A chemical genomics screen highlights the essential role of mitochondria in HIF-1 regulation.

abstract：:In experiments originally designed to examine selective turnover of methylated "caps" in renal mRNA, we observed that [3H]methyl label decayed from mRNA containing poly(A) with a half-life of 1-2 hr. (Caps are blocked, methylated mRNA sequences of the general structure m7GpppNm p(1 or 2)Np.). To distinguish between me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ouellette AJ,Reed SL,Malt RA

更新日期：1976-08-01 00:00:00

abstract：:It has been postulated that, in the adult visual cortex, visual inputs modulate levels of mRNAs coding for neurotransmitter receptors in an activity-dependent manner. To investigate this possibility, we performed a monocular enucleation in adult rabbits and, 15 days later, collected their left and right visual cortice...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nguyen QT,Matute C,Miledi R

更新日期：1998-03-17 00:00:00

abstract：:Infective stages of the protozoan parasite Leishmania spp. accumulate a class of beta-1,2-mannan oligosaccharides as their major carbohydrate reserve material. Here, we describe the biosynthesis of Leishmania mannan. Mannan precursors were identified by metabolic labeling of Leishmania mexicana promastigotes with [(3)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sernee MF,Ralton JE,Dinev Z,Khairallah GN,O'Hair RA,Williams SJ,McConville MJ

更新日期：2006-06-20 00:00:00

abstract：:The temporal dynamics of electrical activity in an olfactory organ, the procerebral lobe of the terrestrial mollusc Limax maximus, is studied. The lobe exhibits intrinsic oscillations in its field potential. Intracellular recordings show that the lobe contains two classes of neurons, both with activity phase-locked to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Delaney KR,Gelperin A,Fee MS,Flores JA,Gervais R,Tank DW,Kleinfeld D

更新日期：1994-01-18 00:00:00

abstract：:Dopamine supersensitivity occurs in schizophrenia and other psychoses, and after hippocampal lesions, antipsychotics, ethanol, amphetamine, phencyclidine, gene knockouts of Dbh (dopamine beta-hydroxylase), Drd4 receptors, Gprk6 (G protein-coupled receptor kinase 6), Comt (catechol-O-methyltransferase), or Th-/-, DbhTh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seeman P,Weinshenker D,Quirion R,Srivastava LK,Bhardwaj SK,Grandy DK,Premont RT,Sotnikova TD,Boksa P,El-Ghundi M,O'dowd BF,George SR,Perreault ML,Männistö PT,Robinson S,Palmiter RD,Tallerico T

更新日期：2005-03-01 00:00:00

abstract：:Human genetic studies have given evidence of familial, disease-causing mutations in the analogous amino acid residue shared by three related RNA binding proteins causative of three neurological diseases. Alteration of aspartic acid residue 290 of hnRNPA2 to valine is believed to predispose patients to multisystem prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murray DT,Zhou X,Kato M,Xiang S,Tycko R,McKnight SL

更新日期：2018-10-16 00:00:00

abstract：:The human renin gene was isolated from a Charon 4A human genomic library and characterized. The gene spans about 11.7 kilobases and consists of 10 exons and 9 introns that map at points that could be variable surface loops of the enzyme. The complete coding regions, the 5'- and 3'-flanking regions, and the exon-intron...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyazaki H,Fukamizu A,Hirose S,Hayashi T,Hori H,Ohkubo H,Nakanishi S,Murakami K

更新日期：1984-10-01 00:00:00

abstract：:Cyclic nucleotide metabolism was investigated in growing kidneys of rats during compensatory hypertrophy and during neonatal development. After unilateral nephrectomy a mild and short-lasting decrease in cyclic 3':5" adenosine monophosphate (cAMP) was observed in the hypertrophying kidney. In contrast, cyclic 3':5' gu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schlondorff D,Weber H

更新日期：1976-02-01 00:00:00

abstract：:GTP-tubulin forms a cap on microtubule ends during aggregation. The bulk of the microtubule is GDP-tubulin. This complicates the usual simple kinetic theory of subunit exchange at microtubule ends to such an extent that Monte Carlo calculations are needed to handle the complications, except in special cases. The Monte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Y,Hill TL

