...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00573.x
更新日期:1993-09-01 00:00:00
abstract::The apical seal produced by low-temperature injection of gutta-percha, with or without root canal sealer, in the presence or absence of the smear layer was evaluated quantitatively using an electrochemical technique. The root canals of 44 teeth were prepared with the smear layer intact following a final flush of NaOCl...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00236.x
更新日期:1994-03-01 00:00:00
abstract:AIM:To investigate the reliability of using bovine teeth as an alternative to human teeth in an intra-tooth push-out model by comparing the bond strength of three root canal sealers in both tooth substrates. METHODOLOGY:From human and bovine incisors (12 per group), one 1-mm disc was obtained from each root. On each d...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13085
更新日期:2019-07-01 00:00:00
abstract:AIM:To present a scientometric analysis of the entire body of scientific publications in the field of vital pulp therapy (VPT) and analyse the research trends and popular topics. METHODOLOGY:A comprehensive literature search was conducted in the Web of Science and Scopus databases on 21 August 2020 to identify all art...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13422
更新日期:2021-02-01 00:00:00
abstract::The short-term tissue responses to two potential root-end filling materials, a light-cured glass ionomer cement (Vitrebond) and a reinforced zinc oxide-eugenol cement (Kalzinol), were compared with that to amalgam using a previously devised experimental model. In 24 premolar teeth of beagle dogs (47 roots), a collecti...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00077.x
更新日期:1997-07-01 00:00:00
abstract:AIM:To evaluate the wear of root wall surfaces, the bond strength of sealers to dentine and the demineralization around root filling materials after canals were exposed to acid challenge in situ. METHODOLOGY:Eighty-seven roots of mandibular incisors were selected. Thirty-two were used in the laboratory bond strength s...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12858
更新日期:2018-03-01 00:00:00
abstract:AIM:To evaluate the effect of multiple refreshment/activation cycles and temperature on the reaction rate of sodium hypochlorite (NaOCl) with bovine dentine during ultrasonic activated irrigation (UAI) under laboratory conditions. METHODOLOGY:The root canal walls of 24 standardized root canals in bovine incisors were ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12125
更新日期:2014-02-01 00:00:00
abstract:AIM:To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. METHODOLOGY:A total of 50 human periodontal ligament samples were obtained from health...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12952
更新日期:2018-12-01 00:00:00
abstract:AIM:To evaluate the therapeutic effect of Simvastatin on apical periodontitis in rats by assessing the osteoblast production of receptor activator of nuclear factor-Kappa B Ligand (RANKL) and Osteoprotegerin (OPG) cytokines that are essential for bone resorption. METHODOLOGY:Twenty-five healthy 8- to 10-week-old male ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12101
更新日期:2013-11-01 00:00:00
abstract:AIM:To compare the toxicity of methacrylate resin-based root canal sealers with sealers based on epoxy resin and silicone by two-well established cell culture methods. METHODOLOGY:Specimens of AH Plus, EndoREZ, RoekoSeal and Epiphany were prepared for direct contact in the Millipore filter diffusion test and as extrac...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01321.x
更新日期:2008-01-01 00:00:00
abstract:AIMS:The aims of this study were to investigate the pattern of transmission of laser light through carious human molar teeth, and to examine the effect on light transmission of reactions to caries in the dentine. METHODOLOGY:Sectioned carious human molars were examined whilst illuminated from their external surfaces b...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2001.00428.x
更新日期:2001-10-01 00:00:00
abstract:AIM:To investigate the role of the Ca(2+) -Mg(2+) ion channel TRPM7 in the proliferation, migration and osteogenic differentiation of human dental pulp stem cells (hDPSCs). METHODOLOGY:Immunohistochemistry was used to localize expression of TRPM7 in human dental pulp tissues and in cultured hDPSCs. Isolated hDPSCs wer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12193
更新日期:2014-06-01 00:00:00
abstract:AIM:To characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. METHODOLOGY:Apical lesion samples were collected from 12 patients (23-80 years old) who visited the Kyushu University Hospital for apicectomy with persistent periapical lesions assoc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12361
更新日期:2015-08-01 00:00:00
abstract:AIM:To compare 2D with 3D radiography in assessing the treatment outcome 1 year after periapical surgery. METHODOLOGY:In this prospective study, periapical radiographs (PA) and cone beam computed tomography (CBCT) were performed 1 year after periapical surgery. Three calibrated observers independently evaluated the ra...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12548
更新日期:2016-10-01 00:00:00
abstract::The objective of this study was to compare the adaptation of mechanically softened gutta-percha to the root canal wall in the presence and absence of smear layer. The root canals of 20 freshly extracted human maxillary incisors were cleaned and shaped. Prior to obturation, 10 root canals were irrigated with 20 ml of 5...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1995.tb00312.x
更新日期:1995-09-01 00:00:00