Abstract：
:Cellular senescence is regulated by specific genes in many organisms. The identification and functional analysis of senescence-associated genes could provide valuable insights into the senescence process. Here, we employed a new and improved differential display reverse transcription-polymerase chain reaction (DDRT-PCR) method that involves annealing control primers (ACPs) to identify genes that are differentially expressed in human umbilical endothelial cells during replicative senescence. Using 120 ACPs, we identified 31 differentially expressed genes (DEGs). Basic local alignment search tool (BLAST) search revealed 29 known genes and two unknown genes. Expression levels of the 29 known genes were confirmed by real-time quantitative RT-RCR and by Western blotting for eight of these genes. CD9 antigen, MHC class I chain-related sequence A (MICA) and cell division cycle 37 homolog (CDC37) were up-regulated, and bone morphogenetic protein 4 (BMP4), dickkopf-1 (DKK1), and transcription factor 7-like 1 (TCF7L1) were down-regulated in old cells. Treatment with recombinant human MICA caused a decrease in cell proliferation and an increase in senescence-associated beta-galactosidase staining. Further analysis of differentially expressed genes may provide insights into the molecular basis of replicative senescence and vascular diseases associated with cellular senescence.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Kim TW,Kim HJ,Lee C,Kim HY,Baek SH,Kim JH,Kwon KS,Kim JRdoi
10.1016/j.exger.2007.12.010subject
Has Abstractpub_date
2008-04-01 00:00:00pages
286-95issue
4eissn
0531-5565issn
1873-6815pii
S0531-5565(08)00003-Xjournal_volume
43pub_type
杂志文章abstract：:A micromethod was used to study myelin from individual rat optic nerves as a function of age. Protein and galactolipid content as well as 2',3'-cyclic nucleotide phosphohydrolase (CNPase) activity at ages 2, 4, 22, and 28 months were determined. Protein content increased significantly at 22 months and decreased at adv...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(89)90013-2
更新日期：1989-01-01 00:00:00
abstract：:We previously demonstrated that in vitro stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes, class switch recombination (CSR), induction of the E2A-encoded transcription factor E47, and activation-induced cytidine deaminase (AID) which is necessary for CSR and ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2006.09.003
更新日期：2007-03-01 00:00:00
abstract：OBJECTIVE:Mitochondria produce cellular energy via oxidative phosphorylation (OXPHOS), mediated by respiratory chain complexes I to IV and ATP synthase (complex V). Mitochondrial respiratory complexes have been shown to decline with age in several tissues. As the intestinal epithelium is a tissue with a high energy dem...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2020.110924
更新日期：2020-07-01 00:00:00
abstract：:We suggest that the processes that protect the turtle brain against anoxia and subsequent reoxygenation might also contribute to turtle longevity since many of them are linked to age related neurodegeneration. In the turtle the mechanisms for conserving ion channel function are particularly robust. The anoxic turtle b...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(03)00111-6
更新日期：2003-07-01 00:00:00
abstract：:The objective of this study was to determine whether and to what extent age-related changes occur in atrioventricular (AV) heart valve innervation. The AV valves from three young adult (3 months) and three older (> 24 months) female guinea pigs were studied. An acetylcholinesterase (AChE) localization method was used ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(94)90037-x
更新日期：1994-09-01 00:00:00
abstract：:A series of theoretical analyses of mortality were performed on recent data for Mediterranean fruit flies (Ceratitus capitata). Best fit computations were performed using the Marquardt-Levenburg technique on: 1) the classic Gompertz model, 2) the Johnson-Mehl-Avrami (JMA) model from chemical kinetics theory, which is ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(94)90051-5
更新日期：1994-03-01 00:00:00
abstract：:Previous studies have shown that adult mice increase cold-induced heat production as a result of repeated exposures to cold, but that aged mice do not. The objective of the present study was to investigate changes in heat loss during repeated cold exposures in adult and aged C57BL/6J mice. Mice were partially restrain...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(96)00131-3
更新日期：1997-05-01 00:00:00
abstract：:Numerous trials have shown that physical activity and exercise training have beneficial effects in general older populations. However, few have studied its effectiveness among people with dementia. The aim of this systematic review is to examine the efficacy of trials using a rigorous randomised, controlled design and...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/j.exger.2012.08.008
