Abstract:
:An enriched sulphidogenic consortium capable of mineralizing benzene was used to study the metabolic pathway of anaerobic benzene degradation. Benzoate was detected in active cultures and benzene was confirmed to be the source of this benzoate by the addition of deuterated benzene (D6) and subsequent detection of deuterated benzoate (D5) in active cultures but not in autoclaved controls. Benzoate was utilized by this culture at 1/12 the rate of benzene, while its presence did not inhibit benzene utilization. The benzene utilization rate was reduced, however, in the presence of 2-fluorobenzoate. When the culture was supplemented with [(13)C]-bicarbonate, the carboxyl group on benzoate was not labelled with [(13)C]-carbon, suggesting that this transformation relies on a more complex set of reactions than simple addition of carbonate.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Phelps CD,Zhang X,Young LYdoi
10.1046/j.1462-2920.2001.00228.xkeywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
600-3issue
9eissn
1462-2912issn
1462-2920pii
228journal_volume
3pub_type
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