Evolving metabolism of 2,4-dinitrotoluene triggers SOS-independent diversification of host cells.

Abstract:

:The molecular mechanisms behind the mutagenic effect of reactive oxygen species (ROS) released by defective metabolization of xenobiotic 2,4-dinitrotoluene (DNT) by a still-evolving degradation pathway were studied. To this end, the genes required for biodegradation of DNT from Burkholderia cepacia R34 were implanted in Escherichia coli and the effect of catabolizing the nitroaromatic compound monitored with stress-related markers and reporters. Such a proxy of the naturally-occurring scenario faithfully recreated the known accumulation of ROS caused by faulty metabolism of DNT and the ensuing onset of an intense mutagenesis regime. While ROS triggered an oxidative stress response, neither homologous recombination was stimulated nor the recA promoter activity increased during DNT catabolism. Analysis of single-nucleotide changes occurring in rpoB during DNT degradation suggested a relaxation of DNA replication fidelity rather than direct damage to DNA. Mutants frequencies were determined in strains defective in either converting DNA damage into mutagenesis or mediating inhibition of mismatch repair through a general stress response. The results revealed that the mutagenic effect of ROS was largely SOS-independent and stemmed instead from stress-induced changes of rpoS functionality. Evolution of novel metabolic properties thus resembles the way sublethal antibiotic concentrations stimulate the appearance of novel resistance genes.

journal_name

Environ Microbiol

authors

Akkaya Ö,Nikel PI,Pérez-Pantoja D,de Lorenzo V

doi

10.1111/1462-2920.14459

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

314-326

issue

1

eissn

1462-2912

issn

1462-2920

journal_volume

21

pub_type

杂志文章
  • The presence of the glycolysis operon in SAR11 genomes is positively correlated with ocean productivity.

    abstract::Bacteria in the SAR11 clade are highly abundant in marine surface waters, but currently little is known about the carbon compounds that support these large heterotrophic populations. To better understand the carbon requirements of these organisms, we conducted a multiphasic exploration of carbohydrate utilization amon...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02092.x

    authors: Schwalbach MS,Tripp HJ,Steindler L,Smith DP,Giovannoni SJ

    更新日期:2010-02-01 00:00:00

  • The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina.

    abstract::Reactive oxygen species (ROS) play an important role during host-pathogen interactions and are often an indication of induced host defence responses. In this study, we demonstrate for the first time that Puccinia triticina (Pt) generates ROS, including superoxide, H2 O2 and hydroxyl radicals, during wheat infection. T...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15063

    authors: Wang X,Che MZ,Khalil HB,McCallum BD,Bakkeren G,Rampitsch C,Saville BJ

    更新日期:2020-07-01 00:00:00

  • Metabolic response of Agrobacterium tumefaciens 5A to arsenite.

    abstract::Wide-spread abundance in soil and water, coupled with high toxicity have put arsenic at the top of the list of environmental contaminants. Early studies demonstrated that both concentration and the valence state of inorganic arsenic (arsenite, As(III) vs. arsenate As(V)) can be modulated by microbes. Using genetics, t...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13615

    authors: Tokmina-Lukaszewska M,Shi Z,Tripet B,McDermott TR,Copié V,Bothner B,Wang G

    更新日期:2017-02-01 00:00:00

  • Global survey of diversity among environmental saltwater Bacteriovoracaceae.

    abstract::Halophilic Bacteriovorax (Bx), formerly known as the marine Bdellovibrio, are Gram-negative, predatory bacteria found in saltwater systems. To assess their genetic diversity and geographical occurrence, the small subunit rRNA (ssu-rRNA) gene sequences were analysed from 111 marine, salt lake and estuarine isolates rec...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01362.x

    authors: Pineiro SA,Stine OC,Chauhan A,Steyert SR,Smith R,Williams HN

    更新日期:2007-10-01 00:00:00

  • Does quorum sensing interference affect the fitness of bacterial pathogens in the real world?

    abstract::Many bacterial pathogens rely on quorum sensing to control virulence gene expression. Based on numerous experiments conducted under well-defined conditions, quorum sensing interference is considered as a promising strategy to tackle infections and thus might have the potential to (partially) replace antibiotics. Despi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.14446

    authors: Liu Y,Qin Q,Defoirdt T

    更新日期:2018-11-01 00:00:00

  • Transcriptional characterization of Vibrio fischeri during colonization of juvenile Euprymna scolopes.

    abstract::The marine bacterium Vibrio fischeri is the monospecific symbiont of the Hawaiian bobtail squid, Euprymna scolopes, and the establishment of this association involves a number of signaling pathways and transcriptional responses between both partners. We report here the first full RNA-Seq dataset representing host-asso...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13684

    authors: Thompson LR,Nikolakakis K,Pan S,Reed J,Knight R,Ruby EG

    更新日期:2017-05-01 00:00:00

  • Physiological and molecular genetic evaluation of the dechlorination agent, pyridine-2,6-bis(monothiocarboxylic acid) (PDTC) as a secondary siderophore of Pseudomonas.

