Abstract:
:The stimulation of bacteria capable of reducing soluble U(VI) to sparingly soluble U(IV) is a promising approach for containing U(VI) plumes. Anaeromyxobacter dehalogenans is capable of mediating this activity; however, its ability to couple U(VI) reduction to growth has not been established. Monitoring the increase in 16S rRNA gene copy numbers using quantitative real-time PCR (qPCR) in cultures provided with U(VI) as an electron acceptor demonstrated growth, and 7.7-8.6 x 10(6) cells were produced per mumole of U(VI) reduced. This biomass yield was lower than predicted based on the theoretical free energy changes associated with U(VI)-to-U(IV) reduction. Lower than predicted growth yields with U(VI) as electron acceptor were also determined in cultures of Geobacter lovleyi and Geobacter sulfurreducens suggesting that U(VI) reduction is inefficient or imposes an additional cost to growing cells. These findings have implications for U(VI) bioremediation because Anaeromyxobacter spp. and Geobacter spp. contribute to radionuclide immobilization in contaminated subsurface environments.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Sanford RA,Wu Q,Sung Y,Thomas SH,Amos BK,Prince EK,Löffler FEdoi
10.1111/j.1462-2920.2007.01405.xsubject
Has Abstractpub_date
2007-11-01 00:00:00pages
2885-93issue
11eissn
1462-2912issn
1462-2920pii
EMI1405journal_volume
9pub_type
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