更新日期：1983-12-01 00:00:00

abstract：:Two strains that overproduce endonuclease III were found in a colony bank containing hybrid ColE1-Escherichia coli plasmids. The enzyme was identified in crude extracts by the degradation of partially depyrimidinated DNA in the presence of EDTA, by its sedimentation velocity, and by its associated thymine glycol-DNA g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cunningham RP,Weiss B

更新日期：1985-01-01 00:00:00

abstract：:Lateral mobility of an active fluorescent derivative of cytochrome c on the membranes of giant mitochondria was measured by fluorescence redistribution after photobleaching. A diffusion coefficient of 1.6 X 10(-10) cm2/sec was determined for the labeled cytochrome c on inner mitochondrial membranes under conditions wh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hochman JH,Schindler M,Lee JG,Ferguson-Miller S

更新日期：1982-11-01 00:00:00

abstract：:On the basis of earlier energy computations, the various single peptide units in proteins were designated as helix-making or helix-breaking.(1) With the use of these designations, empirical rules for distinguishing between alpha-helical and non-alpha-helical regions of proteins have been formulated. These rules includ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kotelchuck D,Scheraga HA

更新日期：1969-01-01 00:00:00

abstract：:A full-length placental alkaline phosphatase (PLAP) cDNA was used to identify and clone the PLAP-like Nagao isozyme gene from human genomic libraries. The entire nucleotide sequence of the gene reveals the existence of 11 exons interrupted by 10 small introns (76-427 base pairs). Putative regulatory sequences have bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millán JL,Manes T

更新日期：1988-05-01 00:00:00

abstract：:Splenic lymphocytes from a Lewis rat, immunized with purified estradiol-receptor complex of calf uterine nuclei, were fused with cells of three different mouse myeloma lines (P3-X63-Ag8, P3-NSI/I-Ag4-1, and Sp2/0-Ag14) to yield hybridoma cultures, 9% of which produced antibodies to the receptor protein (estrophilin). ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greene GL,Fitch FW,Jensen EV

更新日期：1980-01-01 00:00:00

abstract：:A combined genetic, biochemical, and immunological approach has clarified structural relationships involving the first three enzymes of de novo pyrimidine biosynthesis. A procedure involving antibody and protein A-Sepharose was used to isolate the enzymes carbamoyl-phosphate synthase [ATP:carbamate phosphotransferase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davidson JN,Patterson D

更新日期：1979-04-01 00:00:00

abstract：:Cell couples have been formed by mixing an antigen- and Ia-specific cloned helper T-cell line with a B-cell hybridoma presenting the antigen. By immunofluorescence observations, we have shown that the microtubule-organizing center (MTOC) inside the helper T cell, but not in the bound antigen-presenting cell, becomes o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kupfer A,Swain SL,Janeway CA Jr,Singer SJ

更新日期：1986-08-01 00:00:00

abstract：:Examination of old human lenses indicates that low-molecular-weight alpha crystallin is not present in the inner 30-40%, the nucleus, of the lens. The remainder of such lenses, the periphery, contains normal levels of this protein. This finding is in marked contrast to observations in young lenses, where a large quant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roy D,Spector A

更新日期：1976-10-01 00:00:00

abstract：:Photosynthetic water splitting, coupled to hydrogenase-catalyzed hydrogen production, is considered a promising clean, renewable source of energy. It is widely accepted that the oxygen sensitivity of hydrogen production, combined with competition between hydrogenases and NADPH-dependent carbon dioxide fixation are the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yacoby I,Pochekailov S,Toporik H,Ghirardi ML,King PW,Zhang S

更新日期：2011-06-07 00:00:00

abstract：:Encoding ribonuclease H1 (RNase H1) degrades RNA hybridized to DNA, and its function is essential for mitochondrial DNA maintenance in the developing mouse. Here we define the role of RNase H1 in mitochondrial DNA replication. Analysis of replicating mitochondrial DNA in embryonic fibroblasts lacking RNase H1 reveals ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes JB,Akman G,Wood SR,Sakhuja K,Cerritelli SM,Moss C,Bowmaker MR,Jacobs HT,Crouch RJ,Holt IJ

更新日期：2015-07-28 00:00:00

abstract：:Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemical synthesis of si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu JY,DeRuiter SL,Turner DL

