Abstract:
AIM:To investigate the prevalences of four Treponema species in primary root canal infections using a nested PCR assay. METHODOLOGY:Samples were obtained from 32 infected root canals. Twenty-two cases showed chronic asymptomatic periradicular lesions and 10 symptomatic cases were diagnosed as acute apical periodontitis. DNA extracted from the samples was initially amplified using universal 16S rDNA primers. A second round of amplification used the first PCR products to detect a specific fragment of the 16S rDNA of each Treponema denticola, T. socranskii, T. vincentii and T. pectinovorum. RESULTS:Bacteria were present in all cases sampled. T. denticola was detected in 77.3% of the asymptomatic cases, T. socranskii in 40.9%, T. vincentii in 18.2% and T. pectinovorum in 13.6%. In the cases diagnosed as acute apical periodontitis, T. denticola was detected in 80%, whilst T. socranskii and T. vincentii were detected in 40% and 10% of cases, respectively. No symptomatic case yielded T. pectinovorum. In general, nested PCR detected T. denticola in 78.1% of the cases, T. socranskii in 40.6%, T. vincentii in 15.6% and T. pectinovorum in 9.4%. At least one of the four Treponema species was found in 84.4% of the cases examined. CONCLUSIONS:The species T. denticola was detected in a large number of the cases examined: the prevalence of T. socranskii was also relatively high. The species T. vincentii and T. pectinovorum were also found, but in a smaller number of cases. Based on these data, the recognized pathogenicity of these microorganisms and their involvement with other oral diseases, they should be included in the restricted set of putative endodontic pathogens.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Rôças IN,Siqueira JF Jr,Andrade AF,Uzeda Mdoi
10.1046/j.0143-2885.2003.00607.xkeywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
20-6issue
1eissn
0143-2885issn
1365-2591journal_volume
36pub_type
杂志文章abstract::A case is reported where the high flow characteristics of thermoplasticized gutta-percha provided an efficient seal even without adequate shaping of an iatrogenically damaged root canal system. Persistent periradicular irritation was reduced significantly, or isolated within the root canal space, thus allowing periapi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1995.tb00169.x
更新日期:1995-03-01 00:00:00
abstract::The use of cone beam computed tomography (CBCT) in the diagnosis and/or management of endodontic problems is increasing and is reflected in the exponential rise in publications on this topic in the last two decades. The aim of this paper is to: (i) Review current literature on the endodontic applications of CBCT; (ii)...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13115
更新日期:2019-08-01 00:00:00
abstract:AIM:The aim of this study was to test the null hypothesis that sodium hypochlorite irrigation of root canals does not alter the properties of dentine and contribute to the weakening of root-treated teeth. METHODOLOGY:The effect of two concentrations (0.5%, 5.25%) of sodium hypochlorite (NaOCl) and saline on (i) the el...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2001.00357.x
更新日期:2001-03-01 00:00:00
abstract:AIM:To develop and preliminarily evaluate a new screening instrument for atypical odontalgia (AO) or persistent dentoalveolar pain disorder (PDAP). To evaluate the instrument's performance in detecting AO/PDAP amongst a heterogeneous group of orofacial pain conditions and pain-free controls and empirically compare its ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13017
更新日期:2019-03-01 00:00:00
abstract:AIM:To evaluate the cytotoxicity and the mechanism of cell aggression of peracetic acid (PA) in comparison with sodium hypochlorite (NaOCl). METHODOLOGY:L929 fibroblasts were exposed to 1% PA and 2.5% NaOCl, at several dilutions for 10 min. The following parameters were evaluated: cell metabolism by methylthiazol tetr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12750
更新日期:2018-05-01 00:00:00
abstract:AIM:To elucidate mechanisms by which mineral trioxide aggregate (MTA) suppresses pro-inflammatory cytokine mRNA expression in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. METHODOLOGY:Mineral trioxide aggregate extracts were prepared by immersing set ProRoot MTA in culture medium. RAW264.7 cells were cultu...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13386
更新日期:2020-12-01 00:00:00
abstract:AIM:To develop an inexpensive simulation model for training of revitalization procedures. METHODOLOGY:A replica of an immature maxillary central incisor was equipped with a mock blood reservoir at the root apex and embedded in a plaster model. Mock blood consisted of water supplemented with red pigments and fibrinogen...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12765
