Abstract:
:Microvascular endothelial cells from rat lungs were cultured in serum-free medium supplemented with an endothelial growth substance, insulin, hydrocortisone and so on. Five to seven days after plating, cultured cells formed a monolayer. They were identified as endothelial cells by morphology and by positive immunohistochemistry for factor VIII-related antigen, a marker for endothelia cells. Differences between gelatin coated culture plates and plastic culture plates in endothelial cell proliferation were evaluated. Cells plated on uncoated plastic plates had a spindle-shaped morphology and did not express factor VIII-related antigen. Two types of medium, serum-free medium containing endothelial growth substance and basal medium supplemented with 20% fetal calf serum, were also compared in primary culture. In contrast with the serum-free medium, cells cultured in the serum-containing medium showed fibroblast-like morphology and did not express factor VIII-related antigen. These results suggest that a gelatin substratum and serum-free medium containing endothelial growth supplement are necessary for in vitro proliferation of microvascular endothelial cells isolated from rat lungs. The culture method and conditions outlined here allow the proliferation of pure microvascular endothelial cells from rat lungs. It may be useful in studying hematogenous metastasis to the lung and the role of microvascular endothelium in other pulmonary disease.
journal_name
Yonsei Med Jjournal_title
Yonsei medical journalauthors
Kim NS,Kim SJdoi
10.3349/ymj.1991.32.4.303subject
Has Abstractpub_date
1991-12-01 00:00:00pages
303-14issue
4eissn
0513-5796issn
1976-2437journal_volume
32pub_type
杂志文章abstract:PURPOSE:To observe changes in audiology, intellectual development, behavior development, and physical growth during systematic follow-up of infants with asymptomatic congenital human cytomegalovirus (HCMV) infection. MATERIALS AND METHODS:Fifty-two infants diagnosed with asymptomatic congenital HCMV infection from Jul...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2009.50.5.667
更新日期:2009-10-31 00:00:00
abstract:PURPOSE:Imbalances between osteogenic and adipogenic differentiation leads to diseases such as osteoporosis. The aim of our study was to demonstrate the differences in extracellular signal-regulated kinase (ERK) phosphorylation during both adipogenesis and osteogenesis of human bone marrow-derived stem cells (BMSCs). ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2011.52.1.165
更新日期:2011-01-01 00:00:00
abstract::To determine whether the dysfunction of p53 protein, caused either by the mutation of p53 gene itself or by the human papillomavirus (HPVs) is involved in the development of cervical cancers and to find out the status of p53 tumor suppressor gene in HPV positive or negative cancers, we analyzed 64 cases of primary cer...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1995.36.5.412
更新日期:1995-11-01 00:00:00
abstract:PURPOSE:The aim of this study was to determine whether stratification of deceased donors by the United Network for Organ Sharing (UNOS) criteria negatively impacts graft survival. MATERIALS AND METHODS:We retrospectively reviewed deceased donor and recipient pretransplant variables of kidney transplantations that occu...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2017.58.3.626
更新日期:2017-05-01 00:00:00
abstract::Stage IV-S neuroblastoma, characterized by a primary tumor plus disseminated tumors in liver, skin and bone marrow, has a favorable clinical prognosis when compared to metastatic Stage IV neuroblastoma. This favorable outcome also characterized mice receiving tumor transplants to these "IV-S" sites. We report the test...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1992.33.3.258
更新日期:1992-09-01 00:00:00
abstract:PURPOSE:Techniques designed to identify differentially expressed genes (DEGs) in tumors have become important in modern pathology. Genefishing technique using the annealing control primer (ACP) system has recently been developed to screen for DEG transcripts. We tried to identify DEGs involved in papillary thyroid canc...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2009.50.1.60
更新日期:2009-02-28 00:00:00
abstract:PURPOSE:This study examined 2-year outcome of consecutive therapy using entecavir (ETV) followed by telbivudine (LdT) in subjects with undetectable hepatitis B virus (HBV) DNA level and normal alanine aminotransferase level after the initial 6 months of ETV treatment. MATERIALS AND METHODS:Sixty subjects were randomiz...
