Abstract：
:We previously demonstrated that in vitro stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes, class switch recombination (CSR), induction of the E2A-encoded transcription factor E47, and activation-induced cytidine deaminase (AID) which is necessary for CSR and somatic hypermutation. Both anti-CD40 as well as BAFF have been shown to be able to induce CSR. We have investigated the ability of BAFF/IL-4, as compared to anti-CD40/IL-4, to induce CSR to gamma(1) in splenic B cells from young and old mice. We found that anti-CD40/IL-4 is a better CSR stimulus than BAFF/IL-4 in young B cells, as measured by RT-PCR of post-switch transcripts and flow cytometry. CSR is reduced in old B cells and this is independent of the stimulus. AID and gamma(1)PSTs are significantly reduced in old B cells stimulated with anti-CD40/IL-4, but only slightly reduced with BAFF/IL-4. BAFF receptor mRNA expression (BAFF-R, TACI, and BCMA) is not affected by aging. The age-related decrease in CSR induced by anti-CD40/IL-4 is primarily associated with a decrease in E47, whereas the less affected response to BAFF/IL-4 is associated with decreases in both E47 and NF-kappaB. Therefore, NF-kappaB is not involved in the decreased response of old B cells to anti-CD40/IL-4. These differences in B cell responses to CD40/IL-4 and BAFF/IL-4 may help to explain the maintenance of TI vs TD responses in senescent mice.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Frasca D,Riley RL,Blomberg BBdoi
10.1016/j.exger.2006.09.003subject
Has Abstractpub_date
2007-03-01 00:00:00pages
192-203issue
3eissn
0531-5565issn
1873-6815pii
S0531-5565(06)00282-8journal_volume
42pub_type
杂志文章abstract：:As recently reported, it is possible to detect and quantify the amount of the deleted human mitochondrial DNA (mtDNA) in whole blood, platelets and peripheral blood mononuclear cells using real-time PCR. The aim of this study was to identify the cell types in human blood carrying the 4977 bp deleted mtDNA and their ac...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2003.10.011
更新日期：2004-02-01 00:00:00
abstract：:The geriatric populations of many countries are growing rapidly and they present major problems to healthcare infrastructures from both medical and economic perspectives. The elderly are predisposed to a variety of diseases, which contribute to a marked increase in morbidity in this subpopulation. The incidence of liv...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/j.exger.2005.06.009
更新日期：2005-08-01 00:00:00
abstract：:With many caveats to the traditional vertebrate species pertaining to biogerontology investigations, it has been suggested that a most informative model is the one which: 1) examines closely related species, or various members of the same species with naturally occurring lifespan variation, 2) already has adequate med...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/j.exger.2015.08.008
更新日期：2015-11-01 00:00:00
abstract：INTRODUCTION:Aging leads to poorer neuromuscular control that may impact mobility. However, the specific decades when these changes occur, and whether these time-based changes are sex-specific, are unclear. METHODS:Adults aged 20-82 years (N = 93, 51 females) walked six gait trials at their preferred speed over a 10-m...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2018.05.005
更新日期：2018-09-01 00:00:00
abstract：:The aim of this study was to compare the functional and structural changes in similarly identified neurons of the small parietal ganglion in 56 molluscs (Lymnaea stagnalis) of two age groups: adult (10-12 months) and old (20-22 months). No age changes were found in the values of membrane potential, resistance of the n...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(95)00008-5
更新日期：1995-09-01 00:00:00
abstract：:Indigenous DNA adducts (I-compounds) are considered to be a biomarker of aging tissues. Thus far, few studies have been conducted to investigate the accumulation patterns of I-compounds in the brain during aging. Particularly, identities of age-dependent I-compounds have largely remained unknown. In the current study,...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(95)02027-6
更新日期：1996-05-01 00:00:00
abstract：:Gene expression is much altered in aging. We observed age-dependent decline of core clock genes' expression in the whole body of the fruit fly. We hypothesized that inducible overexpression of clock genes (cry, per, tim, cyc and Clk) in the nervous system can improve healthspan of D. melanogaster. We studied the lifes...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2018.11.005
