Insights into the antibiotic resistance dissemination in a wastewater effluent microbiome: bacteria, viruses and vesicles matter.

Abstract:

:Wastewater treatment plants effluents are considered as hotspots for the dispersion of antibiotic resistance genes (ARGs) into natural ecosystems. The bacterial resistome (ARG collection in a metagenome) analyses have provided clues on antibacterial resistance dynamics. However, viruses and vesicles are frequently ignored. Here, we addressed the bacterial, viral and vesicle resistomes from a representative wastewater effluent in natural conditions and amended with polymyxin, which is used as a last resort antibiotic. Metagenomics showed that the natural prokaryotic resistome was vast (9000 ARG hits/Gb metagenome) and diverse, while viral resistome was two orders of magnitude lower (50 ARG hits/Gb metagenome) suggesting that viruses rarely encoded ARGs. After polymyxin amendment, data showed no ARG enrichment - including to polymyxin - in the microbiome. Remarkably, microbiomes responded to polymyxin with a vast release of putative vesicles (threefold increase compared with the control), which might be used as 'traps' to decrease the antibiotic concentration. Intriguingly, although polymyxin resistance genes (PRGs) were rare in the microbiome (0.018% of total ARG found), in the viral and vesicle fractions, PRGs were more abundant (0.5%-0.8% of total ARG found). Our data suggest that vesicles could have a more active role in the context of transmission of antibiotic resistances.

journal_name

Environ Microbiol

authors

Maestre-Carballa L,Lluesma Gomez M,Angla Navarro A,Garcia-Heredia I,Martinez-Hernandez F,Martinez-Garcia M

doi

10.1111/1462-2920.14758

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

4582-4596

issue

12

eissn

1462-2912

issn

1462-2920

journal_volume

21

pub_type

杂志文章
  • Effects of diurnal temperature variation on microbial community and petroleum hydrocarbon biodegradation in contaminated soils from a sub-Arctic site.

    abstract::Contaminated soils are subject to diurnal and seasonal temperature variations during on-site ex-situ bioremediation processes. We assessed how diurnal temperature variations similar to that in summer at the site from which petroleum hydrocarbon-contaminated soil was collected affect the soil microbial community and th...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12846

    authors: Akbari A,Ghoshal S

    更新日期:2015-12-01 00:00:00

  • Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly.

    abstract::Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high-resolution small subunit rRNA gene sequencing to explore archaeal diversity in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15058

    authors: Juottonen H,Fontaine L,Wurzbacher C,Drakare S,Peura S,Eiler A

    更新日期:2020-08-01 00:00:00

  • Metabolic versatility of a novel N2 -fixing Alphaproteobacterium isolated from a marine oxygen minimum zone.

    abstract::The N2 -fixing (diazotrophic) community in marine ecosystems is dominated by non-cyanobacterial microorganisms. Yet, very little is known about their identity, function and ecological relevance due to a lack of cultured representatives. Here we report a novel heterotrophic diazotroph isolated from the oxygen minimum z...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14008

    authors: Martínez-Pérez C,Mohr W,Schwedt A,Dürschlag J,Callbeck CM,Schunck H,Dekaezemacker J,Buckner CRT,Lavik G,Fuchs BM,Kuypers MMM

    更新日期:2018-02-01 00:00:00

  • Novel Caudovirales associated with Marine Group I Thaumarchaeota assembled from metagenomes.

    abstract::Marine Group I (MGI) Thaumarchaeota are some of the most abundant microorganisms in the deep ocean and responsible for much of the ammonia oxidation occurring in this environment. In this work, we present 35 sequences assembled from metagenomic samples of the first uncultivated Caudovirales viruses associated with Tha...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14462

    authors: López-Pérez M,Haro-Moreno JM,de la Torre JR,Rodriguez-Valera F

    更新日期:2019-06-01 00:00:00

  • Evolving metabolism of 2,4-dinitrotoluene triggers SOS-independent diversification of host cells.

    abstract::The molecular mechanisms behind the mutagenic effect of reactive oxygen species (ROS) released by defective metabolization of xenobiotic 2,4-dinitrotoluene (DNT) by a still-evolving degradation pathway were studied. To this end, the genes required for biodegradation of DNT from Burkholderia cepacia R34 were implanted ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14459

    authors: Akkaya Ö,Nikel PI,Pérez-Pantoja D,de Lorenzo V

    更新日期:2019-01-01 00:00:00

  • Ectomycorrhizal fungi inoculation alleviates simulated acid rain effects on soil ammonia oxidizers and denitrifiers in Masson pine forest.