    abstract::The bacterial metabolite and transition metal chelator pyridine-2,6-dithiocarboxylic acid (PDTC), promotes a novel and effective means of dechlorination of the toxic and carcinogenic pollutant, carbon tetrachloride. Pyridine-2,6-dithiocarboxylic acid has been presumed to act as a siderophore in the Pseudomonas strains...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2003.00558.x

    authors: Lewis TA,Leach L,Morales S,Austin PR,Hartwell HJ,Kaplan B,Forker C,Meyer JM

    更新日期:2004-02-01 00:00:00

  • Protein glycosylation as an adaptive response in Archaea: growth at different salt concentrations leads to alterations in Haloferax volcanii S-layer glycoprotein N-glycosylation.

    abstract::To cope with life in hypersaline environments, halophilic archaeal proteins are enriched in acidic amino acids. This strategy does not, however, offer a response to transient changes in salinity, as would post-translational modifications. To test this hypothesis, N-glycosylation of the Haloferax volcanii S-layer glyco...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02625.x

    authors: Guan Z,Naparstek S,Calo D,Eichler J

    更新日期:2012-03-01 00:00:00

  • Microbial diversity in ikaite tufa columns: an alkaline, cold ecological niche in Greenland.

    abstract::Ikaite tufa columns from the Ikka Fjord in south-western Greenland constitute a natural, stable environment at low temperature and with a pH ranging from neutral at the exterior to very alkaline (pH 10.4) at the interior of the column. Phylogenetic analysis of culturable organisms revealed ten different isolates repre...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00327.x

    authors: Stougaard P,Jørgensen F,Johnsen MG,Hansen OC

    更新日期:2002-08-01 00:00:00

  • Comparative proteomics of the toxigenic diazotroph Raphidiopsis raciborskii (cyanobacteria) in response to iron.

    abstract::Raphidiopsis raciborskii is an invasive bloom-forming cyanobacteria with the flexibility to utilize atmospheric and fixed nitrogen. Since nitrogen-fixation has a high requirement for iron as an ezyme cofactor, we hypothesize that iron availability would determine the success of the species under nitrogen-fixing condit...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15328

    authors: D'Agostino PM,Yeung ACY,Poljak A,David Waite T,Neilan BA

    更新日期:2021-01-01 00:00:00

  • Prophages of the genus Bifidobacterium as modulating agents of the infant gut microbiota.

    abstract::Phage predation is one of the key forces that shape genetic diversity in bacterial genomes. Phages are also believed to act as modulators of the microbiota composition and, consequently, as agents that drive bacterial speciation in complex bacterial communities. Very little is known about the occurrence and genetic va...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13154

    authors: Lugli GA,Milani C,Turroni F,Tremblay D,Ferrario C,Mancabelli L,Duranti S,Ward DV,Ossiprandi MC,Moineau S,van Sinderen D,Ventura M

    更新日期:2016-07-01 00:00:00

  • Nitrate-dependent iron(II) oxidation in paddy soil.

    abstract::Iron(III) profiles of flooded paddy soil incubated in the greenhouse indicated oxidation of iron(II) in the upper 6 mm soil layer. Measurement of oxygen with a Clark-type microelectrode showed that oxygen was only responsible for the oxidation of iron(II) in the upper 3 mm. In the soil beneath, nitrate could be used a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00163.x

    authors: Ratering S,Schnell S

    更新日期:2001-02-01 00:00:00

  • Seasonal patterns in microbial communities inhabiting the hot springs of Tengchong, Yunnan Province, China.

    abstract::Studies focusing on seasonal dynamics of microbial communities in terrestrial and marine environments are common; however, little is known about seasonal dynamics in high-temperature environments. Thus, our objective was to document the seasonal dynamics of both the physicochemical conditions and the microbial communi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12311

    authors: Briggs BR,Brodie EL,Tom LM,Dong H,Jiang H,Huang Q,Wang S,Hou W,Wu G,Huang L,Hedlund BP,Zhang C,Dijkstra P,Hungate BA

    更新日期:2014-06-01 00:00:00

  • Antioxidant-related catalase CTA1 regulates development, aflatoxin biosynthesis, and virulence in pathogenic fungus Aspergillus flavus.

