A diverse uncultivated microbial community is responsible for organic matter degradation in the Black Sea sulphidic zone.

Abstract:

:Organic matter degradation in marine environments is essential for the recycling of nutrients, especially under conditions of anoxia where organic matter tends to accumulate. However, little is known about the diversity of the microbial communities responsible for the mineralization of organic matter in the absence of oxygen, as well as the factors controlling their activities. Here, we determined the active heterotrophic prokaryotic community in the sulphidic water column of the Black Sea, an ideal model system, where a tight coupling between carbon, nitrogen and sulphur cycles is expected. Active microorganisms degrading both dissolved organic matter (DOM) and protein extracts were determined using quantitative DNA stable isotope probing incubation experiments. These results were compared with the metabolic potential of metagenome-assembled genomes obtained from the water column. Organic matter incubations showed that groups like Cloacimonetes and Marinimicrobia are generalists degrading DOM. Based on metagenomic profiles the degradation proceeds in a potential interaction with members of the Deltaproteobacteria and Chloroflexi Dehalococcoidia. On the other hand, microbes with small genomes like the bacterial phyla Parcubacteria, Omnitrophica and of the archaeal phylum Woesearchaeota, were the most active, especially in protein-amended incubations, revealing the potential advantage of streamlined microorganisms in highly reduced conditions.

journal_name

Environ Microbiol

authors

Suominen S,Dombrowski N,Sinninghe Damsté JS,Villanueva L

doi

10.1111/1462-2920.14902

subject

Has Abstract

pub_date

2019-12-19 00:00:00

eissn

1462-2912

issn

1462-2920

pub_type

杂志文章
  • Design and validation of 16S rRNA probes to enumerate members of the Clostridium leptum subgroup in human faecal microbiota.

    abstract::Among human faecal bacteria, many members of the Clostridium leptum subgroup are fibrolytic and butyrate producing microorganisms thereby contributing to processes important to colonic health. Yet this phylogenetic subgroup remains poorly described to date. To improve detection and description of members of the C. lep...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00763.x

    authors: Lay C,Sutren M,Rochet V,Saunier K,Doré J,Rigottier-Gois L

    更新日期:2005-07-01 00:00:00

  • Nitrite concentration influences the population structure of Nitrospira-like bacteria.

    abstract::Chemolithoautotrophic nitrite oxidizers of the genus Nitrospira are a monophyletic but diverse group of organisms, are widely distributed in many natural habitats, and play a key role in nitrogen elimination during biological wastewater treatment. Phylogenetic analyses of cloned 16S rRNA genes and fluorescence in situ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01033.x

    authors: Maixner F,Noguera DR,Anneser B,Stoecker K,Wegl G,Wagner M,Daims H

    更新日期:2006-08-01 00:00:00

  • Quorum sensing regulates 'swim-or-stick' lifestyle in the phycosphere.

    abstract::Interactions between phytoplankton and bacteria play major roles in global biogeochemical cycles and oceanic nutrient fluxes. These interactions occur in the microenvironment surrounding phytoplankton cells, known as the phycosphere. Bacteria in the phycosphere use either chemotaxis or attachment to benefit from algal...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15228

    authors: Fei C,Ochsenkühn MA,Shibl AA,Isaac A,Wang C,Amin SA

    更新日期:2020-11-01 00:00:00

  • Quantitative PCR reveals transient and persistent algal viruses in Lake Ontario, Canada.

    abstract::To determine if different algal viruses (Phycodnaviridae) share common patterns of seasonal abundance, quantitative PCR methods were developed and applied to monitor the abundances of three different viruses in Lake Ontario, Canada over 13 months. Throughout the year, the abundances of two different phycodnavirus polB...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01988.x

    authors: Short SM,Short CM

    更新日期:2009-10-01 00:00:00

  • Genomic insights into the Acidobacteria reveal strategies for their success in terrestrial environments.

    abstract::Members of the phylum Acidobacteria are abundant and ubiquitous across soils. We performed a large-scale comparative genome analysis spanning subdivisions 1, 3, 4, 6, 8 and 23 (n = 24) with the goal to identify features to help explain their prevalence in soils and understand their ecophysiology. Our analysis revealed...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14043

    authors: Eichorst SA,Trojan D,Roux S,Herbold C,Rattei T,Woebken D

    更新日期:2018-03-01 00:00:00

  • Ectomycorrhizal fungi inoculation alleviates simulated acid rain effects on soil ammonia oxidizers and denitrifiers in Masson pine forest.

