Design and validation of 16S rRNA probes to enumerate members of the Clostridium leptum subgroup in human faecal microbiota.

Abstract:

:Among human faecal bacteria, many members of the Clostridium leptum subgroup are fibrolytic and butyrate producing microorganisms thereby contributing to processes important to colonic health. Yet this phylogenetic subgroup remains poorly described to date. To improve detection and description of members of the C. leptum subgroup, the Clep 866 group probe was developed. Its association with probes targeting the Clostridium viride cluster (Cvir 1414) and Eubacterium desmolans species (Edes 635) allowed for the first time the detection of all members found in this phylogenetic group in human faecal microbiota. A species-specific probe was also designed to detect members of the Ruminococcus callidus species (Rcal 733). The design of signature regions was based on alignment of 16S rRNA sequences isolated from faeces of five healthy adults. Furthermore, an oligonucleotide competitor strategy was developed in order to improve the specificity of the probes formerly validated or designed in this study. The oligonucleotide probes were tested using a collection of target and non-target strains using FISH combined with flow cytometry. These new probes were added to a panel of 18 phylogenetic probes selected to describe faecal microbiota composition in 21 human faeces of healthy adults. Clostridium leptum subgroup represented 22% of the total faecal bacteria and codominated with members of Clostridium coccoides group. The cluster Faecalibacterium prausnitzii was the dominant component of the C. leptum subgroup and 20% of the latter subgroup remained unidentified at the species level.

journal_name

Environ Microbiol

authors

Lay C,Sutren M,Rochet V,Saunier K,Doré J,Rigottier-Gois L

doi

10.1111/j.1462-2920.2005.00763.x

keywords:

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

933-46

issue

7

eissn

1462-2912

issn

1462-2920

pii

EMI763

journal_volume

7

pub_type

杂志文章
  • Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing.

    abstract::It is well recognized that a dynamic biofilm develops upon plant biomass in the herbivore gastrointestinal tract, but this component of the microbiome has not previously been specifically sampled, or directly compared with the biodiversity present in the planktonic fraction of digesta. In this study, the digesta colle...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00721.x

    authors: Larue R,Yu Z,Parisi VA,Egan AR,Morrison M

    更新日期:2005-04-01 00:00:00

  • Phage-bacteria interaction network in human oral microbiome.

    abstract::Although increasing knowledge suggests that bacteriophages play important roles in regulating microbial ecosystems, phage-bacteria interaction in human oral cavities remains less understood. Here we performed a metagenomic analysis to explore the composition and variation of oral dsDNA phage populations and potential ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12923

    authors: Wang J,Gao Y,Zhao F

    更新日期:2016-07-01 00:00:00

  • Preferential occurrence of diazotrophic endophytes, Azoarcus spp., in wild rice species and land races of Oryza sativa in comparison with modern races.

    abstract::Several diazotrophic species of Azoarcus spp. occur as endophytes in the pioneer plant Kallar grass. The purpose of this study was to screen Asian wild rice and cultivated Oryza sativa varieties for natural association with these endophytes. Populations of culturable diazotrophs in surface-sterilized roots were charac...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2000.00078.x

    authors: Engelhard M,Hurek T,Reinhold-Hurek B

    更新日期:2000-04-01 00:00:00

  • Diverse seed banks favour adaptation of microalgal populations to future climate conditions.

    abstract::Selection of suitable genotypes from diverse seed banks may help phytoplankton populations to cope with environmental changes. This study examines whether the high genotypic diversity found in the Baltic cyst pool of the toxic dinoflagellate Alexandrium ostenfeldii is coupled to phenotypic variability that could aid s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13070

    authors: Kremp A,Oja J,LeTortorec AH,Hakanen P,Tahvanainen P,Tuimala J,Suikkanen S

    更新日期:2016-02-01 00:00:00

  • The geographical patterns of symbiont diversity in the invasive legume Mimosa pudica can be explained by the competitiveness of its symbionts and by the host genotype.

    abstract::Variations in the patterns of diversity of symbionts have been described worldwide on Mimosa pudica, a pan-tropical invasive species that interacts with both α and β-rhizobia. In this study, we investigated if symbiont competitiveness can explain these variations and the apparent prevalence of β- over α-rhizobia. We d...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12286

    authors: Melkonian R,Moulin L,Béna G,Tisseyre P,Chaintreuil C,Heulin K,Rezkallah N,Klonowska A,Gonzalez S,Simon M,Chen WM,James EK,Laguerre G

    更新日期:2014-07-01 00:00:00

  • Seasonality and monthly dynamics of marine myovirus communities.

