Pyomelanin-producing Pseudomonas aeruginosa selected during chronic infections have a large chromosomal deletion which confers resistance to pyocins.

Abstract:

:When bacterial lineages make the transition from free-living to permanent association with hosts, they can undergo massive gene losses, for which the selective forces within host tissues are unknown. We identified here melanogenic clinical isolates of Pseudomonas aeruginosa with large chromosomal deletions (66 to 270 kbp) and characterized them to investigate how they were selected. When compared with their wild-type parents, melanogenic mutants (i) exhibited a lower fitness in growth conditions found in human tissues, such as hyperosmolarity and presence of aminoglycoside antibiotics, (ii) narrowed their metabolic spectrum with a growth disadvantage with particular carbon sources, including aromatic amino acids and acyclic terpenes, suggesting a reduction of metabolic flexibility. Despite an impaired fitness in rich media, melanogenic mutants can inhibit their wild-type parents and compete with them in coculture. Surprisingly, melanogenic mutants became highly resistant to two intraspecific toxins, the S-pyocins AP41 and S1. Our results suggest that pyocins produced within a population of infecting P. aeruginosa may have selected for bacterial mutants that underwent massive gene losses and that were adapted to the life in diverse bacterial communities in the human host. Intraspecific interactions may therefore be an important factor driving the continuing evolution of pathogens during host infections.

journal_name

Environ Microbiol

authors

Hocquet D,Petitjean M,Rohmer L,Valot B,Kulasekara HD,Bedel E,Bertrand X,Plésiat P,Köhler T,Pantel A,Jacobs MA,Hoffman LR,Miller SI

doi

10.1111/1462-2920.13336

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

3482-3493

issue

10

eissn

1462-2912

issn

1462-2920

journal_volume

18

pub_type

杂志文章
  • Microbial succession of nitrate-reducing bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps.

    abstract::Changes in community structure and activity of the dissimilatory nitrate-reducing community were investigated across a glacier foreland in the Central Alps to gain insight into the successional pattern of this functional group and the driving environmental factors. Bulk soil and rhizosphere soil of Poa alpina was samp...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01051.x

    authors: Deiglmayr K,Philippot L,Tscherko D,Kandeler E

    更新日期:2006-09-01 00:00:00

  • Non-native acylated homoserine lactones reveal that LuxIR quorum sensing promotes symbiont stability.

    abstract::Quorum sensing, a group behaviour coordinated by a diffusible pheromone signal and a cognate receptor, is typical of bacteria that form symbioses with plants and animals. LuxIR-type N-acyl L-homoserine (AHL) quorum sensing is common in Gram-negative Proteobacteria, and many members of this group have additional quorum...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12322

    authors: Studer SV,Schwartzman JA,Ho JS,Geske GD,Blackwell HE,Ruby EG

    更新日期:2014-08-01 00:00:00

  • Persister cells resuscitate via ribosome modification by 23S rRNA pseudouridine synthase RluD.

    abstract::Upon a wide range of stress conditions (e.g. nutrient, antibiotic, oxidative), a subpopulation of bacterial cells known as persisters survives by halting metabolism. These cells resuscitate rapidly to reconstitute infections once the stress is removed and nutrients are provided. However, how these dormant cells resusc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14828

    authors: Song S,Wood TK

    更新日期:2020-03-01 00:00:00

  • Ectomycorrhizal fungi inoculation alleviates simulated acid rain effects on soil ammonia oxidizers and denitrifiers in Masson pine forest.

    abstract::Acid rain can cause severe effects on soil biota and nutrient biogeochemical cycles in the forest ecosystem, but how plant-symbiotic ectomycorrhizal fungi will modulate the effects remains unknown. Here, we conducted a full factorial field experiment in a Masson pine forest by simultaneously controlling the acidity of...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14457

    authors: Li Y,Chen Z,He JZ,Wang Q,Shen C,Ge Y

    更新日期:2019-01-01 00:00:00

  • Involvement of the ABC transporter BcAtrB and the laccase BcLCC2 in defence of Botrytis cinerea against the broad-spectrum antibiotic 2,4-diacetylphloroglucinol.

    abstract::The genetic and biochemical basis of defence mechanisms in plant pathogenic fungi against antifungal compounds produced by antagonistic microorganisms is largely unknown. The results of this study show that both degradative and non-degradative defence mechanisms enable the plant pathogenic fungus Botrytis cinerea to r...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01531.x

    authors: Schouten A,Maksimova O,Cuesta-Arenas Y,van den Berg G,Raaijmakers JM

    更新日期:2008-05-01 00:00:00

  • Interclonal gradient of virulence in the Pseudomonas aeruginosa pangenome from disease and environment.