更新日期:2018-05-01 00:00:00
abstract:AIM:To develop a convenient method for the localization and quantification of live and dead bacteria in human ex vivo mineralized dentinal tubules. METHODOLOGY:The roots from human single-rooted teeth (n = 12) were infected with Enterococcus faecalis V583 and either treated with calcium hydroxide paste or left untreat...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01867.x
更新日期:2011-07-01 00:00:00
abstract:AIM:To evaluate, using an experimental immature tooth model, the fracture resistance of bovine incisors submitted to different reinforcement treatments with mineral trioxide aggregate (MTA). METHODOLOGY:An immature tooth model was created by sectioning the coronal and apical portions of 40 bovine incisors 8 mm above a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01266.x
更新日期:2007-09-01 00:00:00
abstract:AIM:To elucidate the expressions of MMP-8 and MMP-13 in experimentally induced rat periradicular lesions by means of the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining. METHODOLOGY:Thirty rats were used and periradicular lesions in mandibular first molar teeth were establishe...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01880.x
更新日期:2011-08-01 00:00:00
abstract:AIM:The aim of this study was to determine the shaping ability of GT Rotary Files in simulated root canals. METHODOLOGY:Forty canals with four different shapes in terms of angle (40 degrees and 60 degrees) and position of curvature (straight section before curve: 8 and 12 mm) were prepared using a crown-down/stepback ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00540.x
更新日期:2002-07-01 00:00:00
abstract:AIM:To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infections and apical periodontitis, and to assess the influence of calcium hydroxide medication [Ca(OH)2 ], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and R...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13403
更新日期:2021-01-01 00:00:00
abstract:AIM:The purpose of this study was to evaluate clinically and radiographically pulpotomies carried out under intrapulpal injection of anaesthetic solution. METHODOLOGY:Forty-one permanent mandibular molar teeth presenting with deep carious lesions and/or exposed pulps, with or without periapical changes on radiographic...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2001.00414.x
更新日期:2001-09-01 00:00:00
abstract:AIM:To develop a precise and simple mounting model (MM) for evaluating apex locators and to compare the repeatability of the MM with a conventional visual method (VM). METHODOLOGY:Electronic working length determination was performed in 32 maxillary central incisors using two methods: (i) the MM method and (ii) a conv...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01052.x
更新日期:2006-02-01 00:00:00
abstract:AIM:To evaluate the bond strength of self-adhesive resin cements (SARCs) to glass fibre posts with or without a silane coupling agent. METHODOLOGY:The SARCs tested were: Maxcem Elite (MXE; Kerr), RelyX Unicem clicker (UNI; 3M ESPE), seT capsule (SET; SDI), and SmartCem 2 (SC2; Dentsply Caulk). The conventional cement ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01883.x
更新日期:2011-08-01 00:00:00
abstract:AIM:To investigate differences in the root canal morphology of permanent mandibular molar teeth at various ages. METHODOLOGY:Four hundred and eighty permanent mandibular first and second molars were examined. First and second molars were divided into six and five groups, respectively, according to the age of the patie...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01428.x
更新日期:2008-10-01 00:00:00
abstract:AIM:To evaluate the performance of XP-endo Finisher R instruments when removing root filling remnants from oval-shaped canals using microcomputed tomographic (micro-CT) imaging as the analytical tool. Passive ultrasonic irrigation (PUI) was used as a reference technique for comparison. METHODOLOGY:Twenty mandibular in...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13077
更新日期:2019-06-01 00:00:00
abstract::Sodium hypochlorite (NaOCl), is the most popular irrigation solution used in root canal treatment but it is known to be an irritant to vital tissues. There have been many case reports dealing with toxic effects of this material. Although the allergic potential of NaOCl has been described in the medical literature, onl...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00247.x
更新日期:1994-05-01 00:00:00
abstract:AIM:The purpose of this study was to evaluate the influence of rotational speed, torque, and operator experience with a specific Ni-Ti rotary instrumentation technique on the incidence of locking, deformation and separation of instruments. METHODOLOGY:ProFile Ni-Ti rotary instruments (PRI) sizes 40-15 with a 6% taper ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2001.00352.x