    abstract::Acid rain can cause severe effects on soil biota and nutrient biogeochemical cycles in the forest ecosystem, but how plant-symbiotic ectomycorrhizal fungi will modulate the effects remains unknown. Here, we conducted a full factorial field experiment in a Masson pine forest by simultaneously controlling the acidity of...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14457

    authors: Li Y,Chen Z,He JZ,Wang Q,Shen C,Ge Y

    更新日期:2019-01-01 00:00:00

  • Cyanobacterial distributions along a physico-chemical gradient in the Northeastern Pacific Ocean.

    abstract::The cyanobacteria Prochlorococcus and Synechococcus are important marine primary producers. We explored their distributions and covariance along a physico-chemical gradient from coastal to open ocean waters in the Northeastern Pacific Ocean. An inter-annual pattern was delineated in the dynamic transition zone where u...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12742

    authors: Sudek S,Everroad RC,Gehman AL,Smith JM,Poirier CL,Chavez FP,Worden AZ

    更新日期:2015-10-01 00:00:00

  • Transcription and mass-spectroscopic proteomic studies of electron transport oxidoreductases in Dehalococcoides ethenogenes.

    abstract::Besides 19 potential reductive dehalogenase genes, the genome of Dehalococcoides ethenogenes strain 195 contains over 60 genes annotated as encoding oxidoreductases, including five hydrogenase complexes and a formate dehydrogenase (Fdh). Using quantitative reverse transcriptase polymerase chain reaction, we found that...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01090.x

    authors: Morris RM,Sowell S,Barofsky D,Zinder S,Richardson R

    更新日期:2006-09-01 00:00:00

  • Comparison of coastal phytoplankton composition estimated from the V4 and V9 regions of the 18S rRNA gene with a focus on photosynthetic groups and especially Chlorophyta.

    abstract::We compared the composition of eukaryotic communities using two genetic markers (18S rRNA V4 and V9 regions) at 27 sites sampled during Ocean Sampling Day 2014, with a focus on photosynthetic groups and, more specifically green algae (Chlorophyta). Globally, the V4 and V9 regions of the 18S rRNA gene provided similar ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13952

    authors: Tragin M,Zingone A,Vaulot D

    更新日期:2018-02-01 00:00:00

  • Persister cells resuscitate via ribosome modification by 23S rRNA pseudouridine synthase RluD.

    abstract::Upon a wide range of stress conditions (e.g. nutrient, antibiotic, oxidative), a subpopulation of bacterial cells known as persisters survives by halting metabolism. These cells resuscitate rapidly to reconstitute infections once the stress is removed and nutrients are provided. However, how these dormant cells resusc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14828

    authors: Song S,Wood TK

    更新日期:2020-03-01 00:00:00

  • Functional and structural response of a cellulose-degrading methanogenic microbial community to multiple aeration stress at two different temperatures.

    abstract::Two cellulose-fermenting methanogenic enrichment cultures originating from rice soil, one at 15 degrees C with Methanosaeta and the other at 30 degrees C with Methanosarcina as the dominant acetoclastic methanogen, both degraded cellulose anaerobically via propionate, acetate and H2 to CH4. The degradation was a two-s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00199.x

    authors: Wu XL,Conrad R

    更新日期:2001-06-01 00:00:00

  • High bacterial diversity of a waste gas-degrading community in an industrial biofilter as shown by a 16S rDNA clone library.

    abstract::The bacterial diversity of an industrial biofilter used for waste gas abatement in an animal-rendering plant was investigated. A 16S rDNA clone library was generated and 444 clones were screened using computer-aided amplified ribosomal DNA restriction analysis (ARDRA). Of the screened clones, 60.8% showed unique ARDRA...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00349.x

    authors: Friedrich U,Prior K,Altendorf K,Lipski A

    更新日期:2002-11-01 00:00:00

  • Replication, lies and lesser-known truths regarding experimental design in environmental microbiology.

    abstract::A recent analysis revealed that most environmental microbiologists neglect replication in their science (Prosser, 2010). Of all peer-reviewed papers published during 2009 in the field's leading journals, slightly more than 70% lacked replication when it came to analyzing microbial community data. The paucity of replic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02445.x

    authors: Lennon JT

    更新日期:2011-06-01 00:00:00

  • Off-axis symbiosis found: Characterization and biogeography of bacterial symbionts of Bathymodiolus mussels from Lost City hydrothermal vents.

    abstract::Organisms at hydrothermal vents inhabit discontinuous chemical 'islands' along mid-ocean ridges, a scenario that may promote genetic divergence among populations. The 2003 discovery of mussels at the Lost City Hydrothermal Field provided a means of evaluating factors that govern the biogeography of symbiotic bacteria ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.01113.x

    authors: DeChaine EG,Bates AE,Shank TM,Cavanaugh CM

    更新日期:2006-11-01 00:00:00

  • Uptake-release dynamics of the inorganic and organic carbon pool mediated by planktonic prokaryotes in the deep Mediterranean Sea.