    abstract::Acid rain can cause severe effects on soil biota and nutrient biogeochemical cycles in the forest ecosystem, but how plant-symbiotic ectomycorrhizal fungi will modulate the effects remains unknown. Here, we conducted a full factorial field experiment in a Masson pine forest by simultaneously controlling the acidity of...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14457

    authors: Li Y,Chen Z,He JZ,Wang Q,Shen C,Ge Y

    更新日期:2019-01-01 00:00:00

  • Photosynthetic performance of phototrophic biofilms in extreme acidic environments.

    abstract::Photosynthesis versus irradiance curves and their associated photosynthetic parameters from different phototrophic biofilms isolated from an extreme acidic environment (Río Tinto, SW, Spain) were studied in order to relate them to their species composition and the physicochemical characteristics of their respective sa...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02506.x

    authors: Souza-Egipsy V,Altamirano M,Amils R,Aguilera A

    更新日期:2011-08-01 00:00:00

  • Comparative proteomics of the toxigenic diazotroph Raphidiopsis raciborskii (cyanobacteria) in response to iron.

    abstract::Raphidiopsis raciborskii is an invasive bloom-forming cyanobacteria with the flexibility to utilize atmospheric and fixed nitrogen. Since nitrogen-fixation has a high requirement for iron as an ezyme cofactor, we hypothesize that iron availability would determine the success of the species under nitrogen-fixing condit...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15328

    authors: D'Agostino PM,Yeung ACY,Poljak A,David Waite T,Neilan BA

    更新日期:2021-01-01 00:00:00

  • High bacterial diversity of a waste gas-degrading community in an industrial biofilter as shown by a 16S rDNA clone library.

    abstract::The bacterial diversity of an industrial biofilter used for waste gas abatement in an animal-rendering plant was investigated. A 16S rDNA clone library was generated and 444 clones were screened using computer-aided amplified ribosomal DNA restriction analysis (ARDRA). Of the screened clones, 60.8% showed unique ARDRA...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00349.x

    authors: Friedrich U,Prior K,Altendorf K,Lipski A

    更新日期:2002-11-01 00:00:00

  • Fungal transformation of selenium and tellurium located in a volcanogenic sulfide deposit.

    abstract::Microbial reduction of soluble selenium (Se) or tellurium (Te) species results in immobilization as elemental forms and this process has been employed in soil bioremediation. However, little is known of direct and indirect fungal interactions with Se-/Te-bearing ores. In this research, the ability of Phoma glomerata t...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15012

    authors: Liang X,Perez MAM,Zhang S,Song W,Armstrong JG,Bullock LA,Feldmann J,Parnell J,Csetenyi L,Gadd GM

    更新日期:2020-06-01 00:00:00

  • EukRef-Ciliophora: a manually curated, phylogeny-based database of small subunit rRNA gene sequences of ciliates.

    abstract::High-throughput sequencing (HTS) surveys, among the most common approaches currently used in environmental microbiology, require reliable reference databases to be correctly interpreted. The EukRef Initiative (eukref.org) is a community effort to manually screen available small subunit (SSU) rRNA gene sequences and pr...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14264

    authors: Boscaro V,Santoferrara LF,Zhang Q,Gentekaki E,Syberg-Olsen MJ,Del Campo J,Keeling PJ

    更新日期:2018-06-01 00:00:00

  • Structure and function of high Arctic pelagic, particle-associated and benthic bacterial communities.

    abstract::Arctic marine microbes are affected by environmental changes that may ultimately influence their functions in carbon cycling. Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to eva...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14304

    authors: Balmonte JP,Teske A,Arnosti C

    更新日期:2018-08-01 00:00:00

  • Basin-scale distribution patterns of picocyanobacterial lineages in the Atlantic Ocean.

    abstract::Marine picocyanobacteria of the genera Prochlorococcus and Synechococcus are major contributors to oceanic primary production. The genera are genetically diverse, comprising several known ecotypes or lineages. However, little is known of the distribution of these lineages over large geographic areas. Here, we analysed...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01246.x

    authors: Zwirglmaier K,Heywood JL,Chamberlain K,Woodward EM,Zubkov MV,Scanlan DJ

    更新日期:2007-05-01 00:00:00

  • Type 4 pili are dispensable for biofilm development in the cyanobacterium Synechococcus elongatus.

    abstract::The hair-like cell appendages denoted as type IV pili are crucial for biofilm formation in diverse eubacteria. The protein complex responsible for type IV pilus assembly is homologous with the type II protein secretion complex. In the cyanobacterium Synechococcus elongatus PCC 7942, the gene Synpcc7942_2071 encodes an...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13814

    authors: Nagar E,Zilberman S,Sendersky E,Simkovsky R,Shimoni E,Gershtein D,Herzberg M,Golden SS,Schwarz R

