Active contractility in actomyosin networks.

abstract：:Inhibitory gamma-aminobutyric acid-mediated synaptic currents were studied in dissociated primary cultures of neonatal rat cortex with the whole-cell patch-clamp technique. Immunocytochemical staining of the cultures showed the presence of a large number of glutamic acid decarboxylase-containing neurons, and electrica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vicini S,Alho H,Costa E,Mienville JM,Santi MR,Vaccarino FM

更新日期：1986-12-01 00:00:00

abstract：:The pore domain of voltage-gated potassium (Kv) channels consists of transmembrane helices S5 and S6, the turret, the pore helix, the selectivity filter, and the loop preceding S6, with a tertiary reentrant structure between S5 and S6. Using biogenic intermediates, mass tagging (pegylation), and a molecular tape measu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gajewski C,Dagcan A,Roux B,Deutsch C

更新日期：2011-02-22 00:00:00

abstract：:Morphine is one of the most prescribed and effective drugs used for the treatment of acute and chronic pain conditions. In addition to its central effects, morphine can also produce peripheral analgesia. However, the mechanisms underlying this peripheral action of morphine have not yet been fully elucidated. Here, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cunha TM,Roman-Campos D,Lotufo CM,Duarte HL,Souza GR,Verri WA Jr,Funez MI,Dias QM,Schivo IR,Domingues AC,Sachs D,Chiavegatto S,Teixeira MM,Hothersall JS,Cruz JS,Cunha FQ,Ferreira SH

更新日期：2010-03-02 00:00:00

abstract：:We have generated a transgenic mouse line by microinjection of a chimeric DNA fragment (KER-CAT) containing a hair-specific, murine ultra-high-sulfur keratin promoter (KER) fused to the coding region of the bacterial chloramphenicol acetyltransferase (CAT) gene. A 671-base pair (bp) stretch of the 5' promoter region w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McNab AR,Andrus P,Wagner TE,Buhl AE,Waldon DJ,Kawabe TT,Rea TJ,Groppi V,Vogeli G

更新日期：1990-09-01 00:00:00

abstract：:The immunity protein to colicin A (Cai), which is constitutively expressed at a very low level in Escherichia coli strains, has been studied in recombinant plasmid constructs allowing expression of various immunity fusion proteins under the control of inducible promoters. The 13-amino acid NH2-terminal region of Cai w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geli V,Baty D,Lazdunski C

更新日期：1988-02-01 00:00:00

abstract：:Splenic lymphocytes from a Lewis rat, immunized with purified estradiol-receptor complex of calf uterine nuclei, were fused with cells of three different mouse myeloma lines (P3-X63-Ag8, P3-NSI/I-Ag4-1, and Sp2/0-Ag14) to yield hybridoma cultures, 9% of which produced antibodies to the receptor protein (estrophilin). ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greene GL,Fitch FW,Jensen EV

更新日期：1980-01-01 00:00:00

abstract：:An RNA-directed DNA polymerase was isolated from the peripheral blood leukocytes of a patient with acute myelomonocytic leukemia by successive purification of a particulate cytoplasmic fraction with endogenous, ribonuclease-sensitive DNA polymerase activity. Like RNA-directed DNA polymerase from mammalian type-C virus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallagher RE,Todaro GJ,Smith RG,Livingston DM,Gallo RC

更新日期：1974-04-01 00:00:00

abstract：:The current model to explain the organization of the mammalian nervous system is based on studies of anatomy, embryology, and evolution. To further investigate the molecular organization of the adult mammalian brain, we have built a gene expression-based brain map. We measured gene expression patterns for 24 neural ti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zapala MA,Hovatta I,Ellison JA,Wodicka L,Del Rio JA,Tennant R,Tynan W,Broide RS,Helton R,Stoveken BS,Winrow C,Lockhart DJ,Reilly JF,Young WG,Bloom FE,Lockhart DJ,Barlow C

更新日期：2005-07-19 00:00:00

abstract：:Ferulic, caffeic, chlorogenic, and ellagic acids, four naturally occurring plant phenols, inhibit the mutagenicity and cytotoxicity of (+/-)-7beta,8alpha-dihydroxy-9alpha, 10alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (B[a]P 7,8-diol-9,10-epoxide-2), the only known ultimate carcinogenic metabolite of benzo[a]pyrene....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wood AW,Huang MT,Chang RL,Newmark HL,Lehr RE,Yagi H,Sayer JM,Jerina DM,Conney AH