    abstract::The population genomics of Pseudomonas aeruginosa was analysed by genome sequencing of representative strains of the 15 most frequent clonal complexes in the P. aeruginosa population and of the five most common clones from the environment of which so far no isolate from a human infection has been detected. Gene annota...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12606

    authors: Hilker R,Munder A,Klockgether J,Losada PM,Chouvarine P,Cramer N,Davenport CF,Dethlefsen S,Fischer S,Peng H,Schönfelder T,Türk O,Wiehlmann L,Wölbeling F,Gulbins E,Goesmann A,Tümmler B

    更新日期:2015-01-01 00:00:00

  • A wide diversity of previously undetected free-living relatives of diplomonads isolated from marine/saline habitats.

    abstract::Over the last 15 years classical culturing and environmental PCR techniques have revealed a modest number of genuinely new major lineages of protists; however, some new groups have greatly influenced our understanding of eukaryote evolution. We used culturing techniques to examine the diversity of free-living protists...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02239.x

    authors: Kolisko M,Silberman JD,Cepicka I,Yubuki N,Takishita K,Yabuki A,Leander BS,Inouye I,Inagaki Y,Roger AJ,Simpson AG

    更新日期:2010-10-01 00:00:00

  • Effect of banning vancomycin analogue avoparcin on vancomycin-resistant enterococci in chicken farms in Taiwan.

    abstract::Avoparcin, a vancomycin analogue, was banned as a feed additive in Taiwan in 2000. A nationwide surveillance was conducted to study the prevalence of vancomycin-resistant enterococci (VRE) on chicken farms between 2000 and 2003. Among the 1021 E. faecalis and 967 E. faecium isolates studied, resistance to tetracycline...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01189.x

    authors: Lauderdale TL,Shiau YR,Wang HY,Lai JF,Huang IW,Chen PC,Chen HY,Lai SS,Liu YF,Ho M

    更新日期:2007-03-01 00:00:00

  • A novel hybrid kinase is essential for regulating the sigma(B)-mediated stress response of Bacillus cereus.

    abstract::A common bacterial strategy for monitoring environmental challenges is to use two-component systems, which consist of a sensor histidine kinase (HK) and a response regulator (RR). In the food-borne pathogen Bacillus cereus, the alternative sigma factor sigma(B) is activated by the RR RsbY. Here we present strong indic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02116.x

    authors: de Been M,Tempelaars MH,van Schaik W,Moezelaar R,Siezen RJ,Abee T

    更新日期:2010-03-01 00:00:00

  • Chemotaxis of Ralstonia sp. SJ98 towards p-nitrophenol in soil.

    abstract::Bioremediation of contaminated sites has been accepted as an efficient and cheaper alternative to physicochemical means of remediation in several cases. Although chemotactic behaviour of many bacteria has been studied earlier and assays have been developed to study bacterial chemotaxis in semi-solid media, this phenom...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01064.x

    authors: Paul D,Singh R,Jain RK

    更新日期:2006-10-01 00:00:00

  • Evolutionary innovation: a bone-eating marine symbiosis.

    abstract::Symbiotic associations between microbes and invertebrates have resulted in some of the most unusual physiological and morphological adaptations that have evolved in the animal world. We document a new symbiosis between marine polychaetes of the genus Osedax and members of the bacterial group Oceanospirillales, known f...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00824.x

    authors: Goffredi SK,Orphan VJ,Rouse GW,Jahnke L,Embaye T,Turk K,Lee R,Vrijenhoek RC

    更新日期:2005-09-01 00:00:00

  • Differential thermal adaptation of clonal strains of a protist morphospecies originating from different climatic zones.

    abstract::Eco-physiological variation and local adaptation are key issues in microbial ecology. Here, we investigated the thermal adaptation of 19 strains of the same Spumella morphospecies (Chrysophyceae, Heterokonta). In order to test for local adaptation and the existence of specific ecotypes we analysed growth rates of thes...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01175.x

    authors: Boenigk J,Jost S,Stoeck T,Garstecki T

    更新日期:2007-03-01 00:00:00

  • Bacterial diversity in hydrothermal sediment and epsilonproteobacterial dominance in experimental microcolonizers at the Mid-Atlantic Ridge.

    abstract::We report here a molecular survey based on 16S rRNA genes of the bacterial diversity found in two deep-sea vent niches at the Mid-Atlantic Ridge: hydrothermal sediment (Rainbow site), and microcolonizers made of three different substrates (organic-rich, iron-rich and pumice) that were exposed for 15 days to a vent emi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2003.00495.x

    authors: López-García P,Duperron S,Philippot P,Foriel J,Susini J,Moreira D

    更新日期:2003-10-01 00:00:00

  • Differential responses of marine, mesohaline and oligohaline bacterial communities to the addition of terrigenous carbon.