    更新日期:2017-07-01 00:00:00

  • Escherichia coli has multiple enzymes that attack TNT and release nitrogen for growth.

    abstract::There has been a growing interest in the degradation of 2,4,6-trinitrotoluene (TNT) over the last decade, ever since its removal from polluted sites was declared an international environmental priority. Certain aerobic and anaerobic microorganisms are capable of using TNT as an N source, although very few studies have...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01272.x

    authors: González-Pérez MM,van Dillewijn P,Wittich RM,Ramos JL

    更新日期:2007-06-01 00:00:00

  • Detection of glycolate oxidase gene glcD diversity among cultured and environmental marine bacteria.

    abstract::Of eight laboratory cultures of marine gamma- and alpha-Proteobacteria tested, growth on glycolate as a sole carbon source was detected for only three species: Pseudomonas stutzeri, Oceanimonas doudoroffii and Roseobacter sp. isolate Y3F. Degenerate polymerase chain reaction (PCR) primers were designed to amplify glcD...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01092.x

    authors: Lau WW,Armbrust EV

    更新日期:2006-10-01 00:00:00

  • Tn5530 from Burkholderia cepacia strain 2a encodes a chloride channel protein essential for the catabolism of 2,4-dichlorophenoxyacetic acid.

    abstract::Chloride channel proteins (ClC) are found in living systems where they transport chloride ions across cell membranes. Recently, the structure/function of two prokaryotic ClC has been determined but little is known about the role of these proteins in the microbial metabolism of chlorinated compounds. Here we show that ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,收录出版

    doi:10.1111/j.1462-2920.2006.01136.x

    authors: Sebastianelli A,Bruce IJ

    更新日期:2007-01-01 00:00:00

  • Effects of uranium concentration on microbial community structure and functional potential.

    abstract::Located in the Northern Territory of Australia, Ranger uranium mine is directly adjacent to the UNESCO World Heritage listed Kakadu National Park, with rehabilitation targets needed to ensure the site can be incorporated into the park following the mine's closure in 2026. This study aimed to understand the impact of u...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13839

    authors: Sutcliffe B,Chariton AA,Harford AJ,Hose GC,Greenfield P,Elbourne LDH,Oytam Y,Stephenson S,Midgley DJ,Paulsen IT

    更新日期:2017-08-01 00:00:00

  • Artificial construction of the biocoenosis of deep-sea ecosystem via seeping methane.

    abstract::Deep-sea ecosystems, such as cold seeps and hydrothermal vents, have high biomass, even though they are located in the benthic zone, where no sunlight is present to provide energy for organism proliferation. Based on the coexistence of the reduced gases and chemoautotrophic microbes, it is inferred that the energy fro...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15347

    authors: Sun X,Yang J,Zheng M,Zhang X

    更新日期:2020-12-07 00:00:00

  • Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia.

    abstract::Concatenated sequence analysis with 16S rRNA, rpoB and fusA genes identified a bacterial strain (IRBG74) isolated from root nodules of the aquatic legume Sesbania cannabina as a close relative of the plant pathogen Rhizobium radiobacter (syn. Agrobacterium tumefaciens). However, DNA:DNA hybridization with R. radiobact...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01975.x

    authors: Cummings SP,Gyaneshwar P,Vinuesa P,Farruggia FT,Andrews M,Humphry D,Elliott GN,Nelson A,Orr C,Pettitt D,Shah GR,Santos SR,Krishnan HB,Odee D,Moreira FM,Sprent JI,Young JP,James EK

    更新日期:2009-10-01 00:00:00

  • Characterization of PfiT/PfiA toxin-antitoxin system of Pseudomonas aeruginosa that affects cell elongation and prophage induction.

    abstract::Toxin-antitoxin (TA) systems are small genetic modules usually consisting of two elements-a toxin and an antitoxin. The abundance of TA systems among various bacterial strains may indicate an important evolutionary role. Pseudomonas aeruginosa, which can be found in a variety of niches in nature, is an opportunistic p...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15102

    authors: Zander I,Shmidov E,Roth S,Ben-David Y,Shoval I,Shoshani S,Danielli A,Banin E

    更新日期:2020-12-01 00:00:00

  • Non-canonical localization of RubisCO under high-light conditions in the toxic cyanobacterium Microcystis aeruginosa PCC7806.

    abstract::The frequent production of the hepatotoxin microcystin (MC) and its impact on the lifestyle of bloom-forming cyanobacteria are poorly understood. Here, we report that MC interferes with the assembly and the subcellular localization of RubisCO, in Microcystis aeruginosa PCC7806. Immunofluorescence, electron microscopic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14837

    authors: Barchewitz T,Guljamow A,Meissner S,Timm S,Henneberg M,Baumann O,Hagemann M,Dittmann E

