Homeostatic control of nitric oxide (NO) at nanomolar concentrations in denitrifying bacteria - modelling and experimental determination of NO reductase kinetics in vivo in Paracoccus denitrificans.

Abstract:

:Homeostatic control of nitric oxide (NO) at nanomolar concentrations appears common among denitrifying bacteria, often ascribed to synchronized expression of nitrite and nitric oxide reductase (Nir and Nor). We questioned whether this is sufficient: using the reported substrate affinities for cytochrome cd1 nitrite reductase (cNor), our model of batch cultures of Paracoccus denitrificans predicted NO concentrations orders of magnitude higher than measured. We rejected a hypothesis that the homeostatic control is due to a negative feedback by NO on the activity of NirS because the inclusion of such feedback resulted in too slow anaerobic growth and N2 production. We proceeded by determining the kinetic parameters for cNor in vivo by a carefully designed experiment, allowing the estimation of NO concentration at the cell surface while anoxic cultures depleted low headspace doses of NO. With the new parameters for cNor kinetics in vivo {v = vmax /[1 + K2 /(NO) + K1  × K2 /(NO)(2) ]; vmax  = 3.56 fmol NO cell(-1)  h(-1) , K1  < 1 nM, and K2  = 34 nM}, the model predicted NO concentrations close to that measured. Thus, enzyme kinetics alone can explain the observed NO homeostasis. Determinations of enzyme kinetic parameters in vivo are not trivial but evidently required to understand and model NO kinetics in denitrifying organisms in soils and aquatic environments.

journal_name

Environ Microbiol

authors

Hassan J,Bergaust LL,Molstad L,de Vries S,Bakken LR

doi

10.1111/1462-2920.13129

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

2964-78

issue

9

eissn

1462-2912

issn

1462-2920

journal_volume

18

pub_type

杂志文章
  • Roseobacter and SAR11 dominate microbial glucose uptake in coastal North Sea waters.

    abstract::Bacterial assemblages in coastal pelagic environments are exposed to pronounced temporal and spatial fluctuations in the availability of monomeric substrates. Little is known about the response of particular bacterial groups to such variability. We studied glucose incorporation at various concentrations (0.1-100 nM) b...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01082.x

    authors: Alonso C,Pernthaler J

    更新日期:2006-11-01 00:00:00

  • A Pseudomonas putida cardiolipin synthesis mutant exhibits increased sensitivity to drugs related to transport functionality.

    abstract::Biological membranes have evolved different mechanisms to modify their composition in response to chemical stimuli in a process called 'homeoviscous adaptation'. Among these mechanisms, modifications in the ratio of saturated/unsaturated fatty acids and in cis/trans fatty acid isomers, cyclopropanation and changes in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01236.x

    authors: Bernal P,Muñoz-Rojas J,Hurtado A,Ramos JL,Segura A

    更新日期:2007-05-01 00:00:00

  • Attachment between heterotrophic bacteria and microalgae influences symbiotic microscale interactions.

    abstract::The surface and surroundings of microalgal cells (phycosphere) are critical interaction zones but have been difficult to functionally interrogate due to methodological limitations. We examined effects of phycosphere-associated bacteria for two biofuel-relevant microalgal species (Phaeodactylum tricornutum and Nannochl...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14357

    authors: Samo TJ,Kimbrel JA,Nilson DJ,Pett-Ridge J,Weber PK,Mayali X

    更新日期:2018-12-01 00:00:00

  • Low-diversity bacterial community in the gut of the fruitfly Drosophila melanogaster.

    abstract::The bacteria in the fruitfly Drosophila melanogaster of different life stages was quantified by 454 pyrosequencing of 16S rRNA gene amplicons. The sequence reads were dominated by 5 operational taxonomic units (OTUs) at ≤ 97% sequence identity that could be assigned to Acetobacter pomorum, A. tropicalis, Lactobacillus...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02511.x

    authors: Wong CN,Ng P,Douglas AE

    更新日期:2011-07-01 00:00:00

  • Methanol metabolism in the acetogenic bacterium Acetobacterium woodii.