更新日期：1982-09-01 00:00:00

abstract：:Mutations in fibroblast growth factor receptor 2 (FGFR2) and its ligand, FGF10, are known to cause lacrimo-auriculo-dento-digital (LADD) syndrome. Multiple gain-of-function mutations in FGF receptors have been implicated in a variety of severe skeletal disorders and in many cancers. We aimed to elucidate the mechanism...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lew ED,Bae JH,Rohmann E,Wollnik B,Schlessinger J

更新日期：2007-12-11 00:00:00

abstract：:When we reach toward objects, we easily avoid potential obstacles located in the workspace. Previous studies suggest that obstacle avoidance relies on mechanisms in the dorsal visual stream in the posterior parietal cortex. One fundamental question that remains unanswered is where the visual inputs to these dorsal-str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Striemer CL,Chapman CS,Goodale MA

更新日期：2009-09-15 00:00:00

abstract：:Injection of antisense oligonucleotides was used to investigate the function of c-mos in murine oocytes. Oocytes injected with antisense c-mos oligonucleotides completed the first meiotic division but failed to initiate meiosis II. Instead, loss of c-mos function led to chromosome decondensation, reformation of a nucl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Keefe SJ,Wolfes H,Kiessling AA,Cooper GM

更新日期：1989-09-01 00:00:00

abstract：:The conversion of light energy into ion gradients across biological membranes is one of the most fundamental reactions in primary biological energy transduction. Recently, the structure of the first light-activated Na+ pump, Krokinobacter eikastus rhodopsin 2 (KR2), was resolved at atomic resolution [Kato HE, et al. (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suomivuori CM,Gamiz-Hernandez AP,Sundholm D,Kaila VRI

更新日期：2017-07-03 00:00:00

abstract：:In target tissue extracts, heat shock protein hsp90 has been found associated to all unliganded steroid receptors. Modulation of important functions of these receptors, including prevention of DNA binding and optimization of transcriptional activity, has been attributed to hsp90. However no unequivocal in vivo demonst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang KI,Devin J,Cadepond F,Jibard N,Guiochon-Mantel A,Baulieu EE,Catelli MG

更新日期：1994-01-04 00:00:00

abstract：:Photosynthesis has recently gained considerable attention for its potential role in the development of renewable energy sources. Optimizing photosynthetic organisms for biomass or biofuel production will therefore require a systems understanding of photosynthetic processes. We reconstructed a high-quality genome-scale...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nogales J,Gudmundsson S,Knight EM,Palsson BO,Thiele I

更新日期：2012-02-14 00:00:00

abstract：:In contrast with speciation in terrestrial organisms, marine plankton frequently display gradual morphological change without lineage division (e.g., phyletic gradualism or gradual evolution), which has raised the possibility that a different mode of evolution dominates within pelagic environments. Here, we reexamine ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hull PM,Norris RD

更新日期：2009-12-15 00:00:00

abstract：:To find the native conformation (fold), proteins sample a subspace that is typically hundreds of orders of magnitude smaller than their full conformational space. Whether such fast folding is intrinsic or the result of natural selection, and what is the longest foldable protein, are open questions. Here, we derive the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin MM,Zewail AH

更新日期：2012-06-19 00:00:00

abstract：:Higher-level cognitive processes strongly depend on a complex interplay between mediodorsal thalamus nuclei and the prefrontal cortex (PFC). Alteration of thalamofrontal connectivity has been involved in cognitive deficits of schizophrenia. Prefrontal serotonin (5-HT)2A receptors play an essential role in cortical net...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barre A,Berthoux C,De Bundel D,Valjent E,Bockaert J,Marin P,Bécamel C

更新日期：2016-03-08 00:00:00

abstract：:Messenger RNA from rat liver was partially purified by chromatography on cellulose on the basis of its poly(A) content. Microgram amounts of this RNA stimulate protein synthesis manyfold in a heterologous cell-free system, derived from Krebs ascites cells supplemented with reticulocyte initiation factors. The messenge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schutz G,Beato M,Feigelson P

更新日期：1973-04-01 00:00:00

abstract：:An exciton is an electron-hole pair bound by attractive Coulomb interaction. Short-lived excitons have been detected by a variety of experimental probes in numerous contexts. An excitonic insulator, a collective state of such excitons, has been more elusive. Here, thanks to Nernst measurements in pulsed magnetic field...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang J,Nie P,Li X,Zuo H,Fauqué B,Zhu Z,Behnia K