更新日期:2001-01-01 00:00:00
abstract:AIM:To determine the bacterial microbiota in root canals associated with persistent apical periodontitis and their relationship with the clinical characteristics of patients using next-generation sequencing (NGS). METHODOLOGY:Bacterial samples from root canals associated with teeth having persistent apical periodontit...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12953
更新日期:2018-12-01 00:00:00
abstract:AIM:To investigate the role of the Ca(2+) -Mg(2+) ion channel TRPM7 in the proliferation, migration and osteogenic differentiation of human dental pulp stem cells (hDPSCs). METHODOLOGY:Immunohistochemistry was used to localize expression of TRPM7 in human dental pulp tissues and in cultured hDPSCs. Isolated hDPSCs wer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12193
更新日期:2014-06-01 00:00:00
abstract::Thorough knowledge of anatomical complexities of the root canal system has a direct impact on the effectiveness of canal preparation and filling, and is an essential prerequisite for successful root canal treatment. A wide range of complex variations in root canal anatomy exists, including root canal configuration typ...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12800
更新日期:2018-02-01 00:00:00
abstract:AIM:To analyse responses from dental practitioners (DPs) on how secure they felt as a newly graduated dentist, level of confidence or self-efficacy when performing root canal treatment (RCT), and if undergraduate (UG) education in Endodontics adequately met their needs in a dental practice. METHODOLOGY:An electronic q...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13455
更新日期:2020-11-30 00:00:00
abstract:AIM:To evaluate ex vivo the incidence of defects in root dentine before and after root canal preparation and filling. METHODOLOGY:Eighty extracted mandibular premolars were divided equally in four groups. Group 1 was left unprepared. All other root canals were prepared using Gates Glidden drills and System GT files up...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01502.x
更新日期:2009-03-01 00:00:00
abstract:AIM:To investigate the prevalence of periradicular periodontitis (PRP) using cone-beam computed tomography (CBCT) scans in a retrospective cross-sectional epidemiological study in a Scottish subpopulation. METHODOLOGY:Of the 319 CBCT scans performed at Dundee Dental Hospital between November 2009 and July 2012, 245 de...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12228
更新日期:2014-09-01 00:00:00
abstract:AIM:Apatite-forming ability, considered as an index of bioactivity (bond-to-bone ability), was tested on ProRoot MTA cement after immersion in phosphate-containing solution (DPBS). METHODOLOGY:Disk samples were prepared and immersed in DPBS for 10 min, 5 h, 1 and 7 days. The cement surface was studied by attenuated to...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01768.x
更新日期:2010-10-01 00:00:00
abstract::During the past few decades several authors have perpetuated the notion that nearly half of all periapical lesions are radicular cysts. A few studies, based on meticulous serial sectioning of periapical lesions retrieved in toto, have shown that the actual incidence of radicular cyst is only about 15% of all periapica...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.1998.00146.x
更新日期:1998-05-01 00:00:00
abstract:AIM:To evaluate the frequency of dentinal microcracks after ultrasonic removal of fractured files from the middle third of root canals using micro-computed tomography (micro-CT). METHODOLOGY:Eighteen bilaterally matched pairs of human mandibular incisors extracted for periodontal reasons were included. The matched pai...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12909
更新日期:2018-09-01 00:00:00
abstract::The aim of designing and fabricating the surgical templates was to assist the surgical component of premolar transplantation, by establishing and replicating the root dimensions of the donor premolar tooth. The correct template could be used to assess the socket preparation (width and depth) prior to placement of the ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02077.x
更新日期:2012-11-01 00:00:00
abstract::The assurance of the quality of a service rendered by a member of the dental profession is an essential feature of any system of peer review in dentistry. This document addresses two essential elements: (i) appropriateness of treatment modality and (ii) quality or level of treatment rendered. In revising these guideli...
journal_title:International endodontic journal
pub_type: 共识发展会议,杂志文章,实务指引
doi:10.1111/j.1365-2591.2006.01180.x
更新日期:2006-12-01 00:00:00