Ribonucleases control distinct traits of Pseudomonas putida lifestyle.

Abstract:

:The role of archetypal ribonucleases (RNases) in the physiology and stress endurance of the soil bacterium and metabolic engineering platform Pseudomonas putida KT2440 has been inspected. To this end, variants of this strain lacking each of the most important RNases were constructed. Each mutant lacked either one exoribonuclease (PNPase, RNase R) or one endoribonuclease (RNase E, RNase III, RNase G). The global physiological and metabolic costs of the absence of each of these enzymes were then analysed in terms of growth, motility and morphology. The effects of different oxidative chemicals that mimic the stresses endured by this microorganism in its natural habitats were studied as well. The results highlighted that each ribonuclease is specifically related with different traits of the environmental lifestyle that distinctively characterizes this microorganism. Interestingly, the physiological responses of P. putida to the absence of each enzyme diverged significantly from those known previously in Escherichia coli. This exposed not only species-specific regulatory functions for otherwise known RNase activities but also expanded the panoply of post-transcriptional adaptation devices that P. putida can make use of for facing hostile environments.

journal_name

Environ Microbiol

authors

Apura P,de Lorenzo V,Arraiano CM,Martínez-García E,Viegas SC

doi

10.1111/1462-2920.15291

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

174-189

issue

1

eissn

1462-2912

issn

1462-2920

journal_volume

23

pub_type

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