Type 4 pili are dispensable for biofilm development in the cyanobacterium Synechococcus elongatus.

Abstract:

:The hair-like cell appendages denoted as type IV pili are crucial for biofilm formation in diverse eubacteria. The protein complex responsible for type IV pilus assembly is homologous with the type II protein secretion complex. In the cyanobacterium Synechococcus elongatus PCC 7942, the gene Synpcc7942_2071 encodes an ATPase homologue of type II/type IV systems. Here, we report that inactivation of Synpcc7942_2071 strongly affected the suite of proteins present in the extracellular milieu (exo-proteome) and eliminated pili observable by electron microscopy. These results support a role for this gene product in protein secretion as well as in pili formation. As we previously reported, inactivation of Synpcc7942_2071 enables biofilm formation and suppresses the planktonic growth of S. elongatus. Thus, pili are dispensable for biofilm development in this cyanobacterium, in contrast to their biofilm-promoting function in type IV pili-producing heterotrophic bacteria. Nevertheless, pili removal is not required for biofilm formation as evident by a piliated mutant of S. elongatus that develops biofilms. We show that adhesion and timing of biofilm development differ between the piliated and non-piliated strains. The study demonstrates key differences in the process of biofilm formation between cyanobacteria and well-studied type IV pili-producing heterotrophic bacteria.

journal_name

Environ Microbiol

authors

Nagar E,Zilberman S,Sendersky E,Simkovsky R,Shimoni E,Gershtein D,Herzberg M,Golden SS,Schwarz R

doi

10.1111/1462-2920.13814

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

2862-2872

issue

7

eissn

1462-2912

issn

1462-2920

journal_volume

19

pub_type

杂志文章
  • The Trojan Horse of the microbiological arms race: phage-encoded toxins as a defence against eukaryotic predators.

    abstract::Phage-encoded Shiga toxin (Stx) acts as a bacterial defence against the eukaryotic predator Tetrahymena. To function as an effective bacterial anti-predator defence, Stx must kill a broad spectrum of predators. Consistent with that assertion, we show here that bacterially encoded Stx efficiently kills the bacteriovore...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12232

    authors: Arnold JW,Koudelka GB

    更新日期:2014-02-01 00:00:00

  • Ecological and genomic features of two widespread freshwater picocyanobacteria.

    abstract::We present two genomes of widespread freshwater picocyanobacteria isolated by extinction dilution from a Spanish oligotrophic reservoir. Based on microscopy and genomic properties, both picocyanobacteria were tentatively designated Synechococcus lacustris Tous, formerly described as a metagenome assembled genome (MAG)...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14377

    authors: Cabello-Yeves PJ,Picazo A,Camacho A,Callieri C,Rosselli R,Roda-Garcia JJ,Coutinho FH,Rodriguez-Valera F

    更新日期:2018-10-01 00:00:00

  • Oligotrophic lagoons of the South Pacific Ocean are home to a surprising number of novel eukaryotic microorganisms.

    abstract::The diversity of microbial eukaryotes was surveyed by environmental sequencing from tropical lagoon sites of the South Pacific, collected through the American Museum of Natural History (AMNH)'s Explore21 expedition to the Solomon Islands in September 2013. The sampled lagoons presented low nutrient concentrations typi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13523

    authors: Kim E,Sprung B,Duhamel S,Filardi C,Kyoon Shin M

    更新日期:2016-12-01 00:00:00

  • Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia.

    abstract::Concatenated sequence analysis with 16S rRNA, rpoB and fusA genes identified a bacterial strain (IRBG74) isolated from root nodules of the aquatic legume Sesbania cannabina as a close relative of the plant pathogen Rhizobium radiobacter (syn. Agrobacterium tumefaciens). However, DNA:DNA hybridization with R. radiobact...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01975.x

    authors: Cummings SP,Gyaneshwar P,Vinuesa P,Farruggia FT,Andrews M,Humphry D,Elliott GN,Nelson A,Orr C,Pettitt D,Shah GR,Santos SR,Krishnan HB,Odee D,Moreira FM,Sprent JI,Young JP,James EK

    更新日期:2009-10-01 00:00:00

  • Respiratory quinones in Archaea: phylogenetic distribution and application as biomarkers in the marine environment.

    abstract::The distribution of respiratory quinone electron carriers among cultivated organisms provides clues on both the taxonomy of their producers and the redox processes these are mediating. Our study of the quinone inventories of 25 archaeal species belonging to the phyla Eury-, Cren- and Thaumarchaeota facilitates their u...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13086

    authors: Elling FJ,Becker KW,Könneke M,Schröder JM,Kellermann MY,Thomm M,Hinrichs KU

