Peptide-in-groove interactions link target proteins to the beta-propeller of clathrin.

abstract：:Crithidia fasciculata cells were treated with a plasmid (pDK96) containing pBR322 sequences, a Leishmania tarentolae maxicircle autonomously replicating sequence, and the bacterial gene for aminoglycoside 3' phosphotransferase I inserted between the yeast alcohol dehydrogenase 1 promotor and terminator sequences. Resi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes DE,Simpson L

更新日期：1986-08-01 00:00:00

abstract：:Saccharomyces cerevisiae is one of the most important model organisms and has been a valuable asset to human civilization. However, despite its extensive use in the last 9,000 y, the existence of a seasonal cycle outside human-made environments has not yet been described. We demonstrate the role of social wasps as vec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stefanini I,Dapporto L,Legras JL,Calabretta A,Di Paola M,De Filippo C,Viola R,Capretti P,Polsinelli M,Turillazzi S,Cavalieri D

更新日期：2012-08-14 00:00:00

abstract：:Both polarities of the satellite RNA of tobacco ringspot virus are sources of self-cleaving sequences. RNA of the less abundant, negative polarity, designated sTobRV-(-)RNA, has cleaving activity that was mapped previously to two noncontiguous regions of the polyribonucleotide chain. Endoribonucleolytic oligoribonucle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldstein PA,Buzayan JM,van Tol H,deBear J,Gough GR,Gilham PT,Bruening G

更新日期：1990-04-01 00:00:00

abstract：:Fungi that are resistant or sensitive to the singlet oxygen-generating toxin cercosporin were assayed for their ability to detoxify it by reduction. Cercosporin reduction was assayed microscopically by using bandpass filters to differentiate between fluorescence emission from cercosporin and reduced cercosporin. Hypha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daub ME,Leisman GB,Clark RA,Bowden EF

更新日期：1992-10-15 00:00:00

abstract：:Ecosystem restoration aims to restore biodiversity and valuable functions that have been degraded or lost. The Coral Triangle is a hotspot for marine biodiversity held in its coral reefs, seagrass meadows, and mangrove forests, all of which are in global decline. These coastal ecosystems support valuable fisheries and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams SL,Ambo-Rappe R,Sur C,Abbott JM,Limbong SR

更新日期：2017-11-07 00:00:00

abstract：:Contractile forces are essential for many developmental processes involving cell shape change and tissue deformation. Recent experiments on reconstituted actomyosin networks, the major component of the contractile machinery, have shown that active contractility occurs above a threshold motor concentration and within a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang S,Wolynes PG

更新日期：2012-04-24 00:00:00

abstract：:We have evaluated the effect of ligand binding on expression of the receptor for IgE on rat basophilic leukemia (RBL) cells. RBL cells were grown in the presence or absence of 131I-labeled IgE and sometimes were also surface labeled with 125I. We found that cells grown in the presence of IgE continued to accumulate re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furuichi K,Rivera J,Isersky C

更新日期：1985-03-01 00:00:00

abstract：:Cell mechanics plays a role in stem cell reprogramming and differentiation. To understand this process better, we created a genetically encoded optical probe, named actin-cpstFRET-actin (AcpA), to report forces in actin in living cells in real time. We showed that stemness was associated with increased force in actin....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo J,Wang Y,Sachs F,Meng F

更新日期：2014-12-09 00:00:00

abstract：:Fas, a member of the tumor necrosis factor receptor family, can induce apoptosis when activated by Fas ligand binding or anti-Fas antibody crosslinking. Genetic studies have shown that a defect in Fas-mediated apoptosis resulted in abnormal development and function of the immune system in mice. A point mutation in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu K,Niu X,Williams LT

更新日期：1995-12-05 00:00:00

abstract：:Specific protein domains and amino acids responsible for the apparent capacity of P-glycoprotein (mdr) to recognize and transport a large group of structurally unrelated drugs have not been identified. We have introduced a single Ser----Phe substitution within the predicted TM11 domain of mdr1 (position 941) and mdr3 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gros P,Dhir R,Croop J,Talbot F

更新日期：1991-08-15 00:00:00

abstract：:In an investigation to determine how proteins are localized within the nucleus of a cell, we demonstrate that the restriction endonuclease EcoRI is able to enter and function within the nucleus of Saccharomyces cerevisiae when this prokaryotic protein is synthesized in vivo. The EcoRI endonuclease was produced in yeas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnes G,Rine J