    abstract::Understanding the ecosystem functioning in the dark portion of the ocean is a challenge that microbial ecologists are still facing. Due to the large volume, the global deep Ocean plays a central role in the regulation of climate, possibly buffering the rise of atmospheric carbon dioxide if processes of CO2 fixation co...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13641

    authors: Celussi M,Malfatti F,Ziveri P,Giani M,Del Negro P

    更新日期:2017-03-01 00:00:00

  • Subsistence and complexity of antimicrobial resistance on a community-wide level.

    abstract::There are a multitude of resistance strategies that microbes can apply to avoid inhibition by antimicrobials. One of these strategies is the enzymatic modification of the antibiotic, in a process generally termed inactivation. Furthermore, some microorganisms may not be limited to the mere inactivation of the antimicr...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15018

    authors: Perri R,Kolvenbach BA,Corvini PFX

    更新日期:2020-07-01 00:00:00

  • Early rhizosphere microbiome composition is related to the growth and Zn uptake of willows introduced to a former landfill.

    abstract::Although plants introduced for site restoration are pre-selected for specific traits (e.g. trace element bioaccumulation, rapid growth in poor soils), the in situ success of these plants likely depends on the recruitment of appropriate rhizosphere microorganisms from their new environment. We introduced three willow (...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12900

    authors: Bell TH,Cloutier-Hurteau B,Al-Otaibi F,Turmel MC,Yergeau E,Courchesne F,St-Arnaud M

    更新日期:2015-08-01 00:00:00

  • Catalases play differentiated roles in the adaptation of a fungal entomopathogen to environmental stresses.

    abstract::The catalase family of Beauveria bassiana (fungal entomopathogen) consists of catA (spore-specific), catB (secreted), catP (peroxisomal), catC (cytoplasmic) and catD (secreted peroxidase/catalase), which were distinguished in phylogeny and structure and functionally characterized by constructing single-gene disrupted ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02848.x

    authors: Wang ZL,Zhang LB,Ying SH,Feng MG

    更新日期:2013-02-01 00:00:00

  • Phylogenetic identification and metabolism of marine dimethylsulfide-consuming bacteria.

    abstract::Microbial consumption is one of the main processes, along with photolysis and ventilation, that remove the biogenic trace gas dimethylsulfide (DMS) from the surface ocean. Although a few isolates of marine bacteria have been studied for their ability to utilize DMS, little is known about the characteristics or phyloge...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01102.x

    authors: Vila-Costa M,Del Valle DA,González JM,Slezak D,Kiene RP,Sánchez O,Simó R

    更新日期:2006-12-01 00:00:00

  • Diversity of phytobeneficial traits revealed by whole-genome analysis of worldwide-isolated phenazine-producing Pseudomonas spp.

    abstract::Plant-beneficial Pseudomonas spp. competitively colonize the rhizosphere and display plant-growth promotion and/or disease-suppression activities. Some strains within the P. fluorescens species complex produce phenazine derivatives, such as phenazine-1-carboxylic acid. These antimicrobial compounds are broadly inhibit...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14476

    authors: Biessy A,Novinscak A,Blom J,Léger G,Thomashow LS,Cazorla FM,Josic D,Filion M

    更新日期:2019-01-01 00:00:00

  • The ArfGAP protein MoGlo3 regulates the development and pathogenicity of Magnaporthe oryzae.

    abstract::The ADP ribosylation factor (Arf) and the coat protein complex I (COPI) are involved in vesicle transport. Together with GTPase-activating proteins (ArfGAPs) and guanine exchange factors (ArfGEFs) that regulate the activity of Arf, they govern vesicle formation, COPI trafficking and the maintenance of the Golgi comple...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13798

    authors: Zhang S,Liu X,Li L,Yu R,He J,Zhang H,Zheng X,Wang P,Zhang Z

    更新日期:2017-10-01 00:00:00

  • Methanogenesis, sulfate reduction and crude oil biodegradation in hot Alaskan oilfields.

    abstract::Petrochemical and geological evidence suggest that petroleum in most reservoirs is anaerobically biodegraded to some extent. However, the conditions for this metabolism and the cultivation of the requisite microorganisms are rarely established. Here, we report on microbial hydrocarbon metabolism in two distinct oilfie...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02282.x

    authors: Gieg LM,Davidova IA,Duncan KE,Suflita JM

    更新日期:2010-11-01 00:00:00

  • Bacterial biogeography in the coastal waters of northern Zhejiang, East China Sea is highly controlled by spatially structured environmental gradients.

