Abstract:
:We have studied the properties of r-eag voltage-activated potassium channels in a stably transfected human embryonic kidney cell line. It was found that r-eag channels are rapidly and reversibly inhibited by a rise in intracellular calcium from 30 to 300 nM. The inhibition does not appear to depend on the activity of calcium-dependent kinases and phosphatases. The effect of calcium on r-eag channel activity was studied in inside-out membrane patches. Calcium inhibited r-eag channel activity with a mean IC50 of 67 nM. Activation of muscarinic receptors, generating calcium oscillations in the transfected cells, induced a synchronous inhibition of r-eag mediated outward currents. This shows that calcium can mediate r-eag current inhibition following muscarinic receptor activation. The data indicate that r-eag channels are calcium-inhibitable voltage-activated potassium channels.
journal_name
Proc Natl Acad Sci U S Aauthors
Stansfeld CE,Röper J,Ludwig J,Weseloh RM,Marsh SJ,Brown DA,Pongs Odoi
10.1073/pnas.93.18.9910subject
Has Abstractpub_date
1996-09-03 00:00:00pages
9910-4issue
18eissn
0027-8424issn
1091-6490journal_volume
93pub_type
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