    abstract::Reactive oxygen species (ROS) induce the synthesis of a myriad of secondary metabolites, including aflatoxins. It raises significant concern as it is a potent environmental contaminant. In Aspergillus flavus., antioxidant enzymes link ROS stress response with coordinated gene regulation of aflatoxin biosynthesis. In t...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15011

    authors: Zhu Z,Yang M,Bai Y,Ge F,Wang S

    更新日期:2020-07-01 00:00:00

  • Pyomelanin-producing Pseudomonas aeruginosa selected during chronic infections have a large chromosomal deletion which confers resistance to pyocins.

    abstract::When bacterial lineages make the transition from free-living to permanent association with hosts, they can undergo massive gene losses, for which the selective forces within host tissues are unknown. We identified here melanogenic clinical isolates of Pseudomonas aeruginosa with large chromosomal deletions (66 to 270 ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13336

    authors: Hocquet D,Petitjean M,Rohmer L,Valot B,Kulasekara HD,Bedel E,Bertrand X,Plésiat P,Köhler T,Pantel A,Jacobs MA,Hoffman LR,Miller SI

    更新日期:2016-10-01 00:00:00

  • Genome-wide identification of pathogenicity, conidiation and colony sectorization genes in Metarhizium robertsii.

    abstract::Metarhizium robertsii occupies a wide array of ecological niches and has diverse lifestyle options (saprophyte, insect pathogen and plant symbiont), that renders it an unusually effective model for studying genetic mechanisms for fungal adaptation. Here over 20,000 M. robertsii T-DNA mutants were screened in order to ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13777

    authors: Zeng G,Chen X,Zhang X,Zhang Q,Xu C,Mi W,Guo N,Zhao H,You Y,Dryburgh FJ,Bidochka MJ,St Leger RJ,Zhang L,Fang W

    更新日期:2017-10-01 00:00:00

  • Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples.

    abstract::Microbial community analysis via high-throughput sequencing of amplified 16S rRNA genes is an essential microbiology tool. We found the popular primer pair 515F (515F-C) and 806R greatly underestimated (e.g. SAR11) or overestimated (e.g. Gammaproteobacteria) common marine taxa. We evaluated marine samples and mock com...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13023

    authors: Parada AE,Needham DM,Fuhrman JA

    更新日期:2016-05-01 00:00:00

  • Low-diversity bacterial community in the gut of the fruitfly Drosophila melanogaster.

    abstract::The bacteria in the fruitfly Drosophila melanogaster of different life stages was quantified by 454 pyrosequencing of 16S rRNA gene amplicons. The sequence reads were dominated by 5 operational taxonomic units (OTUs) at ≤ 97% sequence identity that could be assigned to Acetobacter pomorum, A. tropicalis, Lactobacillus...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02511.x

    authors: Wong CN,Ng P,Douglas AE

    更新日期:2011-07-01 00:00:00

  • Hexavalent uranium supports growth of Anaeromyxobacter dehalogenans and Geobacter spp. with lower than predicted biomass yields.

    abstract::The stimulation of bacteria capable of reducing soluble U(VI) to sparingly soluble U(IV) is a promising approach for containing U(VI) plumes. Anaeromyxobacter dehalogenans is capable of mediating this activity; however, its ability to couple U(VI) reduction to growth has not been established. Monitoring the increase i...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01405.x

    authors: Sanford RA,Wu Q,Sung Y,Thomas SH,Amos BK,Prince EK,Löffler FE

    更新日期:2007-11-01 00:00:00

  • Molecular complexity orchestrates modulation of phagosome biogenesis and escape to the cytosol of macrophages by Francisella tularensis.

    abstract::Upon entry of Francisella tularensis to macrophages, the Francisella-containing phagosome (FCP) is trafficked into an acidified late endosome-like phagosome with limited fusion to the lysosomes followed by rapid escape into the cytosol where the organism replicates. Although the Francisella Pathogenicity Island (FPI),...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02229.x

    authors: Asare R,Abu Kwaik Y

    更新日期:2010-09-01 00:00:00

  • Multidimensional NMR analysis reveals truncated lignin structures in wood decayed by the brown rot basidiomycete Postia placenta.

    abstract::Lignocellulose biodegradation, an essential step in terrestrial carbon cycling, generally involves removal of the recalcitrant lignin barrier that otherwise prevents infiltration by microbial polysaccharide hydrolases. However, fungi that cause brown rot of wood, a major route for biomass recycling in coniferous fores...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02417.x

    authors: Yelle DJ,Wei D,Ralph J,Hammel KE

    更新日期:2011-04-01 00:00:00

  • Nitrogen fixation by a marine non-heterocystous cyanobacterium requires a heterotrophic bacterial consort.