    更新日期:2019-12-01 00:00:00

  • Surveying biotransformations with à la carte genetic traps: translating dehydrochlorination of lindane (gamma-hexachlorocyclohexane) into lacZ-based phenotypes.

    abstract::The ability of the product of a desired reaction to activate a bacterial transcriptional regulator was exploited to develop genetic traps that render the catalytic activity born by a DNA clone into a selectable/scorable phenotype. We established this strategy with a system to expose the activity of dehydrochlorinases ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.00983.x

    authors: Mohn WW,Garmendia J,Galvao TC,de Lorenzo V

    更新日期:2006-03-01 00:00:00

  • Expression and regulation of the mer operon in Thermus thermophilus.

    abstract::Mercury (Hg) is a highly toxic and widely distributed heavy metal, which some Bacteria and Archaea detoxify by the reduction of ionic Hg (Hg[II]) to the elemental volatile form, Hg(0). This activity is specified by the mer operon. The mer operon of the deeply branching thermophile Thermus thermophilus HB27 encodes for...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14953

    authors: Norambuena J,Miller M,Boyd JM,Barkay T

    更新日期:2020-04-01 00:00:00

  • Unravelling lactate-acetate and sugar conversion into butyrate by intestinal Anaerobutyricum and Anaerostipes species by comparative proteogenomics.

    abstract::The d- and l-forms of lactate are important fermentation metabolites produced by intestinal bacteria but are found to negatively affect mucosal barrier function and human health. Both enantiomers of lactate can be converted with acetate into the presumed beneficial butyrate by a phylogenetically related group of anaer...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15269

    authors: Shetty SA,Boeren S,Bui TPN,Smidt H,de Vos WM

    更新日期:2020-11-01 00:00:00

  • A molecular assessment of the iron stress response in the two phylogenetic clades of Trichodesmium.

    abstract::Trichodesmium spp. play key roles in global carbon and nitrogen budgets and thus defining what controls their productivity is important for understanding climate change. While iron availability has been shown to be an important chemical factor for controlling both growth and nitrogen fixation rates in Trichodesmium, a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02026.x

    authors: Chappell PD,Webb EA

    更新日期:2010-01-01 00:00:00

  • Introducing insoluble wheat bran as a gut microbiota niche in an in vitro dynamic gut model stimulates propionate and butyrate production and induces colon region specific shifts in the luminal and mucosal microbial community.

    abstract::The spatial organization of gut microorganisms is important with respect to their functional role in the gut ecosystem. Regional differences in the longitudinal and lateral direction are, however, not frequently studied, given the difficulty to sample these human gut regions in vivo. Particularly the insoluble food pa...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14381

    authors: De Paepe K,Verspreet J,Verbeke K,Raes J,Courtin CM,Van de Wiele T

    更新日期:2018-09-01 00:00:00

  • The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina.

    abstract::Reactive oxygen species (ROS) play an important role during host-pathogen interactions and are often an indication of induced host defence responses. In this study, we demonstrate for the first time that Puccinia triticina (Pt) generates ROS, including superoxide, H2 O2 and hydroxyl radicals, during wheat infection. T...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15063

    authors: Wang X,Che MZ,Khalil HB,McCallum BD,Bakkeren G,Rampitsch C,Saville BJ

    更新日期:2020-07-01 00:00:00

  • Functional and structural response of a cellulose-degrading methanogenic microbial community to multiple aeration stress at two different temperatures.

    abstract::Two cellulose-fermenting methanogenic enrichment cultures originating from rice soil, one at 15 degrees C with Methanosaeta and the other at 30 degrees C with Methanosarcina as the dominant acetoclastic methanogen, both degraded cellulose anaerobically via propionate, acetate and H2 to CH4. The degradation was a two-s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00199.x

    authors: Wu XL,Conrad R

    更新日期:2001-06-01 00:00:00

  • Characterization of two alkane hydroxylase genes from the marine hydrocarbonoclastic bacterium Alcanivorax borkumensis.

    abstract::The marine gamma-Proteobacterium Alcanivorax borkumensis is highly specialized in the assimilation of aliphatic hydrocarbons, and makes up a large part of the biomass in oil-polluted marine environments. In addition to the previously identified alkane hydroxylase AlkB1, a second alkane hydroxylase (AlkB2) showing 65% ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00567.x

    authors: van Beilen JB,Marín MM,Smits TH,Röthlisberger M,Franchini AG,Witholt B,Rojo F

    更新日期:2004-03-01 00:00:00