    abstract::Marine myoviruses (i.e. bacteriophages with a contractile tail sheath) are numerically abundant and genetically diverse. We developed a terminal restriction fragment length polymorphism assay (TRFLP) for g23, the conserved gene encoding the major capsid protein, to investigate T4-like myovirus communities at USC's Mic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02744.x

    authors: Chow CE,Fuhrman JA

    更新日期:2012-08-01 00:00:00

  • A wide diversity of previously undetected free-living relatives of diplomonads isolated from marine/saline habitats.

    abstract::Over the last 15 years classical culturing and environmental PCR techniques have revealed a modest number of genuinely new major lineages of protists; however, some new groups have greatly influenced our understanding of eukaryote evolution. We used culturing techniques to examine the diversity of free-living protists...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02239.x

    authors: Kolisko M,Silberman JD,Cepicka I,Yubuki N,Takishita K,Yabuki A,Leander BS,Inouye I,Inagaki Y,Roger AJ,Simpson AG

    更新日期:2010-10-01 00:00:00

  • Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.

    abstract::In this study, the putative role of phenazines and rhamnolipid-biosurfactants, antagonistic metabolites produced by Pseudomonas aeruginosa PNA1, was tested in the biological control of Pythium splendens on bean (Phaseolus vulgaris L) and Pythium myriotylum on cocoyam (Xanthosoma sagittifolium L Schott). A rhamnolipid-...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01501.x

    authors: Perneel M,D'hondt L,De Maeyer K,Adiobo A,Rabaey K,Höfte M

    更新日期:2008-03-01 00:00:00

  • Distribution of S-layers on the surface of Bacillus cereus strains: phylogenetic origin and ecological pressure.

    abstract::Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis have been described as members of the Bacillus cereus group but are, in fact, one species. B. anthracis is a mammal pathogen, B. thuringiensis an entomopathogen and B. cereus a ubiquitous soil bacterium and an occasional human pathogen. In two clinical iso...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00220.x

    authors: Mignot T,Denis B,Couture-Tosi E,Kolstø AB,Mock M,Fouet A

    更新日期:2001-08-01 00:00:00

  • The antitoxin MqsA homologue in Pseudomonas fluorescens 2P24 has a rewired regulatory circuit through evolution.

    abstract::The mqsRA operon encodes a toxin-antitoxin pair that was characterized to participate in biofilm and persister cell formation in Escherichia coli. Notably, the antitoxin MqsA possesses a C-terminal DNA-binding domain that recognizes the [5'-AACCT(N)2-4 AGGTT-3'] motif and acts as a transcriptional regulator controllin...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14538

    authors: Wang Y,Zhang SP,Zhang MY,Kempher ML,Guo DD,Han JT,Tao X,Wu Y,Zhang LQ,He YX

    更新日期:2019-05-01 00:00:00

  • MrpacC regulates sporulation, insect cuticle penetration and immune evasion in Metarhizium robertsii.

    abstract::pH-responsive transcription factor of the PacC/Rim101 family governs adaptation to environment, development and virulence in many fungal pathogens. In this study, we report the functions of a PacC homologue, MrpacC, in an insect pathogenic fungus Metarhizium robertsii. The gene was highly transcribed in the fungus in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12451

    authors: Huang W,Shang Y,Chen P,Gao Q,Wang C

    更新日期:2015-04-01 00:00:00

  • Colonization, penetration and transformation of manganese oxide nodules by Aspergillus niger.

    abstract::In this study, the ability of the geoactive fungus Aspergillus niger to colonize and transform manganese nodules from the Clarion-Clipperton Zone in both solid and liquid media was investigated. Aspergillus niger was able to colonize and penetrate manganese nodules embedded in solid medium and effect extensive transfo...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14591

    authors: Ferrier J,Yang Y,Csetenyi L,Gadd GM

    更新日期:2019-05-01 00:00:00

  • Evidence for selective bacterial community structuring on microplastics.

    abstract::In aquatic ecosystems, microplastics are a relatively new anthropogenic substrate that can readily be colonized by biofilm-forming organisms. To examine the effects of substrate type on microbial community assembly, we exposed ambient Baltic bacterioplankton to plastic substrates commonly found in marine environments ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14120

    authors: Ogonowski M,Motiei A,Ininbergs K,Hell E,Gerdes Z,Udekwu KI,Bacsik Z,Gorokhova E

    更新日期:2018-08-01 00:00:00

  • Sequential bioavailability of sedimentary organic matter to heterotrophic bacteria.