    更新日期:2016-02-01 00:00:00

  • Diversity patterns of Rhizobiaceae communities inhabiting soils, root surfaces and nodules reveal a strong selection of rhizobial partners by legumes.

    abstract::Current knowledge about rhizobial diversity patterns in non-nodule habitats is scarce, limiting our understanding of basic aspects of rhizobial ecology like competitiveness for nodule occupancy and host effects on community structure. We used a combination of cultivation-dependent and independent approaches to analyse...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13061

    authors: Miranda-Sánchez F,Rivera J,Vinuesa P

    更新日期:2016-09-01 00:00:00

  • In situ survey of life cycle phases of the coccolithophore Emiliania huxleyi (Haptophyta).

    abstract::The cosmopolitan coccolithophore Emiliania huxleyi is characterized by a strongly differentiated haplodiplontic life cycle consisting of a diploid phase, generally bearing coccoliths (calcified) but that can be also non-calcified, and a non-calcified biflagellated haploid phase. Given most studies have focused on the ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02745.x

    authors: Frada MJ,Bidle KD,Probert I,de Vargas C

    更新日期:2012-06-01 00:00:00

  • Structural and functional patterns of bacterial communities in response to protist predation along an experimental productivity gradient.

    abstract::Substrate supply and protist grazing are two of the most important forces that determine the composition and properties of bacterial assemblages. General ecological theory predicts that the relative importance of these factors is changing with the environmental productivity. In the present study, the interplay between...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01713.x

    authors: Corno G,Jürgens K

    更新日期:2008-10-01 00:00:00

  • Microbial players involved in the decline of filamentous and colonial cyanobacterial blooms with a focus on fungal parasitism.

    abstract::In the forthcoming decades, it is widely believed that the dominance of colonial and filamentous bloom-forming cyanobacteria (e.g. Microcystis, Planktothrix, Anabaena and Cylindrospermopsis) will increase in freshwater systems as a combined result of anthropogenic nutrient input into freshwater bodies and climate chan...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.12860

    authors: Gerphagnon M,Macarthur DJ,Latour D,Gachon CM,Van Ogtrop F,Gleason FH,Sime-Ngando T

    更新日期:2015-08-01 00:00:00

  • In vitro and in vivo expression of virulence genes in Vibrio isolates belonging to the Harveyi clade in relation to their virulence towards gnotobiotic brine shrimp (Artemia franciscana).

    abstract::Vibrios belonging to the Harveyi clade are pathogenic marine bacteria affecting both vertebrates and invertebrates, thereby causing a severe threat to the aquaculture industry. In this study, the expression of haemolysin, metalloprotease, serine protease, the quorum sensing master regulator LuxR and the virulence regu...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02354.x

    authors: Ruwandeepika HA,Defoirdt T,Bhowmick PP,Karunasagar I,Karunasagar I,Bossier P

    更新日期:2011-02-01 00:00:00

  • Sub-seafloor biogeochemical processes and microbial life in the Baltic Sea.

    abstract::The post-glacial Baltic Sea has experienced extreme changes that are archived today in the deep sediments. IODP Expedition 347 retrieved cores down to 100 m depth and studied the climate history and the deep biosphere. We here review the biogeochemical and microbiological highlights and integrate these with other stud...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.14920

    authors: Jørgensen BB,Andrén T,Marshall IPG

    更新日期:2020-05-01 00:00:00

  • Links between ammonia oxidizer species composition, functional diversity and nitrification kinetics in grassland soils.

    abstract::Molecular approaches have revealed considerable diversity and uncultured novelty in natural prokaryotic populations, but not direct links between the new genotypes detected and ecosystem processes. Here we describe the influence of the structure of communities of ammonia-oxidizing bacteria on nitrogen cycling in micro...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00740.x

    authors: Webster G,Embley TM,Freitag TE,Smith Z,Prosser JI

    更新日期:2005-05-01 00:00:00

  • 'Candidatus Lumbricincola', a novel lineage of uncultured Mollicutes from earthworms of family Lumbricidae.

    abstract::Molecular signatures of new, as yet uncultured mollicute-like organisms (MLOs) have been detected in total rRNA and DNA extracted from tissues, gut contents and casts of four species of the earthworm family Lumbricidae. The MLO 16S rRNA sequences exhibited low identity to those of known Mollicutes species and formed a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01837.x

    authors: Nechitaylo TY,Timmis KN,Golyshin PN

    更新日期:2009-04-01 00:00:00

  • The arbuscular mycorrhizal fungus Rhizophagus irregularis uses a reductive iron assimilation pathway for high-affinity iron uptake.

