Metabolic biomarkers for monitoring anaerobic naphthalene biodegradation in situ.

Abstract:

:During the anaerobic biodegradation of naphthalene and methylnaphthalene, unique metabolites are formed by specific microbial carboxylation and ring-reduction reactions. Groundwater samples from an anoxic, shallow aquifer contaminated with gasoline were examined for the presence of these metabolites by extraction, derivatization and gas chromatography coupled to mass spectroscopy. Several metabolites [2-naphthoic acid (2-NA), tetrahydro-2-naphthoic acid (TH-2-NA), hexahydro-2-naphthoic acid (HH-2-NA) and methylnaphthoic acid (MNA)] were found to be present in the groundwater samples. The concentration of 2-NA at each monitoring well was quantified and correlated to the zones of contamination. The presence of the other metabolites in the same wells as 2-NA was used as confirmation that the anaerobic pathway was indeed active. The distribution of metabolites at this site shows that they can be used as biomarkers for demonstrating in situ biodegradation.

journal_name

Environ Microbiol

authors

Phelps CD,Battistelli J,Young LY

doi

10.1046/j.1462-2920.2002.00330.x

keywords:

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

532-7

issue

9

eissn

1462-2912

issn

1462-2920

pii

330

journal_volume

4

pub_type

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