Abstract:
:Bioremediation of contaminated sites has been accepted as an efficient and cheaper alternative to physicochemical means of remediation in several cases. Although chemotactic behaviour of many bacteria has been studied earlier and assays have been developed to study bacterial chemotaxis in semi-solid media, this phenomenon has never been demonstrated in soil. For bioremediation application it is important to know whether bacteria actually migrate through the heterogenous soil medium towards a gradient of a particular chemoattractant. In the present study we have successfully demonstrated bacterial chemotaxis of a Ralstonia sp. SJ98 in soil microcosm using qualitative and quantitative plate and tray assays. The migration of bacteria has been established using several methods such as plate counting, vital staining and flow cytometry and slot blot hybridization. A non-chemotactic p-nitrophenol utilizing strain Burkholderia cepacia RKJ200 has been used as negative control. Our work clearly substantiates the hypothesis that chemotactic bacteria may enhance in situ bioremediation of toxic pollutants from soils and sediments.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Paul D,Singh R,Jain RKdoi
10.1111/j.1462-2920.2006.01064.xsubject
Has Abstractpub_date
2006-10-01 00:00:00pages
1797-804issue
10eissn
1462-2912issn
1462-2920pii
EMI1064journal_volume
8pub_type
杂志文章abstract::Iron is the most abundant redox-active metal in the Earth's crust. The one electron transfer between the two most common redox states, Fe(II) and Fe(III), plays a role in a huge range of environmental processes from mineral formation and dissolution to contaminant remediation and global biogeochemical cycling. It has ...
journal_title:Environmental microbiology
pub_type: 杂志文章,评审
doi:10.1111/1462-2920.14328
更新日期:2018-10-01 00:00:00
abstract::Phosphonoacetate is regarded as an antiviral xenobiotic whose mineralization can be catalysed by an enzyme, phosphonoacetate hydrolase, encoded by the phnA gene. To date the enzyme's activity has been detected in only a limited number of bacteria. Its expression has been shown to occur in a manner independent of the p...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2005.00974.x
更新日期:2006-05-01 00:00:00
abstract::Enhanced biological phosphorus removal (EBPR) exploits the metabolism of polyphosphate-accumulating organisms (PAOs) to remove excess phosphorus (P) from wastewater treatment. Candidatus Accumulibacter phosphatis (Accumulibacter) is the most abundant and well-studied PAO in EBPR systems. In a previous study, we detect...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14576
更新日期:2019-04-01 00:00:00
abstract::The rice blast fungus Magnaporthe oryzae has eight regulators of G-protein signaling (RGS) and RGS-like proteins (MoRgs1 to MoRgs8) that exhibit both distinct and shared regulatory functions in the growth, differentiation and pathogenicity of the fungus. We found MoRgs7 with a unique RGS-seven transmembrane (7-TM) dom...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14102
更新日期:2018-09-01 00:00:00
abstract::An organism's metabolic activity leaves an extracellular footprint and dynamic changes in this exometabolome inform about nutrient uptake, waste disposal and signalling activities. Using non-targeted mass spectrometry, we report exometabolome dynamics of hypoxia-induced, non-replicating mycobacteria that are thought t...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13056
更新日期:2015-11-01 00:00:00
abstract::Many plant-associated fungi host endosymbiotic endobacteria with reduced genomes. While endobacteria play important roles in these tri-partite plant-fungal-endobacterial systems, the active physiology of fungal endobacteria has not been characterized extensively by systems biology approaches. Here, we use integrated p...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13605
更新日期:2017-03-01 00:00:00
abstract::The bacterial metabolite and transition metal chelator pyridine-2,6-dithiocarboxylic acid (PDTC), promotes a novel and effective means of dechlorination of the toxic and carcinogenic pollutant, carbon tetrachloride. Pyridine-2,6-dithiocarboxylic acid has been presumed to act as a siderophore in the Pseudomonas strains...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1046/j.1462-2920.2003.00558.x
更新日期:2004-02-01 00:00:00
abstract::Sinorhizobium fredii HH103 RifR , a broad-host-range rhizobial strain, forms ineffective nodules with Lotus japonicus but induces nitrogen-fixing nodules in Lotus burttii roots that are infected by intercellular entry. Here we show that HH103 RifR nolR or nodD2 mutants gain the ability to induce infection thread forma...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14584