    abstract::Arbuscular mycorrhizal (AM) fungi can improve iron (Fe) acquisition of their host plants. Here, we report a characterization of two components of the high-affinity reductive Fe uptake system of Rhizophagus irregularis, the ferric reductase (RiFRE1) and the high affinity Fe permeases (RiFTR1-2). In the extraradical myc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14121

    authors: Tamayo E,Knight SAB,Valderas A,Dancis A,Ferrol N

    更新日期:2018-05-01 00:00:00

  • Recognition cascade and metabolite transfer in a marine bacteria-phytoplankton model system.

    abstract::The trophic linkage between marine bacteria and phytoplankton in the surface ocean is a key step in the global carbon cycle, with almost half of marine primary production transformed by heterotrophic bacterioplankton within hours to weeks of fixation. Early studies conceptualized this link as the passive addition and ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13834

    authors: Durham BP,Dearth SP,Sharma S,Amin SA,Smith CB,Campagna SR,Armbrust EV,Moran MA

    更新日期:2017-09-01 00:00:00

  • Double-tagged fluorescent bacterial bioreporter for the study of polycyclic aromatic hydrocarbon diffusion and bioavailability.

    abstract::Bacterial degradation of polycyclic aromatic hydrocarbons (PAHs), ubiquitous contaminants from oil and coal, is typically limited by poor accessibility of the contaminant to the bacteria. In order to measure PAH availability in complex systems, we designed a number of diffusion-based assays with a double-tagged bacter...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01952.x

    authors: Tecon R,Binggeli O,van der Meer JR

    更新日期:2009-09-01 00:00:00

  • Microbial community associated with the colonial ascidian Cystodytes dellechiajei.

    abstract::The ascidian Cystodytes dellechiajei (Della Valle, 1877) (phylum Chordata, class Ascidiacea, family Polycitoridae) is a colonial tunicate that inhabits benthic rock environments in the Atlantic, Pacific and Indian Oceans, as well as the Mediterranean Sea. Its life cycle has two phases, the adult sessile colony and the...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01170.x

    authors: Martínez-García M,Díaz-Valdés M,Wanner G,Ramos-Esplá A,Antón J

    更新日期:2007-02-01 00:00:00

  • Methanol metabolism in the acetogenic bacterium Acetobacterium woodii.

    abstract::Methanol derived from plant tissue is ubiquitous in anaerobic sediments and a good substrate for anaerobes growing on C1 compounds such as methanogens and acetogens. In contrast to methanogens little is known about the physiology, biochemistry and bioenergetics of methanol utilization in acetogenic bacteria. To fill t...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14356

    authors: Kremp F,Poehlein A,Daniel R,Müller V

    更新日期:2018-12-01 00:00:00

  • Persister cells resuscitate via ribosome modification by 23S rRNA pseudouridine synthase RluD.

    abstract::Upon a wide range of stress conditions (e.g. nutrient, antibiotic, oxidative), a subpopulation of bacterial cells known as persisters survives by halting metabolism. These cells resuscitate rapidly to reconstitute infections once the stress is removed and nutrients are provided. However, how these dormant cells resusc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14828

    authors: Song S,Wood TK

    更新日期:2020-03-01 00:00:00

  • Kleptoplasty in an Antarctic dinoflagellate: caught in evolutionary transition?

    abstract::Photosynthetic dinoflagellates contain a diverse collection of plastid types, a situation believed to have arisen from multiple endosymbiotic events. In addition, a number of heterotrophic (phagotrophic) dinoflagellates possess the ability to acquire chloroplasts temporarily by engulfing algae and retaining their chlo...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01109.x

    authors: Gast RJ,Moran DM,Dennett MR,Caron DA

    更新日期:2007-01-01 00:00:00

  • Isotope analysis as a natural reaction probe to determine mechanisms of biodegradation of 1,2-dichloroethane.

    abstract::1,2-Dichloroethane (1,2-DCA), a chlorinated aliphatic hydrocarbon, is a well-known groundwater contaminant. In this study, fractionation of stable carbon isotope values of 1,2-DCA during biodegradation was used as a novel reaction probe to provide information about the mechanism of 1,2-DCA biodegradation under both ae...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01282.x

    authors: Hirschorn SK,Dinglasan-Panlilio MJ,Edwards EA,Lacrampe-Couloume G,Sherwood Lollar B

    更新日期:2007-07-01 00:00:00

  • Bacterial biogeography in the coastal waters of northern Zhejiang, East China Sea is highly controlled by spatially structured environmental gradients.

    abstract::The underlying mechanisms of microbial community assembly in connective coastal environments are unclear. The coastal water area of northern Zhejiang, East China Sea, is a complex marine ecosystem with multiple environmental gradients, where the distributions and determinants of bacterioplankton communities remain unc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12884

    authors: Wang K,Ye X,Chen H,Zhao Q,Hu C,He J,Qian Y,Xiong J,Zhu J,Zhang D

    更新日期:2015-10-01 00:00:00

  • Thioquinolobactin, a Pseudomonas siderophore with antifungal and anti-Pythium activity.