更新日期：2013-01-01 00:00:00
abstract：INTRODUCTION:Right ventricular dysfunction (RVD), submassive pulmonary embolism (PE), elevated systolic pulmonary artery pressure (sPAP), elevated cardiac troponin I (cTnI) and old age are well-known risk factors for poor outcome in acute normotensive PE. The aim of this analysis was to calculate age cut-off values to ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2015.05.007
更新日期：2015-09-01 00:00:00
abstract：:Understanding telomere biology is of utmost importance for aging and cancer research. An essential tool is the determination of telomere length, which traditionally is done by telomere restriction fragment analysis, a laborious and time consuming method. Therefore, large efforts have been made to establish alternative...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2005.09.013
更新日期：2006-02-01 00:00:00
abstract：:A non-enzymatic reaction between ketones or aldehydes and the amino groups of proteins, lipids and nucleic acids contributes to the aging of macromolecules and to the development and progression of various age-related disorders such as vascular complications of diabetes, Alzheimer's disease, cancer growth and metastas...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/j.exger.2010.11.007
更新日期：2011-04-01 00:00:00
abstract：:Aromatase inhibitors have become the standard of care for the adjuvant treatment of postmenopausal, hormone-sensitive breast cancer. Meanwhile, more and more breast cancer patients who are treated with aromatase inhibitors as adjuvant therapies often experience arthralgias and musculoskeletal aching, in some cases, ha...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2009.03.003
更新日期：2009-06-01 00:00:00
abstract：:The aging process is known to be regulated by specific genes in various organisms, including yeast, the nematode C. elegans, fruitflies and mice. To explore the novel genes involved in aging process, we applied cDNA microarray technology to a replicative senescence model of human dermal fibroblasts (HDF). Eighty-four ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2004.07.002
更新日期：2004-09-01 00:00:00
abstract：:We develop a theoretical model from an energetic viewpoint for unraveling the entangled effects of metabolic and biosynthetic rates on oxidative cellular damage accumulation during animal's growth, and test the model by experiments in hornworms. The theoretical consideration suggests that most of the cellular damages ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2016.05.015
更新日期：2016-09-01 00:00:00
abstract：:Aging is the major risk factor for numerous brain diseases. This is especially true for Alzheimer's disease (AD), a peculiar neurodegenerative disorder in that it results from the synergy of two simultaneous but distinct degenerating processes: A beta and tau pathologies. For AD, and for most neurodegenerative disorde...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/j.exger.2003.09.010
更新日期：2003-11-01 00:00:00
abstract：:Aging in a given individual can be characterized by the number of deficits (symptoms, signs, laboratory abnormalities, disabilities) that they accumulate. The number of accumulated deficits, more than their nature, well characterizes health status in individuals - the proportion of deficits present in an individual to...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2012.06.015
更新日期：2012-12-01 00:00:00
abstract：:Impaired tolerance to ischemia-reperfusion in older hearts may stem in part from alterations in purine catabolism, impacting on maintenance of energy state and protective signaling via extracellular adenosine. We characterized effects of aging on normoxic and post-ischemic purine metabolism in hearts from young (2-4 m...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2003.08.003
更新日期：2003-10-01 00:00:00
abstract：:Like the weather, many people talk about aging, but no one seems able to do anything about it. This implacability derives, at least in part, from our inability to provide a conceptual base from which analytic insight might derive. The emerging field of nonlinear thermodynamics presents a new view of the universe. It r...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(86)90039-2
更新日期：1986-01-01 00:00:00
abstract：INTRODUCTION:Age-related decreases in cerebral blood flow (CBF) may lead to cognitive decline, while physical activity (PA) can maintain CBF and cognition in aging. The intensity of PA needed to affect CBF in aging, and the independent effects of sedentary time on CBF are currently unknown. Moreover, research conducted...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2019.110679
