VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy.

abstract：:Widespread belief in misinformation circulating online is a critical challenge for modern societies. While research to date has focused on psychological and political antecedents to this phenomenon, few studies have explored the role of digital media literacy shortfalls. Using data from preregistered survey experiment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guess AM,Lerner M,Lyons B,Montgomery JM,Nyhan B,Reifler J,Sircar N

更新日期：2020-07-07 00:00:00

abstract：:Phosphatase PP2A expression levels are positively correlated to the clinical severity of systemic lupus erythematosus (SLE) and IL17A cytokine overproduction, indicating a potential role of PP2A in controlling TH17 differentiation and inflammation. By generating a mouse strain with ablation of the catalytic subunit α ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu Q,Jin X,Zheng M,Rohila D,Fu G,Wen Z,Lou J,Wu S,Sloan R,Wang L,Hu H,Gao X,Lu L

更新日期：2019-01-15 00:00:00

abstract：:Food is a powerful entrainment cue for circadian clocks in peripheral tissues, and changes in the composition of nutrients have been demonstrated to metabolically reprogram peripheral clocks. However, how food challenges may influence circadian metabolism of the master clock in the suprachiasmatic nucleus (SCN) or in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tognini P,Samad M,Kinouchi K,Liu Y,Helbling JC,Moisan MP,Eckel-Mahan KL,Baldi P,Sassone-Corsi P

更新日期：2020-11-24 00:00:00

abstract：:Glutamine synthetase (GS) is expressed in the neural retina only in Muller glia cells and is inducible with cortisol. A chicken genomic clone that contains at least part of the coding region for the GS enzyme was used to investigate developmental changes in the level of GS mRNA in embryonic chicken retina. A major GS ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vardimon L,Fox LE,Moscona AA

更新日期：1986-12-01 00:00:00

abstract：:Freshwater planarian flatworms are capable of regenerating complete organisms from tiny fragments of their bodies; the basis for this regenerative prowess is an experimentally accessible stem cell population that is present in the adult planarian. The study of these organisms, classic experimental models for investiga...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Newmark PA,Reddien PW,Cebrià F,Sánchez Alvarado A

更新日期：2003-09-30 00:00:00

abstract：:HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim Y,Ye Z,Joachimiak G,Videau P,Young J,Hurd K,Callahan SM,Gornicki P,Zhao J,Haselkorn R,Joachimiak A

更新日期：2013-05-07 00:00:00

abstract：:We have studied the properties of r-eag voltage-activated potassium channels in a stably transfected human embryonic kidney cell line. It was found that r-eag channels are rapidly and reversibly inhibited by a rise in intracellular calcium from 30 to 300 nM. The inhibition does not appear to depend on the activity of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stansfeld CE,Röper J,Ludwig J,Weseloh RM,Marsh SJ,Brown DA,Pongs O

更新日期：1996-09-03 00:00:00

abstract：:Delta 5-3-Ketosteroid isomerase (EC 5.3.3.1) promotes an allylic rearrangement involving intramolecular proton transfer via a dienolic intermediate. This enzyme enhances the catalytic rate by a factor of 10(10). Two residues, Tyr-14, the general acid that polarizes the steroid 3-carbonyl group and facilitates enolizat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Q,Abeygunawardana C,Talalay P,Mildvan AS

更新日期：1996-08-06 00:00:00

abstract：:Angiogenesis restores blood flow to healing tissues, a process that is inhibited by high doses of glucocorticoids. However, the role of endogenous glucocorticoids and the potential for antiglucocorticoid therapy to enhance angiogenesis is unknown. Using in vitro and in vivo models of angiogenesis in mice, we examined ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Small GR,Hadoke PW,Sharif I,Dover AR,Armour D,Kenyon CJ,Gray GA,Walker BR

更新日期：2005-08-23 00:00:00

abstract：:Seed dormancy is a trait of considerable adaptive significance because it maximizes seedling survival by preventing premature germination under unfavorable conditions. Understanding how seeds break dormancy and initiate growth is also of great agricultural and biotechnological interest. Abscisic acid (ABA) plays prima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lopez-Molina L,Mongrand S,Chua NH

