Abstract：
:Identifying the properties of a molecule involved in the efficient activation of the innate and adaptive immune responses that lead to long-lasting immunity is crucial for vaccine and adjuvant development. Here we show that the papaya mosaic virus (PapMV) is recognized by the immune system as a pathogen-associated molecular pattern (PAMP) and as an antigen in mice (Pamptigen). A single immunization of PapMV without added adjuvant efficiently induced both cellular and specific long-lasting antibody responses. PapMV also efficiently activated innate immune responses, as shown by the induction of lipid raft aggregation, secretion of pro-inflammatory cytokines, up-regulation of co-stimulatory molecules on dendritic cells and macrophages, and long-lasting adjuvant effects upon the specific antibody responses to model antigens. PapMV mixed with Salmonella enterica serovar Typhi (S. typhi) outer membrane protein C increased its protective capacity against challenge with S. typhi, revealing the intrinsic adjuvant properties of PapMV in the induction of immunity. Antigen-presenting cells loaded with PapMV efficiently induced antibody responses in vivo, which may link the innate and adaptive responses observed. PapMV recognition as a Pamptigen might be translated into long-lasting antibody responses and protection observed. These properties could be used in the development of new vaccine platforms.
journal_name
Immunologyjournal_title
Immunologyauthors
Acosta-Ramírez E,Pérez-Flores R,Majeau N,Pastelin-Palacios R,Gil-Cruz C,Ramírez-Saldaña M,Manjarrez-Orduño N,Cervantes-Barragán L,Santos-Argumedo L,Flores-Romo L,Becker I,Isibasi A,Leclerc D,López-Macías Cdoi
10.1111/j.1365-2567.2007.02753.xsubject
Has Abstractpub_date
2008-06-01 00:00:00pages
186-97issue
2eissn
0019-2805issn
1365-2567pii
IMM2753journal_volume
124pub_type
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