    abstract::In response to global warming, increasing quantities of tDOM are transported through estuaries from land to the sea. In this study, we investigated microbial responses to increased tDOM concentrations in three salinity regimes (salinity: 32, 7 and 3) characteristic of the Baltic Sea. Mesocosm experiments performed in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13784

    authors: Herlemann DPR,Manecki M,Dittmar T,Jürgens K

    更新日期:2017-08-01 00:00:00

  • Cyanobacterial assimilatory nitrate reductase gene diversity in coastal and oligotrophic marine environments.

    abstract::Cyanobacteria are important primary producers in many marine ecosystems and their abundances and growth rates depend on their ability to assimilate various nitrogen sources. To examine the diversity of nitrate-utilizing marine cyanobacteria, we developed PCR primers specific for cyanobacterial assimilatory nitrate red...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01084.x

    authors: Jenkins BD,Zehr JP,Gibson A,Campbell L

    更新日期:2006-12-01 00:00:00

  • The faulty SOS response of Pseudomonas putida KT2440 stems from an inefficient RecA-LexA interplay.

    abstract::Despite its environmental robustness Pseudomonas putida strain KT2440 is very sensitive to DNA damage and displays poor homologous recombination efficiencies. To gain an insight into this deficiency isogenic ∆recA and ∆lexA1 derivatives of prophage-free strain P. putida EM173 were generated and responses of the recA a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15384

    authors: Akkaya Ö,Aparicio T,Pérez-Pantoja D,de Lorenzo V

    更新日期:2021-01-03 00:00:00

  • Micromanipulation and further identification of FISH-labelled microcolonies of a dominant denitrifying bacterium in activated sludge.

    abstract::The activated sludge process relies on the formation of strong microbial flocs. The knowledge about dominant floc-forming bacteria is at present very limited, especially from a phylogenetic perspective. In this study, numerous microcolonies in the activated sludge flocs were found to be targeted by a Betaproteobacteri...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00580.x

    authors: Thomsen TR,Nielsen JL,Ramsing NB,Nielsen PH

    更新日期:2004-05-01 00:00:00

  • Syntrophic associations from hypersaline soda lakes converting organic acids and alcohols to methane at extremely haloalkaline conditions.

    abstract::Until now anaerobic oxidation of VFA at high salt-pH has been demonstrated only at sulfate-reducing conditions. Here, we present results of a microbiological investigation of anaerobic conversion of organic acids and alcohols at methanogenic conditions by syntrophic associations enriched from hypersaline soda lakes in...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13448

    authors: Sorokin DY,Abbas B,Geleijnse M,Kolganova TV,Kleerebezem R,van Loosdrecht MC

    更新日期:2016-09-01 00:00:00

  • Organohalide respiration in pristine environments: implications for the natural halogen cycle.

    abstract::Halogenated organic compounds, also termed organohalogens, were initially considered to be of almost exclusively anthropogenic origin. However, over 5000 naturally synthesized organohalogens are known today. This has also fuelled the hypothesis that the natural and ancient origin of organohalogens could have primed de...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.14016

    authors: Atashgahi S,Häggblom MM,Smidt H

    更新日期:2018-03-01 00:00:00

  • Analysis of the microbial community structure and function of a laboratory scale enhanced biological phosphorus removal reactor.

    abstract::A laboratory scale sequencing batch reactor (SBR) operating for enhanced biological phosphorus removal (EBPR) and fed with a mixture of volatile fatty acids (VFAs) showed stable and efficient EBPR capacity over a four-year-period. Phosphorus (P), poly-beta-hydroxyalkanoate (PHA) and glycogen cycling consistent with cl...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00339.x

    authors: Levantesi C,Serafim LS,Crocetti GR,Lemos PC,Rossetti S,Blackall LL,Reis MA,Tandoi V

    更新日期:2002-10-01 00:00:00

  • Identification and analysis of the bacterial endosymbiont specialized for production of the chemotherapeutic natural product ET-743.

    abstract::Ecteinascidin 743 (ET-743, Yondelis) is a clinically approved chemotherapeutic natural product isolated from the Caribbean mangrove tunicate Ecteinascidia turbinata. Researchers have long suspected that a microorganism may be the true producer of the anticancer drug, but its genome has remained elusive due to our inab...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12908

    authors: Schofield MM,Jain S,Porat D,Dick GJ,Sherman DH

    更新日期:2015-10-01 00:00:00

  • Identification of a microorganism that links its growth to the reductive dechlorination of 2,3,5,6-chlorobiphenyl.