更新日期:2019-05-01 00:00:00
abstract::Intensive microbial growth typically observed in laboratory rarely occurs in nature. Because of severe nutrient deficiency, natural populations exhibit near-zero growth (NZG). There is a long-standing controversy about sustained NZG, specifically whether there is a minimum growth rate below which cells die or whether ...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12584
更新日期:2015-01-01 00:00:00
abstract::Stable isotope labelling of amino acids in cell culture (SILAC) is a quantitative proteomic method that can illuminate new pathways used by cells to adapt to different lifestyles and niches. Archaea, while thriving in extreme environments and accounting for ∼20%-40% of the Earth's biomass, have not been analyzed with ...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14014
更新日期:2018-01-01 00:00:00
abstract::In diseased piglets from two Dutch pig-breeding farms with neonatal diarrhoea for more than a year, culture and PCR analyses identified the involved microorganism as Clostridium difficile PCR ribotype 078 harbouring toxin A (tcdA) and B (tcdB), and binary toxin genes. Isolated strains showed a 39 bp deletion in the tc...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2008.01790.x
更新日期:2009-02-01 00:00:00
abstract::Despite recognition of the importance of soil bacteria to terrestrial ecosystem functioning there is little consensus on the factors regulating belowground biodiversity. Here we present a multi-scale spatial assessment of soil bacterial community profiles across Great Britain (> 1000 soil cores), and show the first la...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2011.02480.x
更新日期:2011-06-01 00:00:00
abstract::Growing evidence indicates that dense water formation and flow over the continental shelf is a globally relevant oceanographic process, potentially affecting microbial assemblages down to the deep ocean. However, the extent and consequences of this influence have yet to be investigated. Here it is shown that dense wat...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13510
更新日期:2016-12-01 00:00:00
abstract::Biofilms are dynamic players in biogeochemical cycling in running waters and are subjected to environmental stressors like those provoked by climate change. We investigated whether a 2°C increase in flowing water would affect prokaryotic community composition and heterotrophic metabolic activities of biofilms grown un...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12428
更新日期:2014-08-01 00:00:00
abstract::In this study, the ability of the geoactive fungus Aspergillus niger to colonize and transform manganese nodules from the Clarion-Clipperton Zone in both solid and liquid media was investigated. Aspergillus niger was able to colonize and penetrate manganese nodules embedded in solid medium and effect extensive transfo...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14591
更新日期:2019-05-01 00:00:00
abstract::The wide distribution of diverse nitrogenase (nifH) genes affiliated with those of heterotrophic bacteria in marine and estuarine waters indicates ubiquity and an ecologically relevant role for heterotrophic N2 -fixers (diazotrophs) in aquatic nitrogen (N) cycling. However, the lack of cultivated representatives curre...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12335
更新日期:2014-10-01 00:00:00
abstract::Fruiting body development and carotenoid production are light-induced in Cordyceps militaris. Our previous studies have shown that two blue-light receptors, CmWC-1 and CmCRY-DASH, regulate fruiting body development and secondary metabolism. However, the photosensory system of C. militaris remains unclear. Here, gene d...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.14867
更新日期:2020-01-01 00:00:00
abstract::In order to elucidate the main predictors of Vibrio cholerae dynamics and to estimate the risk of Vibrio cholera-related diseases, a recently developed direct detection approach based on fluorescence in situ hybridization and solid-phase cytometry (CARD-FISH/SPC) was applied in comparison to cultivation for water samp...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12861
更新日期:2015-11-01 00:00:00
abstract::The high genomic G+C group of Actinobacteria possesses a variety of physiological and metabolic properties, and exhibits diverse lifestyles and ecological distribution. In recent years, Actinobacteria have been found to frequently dominate samples obtained from freshwater samples. Furthermore, phylogenetic analyses ha...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2009.02024.x
