Abstract:
:Located in the Northern Territory of Australia, Ranger uranium mine is directly adjacent to the UNESCO World Heritage listed Kakadu National Park, with rehabilitation targets needed to ensure the site can be incorporated into the park following the mine's closure in 2026. This study aimed to understand the impact of uranium concentration on microbial communities, in order to identify and describe potential breakpoints in microbial ecosystem services. This is the first study to report in situ deployment of uranium-spiked sediments along a concentration gradient (0-4000 mg U kg-1 ), with the study design maximising the advantages of both field surveys and laboratory manipulative studies. Changes to microbial communities were characterised through the use of amplicon and shotgun metagenomic next-generation sequencing. Significant changes to taxonomic and functional community assembly occurred at a concentration of 1500 mg U kg-1 sediment and above. At uranium concentrations of ≥ 1500 mg U kg-1 , genes associated with methanogenic consortia and processes increased in relative abundance, while numerous significant changes were also seen in the relative abundances of genes involved in nitrogen cycling. Such alterations in carbon and nitrogen cycling pathways suggest that taxonomic and functional changes to microbial communities may result in changes in ecosystem processes and resilience.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Sutcliffe B,Chariton AA,Harford AJ,Hose GC,Greenfield P,Elbourne LDH,Oytam Y,Stephenson S,Midgley DJ,Paulsen ITdoi
10.1111/1462-2920.13839subject
Has Abstractpub_date
2017-08-01 00:00:00pages
3323-3341issue
8eissn
1462-2912issn
1462-2920journal_volume
19pub_type
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