更新日期：1985-03-01 00:00:00

abstract：:Homeostasis is indispensable to counteract the destabilizing effects of Hebbian plasticity. Although it is commonly assumed that homeostasis modulates synaptic strength, membrane excitability, and firing rates, its role at the neural circuit and network level is unknown. Here, we identify changes in higher-order netwo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu YK,Hengen KB,Turrigiano GG,Gjorgjieva J

更新日期：2020-09-29 00:00:00

abstract：:Psychedelic drugs have a long history of use in healing ceremonies, but despite renewed interest in their therapeutic potential, we continue to know very little about how they work in the brain. Here we used psilocybin, a classic psychedelic found in magic mushrooms, and a task-free functional MRI (fMRI) protocol desi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carhart-Harris RL,Erritzoe D,Williams T,Stone JM,Reed LJ,Colasanti A,Tyacke RJ,Leech R,Malizia AL,Murphy K,Hobden P,Evans J,Feilding A,Wise RG,Nutt DJ

更新日期：2012-02-07 00:00:00

abstract：:Six overlapping cosmid clones having an average insert size of 40 kilobase pairs were identified and isolated from a human genomic library by using a cDNA probe, Alu-7, specific for the amino acid sequence of C4d, a known region of the fourth component of human complement. Analysis of these genomic clones by restricti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carroll MC,Porter RR

更新日期：1983-01-01 00:00:00

abstract：:The excision and reintegration of transposable elements (TEs) restructure their host genomes, generating cellular diversity involved in evolution, development, and the etiology of human diseases. Our current knowledge of TE behavior primarily results from bulk techniques that generate time and cell ensemble averages, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim NH,Lee G,Sherer NA,Martini KM,Goldenfeld N,Kuhlman TE

更新日期：2016-06-28 00:00:00

abstract：:The avian predentary is a small skeletal structure located rostral to the paired dentaries found only in Mesozoic ornithuromorphs. The evolution and function of this enigmatic element is unknown. Skeletal tissues forming the predentary and the lower jaws in the basal ornithuromorph Yanornis martini are identified usin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bailleul AM,Li Z,O'Connor J,Zhou Z

更新日期：2019-12-03 00:00:00

abstract：:Tumor metastasis is the cause of most cancer deaths. Although metastases can form in multiple end organs, the liver is recognized as a highly permissive organ. Nevertheless, there is evidence for immune cell-mediated mechanisms that function to suppress liver metastasis by certain tumors, although the underlying mecha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kimura Y,Inoue A,Hangai S,Saijo S,Negishi H,Nishio J,Yamasaki S,Iwakura Y,Yanai H,Taniguchi T

更新日期：2016-12-06 00:00:00

abstract：:Despite their small size, some insects, such as crickets, can produce high amplitude mating songs by rubbing their wings together. By exploiting structural resonance for sound radiation, crickets broadcast species-specific songs at a sharply tuned frequency. Such songs enhance the range of signal transmission, contain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mhatre N,Montealegre-Z F,Balakrishnan R,Robert D

更新日期：2012-05-29 00:00:00

abstract：:Phosphatidylinositol 3 (PI3)-kinase enhancer (PIKE) is a nuclear GTPase that enhances PI3-kinase activity in a GTP-dependent manner. Both PIKE-L and -A isoforms contain GTPase, pleckstrin homology (PH), ADP ribosylation factor-GTPase-activating protein, and two ankyrin repeats domains, and C-terminal ADP ribosylation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Y,Liu Z,Ye K

更新日期：2005-11-15 00:00:00

abstract：:In Drosophila, heterochromatin protein 1 (HP1) suppresses the expression of euchromatic genes that are artificially translocated adjacent to heterochromatin by expanding heterochromatin structure into neighboring euchromatin. The purpose of this study was to determine whether HP1 functions as a transcriptional repress...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang KK,Eissenberg JC,Worman HJ

更新日期：2001-09-25 00:00:00

abstract：:The 24 human chromosome types of normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophoto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrano AV,Gray JW,Langlois RG,Burkhart-Schultz KJ,Van Dilla MA

