Abstract:
:The ADP ribosylation factor (Arf) and the coat protein complex I (COPI) are involved in vesicle transport. Together with GTPase-activating proteins (ArfGAPs) and guanine exchange factors (ArfGEFs) that regulate the activity of Arf, they govern vesicle formation, COPI trafficking and the maintenance of the Golgi complex. In an ongoing effort to study the role of membrane trafficking in pathogenesis of the rice blast fungus Magnaporthe oryzae, we identified MoGlo3 as an ArfGAP protein that is homologous to Glo3p of the budding yeast Saccharomyces cerevisiae. As suspected, MoGlo3 partially complements the function of yeast Glo3p. Consistent with findings in S. cerevisiae, MoGlo3 is localized to the Golgi, and that the localization is dependent on the conserved BoCCS domain. We found that MoGlo3 is highly expressed during conidiation and early infection stages and is required for vegetative growth, conidial production and sexual development. We further found that the ΔMoglo3 mutant is defective in endocytosis, scavenging of the reactive oxygen species, and in the response to endoplasmic reticulum (ER) stress. The combined effects result in failed appressorium function and decreased pathogenicity. Moreover, we provided evidence showing that the domains including the GAP, BoCCS and GRM are all important for normal MoGlo3 functions. Our studies further illustrate the importance of normal membrane trafficking in the physiology and pathogenicity of the rice blast fungus.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Zhang S,Liu X,Li L,Yu R,He J,Zhang H,Zheng X,Wang P,Zhang Zdoi
10.1111/1462-2920.13798subject
Has Abstractpub_date
2017-10-01 00:00:00pages
3982-3996issue
10eissn
1462-2912issn
1462-2920journal_volume
19pub_type
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journal_title:Environmental microbiology
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journal_title:Environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/1462-2920.14728
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/1462-2920.13678
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pub_type: 杂志文章
doi:10.1111/1462-2920.12082
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journal_title:Environmental microbiology
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pub_type: 杂志文章
doi:10.1111/1462-2920.13056
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pub_type: 杂志文章
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journal_title:Environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Environmental microbiology
pub_type: 杂志文章
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journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13650
更新日期:2017-03-01 00:00:00