Abstract:
:When bacterial lineages make the transition from free-living to permanent association with hosts, they can undergo massive gene losses, for which the selective forces within host tissues are unknown. We identified here melanogenic clinical isolates of Pseudomonas aeruginosa with large chromosomal deletions (66 to 270 kbp) and characterized them to investigate how they were selected. When compared with their wild-type parents, melanogenic mutants (i) exhibited a lower fitness in growth conditions found in human tissues, such as hyperosmolarity and presence of aminoglycoside antibiotics, (ii) narrowed their metabolic spectrum with a growth disadvantage with particular carbon sources, including aromatic amino acids and acyclic terpenes, suggesting a reduction of metabolic flexibility. Despite an impaired fitness in rich media, melanogenic mutants can inhibit their wild-type parents and compete with them in coculture. Surprisingly, melanogenic mutants became highly resistant to two intraspecific toxins, the S-pyocins AP41 and S1. Our results suggest that pyocins produced within a population of infecting P. aeruginosa may have selected for bacterial mutants that underwent massive gene losses and that were adapted to the life in diverse bacterial communities in the human host. Intraspecific interactions may therefore be an important factor driving the continuing evolution of pathogens during host infections.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Hocquet D,Petitjean M,Rohmer L,Valot B,Kulasekara HD,Bedel E,Bertrand X,Plésiat P,Köhler T,Pantel A,Jacobs MA,Hoffman LR,Miller SIdoi
10.1111/1462-2920.13336subject
Has Abstractpub_date
2016-10-01 00:00:00pages
3482-3493issue
10eissn
1462-2912issn
1462-2920journal_volume
18pub_type
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