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to compare the effects of 12 weeks of traditional resistance training (TRT) or resistance training using Cluster-set (CS) on functional performance and physical fitness of postmenopausal and elderly women. METHODS:Participants (61.1 ± 4.9 years, body mass 64.5 ± 1.8 kg, height 155.7...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2020.111011
更新日期：2020-09-01 00:00:00
abstract：:DNA binding of the ku protein was investigated in peripheral blood mononuclear cells (PBMC) from 24 subjects of different ages (20-89 years old) displaying age-related changes in DNA repair, mitotic responsiveness, and cytokine production. Ku is an heterodimeric protein composed of two subunits of 70 and 80 kDa, which...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(99)00026-1
更新日期：1999-08-01 00:00:00
abstract：:Delirium is an acute-onset syndrome that exacerbates patients' condition and significantly increases consequential morbidity and mortality. There is no comprehensive, cellular and tissue-level, pathophysiological theory. The melatonin hormone imbalance has been shown to be linked to circadian rhythms, sleep-wake cycle...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2015.09.007
更新日期：2015-12-01 00:00:00
abstract：:Male life history and resource allocation is not frequently studied in aging and life span research. Here, we verify that males of long-lived fruit-feeding butterfly species have reduced longevity on restricted diets [Beck, J., 2007. The importance of amino acids in the adult diet of male tropical rainforest butterfli...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2009.06.008
更新日期：2009-09-01 00:00:00
abstract：:The interrelationship between muscle strength, motor unit (MU) number, and age is poorly understood, and in this study we sought to determine whether age-related differences in muscle strength are moderated by estimates of functioning MU number and size. Eighteen older adults (OA; 67 ± 1.20 years) and 24 young adults ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2013.06.008
更新日期：2013-09-01 00:00:00
abstract：:Alterations in the hypothalamic-pituitary-gonadal axis in females determine the transition from regular to irregular reproductive cycles, with loss of fertility. Stimulation of noradrenergic neurons of the anteroventral periventricular neurons (AVPV) is essential for regular reproductive cycles. Therefore, we examined...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2015.10.003
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND AND PURPOSE:Stroke associated infection (within the first seven days) occurs in approximately half of stroke patients and is associated with a worse prognosis, especially in the elderly. It is uncertain what factors predict stroke associated infection, yet identification of a suitable biomarker for infection...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2013.07.003
更新日期：2013-09-01 00:00:00
abstract：:In aging tissues the oxidative stress increases due to decreased activity of antioxidant enzymes and proteolysis increases due to decreased activity of aminotransferases, which can be modified by hormonal replacement therapy (HRT). The aim of the present study was to determine the effect of HRT on the activities of an...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2005.01.004
更新日期：2005-04-01 00:00:00
abstract：:Alzheimer's disease (AD) is characterized by the accumulation and aggregation of misfolded proteins. The presence of misfolded proteins in the endoplasmic reticulum (ER) triggers a cellular stress response called the unfolded protein response (UPR). Previously, we have shown that the UPR is activated in AD neurons. In...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2006.01.013
更新日期：2006-04-01 00:00:00
abstract：:The purpose of this study was to examine the relative contributions of changes in muscle quality and body composition to changes in lower-extremity physical function (LEPF) following a 6-month exercise and weight loss intervention in overweight and obese older women. Thirty-eight overweight and obese (BMI = 30.0 ± 4.4...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2018.04.011
更新日期：2018-07-15 00:00:00
abstract：:We have identified a multistep mechanism by which the DNA virus SV40 overcomes cellular senescence. Expression of SV40 T antigen is required for both transient extension of life span and unlimited life span or immortalization. These effects are mediated through inactivation of function of growth suppressors pRB and p5...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/0531-5565(95)00024-0
更新日期：1996-01-01 00:00:00
abstract：:To counteract the debilitating progression of sarcopenia, a protein supplement should provide an energetically efficient anabolic stimulus. We quantified net muscle protein synthesis in healthy elderly individuals (65-79 yrs) following ingestion of an isocaloric intact whey protein supplement (WY; n=8) or an essential...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2005.10.006