    abstract::Methanol derived from plant tissue is ubiquitous in anaerobic sediments and a good substrate for anaerobes growing on C1 compounds such as methanogens and acetogens. In contrast to methanogens little is known about the physiology, biochemistry and bioenergetics of methanol utilization in acetogenic bacteria. To fill t...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14356

    authors: Kremp F,Poehlein A,Daniel R,Müller V

    更新日期:2018-12-01 00:00:00

  • Detection of phosphonoacetate degradation and phnA genes in soil bacteria from distinct geographical origins suggest its possible biogenic origin.

    abstract::Phosphonoacetate is regarded as an antiviral xenobiotic whose mineralization can be catalysed by an enzyme, phosphonoacetate hydrolase, encoded by the phnA gene. To date the enzyme's activity has been detected in only a limited number of bacteria. Its expression has been shown to occur in a manner independent of the p...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00974.x

    authors: Panas P,Ternan NG,Dooley JS,McMullan G

    更新日期:2006-05-01 00:00:00

  • Unravelling lactate-acetate and sugar conversion into butyrate by intestinal Anaerobutyricum and Anaerostipes species by comparative proteogenomics.

    abstract::The d- and l-forms of lactate are important fermentation metabolites produced by intestinal bacteria but are found to negatively affect mucosal barrier function and human health. Both enantiomers of lactate can be converted with acetate into the presumed beneficial butyrate by a phylogenetically related group of anaer...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15269

    authors: Shetty SA,Boeren S,Bui TPN,Smidt H,de Vos WM

    更新日期:2020-11-01 00:00:00

  • Toxin-Antitoxin systems eliminate defective cells and preserve symmetry in Bacillus subtilis biofilms.

    abstract::Toxin-antitoxin modules are gene pairs encoding a toxin and its antitoxin, and are found on the chromosomes of many bacteria, including pathogens. Here, we characterize the specific contribution of the TxpA and YqcG toxins in elimination of defective cells from developing Bacillus subtilis biofilms. On nutrient limita...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13471

    authors: Bloom-Ackermann Z,Steinberg N,Rosenberg G,Oppenheimer-Shaanan Y,Pollack D,Ely S,Storzi N,Levy A,Kolodkin-Gal I

    更新日期:2016-12-01 00:00:00

  • High phenotypic diversity in infecting but not in colonizing Staphylococcus aureus populations.

    abstract::In hostile environments diversity within a bacterial population may be beneficial for the fitness of the microbial community as a whole. Here we analysed the population diversity of Staphylococcus aureus in infecting and colonizing situations. In the study, performed independently in two German centres, the heterogene...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01423.x

    authors: Goerke C,Gressinger M,Endler K,Breitkopf C,Wardecki K,Stern M,Wolz C,Kahl BC

    更新日期:2007-12-01 00:00:00

  • Molecular diversity of methanotrophs in Transbaikal soda lake sediments and identification of potentially active populations by stable isotope probing.

    abstract::Soda lakes are an environment with an unusually high pH and often high salinity. To identify the active methanotrophs in the Soda lake sediments, sediment slurries were incubated with a 10% (v/v) (13)CH(4) headspace and the (13)C-labelled DNA was subsequently extracted from these sediments following CsCl density gradi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00635.x

    authors: Lin JL,Radajewski S,Eshinimaev BT,Trotsenko YA,McDonald IR,Murrell JC

    更新日期:2004-10-01 00:00:00

  • Fungal manganese oxidation in a reduced soil.

    abstract::Manganese chemistry in soils is a function of complex, competing biotic and abiotic reactions. The role of soil-borne fungi in mediating these reactions is poorly understood. The objective of this article is to document direct observation of fungal Mn oxidation in soil under near in situ conditions, and to isolate, de...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00842.x

    authors: Thompson IA,Huber DM,Guest CA,Schulze DG

    更新日期:2005-09-01 00:00:00

  • A new genomic taxonomy system for the Synechococcus collective.

