Abstract:
:Many plant-associated fungi host endosymbiotic endobacteria with reduced genomes. While endobacteria play important roles in these tri-partite plant-fungal-endobacterial systems, the active physiology of fungal endobacteria has not been characterized extensively by systems biology approaches. Here, we use integrated proteomics and metabolomics to characterize the relationship between the endobacterium Mycoavidus sp. and the root-associated fungus Mortierella elongata. In nitrogen-poor media, M. elongata had decreased growth but hosted a large and growing endobacterial population. The active endobacterium likely extracted malate from the fungal host as the primary carbon substrate for energy production and biosynthesis of phospho-sugars, nucleobases, peptidoglycan and some amino acids. The endobacterium obtained nitrogen by importing a variety of nitrogen-containing compounds. Further, nitrogen limitation significantly perturbed the carbon and nitrogen flows in the fungal metabolic network. M. elongata regulated many pathways by concordant changes on enzyme abundances, post-translational modifications, reactant concentrations and allosteric effectors. Such multimodal regulations may be a general mechanism for metabolic modulation.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Li Z,Yao Q,Dearth SP,Entler MR,Castro Gonzalez HF,Uehling JK,Vilgalys RJ,Hurst GB,Campagna SR,Labbé JL,Pan Cdoi
10.1111/1462-2920.13605subject
Has Abstractpub_date
2017-03-01 00:00:00pages
1041-1053issue
3eissn
1462-2912issn
1462-2920journal_volume
19pub_type
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