更新日期：2006-02-01 00:00:00
abstract：:Degeneration of tongue muscles with aging may contribute to swallowing deficits observed in elderly people. However, the capacity for tongue muscle stem cells (SCs) to regenerate and repair the aged tongue and improve tongue strength following tongue exercise (a current clinical treatment) has never been examined. We ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2020.111104
更新日期：2020-12-01 00:00:00
abstract：:We investigated the telomere lengths of individual cell types in lingual mucosa using an improved tissue quantitative fluorescence in situ hybridization (Q-FISH) method. Our tissue Q-FISH method compensates for partially cut nuclei in a tissue section by using the telomere:centromere ratio (TCR). We normalized our TCR...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2008.06.001
更新日期：2008-09-01 00:00:00
abstract：:It is well established that the proliferative potential of the liver declines with aging. Epidermal growth factor (EGF)-stimulated DNA synthesis is reduced in hepatocytes from aged rats relative to young rats, and this reduction correlates with diminished activation of the extracellular signal-regulated kinase (ERK) p...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(02)00239-5
更新日期：2003-04-01 00:00:00
abstract：:Plasma and pituitary concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and prolactin (Prl) were determined by radioimmunoassay in young (2-4 months-old) and aged CD-1 (14-18 months-old) and C57BL/6 (16-22 months-old) mice one month after ovariectomy. In young, ovariectomized mice, plasma ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(82)80005-3
更新日期：1982-01-01 00:00:00
abstract：:A colony of male Lakeview Golden (LVG) Syrian hamsters has been maintained for the last nine years as a source of various tissues for cellular aging studies. Observations on this colony also yielded data on survival time and physical and pathological manifestations of aging in this strain. Based on 150 spontaneous dea...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(90)90032-w
更新日期：1990-01-01 00:00:00
abstract：:Synaptic disturbances may play a key role in the pathophysiology of Alzheimer's disease. To characterize differential synaptic alterations in the brains of Alzheimer patients, chromogranin A, chromogranin B and secretoneurin were applied as soluble constituents for large dense core vesicles, synaptophysin as a vesicle...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2003.09.018
更新日期：2004-01-01 00:00:00
abstract：OBJECTIVES:To evaluate lean mass index (LMI) measured by bioimpedance (BIA) and anthropometry compared to densitometry (DXA) in elderly outpatients from a tertiary care hospital. METHODS:Participants were over 60-year-old men, presenting no dementia or disability, from a tertiary geriatric ambulatory. LMI obtained by ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2020.111020
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND:Self-report questionnaires are widely used to assess physical function in older adults. However, they often lack a clear frame of reference and hence interpreting and rating task difficulty levels can be problematic for the responder. Consequently, the usefulness of traditional self-report questionnaires for...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2016.04.022
更新日期：2016-08-01 00:00:00
abstract：:Melatonin, the main product synthesized by the pineal gland, modulates several brain functions through different mechanisms, some of them involving the activation or participation of calcium binding intracellular proteins, such as the alpha calcium dependent protein kinase C and calmodulin. Another calcium-binding pro...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2014.10.014
更新日期：2014-12-01 00:00:00
abstract：:In order to establish the role of calcitonin (CT) in postmenopausal bone loss, we studied CT metabolism in 25 pre- and postmenopausal women. Postmenopausal women presented a highly significant reduction of CT basal levels compared to premenopausal females (p less than 0.01). Also, production rates of CT in osteoporoti...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(90)90072-a
更新日期：1990-01-01 00:00:00
abstract：:SIRT1 is a highly conserved NAD(+)-dependent protein deacetylase known to have protective effects against a variety of age-related diseases. However, there is a lack of information concerning SIRT1 expression in the cochlea and auditory cortex of C57BL/6 mice, a mouse model of age-related hearing loss. Using RT-PCR an...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2013.12.006
更新日期：2014-03-01 00:00:00