    abstract::Anaerobic bacteria reductively dechlorinate polychlorinated biphenyls (PCBs) in aquatic sediments, but these microorganisms remain uncultured and, until now, unidentified. Through denaturing gradient gel electrophoresis (DGGE) of 16S rDNA from a highly enriched ortho-PCB dechlorinating culture, the growth of a single ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00246.x

    authors: Cutter LA,Watts JE,Sowers KR,May HD

    更新日期:2001-11-01 00:00:00

  • Catalases play differentiated roles in the adaptation of a fungal entomopathogen to environmental stresses.

    abstract::The catalase family of Beauveria bassiana (fungal entomopathogen) consists of catA (spore-specific), catB (secreted), catP (peroxisomal), catC (cytoplasmic) and catD (secreted peroxidase/catalase), which were distinguished in phylogeny and structure and functionally characterized by constructing single-gene disrupted ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02848.x

    authors: Wang ZL,Zhang LB,Ying SH,Feng MG

    更新日期:2013-02-01 00:00:00

  • Transcription of a 'photosynthetic' T4-type phage during infection of a marine cyanobacterium.

    abstract::The transcription of S-PM2 phage following infection of Synechococcus sp. WH7803, a marine cyanobacterium, was analysed by quantitative real-time PCR. Unlike the distantly related coliphage T4, there were only two (early and late) instead of three (early, middle and late) classes of transcripts during the developmenta...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00969.x

    authors: Clokie MR,Shan J,Bailey S,Jia Y,Krisch HM,West S,Mann NH

    更新日期:2006-05-01 00:00:00

  • Worldwide distribution of Pseudomonas aeruginosa clone C strains in the aquatic environment and cystic fibrosis patients.

    abstract::Highly successful bacterial clones have the ability to effectively colonize environmental niches and patients. However, the factors which determine the complex interplay between the colonization of environmental niches and patients are mainly unknown. In this study we show that Pseudomonas aeruginosa clone C strains a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00780.x

    authors: Römling U,Kader A,Sriramulu DD,Simm R,Kronvall G

    更新日期:2005-07-01 00:00:00

  • Control and host-dependent activation of insect toxin expression in a root-associated biocontrol pseudomonad.

    abstract::Pseudomonas fluorescens CHA0 is a root-associated biocontrol agent that suppresses soil-borne fungal diseases of crops. Remarkably, the pseudomonad is also endowed with systemic and oral activity against pest insects which depends on the production of the insecticidal Fit toxin. The toxin gene (fitD) is part of a viru...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12050

    authors: Péchy-Tarr M,Borel N,Kupferschmied P,Turner V,Binggeli O,Radovanovic D,Maurhofer M,Keel C

    更新日期:2013-03-01 00:00:00

  • Metatranscriptomic array analysis of 'Candidatus Accumulibacter phosphatis'-enriched enhanced biological phosphorus removal sludge.

    abstract::Here we report the first metatranscriptomic analysis of gene expression and regulation of 'Candidatus Accumulibacter'-enriched lab-scale sludge during enhanced biological phosphorus removal (EBPR). Medium density oligonucleotide microarrays were generated with probes targeting most predicted genes hypothesized to be i...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02163.x

    authors: He S,Kunin V,Haynes M,Martin HG,Ivanova N,Rohwer F,Hugenholtz P,McMahon KD

    更新日期:2010-05-01 00:00:00

  • Relative contributions of archaea and bacteria to aerobic ammonia oxidation in the environment.

    abstract::Traditionally, organisms responsible for major biogeochemical cycling processes have been determined by physiological characterization of environmental isolates in laboratory culture. Molecular techniques have, however, confirmed the widespread occurrence of abundant bacterial and archaeal groups with no cultivated re...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1462-2920.2008.01775.x

    authors: Prosser JI,Nicol GW

    更新日期:2008-11-01 00:00:00

  • Expression and regulation of the mer operon in Thermus thermophilus.

    abstract::Mercury (Hg) is a highly toxic and widely distributed heavy metal, which some Bacteria and Archaea detoxify by the reduction of ionic Hg (Hg[II]) to the elemental volatile form, Hg(0). This activity is specified by the mer operon. The mer operon of the deeply branching thermophile Thermus thermophilus HB27 encodes for...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14953

    authors: Norambuena J,Miller M,Boyd JM,Barkay T

    更新日期:2020-04-01 00:00:00

  • Changes in the community structure and activity of betaproteobacterial ammonia-oxidizing sediment bacteria along a freshwater-marine gradient.

    abstract::To determine whether the distribution of estuarine ammonia-oxidizing bacteria (AOB) was influenced by salinity, the community structure of betaproteobacterial ammonia oxidizers (AOB) was characterized along a salinity gradient in sediments of the Ythan estuary, on the east coast of Scotland, UK, by denaturant gradient...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00947.x

    authors: Freitag TE,Chang L,Prosser JI

    更新日期:2006-04-01 00:00:00