更新日期:2009-12-01 00:00:00
abstract::Cell cycle regulation is pivotal for proper cell division and cellular differentiation in eukaryotic cells. The central regulators that govern eukaryotic cell cycle progression are cyclin-dependent kinases (CDKs) and their partners. Here, we report that Magnaporthe oryzae CKS1 encodes a cyclin-dependent kinase subunit...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13796
更新日期:2017-10-01 00:00:00
abstract::An enriched sulphidogenic consortium capable of mineralizing benzene was used to study the metabolic pathway of anaerobic benzene degradation. Benzoate was detected in active cultures and benzene was confirmed to be the source of this benzoate by the addition of deuterated benzene (D6) and subsequent detection of deut...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1046/j.1462-2920.2001.00228.x
更新日期:2001-09-01 00:00:00
abstract::Marine Synechococcus are frequently found in environments where iron (Fe) is a limiting nutrient. To understand their capacity to respond to Fe stress, we screened picoplankton genomes and the Global Ocean Survey metagenome for known Fe stress genes. Many open ocean strains of Synechococcus lack most known genes for F...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2008.01778.x
更新日期:2009-02-01 00:00:00
abstract::Tropical ecosystems remain poorly understood and this is particularly true for belowground soil fungi. Soil fungi may respond to plant identity when, for example, plants differentially allocate resources belowground. However, spatial and temporal heterogeneity in factors such as plant inputs, moisture, or nutrients ca...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13342
更新日期:2016-12-01 00:00:00
abstract::Here we describe the application of a new click chemistry method for fluorescent tracking of protein synthesis in individual microorganisms within environmental samples. This technique, termed bioorthogonal non-canonical amino acid tagging (BONCAT), is based on the in vivo incorporation of the non-canonical amino acid...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.12436
更新日期:2014-08-01 00:00:00
abstract::When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2008.01658.x
更新日期:2008-09-01 00:00:00
abstract::This study presents comparative geochemical, microbiological and molecular analyses of Greenland GISP2 ice core samples representing different depths, ages, deposition climates, in situ temperatures, and gas and ionic compositions. Our goal was to determine whether specific organisms, preserved at different depths, co...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2008.01835.x
更新日期:2009-03-01 00:00:00
abstract::In vitro studies in plant, soil, and human systems have shown that microbial volatiles can mediate microbe-microbe or microbe-host interactions. These previous studies have often used artificially high concentrations of volatiles compared to in situ systems and have not demonstrated the roles volatiles play in mediati...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.15223
更新日期:2020-11-01 00:00:00
abstract::Endophytic microorganisms asymptomatically colonise plant tissues. Exploring the assembly dynamics of bacterial endophytic communities is essential to understand the functioning of the plant holobiont and to optimise their possible use as biopesticides or plant biostimulants. The variation in endophytic communities in...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/1462-2920.13843
更新日期:2017-08-01 00:00:00
abstract::Two cellulose-fermenting methanogenic enrichment cultures originating from rice soil, one at 15 degrees C with Methanosaeta and the other at 30 degrees C with Methanosarcina as the dominant acetoclastic methanogen, both degraded cellulose anaerobically via propionate, acetate and H2 to CH4. The degradation was a two-s...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1046/j.1462-2920.2001.00199.x
更新日期:2001-06-01 00:00:00
abstract::Molecular signatures of new, as yet uncultured mollicute-like organisms (MLOs) have been detected in total rRNA and DNA extracted from tissues, gut contents and casts of four species of the earthworm family Lumbricidae. The MLO 16S rRNA sequences exhibited low identity to those of known Mollicutes species and formed a...
journal_title:Environmental microbiology
pub_type: 杂志文章
doi:10.1111/j.1462-2920.2008.01837.x
更新日期:2009-04-01 00:00:00