更新日期：2019-10-01 00:00:00
abstract：:Cellular aging plays a role in longevity and senescence, and has been implicated in medical and psychiatric conditions, including heart disease, cancer, major depression and posttraumatic stress disorder. Telomere shortening and mitochondrial dysfunction are thought to be central to the cellular aging process. The pre...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2015.04.002
更新日期：2015-06-01 00:00:00
abstract：:Aging has a profound effect on the immune system, and both innate and adaptive arms of the immune system show functional decline with age. In response to infection with intracellular microorganisms, old animals mobilize decreased numbers of antigen-specific CD8+ T cells with reduced production of effector molecules an...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/j.exger.2018.01.011
更新日期：2018-05-01 00:00:00
abstract：:Collagens and elastin are 'building blocks' of tissues and extracellular matrix. Mutations in these proteins cause severe congenital syndromes. Adverse genetic variations may accelerate the aging process in adults contributing to premature morbidity, disability and/or mortality. Favorable variants may contribute to lo...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2019.110781
更新日期：2020-01-01 00:00:00
abstract：:It is well established that during early life the thymus gland and the neuroendocrine system influence each other's maturation. Furthermore, there is a growing body of evidence indicating that the immune and neuroendocrine systems also function as a bidirectional network during adult life. In order to assess possible ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(90)90046-5
更新日期：1990-01-01 00:00:00
abstract：:Ageing is a complex process that in muscle in usually associated with a decrease in mass, strength, and velocity of contraction. One of the most striking effects of ageing on muscle is known as sarcopenia, a process that is the result of many cellular changes, such as a reduction in the number of motor units coupled w...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2003.09.012
更新日期：2004-01-01 00:00:00
abstract：:Prior to three months of age there is little melatonin (MLT) secretion in humans. MLT production then commences, becomes circadian, and reaches its highest nocturnal blood levels between the ages of one to three years. During the remainder of childhood, nocturnal peak levels drop progressively by 80%. In adults, these...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/s0531-5565(98)00054-0
更新日期：1998-11-01 00:00:00
abstract：:The aim of the present study was to compare the effects of water-based aerobic training and water-based resistance training on muscular strength and cardiorespiratory fitness in older women. Sixty-nine individuals were randomized into the following three groups: the water-based aerobic training (WA, n = 23) group, the...
journal_title：Experimental gerontology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.exger.2018.04.022
更新日期：2018-07-15 00:00:00
abstract：:Despite well-documented evidence for lifespan extension by methionine restriction (MR), underlying mechanisms remain unknown. As methionine can alter S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH), the substrate and product of DNA methyltransferase-1 (DNMT1), we hypothesized that MR diet alters DNA methyl...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2016.12.003
更新日期：2017-02-01 00:00:00
abstract：:Mitochondrial DNA 5178 adenine/cytosine (mt5178 A/C) polymorphism is one of the longevity-associated mitochondrial DNA polymorphisms. The frequency of the mt5178A genotype is significantly higher in Japanese centenarians than in the general population. We previously reported that serum high-density lipoprotein cholest...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(03)00211-0
更新日期：2003-10-01 00:00:00
abstract：:Age-related behavioral declines are common manifestations of aging in animals. Negative geotaxis, an innate escape response during which flies ascend the wall of a cylinder after being tapped to its bottom, is one of the behaviors that senesces in Drosophila. Many laboratories, including ours, have used a variety of n...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2005.02.005
更新日期：2005-05-01 00:00:00
abstract：:Aging is associated with a change in membrane composition that includes a decrease in membrane polyunsaturated fatty acids, including arachidonic acid, and an increase in membrane cholesterol. Alterations in membrane structure are likely to impact on transmitter release, which relies on the fusion of synaptic plasma a...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(97)00130-7
更新日期：1998-06-01 00:00:00