    abstract::Aquatic sediments harbour diverse microbial communities that mediate organic matter degradation and influence biogeochemical cycles. The pool of bioavailable carbon continuously changes as a result of abiotic processes and microbial activity. It remains unclear how microbial communities respond to heterogeneous organi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13745

    authors: Mahmoudi N,Beaupré SR,Steen AD,Pearson A

    更新日期:2017-07-01 00:00:00

  • Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low %G+C content.

    abstract::Bioactive amounts of antibiotics as well as resistant bacteria reach the soil through manure fertilization. We investigated plasmids that may stimulate the environmental spread and interspecies transfer of antibiotic resistance. After treatment of two soils with manure, either with or without the sulfonamide antibioti...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01819.x

    authors: Heuer H,Kopmann C,Binh CT,Top EM,Smalla K

    更新日期:2009-04-01 00:00:00

  • O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates.

    abstract::The O-antigen is a highly diverse structure expressed on the outer surface of Gram-negative bacteria. The products responsible for O-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. While heterogeneous O-antigens are observed within Vibrio species, characterization of these ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02274.x

    authors: Wildschutte H,Preheim SP,Hernandez Y,Polz MF

    更新日期:2010-11-01 00:00:00

  • Molecular complexity orchestrates modulation of phagosome biogenesis and escape to the cytosol of macrophages by Francisella tularensis.

    abstract::Upon entry of Francisella tularensis to macrophages, the Francisella-containing phagosome (FCP) is trafficked into an acidified late endosome-like phagosome with limited fusion to the lysosomes followed by rapid escape into the cytosol where the organism replicates. Although the Francisella Pathogenicity Island (FPI),...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02229.x

    authors: Asare R,Abu Kwaik Y

    更新日期:2010-09-01 00:00:00

  • The arbuscular mycorrhizal fungus Rhizophagus irregularis uses a reductive iron assimilation pathway for high-affinity iron uptake.

    abstract::Arbuscular mycorrhizal (AM) fungi can improve iron (Fe) acquisition of their host plants. Here, we report a characterization of two components of the high-affinity reductive Fe uptake system of Rhizophagus irregularis, the ferric reductase (RiFRE1) and the high affinity Fe permeases (RiFTR1-2). In the extraradical myc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14121

    authors: Tamayo E,Knight SAB,Valderas A,Dancis A,Ferrol N

    更新日期:2018-05-01 00:00:00

  • Shifts in microbial community composition and function in the acidification of a lead/zinc mine tailings.

    abstract::In an attempt to link the microbial community composition and function in mine tailings to the generation of acid mine drainage, we simultaneously explored the geochemistry and microbiology of six tailings collected from a lead/zinc mine, i.e. primary tailings (T1), slightly acidic tailings (T2), extremely acidic tail...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12114

    authors: Chen LX,Li JT,Chen YT,Huang LN,Hua ZS,Hu M,Shu WS

    更新日期:2013-09-01 00:00:00

  • In vitro and in vivo expression of virulence genes in Vibrio isolates belonging to the Harveyi clade in relation to their virulence towards gnotobiotic brine shrimp (Artemia franciscana).

    abstract::Vibrios belonging to the Harveyi clade are pathogenic marine bacteria affecting both vertebrates and invertebrates, thereby causing a severe threat to the aquaculture industry. In this study, the expression of haemolysin, metalloprotease, serine protease, the quorum sensing master regulator LuxR and the virulence regu...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02354.x

    authors: Ruwandeepika HA,Defoirdt T,Bhowmick PP,Karunasagar I,Karunasagar I,Bossier P

    更新日期:2011-02-01 00:00:00

  • Free Shiga toxin 1-encoding bacteriophages are less prevalent than Shiga toxin 2 phages in extraintestinal environments.

    abstract::Stx bacteriophages are involved in the pathogenicity of Stx-producing Escherichia coli. Induction of the Stx phage lytic cycle increases Stx expression and releases Stx phages that reach extracellular environments. Stx phage family comprises different phages that harbour any stx subtype. Stx2 is closely related with s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13053

    authors: Grau-Leal F,Quirós P,Martínez-Castillo A,Muniesa M

    更新日期:2015-11-01 00:00:00

  • Diversity and distribution of a key sulpholipid biosynthetic gene in marine microbial assemblages.

    abstract::Sulphoquinovosyldiacylglycerols (SQDG) are polar sulphur-containing membrane lipids, whose presence has been related to a microbial strategy to adapt to phosphate deprivation. In this study, we have targeted the sqdB gene coding the uridine 5'-diphosphate-sulphoquinovose (UDP-SQ) synthase involved in the SQDG biosynth...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12202

    authors: Villanueva L,Bale N,Hopmans EC,Schouten S,Damsté JS

    更新日期:2014-03-01 00:00:00

  • Assessing sub-seafloor microbial activity by combined stable isotope probing with deuterated water and 13C-bicarbonate.