更新日期：2001-04-10 00:00:00

abstract：:The secreted nodulation-signaling protein NodO was purified from the supernatant of cultures of Rhizobium leguminosarum biovar viciae. The native protein has a M(r) of approximately 67,000, suggesting that it exists as a dimer since the DNA sequence predicts a M(r) of 30,002. Pure NodO protein had no protease, pectina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sutton JM,Lea EJ,Downie JA

更新日期：1994-10-11 00:00:00

abstract：:Human 15-lipoxygenase (h15-LO) is present on chromosome 17p13.3 in close proximity to the tumor-suppressor gene, p53. 15-LO is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. The murine BALB/c embryo fibroblast cell line, (10)1val, expresses p53 in mutant (mt) conformation when ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelavkar UP,Badr KF

更新日期：1999-04-13 00:00:00

abstract：:It is shown that polyphosphates are not generated in significant amounts in the phosphoglycerate kinase reaction; polyphosphate is more effective than ATP in the formation of glucose 6-P by glucokinase, but the rate with ATP may be adequate to meet the requirements of glucose metabolism; PPi is far more effective than...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wood HG,Goss NH

更新日期：1985-01-01 00:00:00

abstract：:EF-hand Ca(II) binding sites share a conserved architecture and are prevalent in Ca(II) signaling pathways. The ion binding kinetics of these sites are carefully tuned to provide the physiologically appropriate activation and inactivation time scales. Here we examine kinetic tuning by the side chain at the ninth posit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renner M,Danielson MA,Falke JJ

更新日期：1993-07-15 00:00:00

abstract：:Our ability to interpret seismic observations including the seismic discontinuities and the density and velocity profiles in the earth's interior is critically dependent on the accuracy of pressure measurements up to 364 GPa at high temperature. Pressure scales based on the reduced shock-wave equations of state alone ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fei Y,Ricolleau A,Frank M,Mibe K,Shen G,Prakapenka V

更新日期：2007-05-29 00:00:00

abstract：:The mitochondrial enzyme glutaminase C (GAC) catalyzes the hydrolysis of glutamine to glutamate plus ammonia, a key step in the metabolism of glutamine by cancer cells. Recently, we discovered a class of allosteric inhibitors of GAC that inhibit cancer cell growth without affecting their normal cellular counterparts, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stalnecker CA,Ulrich SM,Li Y,Ramachandran S,McBrayer MK,DeBerardinis RJ,Cerione RA,Erickson JW

更新日期：2015-01-13 00:00:00

abstract：:The receptor-like tyrosine kinase (Ryk), a Wnt receptor, is important for cell fate determination during corticogenesis. During neuronal differentiation, the Ryk intracellular domain (ICD) is cleaved. Cleavage of Ryk and nuclear translocation of Ryk-ICD are required for neuronal differentiation. However, the mechanism...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang WH,Choi SH,Moon BS,Cai M,Lyu J,Bai J,Gao F,Hajjali I,Zhao Z,Campbell DB,Weiner LP,Lu W

更新日期：2017-12-12 00:00:00

abstract：:Across North America, human activities have been shown to cause river incision into unconsolidated alluvium. Human-caused erosion through bedrock, however, has only been observed in local and isolated outcrops. Here, we test whether splash-dam logging, which decreased in-stream alluvial cover by removing much of the a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schanz SA,Montgomery DR,Collins BD

更新日期：2019-04-30 00:00:00

abstract：:Ocular cicatricial pemphigoid (OCP) is an autoimmune disease that affects mainly conjunctiva and other squamous epithelia. OCP is histologically characterized by a separation of the epithelium from underlying tissues within the basement membrane zone. Immunopathological studies demonstrate the deposition of anti-basem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tyagi S,Bhol K,Natarajan K,Livir-Rallatos C,Foster CS,Ahmed AR

更新日期：1996-12-10 00:00:00

abstract：:The amphiphilic fluorescent styryl pyridinium dyes FM1-43 and FM4-64 are used to probe activity-dependent synaptic vesicle cycling in neurons. Cultured astrocytes can internalize FM1-43 and FM4-64 inside vesicles but their uptake is insensitive to the elevation of cytosolic calcium (Ca(2+)) concentration and the under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li D,Hérault K,Oheim M,Ropert N

