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Quality control of overexpressed membrane proteins.

Abstract：

:Overexpression of membrane proteins in Escherichia coli frequently leads to the formation of aggregates or inclusion bodies, which is undesirable for most studies. Ideally, one would like to optimize the expression conditions by monitoring simultaneously and rapidly both the amounts of properly folded and aggregated membrane protein, a requirement not met by any of the currently available methods. Here, we describe a simple gel-based approach with green fluorescent protein as folding indicator to detect well folded and aggregated proteins simultaneously. The method allows for rapid screening and, importantly, pinpointing the most likely bottlenecks in protein production.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Geertsma ER,Groeneveld M,Slotboom DJ,Poolman B

doi

10.1073/pnas.0802190105

subject

Has Abstract

pub_date

2008-04-15 00:00:00

pages

5722-7

issue

15

eissn

0027-8424

issn

1091-6490

pii

0802190105

journal_volume

105

pub_type

杂志文章

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