    abstract::Sub-seafloor sediments are populated by large numbers of microbial cells but not much is known about their metabolic activities, growth rates and carbon assimilation pathways. Here we introduce a new method enabling the sensitive detection of microbial lipid production and the distinction of auto- and heterotrophic ca...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02739.x

    authors: Wegener G,Bausch M,Holler T,Thang NM,Prieto Mollar X,Kellermann MY,Hinrichs KU,Boetius A

    更新日期:2012-06-01 00:00:00

  • Low-diversity bacterial community in the gut of the fruitfly Drosophila melanogaster.

    abstract::The bacteria in the fruitfly Drosophila melanogaster of different life stages was quantified by 454 pyrosequencing of 16S rRNA gene amplicons. The sequence reads were dominated by 5 operational taxonomic units (OTUs) at ≤ 97% sequence identity that could be assigned to Acetobacter pomorum, A. tropicalis, Lactobacillus...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02511.x

    authors: Wong CN,Ng P,Douglas AE

    更新日期:2011-07-01 00:00:00

  • Quantitative PCR reveals transient and persistent algal viruses in Lake Ontario, Canada.

    abstract::To determine if different algal viruses (Phycodnaviridae) share common patterns of seasonal abundance, quantitative PCR methods were developed and applied to monitor the abundances of three different viruses in Lake Ontario, Canada over 13 months. Throughout the year, the abundances of two different phycodnavirus polB...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01988.x

    authors: Short SM,Short CM

    更新日期:2009-10-01 00:00:00

  • Novel Caudovirales associated with Marine Group I Thaumarchaeota assembled from metagenomes.

    abstract::Marine Group I (MGI) Thaumarchaeota are some of the most abundant microorganisms in the deep ocean and responsible for much of the ammonia oxidation occurring in this environment. In this work, we present 35 sequences assembled from metagenomic samples of the first uncultivated Caudovirales viruses associated with Tha...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14462

    authors: López-Pérez M,Haro-Moreno JM,de la Torre JR,Rodriguez-Valera F

    更新日期:2019-06-01 00:00:00

  • Links between ammonia oxidizer species composition, functional diversity and nitrification kinetics in grassland soils.

    abstract::Molecular approaches have revealed considerable diversity and uncultured novelty in natural prokaryotic populations, but not direct links between the new genotypes detected and ecosystem processes. Here we describe the influence of the structure of communities of ammonia-oxidizing bacteria on nitrogen cycling in micro...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00740.x

    authors: Webster G,Embley TM,Freitag TE,Smith Z,Prosser JI

    更新日期:2005-05-01 00:00:00

  • Diversity, relative abundance and metabolic potential of bacterial endosymbionts in three Bathymodiolus mussel species from cold seeps in the Gulf of Mexico.

    abstract::Cold seeps in the Gulf of Mexico are often dominated by mussels of the genus Bathymodiolus that harbour symbiotic bacteria in their gills. In this study, we analysed symbiont diversity, abundance and metabolic potential in three mussel species from the northern Gulf of Mexico: Bathymodiolus heckerae from the West Flor...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01259.x

    authors: Duperron S,Sibuet M,MacGregor BJ,Kuypers MM,Fisher CR,Dubilier N

    更新日期:2007-06-01 00:00:00

  • A molecular biological protocol to distinguish potentially human pathogenic Stenotrophomonas maltophilia from plant-associated Stenotrophomonas rhizophila.

    abstract::In recent years, the importance of the Gram-negative bacterium Stenotrophomonas as an opportunistic pathogen as well as in biotechnology has increased. The aim of the present study was to develop new methods for distinguishing between strains closely related to the potentially human pathogenic Stenotrophomonas maltoph...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00928.x

    authors: Ribbeck-Busch K,Roder A,Hasse D,de Boer W,Martínez JL,Hagemann M,Berg G

    更新日期:2005-11-01 00:00:00

  • Elucidation of sevadicin, a novel non-ribosomal peptide secondary metabolite produced by the honey bee pathogenic bacterium Paenibacillus larvae.

    abstract::American foulbrood (AFB) caused by the bee pathogenic bacterium Paenibacillus larvae is the most devastating bacterial disease of honey bees worldwide. From AFB-dead larvae, pure cultures of P. larvae can normally be cultivated indicating that P. larvae is able to defend its niche against all other bacteria present. R...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12417

    authors: Garcia-Gonzalez E,Müller S,Ensle P,Süssmuth RD,Genersch E

    更新日期:2014-05-01 00:00:00