    abstract::Under conditions of iron limitation Pseudomonas fluorescens ATCC 17400 produces two siderophores, pyoverdine, and a second siderophore quinolobactin, which itself results from the hydrolysis of the unstable molecule 8-hydroxy-4-methoxy-2-quinoline thiocarboxylic acid (thioquinolobactin). Pseudomonas fluorescens ATCC 1...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01154.x

    authors: Matthijs S,Tehrani KA,Laus G,Jackson RW,Cooper RM,Cornelis P

    更新日期:2007-02-01 00:00:00

  • The biogeography of Streptomyces in New Zealand enabled by high-throughput sequencing of genus-specific rpoB amplicons.

    abstract::We evaluated Streptomyces biogeography in soils along a 1200 km latitudinal transect across New Zealand (NZ). Streptomyces diversity was examined using high-throughput sequencing of rpoB amplicons generated with a Streptomyces specific primer set. We detected 1287 Streptomyces rpoB operational taxonomic units (OTUs) w...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15350

    authors: Higgins SA,Panke-Buisse K,Buckley DH

    更新日期:2020-12-07 00:00:00

  • Novel Caudovirales associated with Marine Group I Thaumarchaeota assembled from metagenomes.

    abstract::Marine Group I (MGI) Thaumarchaeota are some of the most abundant microorganisms in the deep ocean and responsible for much of the ammonia oxidation occurring in this environment. In this work, we present 35 sequences assembled from metagenomic samples of the first uncultivated Caudovirales viruses associated with Tha...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14462

    authors: López-Pérez M,Haro-Moreno JM,de la Torre JR,Rodriguez-Valera F

    更新日期:2019-06-01 00:00:00

  • A diverse uncultivated microbial community is responsible for organic matter degradation in the Black Sea sulphidic zone.

    abstract::Organic matter degradation in marine environments is essential for the recycling of nutrients, especially under conditions of anoxia where organic matter tends to accumulate. However, little is known about the diversity of the microbial communities responsible for the mineralization of organic matter in the absence of...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14902

    authors: Suominen S,Dombrowski N,Sinninghe Damsté JS,Villanueva L

    更新日期:2019-12-19 00:00:00

  • Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage.

    abstract::Cultivation-independent surveys of ribosomal RNA genes have revealed the existence of novel microbial lineages, many with no known cultivated representatives. Ribosomal RNA-based analyses, however, often do not provide significant information beyond phylogenetic affiliation. Analysis of large genome fragments recovere...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2000.00133.x

    authors: Béjà O,Suzuki MT,Koonin EV,Aravind L,Hadd A,Nguyen LP,Villacorta R,Amjadi M,Garrigues C,Jovanovich SB,Feldman RA,DeLong EF

    更新日期:2000-10-01 00:00:00

  • Construction, characterization, and use of small-insert gene banks of DNA isolated from soil and enrichment cultures for the recovery of novel amidases.

    abstract::To obtain new amidases of biocatalytic relevance, we used microorganisms indigenous to different types of soil and sediment as a source of DNA for the construction of environmental gene banks, following two different strategies. In one case, DNA was isolated from soil without preceding cultivation to preserve a high d...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00643.x

    authors: Gabor EM,de Vries EJ,Janssen DB

    更新日期:2004-09-01 00:00:00

  • Susceptibility of Vibrio aestuarianus 01/032 to the antibacterial activity of Mytilus haemolymph: identification of a serum opsonin involved in mannose-sensitive interactions.

    abstract::The interactions of Vibrio aestuarianus 01/032 with haemolymph of the bivalves Mytilus galloprovincialis and Crassostrea gigas were investigated to understand if haemolymph components (haemocytes and soluble factors) could be involved in the higher resistance to microbial infection shown by mussels in comparison with ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12750

    authors: Pezzati E,Canesi L,Damonte G,Salis A,Marsano F,Grande C,Vezzulli L,Pruzzo C

    更新日期:2015-11-01 00:00:00

  • Nitrogen fixation by a marine non-heterocystous cyanobacterium requires a heterotrophic bacterial consort.

    abstract::Cultures of the non-heterocystous cyanobacterium, Leptolyngbya nodulosa, could be grown indefinitely in media devoid of combined nitrogen. Acetylene reduction assays showed that these cultures fixed nitrogen in the dark period of a diurnal cycle under micro-oxygenic or anaerobic conditions. Addition of DCMU to culture...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02159.x

    authors: Li Z,Yu J,Kim KR,Brand J

    更新日期:2010-05-01 00:00:00