journal_title:Yonsei medical journal
pub_type: 杂志文章,随机对照试验
doi:10.3349/ymj.2017.58.3.552
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:Elevated C-reactive protein (CRP) is associated with poor prognosis in several tumor types. The purpose of this study was to investigate serum CRP as a prognostic marker in small cell lung cancer (SCLC). MATERIALS AND METHODS:The pretreatment serum CRP level was measured in 157 newly diagnosed SCLC patients, a...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2012.53.1.111
更新日期:2012-01-01 00:00:00
abstract::Lactobacillus sp., generally considered to be a harmless indigenous bacteria of the mucous membrane, occasionally causes serious infections. Lactobacillus endocarditis is a very rare disease, and no case has been reported in Korea. Gram-positive bacilli were isolated from blood cultures of a 41-year-old man with clini...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1991.32.1.69
更新日期:1991-03-01 00:00:00
abstract:PURPOSE:The 2018 PyeongChang Winter Olympic Games involved 2925 elite athletes, and providing proper health care services for these elite athletes was a critical priority. We established an emergency department (ED) in the Pyeongchang Mountain Polyclinic during the Olympics, which served staff and athletes from many co...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2019.60.5.474
更新日期:2019-05-01 00:00:00
abstract::Radiographic sagittal plane analysis of VATS (video-assisted thoracoscopic surgery) anterior instrumentation for adolescent idiopathic scoliosis. This is retrospective study. To report, in details about effects of VATS anterior instrumentation on the sagittal plane. Evaluations of the surgical outcome of scoliosis hav...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2007.48.1.90
更新日期:2007-02-28 00:00:00
abstract::Internal jugular phlebectasia (IJP) is a fusiform dilatation of the internal jugular vein (IJV), usually presented as a neck mass in children. Accurate diagnosis from carefully directed history, physical examination, and radiological study could result in lifesaving therapy. We performed our study to suggest possible ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2002.43.3.329
更新日期:2002-06-01 00:00:00
abstract::Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant muscular disorder characterized by weakness of facial, shoulder, abdominal, hip girdle, humeral, and anterior distal leg muscles, with descending progression from the face to the legs in an asymmetric pattern. In about 5% of patients with FSHD, no ...
journal_title:Yonsei medical journal
pub_type:
doi:10.3349/ymj.2021.62.1.95
更新日期:2021-01-01 00:00:00
abstract:PURPOSE:The present study aimed to evaluate the efficacy and safety of polymer-free drug-coated BioFreedom stent implantation in comparison to coronary artery bypass graft (CABG) before major noncardiac surgery. MATERIALS AND METHODS:In a multicenter registry, 55 patients required revascularization before major noncar...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2018.59.4.480
更新日期:2018-06-01 00:00:00
abstract::Electrophysiologic carpal tunnel syndrome (CTS) is common and is frequently asymptomatic in diabetics. In order to evaluate the clinical significance of asymptomatic electrophysiologic CTS, the nerve conduction studies (NCS) of 48 diabetics with asymptomatic electrophysiologic CTS were compared with those of 56 age an...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2000.41.1.123
更新日期:2000-02-01 00:00:00
abstract:PURPOSE:To investigate the relationship between prostate volume and the increased risk for being diagnosed with prostate cancer (PCa) in men with slowly increasing prostate specific antigen (PSA). MATERIALS AND METHODS:A cohort of 1035 men who visited our hospital's health promotion center and were checked for serum P...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2013.54.5.1202
更新日期:2013-09-01 00:00:00
abstract::Pseudoaneurysm arising from the superficial temporal artery (STA) is very rare and is most commonly caused by blunt trauma. Most pseudoaneurysms of the STA usually present as a painless pulsating mass, with concomitant symptoms according to location, and their size may rapidly increase. The treatment of choice is liga...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1998.39.2.180
更新日期:1998-04-01 00:00:00
abstract::The prevalence of circulating immune complexes (CIC), their role and their relationship to cell-mediated immunity in patients with hepatitis B virus associated liver disease are still controversial. This study was designed to investigate the prevalence of CIC and their relationship to viral markers, to subsets of peri...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1990.31.4.347
更新日期:1990-12-01 00:00:00