更新日期：2020-12-01 00:00:00

abstract：:RNA helicases play roles in various essential biological processes such as RNA splicing and editing. Recent in vitro studies show that RNA helicases are involved in immune responses toward viruses, serving as viral RNA sensors or immune signaling adaptors. However, there is still a lack of in vivo data to support the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,He K,Sheng B,Lei X,Tao W,Zhu X,Wei Z,Fu R,Wang A,Bai S,Zhang Z,Hong N,Ye C,Tian Y,Wang J,Li M,Zhang K,Li L,Yang H,Li HB,Flavell RA,Zhu S

更新日期：2021-01-26 00:00:00

abstract：:Mammalian class A macrophage-specific scavenger receptors (SR-A) exhibit unusually broad binding specificity for a wide variety of polyanionic ligands. The properties of these receptors suggest that they may be involved in atherosclerosis and host defense. We have previously observed a similar receptor activity in Dro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pearson A,Lux A,Krieger M

更新日期：1995-04-25 00:00:00

abstract：:Apurinic/apyrimidinic (AP) sites are common DNA lesions that arise from spontaneous depurination or by base excision repair (BER) of modified bases. A biotin-containing aldehyde-reactive probe (ARP) [Kubo, K., Ide, H., Wallace, S. S. & Kow, Y. W. (1992) Biochemistry 31, 3703-3708] is used to measure AP sites in living...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Atamna H,Cheung I,Ames BN

更新日期：2000-01-18 00:00:00

abstract：:Pain is a major health concern even though numerous analgesic agents are available. Side effects and lack of wide-spectrum efficacy of current drugs justify efforts to better understand pain mechanisms. Stabilization of natural epoxy-fatty acids (EFAs) through inhibition of the soluble epoxide hydrolase (sEH) reduces ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inceoglu B,Wagner K,Schebb NH,Morisseau C,Jinks SL,Ulu A,Hegedus C,Rose T,Brosnan R,Hammock BD

更新日期：2011-03-22 00:00:00

abstract：:Guanylyl cyclase C (GCC) has been detected only in intestinal mucosa and colon carcinoma cells of placental mammals. However, this receptor has been identified in several tissues in marsupials, and its expression has been suggested in tissues other than intestine in placental mammals. Selective expression of GCC by co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrithers SL,Barber MT,Biswas S,Parkinson SJ,Park PK,Goldstein SD,Waldman SA

更新日期：1996-12-10 00:00:00

abstract：:K+ channels, which have been linked to regulation of electrogenic solute transport as well as Ca2+ influx, represent a locus in hepatocytes for the concerted actions of hormones that employ Ca2+ and cAMP as intracellular messengers. Despite considerable study, the single-channel basis for synergistic effects of Ca2+ a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lidofsky SD

更新日期：1995-07-18 00:00:00

abstract：:The effects of selected heme analogues on heme oxygenase activity in tissues and on human and rabbit bone marrow hematopoietic colony growth were examined. Zinc protoporphyrin (ZnPP) and zinc mesoporphyrin (ZnMP), at concentrations ranging between 1 and 20 microM, produced significant inhibition of human and rabbit bo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lutton JD,Abraham NG,Drummond GS,Levere RD,Kappas A

更新日期：1997-02-18 00:00:00

abstract：:Across phyla, the ribosomes-the central molecular machines for translation of genetic information-exhibit an overall preserved architecture and a conserved functional core. The natural heterogeneity of the ribosome periodically phases a debate on their functional specialization and the tissue-specific variations of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amirbeigiarab S,Kiani P,Velazquez Sanchez A,Krisp C,Kazantsev A,Fester L,Schlüter H,Ignatova Z

更新日期：2019-11-05 00:00:00

abstract：:Caseinolytic proteases (ClpPs) are large oligomeric protein complexes that contribute to cell homeostasis as well as virulence regulation in bacteria. Although most organisms possess a single ClpP protein, some organisms encode two or more ClpP isoforms. Here, we elucidated the crystal structures of ClpP1 and ClpP2 fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeiler E,List A,Alte F,Gersch M,Wachtel R,Poreba M,Drag M,Groll M,Sieber SA

更新日期：2013-07-09 00:00:00

abstract：:We explore the interactions of CYT-19, a DExD/H-box protein that functions in folding of group I RNAs, with a well characterized misfolded species of the Tetrahymena ribozyme. Consistent with its function, CYT-19 accelerates refolding of the misfolded RNA to its native state. Unexpectedly, CYT-19 performs another reac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tijerina P,Bhaskaran H,Russell R

更新日期：2006-11-07 00:00:00