    abstract::Cultures of the non-heterocystous cyanobacterium, Leptolyngbya nodulosa, could be grown indefinitely in media devoid of combined nitrogen. Acetylene reduction assays showed that these cultures fixed nitrogen in the dark period of a diurnal cycle under micro-oxygenic or anaerobic conditions. Addition of DCMU to culture...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02159.x

    authors: Li Z,Yu J,Kim KR,Brand J

    更新日期:2010-05-01 00:00:00

  • Wide diversity of parasites in Bombus terrestris (Linnaeus, 1758) revealed by a high-throughput sequencing approach.

    abstract::Assessing the extent of parasite diversity requires the application of appropriate molecular tools, especially given the growing evidence of multiple parasite co-occurrence. Here, we compared the performance of a next-generation sequencing technology (Ion PGM ™ System) in 12 Bombus terrestris specimens that were PCR-i...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15336

    authors: Bartolomé C,Jabal-Uriel C,Buendía-Abad M,Benito M,Ornosa C,De la Rúa P,Martín-Hernández R,Higes M,Maside X

    更新日期:2021-01-01 00:00:00

  • Early rhizosphere microbiome composition is related to the growth and Zn uptake of willows introduced to a former landfill.

    abstract::Although plants introduced for site restoration are pre-selected for specific traits (e.g. trace element bioaccumulation, rapid growth in poor soils), the in situ success of these plants likely depends on the recruitment of appropriate rhizosphere microorganisms from their new environment. We introduced three willow (...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12900

    authors: Bell TH,Cloutier-Hurteau B,Al-Otaibi F,Turmel MC,Yergeau E,Courchesne F,St-Arnaud M

    更新日期:2015-08-01 00:00:00

  • Diversity and abundance of freshwater Actinobacteria along environmental gradients in the brackish northern Baltic Sea.

    abstract::Actinobacteria are highly abundant in pelagic freshwater habitats and also occur in estuarine environments such as the Baltic Sea. Because of gradients in salinity and other environmental variables estuaries offer natural systems for examining factors that determine Actinobacteria distribution. We studied abundance an...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01925.x

    authors: Holmfeldt K,Dziallas C,Titelman J,Pohlmann K,Grossart HP,Riemann L

    更新日期:2009-08-01 00:00:00

  • O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates.

    abstract::The O-antigen is a highly diverse structure expressed on the outer surface of Gram-negative bacteria. The products responsible for O-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. While heterogeneous O-antigens are observed within Vibrio species, characterization of these ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02274.x

    authors: Wildschutte H,Preheim SP,Hernandez Y,Polz MF

    更新日期:2010-11-01 00:00:00

  • Competition between microcystin- and non-microcystin-producing Planktothrix agardhii (cyanobacteria) strains under different environmental conditions.

    abstract::The factors that control the production of microcystins (hepatotoxins) during cyanobacterial blooms, and the function of these metabolites remain largely unknown. In an attempt to provide answers to these questions, we compared the fitness of microcystin (MC)-producing and non-MC-producing Planktothrix agardhii strain...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01730.x

    authors: Briand E,Yéprémian C,Humbert JF,Quiblier C

    更新日期:2008-12-01 00:00:00

  • Geomicrobiological exploration and characterization of a novel deep-sea hydrothermal system at the TOTO caldera in the Mariana Volcanic Arc.

    abstract::Novel hydrothermal activities accompanying effluent white smokers and elemental sulfur chimney structures at the north-east lava dome of the TOTO caldera depression in the Mariana Volcanic Arc have been explored and characterized by geochemical and microbiological surveys. White smoker hydrothermal fluids were observe...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00884.x

    authors: Nakagawa T,Takai K,Suzuki Y,Hirayama H,Konno U,Tsunogai U,Horikoshi K

    更新日期:2006-01-01 00:00:00

  • Dilution cultivation of marine heterotrophic bacteria abundant after a spring phytoplankton bloom in the North Sea.

    abstract::The roles of individual bacterioplankton species in the re-mineralization of algal biomass are poorly understood. Evidence from molecular data had indicated that a spring diatom bloom in the German Bight of the North Sea in 2009 was followed by a rapid succession of uncultivated bacterioplankton species, including mem...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12479

    authors: Hahnke RL,Bennke CM,Fuchs BM,Mann AJ,Rhiel E,Teeling H,Amann R,Harder J

    更新日期:2015-10-01 00:00:00

  • Regulatory exaptation of the catabolite repression protein (Crp)-cAMP system in Pseudomonas putida.

    abstract::The genome of the soil bacterium Pseudomonas putida KT2440 encodes singular orthologues of genes crp (encoding the catabolite repression protein, Crp) and cyaA (adenylate cyclase) of Escherichia coli. The levels of cAMP formed by P. putida cells were below detection with a Dictyostelium biosensor in vivo. The cyaA(P. ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02331.x

    authors: Milanesio P,Arce-Rodríguez A,Muñoz A,Calles B,de Lorenzo V

    更新日期:2011-02-01 00:00:00