Exposure to soil, house dust and decaying plants increases gut microbial diversity and decreases serum immunoglobulin E levels in BALB/c mice.

Abstract:

:To assess the impact of sanitation of a living environment on gut microbiota and development of the immune system, we raised BALB/c mice under three distinct environmental conditions: a specific pathogen-free animal room (SPF), a general animal room (XZ) and a farmhouse (JD). All other variables like diet, age, genetic background, physiological status and original gut microbiota were controlled for in the three groups. Using high-throughput sequencing of the 16S rRNA gene, we found that each mouse group had a specific structure of the gut microbial community. Groups JD and XZ harboured a significantly more diverse and richer gut microbiota than did group SPF. Bacteroidetes were significantly more abundant in groups XZ and JD than in group SPF, whereas Firmicutes showed the inverse pattern. Total serum immunoglobulin E (IgE) levels were significantly lower in groups XZ and JD than in group SPF. There were no significant differences in gut microbiota diversity and serum IgE concentration between groups JD and XZ, but we found higher abundance of dominant genera in the gut microflora of group JD. We conclude that exposure to soil, house dust and decaying plant material enhances gut microbial diversity and innate immunity. Our results seem to provide new evidence supporting the hygiene hypothesis.

journal_name

Environ Microbiol

authors

Zhou D,Zhang H,Bai Z,Zhang A,Bai F,Luo X,Hou Y,Ding X,Sun B,Sun X,Ma N,Wang C,Dai X,Lu Z

doi

10.1111/1462-2920.12895

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

1326-37

issue

5

eissn

1462-2912

issn

1462-2920

journal_volume

18

pub_type

杂志文章
  • Roseobacter and SAR11 dominate microbial glucose uptake in coastal North Sea waters.

    abstract::Bacterial assemblages in coastal pelagic environments are exposed to pronounced temporal and spatial fluctuations in the availability of monomeric substrates. Little is known about the response of particular bacterial groups to such variability. We studied glucose incorporation at various concentrations (0.1-100 nM) b...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01082.x

    authors: Alonso C,Pernthaler J

    更新日期:2006-11-01 00:00:00

  • Chromomycin, an antibiotic produced by Streptomyces flaviscleroticus might play a role in the resistance to oxidative stress and is essential for viability in stationary phase.

    abstract::The well-known role of antibiotics in killing sensitive organisms has been challenged by the effects they exert at subinhibitory concentrations. Unfortunately, there are very few published reports on the advantages these molecules may confer to their producers. This study describes the construction of a genetically ve...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14515

    authors: Prajapati D,Kumari N,Dave K,Chatupale V,Pohnerkar J

    更新日期:2019-02-01 00:00:00

  • Molecular characterization of thermostable direct haemolysin-related haemolysin (TRH)-positive Vibrio parahaemolyticus from oysters in Mangalore, India.

    abstract::Pathogenic Vibrio parahaemolyticus strains producing either or both of a thermostable direct haemolysin (TDH) and a TDH-related haemolysin (TRH) encoded by tdh and trh genes, respectively, are isolated at a low rate from the environment. However, recently we observed that a considerable percentage of APW (alkaline pep...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.00990.x

    authors: Parvathi A,Kumar HS,Bhanumathi A,Ishibashi M,Nishibuchi M,Karunasagar I,Karunasagar I

    更新日期:2006-06-01 00:00:00

  • Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment.

    abstract::Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies-carbon-flow during anaerobic OM degradation in arctic marine ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14297

    authors: Müller AL,Pelikan C,de Rezende JR,Wasmund K,Putz M,Glombitza C,Kjeldsen KU,Jørgensen BB,Loy A

    更新日期:2018-08-01 00:00:00

  • Attachment between heterotrophic bacteria and microalgae influences symbiotic microscale interactions.

    abstract::The surface and surroundings of microalgal cells (phycosphere) are critical interaction zones but have been difficult to functionally interrogate due to methodological limitations. We examined effects of phycosphere-associated bacteria for two biofuel-relevant microalgal species (Phaeodactylum tricornutum and Nannochl...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14357

    authors: Samo TJ,Kimbrel JA,Nilson DJ,Pett-Ridge J,Weber PK,Mayali X

    更新日期:2018-12-01 00:00:00

  • Detection of glycolate oxidase gene glcD diversity among cultured and environmental marine bacteria.

    abstract::Of eight laboratory cultures of marine gamma- and alpha-Proteobacteria tested, growth on glycolate as a sole carbon source was detected for only three species: Pseudomonas stutzeri, Oceanimonas doudoroffii and Roseobacter sp. isolate Y3F. Degenerate polymerase chain reaction (PCR) primers were designed to amplify glcD...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01092.x

    authors: Lau WW,Armbrust EV

    更新日期:2006-10-01 00:00:00

  • Coordinate overexpression of two RND efflux systems, ParXY and TtgABC, is responsible for multidrug resistance in Pseudomonas putida.