    abstract::The underlying mechanisms of microbial community assembly in connective coastal environments are unclear. The coastal water area of northern Zhejiang, East China Sea, is a complex marine ecosystem with multiple environmental gradients, where the distributions and determinants of bacterioplankton communities remain unc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12884

    authors: Wang K,Ye X,Chen H,Zhao Q,Hu C,He J,Qian Y,Xiong J,Zhu J,Zhang D

    更新日期:2015-10-01 00:00:00

  • Microbial anaerobic Fe(II) oxidation - Ecology, mechanisms and environmental implications.

    abstract::Iron is the most abundant redox-active metal in the Earth's crust. The one electron transfer between the two most common redox states, Fe(II) and Fe(III), plays a role in a huge range of environmental processes from mineral formation and dissolution to contaminant remediation and global biogeochemical cycling. It has ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.14328

    authors: Bryce C,Blackwell N,Schmidt C,Otte J,Huang YM,Kleindienst S,Tomaszewski E,Schad M,Warter V,Peng C,Byrne JM,Kappler A

    更新日期:2018-10-01 00:00:00

  • Diversity and distribution of a key sulpholipid biosynthetic gene in marine microbial assemblages.

    abstract::Sulphoquinovosyldiacylglycerols (SQDG) are polar sulphur-containing membrane lipids, whose presence has been related to a microbial strategy to adapt to phosphate deprivation. In this study, we have targeted the sqdB gene coding the uridine 5'-diphosphate-sulphoquinovose (UDP-SQ) synthase involved in the SQDG biosynth...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12202

    authors: Villanueva L,Bale N,Hopmans EC,Schouten S,Damsté JS

    更新日期:2014-03-01 00:00:00

  • Redox cycling of arsenic by the hydrothermal marine bacterium Marinobacter santoriniensis.

    abstract::Marinobacter santoriniensis NKSG1(T) is a mesophilic, dissimilatory arsenate-reducing and arsenite-oxidizing bacterium isolated from an arsenate-reducing enrichment culture. The inoculum was obtained from arsenic-rich shallow marine hydrothermal sediment from Santorini, Greece, with evidence of arsenic redox cycling. ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01890.x

    authors: Handley KM,Héry M,Lloyd JR

    更新日期:2009-06-01 00:00:00

  • Adaptive evolution of a melanized fungus reveals robust augmentation of radiation resistance by abrogating non-homologous end-joining.

    abstract::Fungi have been observed to exhibit resistance to high levels of ionizing radiation despite sharing most DNA repair mechanisms with other eukaryotes. Radioresistance, in fact, is such a common feature in fungi that it is difficult to identify species that exhibit widely different radiosensitivities, which in turn has ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15285

    authors: Romsdahl J,Schultzhaus Z,Chen A,Liu J,Ewing A,Hervey J,Wang Z

    更新日期:2020-10-19 00:00:00

  • Iron stress genes in marine Synechococcus and the development of a flow cytometric iron stress assay.

    abstract::Marine Synechococcus are frequently found in environments where iron (Fe) is a limiting nutrient. To understand their capacity to respond to Fe stress, we screened picoplankton genomes and the Global Ocean Survey metagenome for known Fe stress genes. Many open ocean strains of Synechococcus lack most known genes for F...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01778.x

    authors: Rivers AR,Jakuba RW,Webb EA

    更新日期:2009-02-01 00:00:00

  • Divinyl chlorophyll a in the marine eukaryotic protist Alexandrium ostenfeldii (Dinophyceae).

    abstract::Here it is reported the first detection of DV-chl a together with the usual chl a in the marine dinoflagellate Alexandrium ostenfeldii from the Baltic Sea. Growth response and photosynthetic parameters were examined at two irradiances (80 and 240 μmol photons m(-2) s(-1)) and temperatures (15 °C and 19 °C) in a diviny...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13042

    authors: Rodríguez F,Garrido JL,Sobrino C,Johnsen G,Riobó P,Franco J,Aamot I,Ramilo I,Sanz N,Kremp A

    更新日期:2016-02-01 00:00:00

  • HprKXcc is a serine kinase that regulates virulence in the Gram-negative phytopathogen Xanthomonas campestris.

    abstract::The HprK serine kinase is a component of the phosphoenolpyruvate phosphotransferase system (PTS) of bacteria that generally regulates catabolite repression through phosphorylation/dephosphorylation of the PTS protein PtsH at a conserved serine residue. However, many bacteria do not encode a complete PTS or even have a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14740

    authors: Li RF,Cui P,Wei PZ,Liu XY,Tang JL,Lu GT

    更新日期:2019-12-01 00:00:00