更新日期：1979-03-01 00:00:00

abstract：:The DNA damage response is an essential process for the survival of living cells. In a subset of stress-responsive genes in humans, Elongin controls transcription in response to multiple stimuli, such as DNA damage, oxidative stress, and heat shock. Yeast Elongin (Ela1-Elc1), along with Def1, is known to facilitate ub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Damodaren N,Van Eeuwen T,Zamel J,Lin-Shiao E,Kalisman N,Murakami K

更新日期：2017-12-12 00:00:00

abstract：:Single crystal diamond produced by chemical vapor deposition (CVD) at very high growth rates (up to 150 microm/h) has been successfully annealed without graphitization at temperatures up to 2200 degrees C and pressures <300 torr. Crystals were annealed in a hydrogen environment by using microwave plasma techniques for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meng YF,Yan CS,Lai J,Krasnicki S,Shu H,Yu T,Liang Q,Mao HK,Hemley RJ

更新日期：2008-11-18 00:00:00

abstract：:Accumulation of amyloid-beta (Aβ), which is associated with Alzheimer's disease, can be caused by excess production or insufficient clearance. Because of its β-sheet structure, fibrillar Aβ is resistant to proteolysis, which would contribute to slow degradation of Aβ plaques in vivo. Fibrillar Aβ can be internalized b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Solé-Domènech S,Rojas AV,Maisuradze GG,Scheraga HA,Lobel P,Maxfield FR

更新日期：2018-02-13 00:00:00

abstract：:Establishment of loss-of-function phenotypes is often a key step in determining the biological function of a gene. We describe a procedure to obtain mutant petunia plants in which a specific gene with known sequence is inactivated by the transposable element dTph1. Leaves are collected from batches of 1000 plants with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koes R,Souer E,van Houwelingen A,Mur L,Spelt C,Quattrocchio F,Wing J,Oppedijk B,Ahmed S,Maes T

更新日期：1995-08-29 00:00:00

abstract：:Endothelin, originally purified from porcine aortic endothelial cells, is widely distributed in tissues and is recognized as a product of epithelial cells, glial cells, and neurons in addition to endothelial cells. We found evidence by mRNA content and immunoreactivity that this peptide is synthesized in rat parathyro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujii Y,Moreira JE,Orlando C,Maggi M,Aurbach GD,Brandi ML,Sakaguchi K

更新日期：1991-05-15 00:00:00

abstract：:We have analyzed ligand-receptor complexes resulting from (i) the incubation of canine hepatic plasma membranes with [125I]iodoglucagon and (ii) subsequent gentle solubilization of receptor-bound ligand with digitonin. The complexes (molecular weight approximately equal to 500,000) retain the radiolabeled ligand durin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mason JC,Tager HS

更新日期：1985-10-01 00:00:00

abstract：:Human settlement of Oceania marked the culmination of a global colonization process that began when humans first left Africa at least 90,000 years ago. The precise origins and dispersal routes of the Austronesian peoples and the associated Lapita culture remain contentious, and numerous disparate models of dispersal (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Larson G,Cucchi T,Fujita M,Matisoo-Smith E,Robins J,Anderson A,Rolett B,Spriggs M,Dolman G,Kim TH,Thuy NT,Randi E,Doherty M,Due RA,Bollt R,Djubiantono T,Griffin B,Intoh M,Keane E,Kirch P,Li KT,Morwood M,Pedriñ

更新日期：2007-03-20 00:00:00

abstract：:Most of the activities of IFN-gamma are the result of STAT1-mediated transcriptional responses. In this study, we show that the BRCA1 tumor suppressor acts in concert with STAT1 to differentially activate transcription of a subset of IFN-gamma target genes and mediates growth inhibition by this cytokine. After IFN-gam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ouchi T,Lee SW,Ouchi M,Aaronson SA,Horvath CM

更新日期：2000-05-09 00:00:00

abstract：:XMAP215/Dis1 family proteins positively regulate microtubule growth. Repeats at their N termini, called TOG domains, are important for this function. While TOG domains directly bind tubulin dimers, it is unclear how this interaction translates to polymerase activity. Understanding the functional roles of TOG domains i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Widlund PO,Stear JH,Pozniakovsky A,Zanic M,Reber S,Brouhard GJ,Hyman AA,Howard J

更新日期：2011-02-15 00:00:00