    abstract::Resistance Nodulation cell Division (RND) efflux pumps are known to contribute to the tolerance of Pseudomonas putida to aromatic hydrocarbons, but their role in antibiotic resistance has not been fully elucidated. In this study, two types of single-step multidrug-resistant (MDR) mutants were selected in vitro from re...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15200

    authors: Puja H,Comment G,Chassagne S,Plésiat P,Jeannot K

    更新日期:2020-12-01 00:00:00

  • Methyl/alkyl-coenzyme M reductase-based anaerobic alkane oxidation in archaea.

    abstract::Methyl-coenzyme M reductase (MCR) has been originally identified to catalyse the final step of the methanogenesis pathway. About 20 years ago anaerobic methane-oxidizing archaea (ANME) were discovered that use MCR enzymes to activate methane. ANME thrive at the thermodynamic limit of life, are slow-growing, and in mos...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.15057

    authors: Wang Y,Wegener G,Ruff SE,Wang F

    更新日期:2020-05-04 00:00:00

  • Increases in temperature and nutrient availability positively affect methane-cycling microorganisms in Arctic thermokarst lake sediments.

    abstract::Arctic permafrost soils store large amounts of organic matter that is sensitive to temperature increases and subsequent microbial degradation to methane (CH 4 ) and carbon dioxide (CO 2 ). Here, we studied methanogenic and methanotrophic activity and community composition in thermokarst lake sediments from Utqiag˙vik ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14345

    authors: de Jong AEE,In 't Zandt MH,Meisel OH,Jetten MSM,Dean JF,Rasigraf O,Welte CU

    更新日期:2018-12-01 00:00:00

  • Succession and dynamics of Pristionchus nematodes and their microbiome during decomposition of Oryctes borbonicus on La Réunion Island.

    abstract::Insects and nematodes represent the most species-rich animal taxa and they occur together in a variety of associations. Necromenic nematodes of the genus Pristionchus are found on scarab beetles with more than 30 species known from worldwide samplings. However, little is known about the dynamics and succession of nema...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13697

    authors: Meyer JM,Baskaran P,Quast C,Susoy V,Rödelsperger C,Glöckner FO,Sommer RJ

    更新日期:2017-04-01 00:00:00

  • In vitro and in vivo expression of virulence genes in Vibrio isolates belonging to the Harveyi clade in relation to their virulence towards gnotobiotic brine shrimp (Artemia franciscana).

    abstract::Vibrios belonging to the Harveyi clade are pathogenic marine bacteria affecting both vertebrates and invertebrates, thereby causing a severe threat to the aquaculture industry. In this study, the expression of haemolysin, metalloprotease, serine protease, the quorum sensing master regulator LuxR and the virulence regu...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02354.x

    authors: Ruwandeepika HA,Defoirdt T,Bhowmick PP,Karunasagar I,Karunasagar I,Bossier P

    更新日期:2011-02-01 00:00:00

  • Divinyl chlorophyll a in the marine eukaryotic protist Alexandrium ostenfeldii (Dinophyceae).

    abstract::Here it is reported the first detection of DV-chl a together with the usual chl a in the marine dinoflagellate Alexandrium ostenfeldii from the Baltic Sea. Growth response and photosynthetic parameters were examined at two irradiances (80 and 240 μmol photons m(-2) s(-1)) and temperatures (15 °C and 19 °C) in a diviny...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13042

    authors: Rodríguez F,Garrido JL,Sobrino C,Johnsen G,Riobó P,Franco J,Aamot I,Ramilo I,Sanz N,Kremp A

    更新日期:2016-02-01 00:00:00

  • Fingerprinting the genetic diversity of the biotin carboxylase gene (accC) in aquatic ecosystems as a potential marker for studies of carbon dioxide assimilation in the dark.

    abstract::We designed and tested a set of specific primers for specific PCR amplification of the biotin carboxylase subunit gene (accC) of the Acetyl CoA carboxylase (ACCase) enzyme. The primer set yielded a PCR product of c. 460 bp that was suitable for denaturing gradient gel electrophoresis (DGGE) fingerprinting followed by ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01677.x

    authors: Auguet JC,Borrego CM,Bañeras L,Casamayor EO

    更新日期:2008-10-01 00:00:00

  • Energy drives the dynamic localization of cyanobacterial nitrogen regulators during diurnal cycles.