abstract:PURPOSE:The objective of this study is to define the clinical implications of consolidations in nodular bronchiectatic type Mycobacterium avium complex (NBMAC) infection. MATERIALS AND METHODS:A total of 69 patients (M : F = 17 : 52; mean age, 64 years; age range, 41-85 years) with MAC isolated in the sputum culture a...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2010.51.4.546
更新日期:2010-07-01 00:00:00
abstract::The purpose of this study was to evaluate the clinical feasibility, benefits, and limitations of CT fluoroscopy (CTF)-guided percutaneous vertebroplasty (PVP). PVP under the guidance of CTF without additional guidance by conventional C-arm fluoroscopy was performed in a total of 29 vertebral bodies in 21 patients with...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2005.46.5.635
更新日期:2005-10-31 00:00:00
abstract::We investigated the changes of CD5+ B cells in the peripheral blood of 20 Kawasaki disease (KD) patients. The percentage of CD5+ B cells in the total lymphocytes and in the total B cells significantly decreased during the acute phase of KD(p < 0.01), compared to that in the age-matched normal control subjects. After i...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1996.37.1.52
更新日期:1996-02-01 00:00:00
abstract::The external elastic lamina (EEL) serves as a barrier for cells and macromolecules between the media and adventitia in the vascular wall. We evaluated the morphological changes and quantitative assessments of the EEL architecture in the coronary circulation of pigs fed with a high cholesterol diet. Confocal microscopy...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1999.40.3.273
更新日期:1999-06-01 00:00:00
abstract::Epidermolysis bullosa acquisita (EBA) is an autoimmune-mediated subepidermal bullous disease in which the target of the autoantibodies is type VII collagen, a major component of anchoring fibrils. The purpose of this study was to evaluate the complement-fixing abilities and IgG subclass distribution of autoantibodies ...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.1998.39.4.339
更新日期:1998-08-01 00:00:00
abstract::Although many weakening procedures for the inferior oblique muscle have been advocated, there is some controversy as to the most beneficial procedure for weakening overacting inferior oblique muscles. This study was undertaken to determine if unilateral anterior transposition of the inferior oblique muscle alone could...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2004.45.4.609
更新日期:2004-08-31 00:00:00
abstract::Streptococcus pneumoniae is an important pathogen causing invasive infections particularly in children. Penicillin-nonsusceptible pneumococci are very prevalent in Korea and a difficult problem in antimicrobial treatment. Immunization with effective vaccines including viral and bacterial vaccines has proven to be the ...
journal_title:Yonsei medical journal
pub_type: 杂志文章,评审
doi:10.3349/ymj.1998.39.6.611
更新日期:1998-12-01 00:00:00
abstract:PURPOSE:A high risk of cardiovascular disease (CVD) is reported for HIV-infected individuals. While a link between abacavir and CVD risk is suggested, an association between abacavir and hypertension remains unclear. This study evaluated hypertension risk with abacavir use in comparison to non-abacavir antiretroviral t...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2018.59.10.1245
更新日期:2018-12-01 00:00:00
abstract::Kinematically aligned total knee arthroplasty (TKA) is a new alignment technique. Kinematic alignment corrects arthritic deformity to the patient's constitutional alignment in order to position the femoral and tibial components, as well as to restore the knee's natural tibial-femoral articular surface, alignment, and ...
journal_title:Yonsei medical journal
pub_type: 杂志文章,评审
doi:10.3349/ymj.2020.61.3.201
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:To explore the effect of bi-parametric MRI-ultrasound (MR/US) fusion prostate biopsy on the detection of overall cancer and significant prostate cancer (sPCa). MATERIALS AND METHODS:We examined 140 patients with suspected prostate cancer lesions on MRI from August 2016 to March 2018. All patients had undergone...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2019.60.4.346
更新日期:2019-04-01 00:00:00
abstract:PURPOSE:Coronary artery spasm (CAS) and diabetes mellitus (DM) are implicated in endothelial dysfunction, and insulin resistance (IR) is a major etiological cause of type 2 DM. However, the association between CAS and IR in non-diabetic individuals has not been elucidated. The aim of the present study was to evaluate t...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2018.59.9.1057
更新日期:2018-11-01 00:00:00
abstract::Sclerosing hemangioma (SH) of the lung is an uncommon type of tumor, which is composed of polygonal and cuboidal cells. This disease is generally regarded as benign but extremely rare cases with lymph node metastasis have been reported. We report a case of SH with a metastasis to the regional lymph nodes. A 19-year-ol...
journal_title:Yonsei medical journal
pub_type: 杂志文章
doi:10.3349/ymj.2003.44.1.150
更新日期:2003-02-01 00:00:00