更新日期：2002-04-30 00:00:00

abstract：:Adenosine has been identified in the anterior pituitary gland and is secreted from cultured folliculostellate (FS) cells. To determine whether adenosine controls the secretion of anterior pituitary hormones in vitro, adenosine was incubated with anterior pituitaries. It stimulated prolactin (PRL) release at the lowest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu WH,Kimura M,Walczewska A,Porter JC,McCann SM

更新日期：1998-06-23 00:00:00

abstract：:The Eemian (last interglacial, 130-115 ka) was likely the warmest of all interglacials of the last 800 ka, with summer Arctic temperatures 3-5 °C above present. Here, we present improved Eemian climate records from central Greenland, reconstructed from the base of the Greenland Ice Sheet Project 2 (GISP2) ice core. Ou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yau AM,Bender ML,Robinson A,Brook EJ

更新日期：2016-08-30 00:00:00

abstract：:Oxidative stress plays a central role in many human diseases and in aging. In Caenorhabditis elegans the SKN-1 protein induces phase II detoxification gene transcription, a conserved oxidative stress response, and is required for oxidative stress resistance and longevity. Oxidative stress induces SKN-1 to accumulate i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： An JH,Vranas K,Lucke M,Inoue H,Hisamoto N,Matsumoto K,Blackwell TK

更新日期：2005-11-08 00:00:00

abstract：:Reaction of the normal isomer of [B20H18]2- and the protected thiol anion, [SC(O)OC(CH3)3]-, produces an unexpected isomer of [B20H17SC(O)OC(CH3)3]4- directly and in good yield. The isomer produced under mild conditions is characterized by an apical-apical boron atom intercage connection as well as the location of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feakes DA,Waller RC,Hathaway DK,Morton VS

更新日期：1999-05-25 00:00:00

abstract：:Pentamidine, diminazene aceturate (Berenil), isometamidium chloride (Samorin), and ethidium bromide, which are important antitrypanosomal drugs, promote linearization of Trypanosoma equiperdum minicircle DNA (the principal component of kinetoplast DNA, the mitochondrial DNA in these parasites). This effect occurs at t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shapiro TA,Englund PT

更新日期：1990-02-01 00:00:00

abstract：:The rotational strengths of the four longer wavelength transitions, B(2u), B(1u), and the two E(1u), of adenosine derivatives constrained in the syn form have been investigated theoretically and experimentally. The theory combines a complete neglect of differential overlap version S (CNDO/S) description of the base wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles DW,Farmer M,Eyring H

更新日期：1980-06-01 00:00:00

abstract：:The extensive intratumor heterogeneity revealed by sequencing cancer genomes is an essential determinant of tumor progression, diagnosis, and treatment. What maintains heterogeneity remains an open question because competition within a tumor leads to a strong selection for the fittest subclone. Cancer cells also coope...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Archetti M,Ferraro DA,Christofori G

更新日期：2015-02-10 00:00:00

abstract：:ERK6, a mitogen-activated protein (MAP) kinase-related serine/threonine kinase, is highly expressed in human skeletal muscle and appears to function as a signal transducer during differentiation of myoblasts to myotubes. In transfected 293 cells, activation of the 45-kDa enzyme results in tyrosine-phosphorylated 46- a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lechner C,Zahalka MA,Giot JF,Møller NP,Ullrich A

更新日期：1996-04-30 00:00:00

abstract：:Utilization of transcription factors might be a powerful approach to modification of metabolism for a generation of crops having superior characteristics because a single transcription factor frequently regulates coordinated expression of a set of key genes for respective pathways. Here, we apply the plant-specific Do...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yanagisawa S,Akiyama A,Kisaka H,Uchimiya H,Miwa T

更新日期：2004-05-18 00:00:00

abstract：:Proteins potentiated by epidermal growth factor (EGF) to induce a transformed phenotype in non-neoplastic rat kidney fibroblasts in cell culture have been isolated from many non-neoplastic tissues of the adult mouse, including submaxillary gland, kidney, liver, muscle, heart, and brain. They resemble previously descri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberts AB,Anzano MA,Lamb LC,Smith JM,Sporn MB

更新日期：1981-09-01 00:00:00