    abstract::Cyanobacteria, phototrophic organisms performing oxygenic photosynthesis, must adapt their metabolic processes to the challenges imposed by the succession of days and nights. Two conserved cyanobacterial proteins, PII and PipX, function as hubs of the nitrogen interaction network, forming complexes with a variety of d...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14071

    authors: Espinosa J,Labella JI,Cantos R,Contreras A

    更新日期:2018-03-01 00:00:00

  • Metagenomic and 14 C tracing evidence for autotrophic microbial CO2 fixation in paddy soils.

    abstract::Autotrophic carbon dioxide (CO2 ) fixation by microbes is ubiquitous in the environment and potentially contributes to the soil organic carbon (SOC) pool. However, the multiple autotrophic pathways of microbial carbon assimilation and fixation in paddy soils remain poorly characterized. In this study, we combine metag...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15204

    authors: Xiao KQ,Ge TD,Wu XH,Peacock CL,Zhu ZK,Peng J,Bao P,Wu JS,Zhu YG

    更新日期:2020-08-21 00:00:00

  • Future warming and acidification result in multiple ecological impacts to a temperate coralline alga.

    abstract::Coralline algae are a crucial component of reef systems, stabilising reef substrate, providing habitat and contributing to accretion. Coralline algae and their surface microbial biofilms are also important as settlement cues for marine invertebrates, yet few studies address the impact of future environmental condition...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14113

    authors: Huggett MJ,McMahon K,Bernasconi R

    更新日期:2018-08-01 00:00:00

  • Elevated levels of VCA0117 (VasH) in response to external signals activate the type VI secretion system of Vibrio cholerae O1 El Tor A1552.

    abstract::The type VI nanomachine is critical for Vibrio cholerae to establish infections and to thrive in niches co-occupied by competing bacteria. The genes for the type VI structural proteins are encoded in one large and two small auxiliary gene clusters. VCA0117 (VasH) - a σ54 -transcriptional activator - is strictly requir...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15141

    authors: Seibt H,Aung KM,Ishikawa T,Sjöström A,Gullberg M,Atkinson GC,Wai SN,Shingler V

    更新日期:2020-10-01 00:00:00

  • A wide host-range metagenomic library from a waste water treatment plant yields a novel alcohol/aldehyde dehydrogenase.

    abstract::Using DNA obtained from the metagenome of an anaerobic digestor in a waste water treatment plant, we constructed a gene library cloned in the wide host-range cosmid pLAFR3. One cosmid enabled Rhizobium leguminosarum to grow on ethanol as sole carbon and energy source, this being due to the presence of a gene, termed a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00854.x

    authors: Wexler M,Bond PL,Richardson DJ,Johnston AW

    更新日期:2005-12-01 00:00:00

  • The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa.

    abstract::LecA (PA-IL) is a cytotoxic lectin and adhesin produced by Pseudomonas aeruginosa which binds hydrophobic galactosides with high specificity and affinity. By using a lecA-egfp translation fusion and immunoblot analysis of the biofilm extracellular matrix, we show that lecA is expressed in biofilm-grown cells. In stati...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.001001.x

    authors: Diggle SP,Stacey RE,Dodd C,Cámara M,Williams P,Winzer K

    更新日期:2006-06-01 00:00:00

  • Competition between microcystin- and non-microcystin-producing Planktothrix agardhii (cyanobacteria) strains under different environmental conditions.

    abstract::The factors that control the production of microcystins (hepatotoxins) during cyanobacterial blooms, and the function of these metabolites remain largely unknown. In an attempt to provide answers to these questions, we compared the fitness of microcystin (MC)-producing and non-MC-producing Planktothrix agardhii strain...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01730.x

    authors: Briand E,Yéprémian C,Humbert JF,Quiblier C

    更新日期:2008-12-01 00:00:00

  • Viral communities associated with healthy and bleaching corals.

    abstract::The coral holobiont is the integrated assemblage of the coral animal, its symbiotic algae, protists, fungi and a diverse consortium of Bacteria and Archaea. Corals are a model system for the study of symbiosis, the breakdown of which can result in disease and mortality. Little is known, however, about viruses that inf...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01652.x

    authors: Marhaver KL,Edwards RA,Rohwer F

    更新日期:2008-09-01 00:00:00

  • Methylation is the initial reaction in anaerobic naphthalene degradation by a sulfate-reducing enrichment culture.

    abstract::The sulfate-reducing culture N47 can utilize naphthalene or 2-methylnaphthalene as the sole carbon source and electron donor. Here we show that the initial reaction in the naphthalene degradation pathway is a methylation to 2-methylnaphthalene which then undergoes the subsequent oxidation to the central metabolite 2-n...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00900.x

    authors: Safinowski M,Meckenstock RU

    更新日期:2006-02-01 00:00:00

  • The faulty SOS response of Pseudomonas putida KT2440 stems from an inefficient RecA-LexA interplay.