更新日期：2009-12-22 00:00:00

abstract：:Voltage-gated channels maintain cellular resting potentials and generate neuronal action potentials by regulating ion flux. Here, we show that Ether-à-go-go (EAG) K+ channels also regulate intracellular signaling pathways by a mechanism that is independent of ion flux and depends on the position of the voltage sensor....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hegle AP,Marble DD,Wilson GF

更新日期：2006-02-21 00:00:00

abstract：:Arsenic poisoning affects millions of people worldwide. Human arsenic intake from rice consumption can be substantial because rice is particularly efficient in assimilating arsenic from paddy soils, although the mechanism has not been elucidated. Here we report that two different types of transporters mediate transpor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma JF,Yamaji N,Mitani N,Xu XY,Su YH,McGrath SP,Zhao FJ

更新日期：2008-07-22 00:00:00

abstract：:Fungi produce numerous low molecular weight molecules endowed with a multitude of biological activities. However, mining the full-genome sequences of fungi indicates that their potential to produce secondary metabolites is greatly underestimated. Because most of the biosynthesis gene clusters are silent under laborato...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schroeckh V,Scherlach K,Nützmann HW,Shelest E,Schmidt-Heck W,Schuemann J,Martin K,Hertweck C,Brakhage AA

更新日期：2009-08-25 00:00:00

abstract：:A full-length cDNA clone encoding a glutamate receptor was isolated from a human brain cDNA library, and the gene product was characterized after expression in Xenopus oocytes. Degenerate PCR primers to conserved regions of published rat brain glutamate receptor sequences amplified a 1-kilobase fragment from a human b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun W,Ferrer-Montiel AV,Schinder AF,McPherson JP,Evans GA,Montal M

更新日期：1992-02-15 00:00:00

abstract：:The RIG-G gene, originally isolated from an acute promyelocytic leukemia cell line NB4, codes for a 60-kDa cytoplasmic protein that is induced by all-trans retinoic acid (ATRA) treatment along with the induction of morphological differentiation of NB4 cells. Here, we provide evidence that ectopic expression of Rig-G i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao S,Li D,Zhu HQ,Song MG,Pan XR,Jia PM,Peng LL,Dou AX,Chen GQ,Chen SJ,Chen Z,Tong JH

更新日期：2006-10-31 00:00:00

abstract：:The C-C chemokine receptor 5 (CCR5) plays a crucial role in facilitating the entry of macrophage-tropic strains of the HIV-1 into cells, but the mechanism of this phenomenon is completely unknown. To explore the role of CCR5-derived signal transduction in viral entry, we introduced mutations into two cytoplasmic domai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gosling J,Monteclaro FS,Atchison RE,Arai H,Tsou CL,Goldsmith MA,Charo IF

更新日期：1997-05-13 00:00:00

abstract：:Changes in tree growth rates can affect tree mortality and forest feedbacks to the global carbon cycle. As air temperature increases, evaporative demand also increases, increasing effective drought in forest ecosystems. Using a spatially comprehensive network of Douglas fir (Pseudotsuga menziesii) chronologies from 12...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Restaino CM,Peterson DL,Littell J

更新日期：2016-08-23 00:00:00

abstract：:Although it is now more than two decades since it was first reported that the imidazobenzodiazepine Ro15-4513 reverses behavioral alcohol effects, the molecular target(s) of Ro15-4513 and the mechanism of alcohol antagonism remain elusive. Here, we show that Ro15-4513 blocks the alcohol enhancement on recombinant "ext...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallner M,Hanchar HJ,Olsen RW

更新日期：2006-05-30 00:00:00

abstract：:Noncompetitive inhibitors of the nicotinic acetylcholine (ACh) receptors suppress cation flux directly by binding in and blocking the open channel or indirectly by stabilizing closed states of the receptor. The lidocaine derivative QX-314 and the acridine derivative quinacrine act directly as open channel blockers, bu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu Y,Shi L,Karlin A

更新日期：2003-04-01 00:00:00

abstract：:Oxidative stress plays a central role in many human diseases and in aging. In Caenorhabditis elegans the SKN-1 protein induces phase II detoxification gene transcription, a conserved oxidative stress response, and is required for oxidative stress resistance and longevity. Oxidative stress induces SKN-1 to accumulate i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： An JH,Vranas K,Lucke M,Inoue H,Hisamoto N,Matsumoto K,Blackwell TK

更新日期：2005-11-08 00:00:00