    abstract::Despite its environmental robustness Pseudomonas putida strain KT2440 is very sensitive to DNA damage and displays poor homologous recombination efficiencies. To gain an insight into this deficiency isogenic ∆recA and ∆lexA1 derivatives of prophage-free strain P. putida EM173 were generated and responses of the recA a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15384

    authors: Akkaya Ö,Aparicio T,Pérez-Pantoja D,de Lorenzo V

    更新日期:2021-01-03 00:00:00

  • Isolation and gene quantification of heterotrophic N2-fixing bacterioplankton in the Baltic Sea.

    abstract::Cyanobacteria are regarded as the main N(2)-fixing organisms in marine waters. However, recent clone libraries from various oceans show a wide distribution of the dinitrogenase reductase gene (nifH) originating from heterotrophic bacterioplankton. We isolated heterotrophic N(2)-fixing bacteria from Baltic Sea bacterio...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01124.x

    authors: Boström KH,Riemann L,Kühl M,Hagström A

    更新日期:2007-01-01 00:00:00

  • A Pseudomonas putida cardiolipin synthesis mutant exhibits increased sensitivity to drugs related to transport functionality.

    abstract::Biological membranes have evolved different mechanisms to modify their composition in response to chemical stimuli in a process called 'homeoviscous adaptation'. Among these mechanisms, modifications in the ratio of saturated/unsaturated fatty acids and in cis/trans fatty acid isomers, cyclopropanation and changes in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01236.x

    authors: Bernal P,Muñoz-Rojas J,Hurtado A,Ramos JL,Segura A

    更新日期:2007-05-01 00:00:00

  • Alcanivorax which prevails in oil-contaminated seawater exhibits broad substrate specificity for alkane degradation.

    abstract::Alcanivorax is an alkane-degrading marine bacterium which propagates and becomes predominant in crude-oil-containing seawater when nitrogen and phosphorus nutrients are supplemented. In order to understand why Alcanivorax overcomes other bacteria under such cultural conditions, competition experiments between Alcanivo...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1468-2920.2003.00468.x

    authors: Hara A,Syutsubo K,Harayama S

    更新日期:2003-09-01 00:00:00

  • Microbial players involved in the decline of filamentous and colonial cyanobacterial blooms with a focus on fungal parasitism.

    abstract::In the forthcoming decades, it is widely believed that the dominance of colonial and filamentous bloom-forming cyanobacteria (e.g. Microcystis, Planktothrix, Anabaena and Cylindrospermopsis) will increase in freshwater systems as a combined result of anthropogenic nutrient input into freshwater bodies and climate chan...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.12860

    authors: Gerphagnon M,Macarthur DJ,Latour D,Gachon CM,Van Ogtrop F,Gleason FH,Sime-Ngando T

    更新日期:2015-08-01 00:00:00

  • Rapid and highly efficient genomic engineering with a novel iEditing device for programming versatile extracellular electron transfer of electroactive bacteria.

    abstract::The advances in synthetic biology bring exciting new opportunities to reprogram microorganisms with novel functionalities for environmental applications. For real-world applications, a genetic tool that enables genetic engineering in a stably genomic inherited manner is greatly desired. In this work, we design a novel...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15374

    authors: Fan YY,Tang Q,Li Y,Li FH,Wu JH,Li WW,Yu HQ

    更新日期:2020-12-28 00:00:00

  • Metabolic biomarkers for monitoring anaerobic naphthalene biodegradation in situ.

    abstract::During the anaerobic biodegradation of naphthalene and methylnaphthalene, unique metabolites are formed by specific microbial carboxylation and ring-reduction reactions. Groundwater samples from an anoxic, shallow aquifer contaminated with gasoline were examined for the presence of these metabolites by extraction, der...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00330.x

    authors: Phelps CD,Battistelli J,Young LY

    更新日期:2002-09-01 00:00:00

  • Elements of the heterocyst-specific transcriptome unravelled by co-expression analysis in Nostoc sp. PCC 7120.

    abstract::Nitrogen is frequently limiting microbial growth in the environment. As a response, many filamentous cyanobacteria differentiate heterocysts, cells devoted to N2 fixation. Heterocyst differentiation is under the control of the master regulator HetR. Through the characterization of the HetR-dependent transcriptome in N...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14647

    authors: Brenes-Álvarez M,Mitschke J,Olmedo-Verd E,Georg J,Hess WR,Vioque A,Muro-Pastor AM

    更新日期:2019-07-01 00:00:00