    abstract::Cyanobacteria of the genus Synechococcus are major contributors to global primary productivity and are found in a wide range of aquatic ecosystems. This Synechococcus collective (SC) is metabolically diverse, with some lineages thriving in polar and nutrient-rich locations and others in tropical or riverine waters. Al...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15173

    authors: Salazar VW,Tschoeke DA,Swings J,Cosenza CA,Mattoso M,Thompson CC,Thompson FL

    更新日期:2020-11-01 00:00:00

  • Ammonia-oxidizing communities in a highly aerated full-scale activated sludge bioreactor: betaproteobacterial dynamics and low relative abundance of Crenarchaea.

    abstract::Ammonia-oxidizing bacteria (AOB) have long been considered key to the removal of nitrogen in activated sludge bioreactors. Culture-independent molecular analyses have established that AOB lineages in bioreactors are dynamic, but the underlying operational or environmental factors are unclear. Furthermore, the contribu...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01958.x

    authors: Wells GF,Park HD,Yeung CH,Eggleston B,Francis CA,Criddle CS

    更新日期:2009-09-01 00:00:00

  • Elucidation of the Burkholderia cenocepacia hopanoid biosynthesis pathway uncovers functions for conserved proteins in hopanoid-producing bacteria.

    abstract::Hopanoids are bacterial surrogates of eukaryotic membrane sterols and among earth's most abundant natural products. Their molecular fossils remain in sediments spanning more than a billion years. However, hopanoid metabolism and function are not fully understood. Burkholderia species are environmental opportunistic pa...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12509

    authors: Schmerk CL,Welander PV,Hamad MA,Bain KL,Bernards MA,Summons RE,Valvano MA

    更新日期:2015-03-01 00:00:00

  • Transcriptional profiling of Gram-positive Arthrobacter in the phyllosphere: induction of pollutant degradation genes by natural plant phenolic compounds.

    abstract::Arthrobacter chlorophenolicus A6 is a Gram-positive, 4-chlorophenol-degrading soil bacterium that was recently shown to be an effective colonizer of plant leaf surfaces. The genetic basis for this phyllosphere competency is unknown. In this paper, we describe the genome-wide expression profile of A.chlorophenolicus on...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12375

    authors: Scheublin TR,Deusch S,Moreno-Forero SK,Müller JA,van der Meer JR,Leveau JH

    更新日期:2014-07-01 00:00:00

  • Geomicrobiological exploration and characterization of a novel deep-sea hydrothermal system at the TOTO caldera in the Mariana Volcanic Arc.

    abstract::Novel hydrothermal activities accompanying effluent white smokers and elemental sulfur chimney structures at the north-east lava dome of the TOTO caldera depression in the Mariana Volcanic Arc have been explored and characterized by geochemical and microbiological surveys. White smoker hydrothermal fluids were observe...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00884.x

    authors: Nakagawa T,Takai K,Suzuki Y,Hirayama H,Konno U,Tsunogai U,Horikoshi K

    更新日期:2006-01-01 00:00:00

  • A molecular biological protocol to distinguish potentially human pathogenic Stenotrophomonas maltophilia from plant-associated Stenotrophomonas rhizophila.

    abstract::In recent years, the importance of the Gram-negative bacterium Stenotrophomonas as an opportunistic pathogen as well as in biotechnology has increased. The aim of the present study was to develop new methods for distinguishing between strains closely related to the potentially human pathogenic Stenotrophomonas maltoph...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00928.x

    authors: Ribbeck-Busch K,Roder A,Hasse D,de Boer W,Martínez JL,Hagemann M,Berg G

    更新日期:2005-11-01 00:00:00

  • Bacteriophage influence Campylobacter jejuni types populating broiler chickens.

    abstract::The characteristics that allow one Campylobacter jejuni genotype to succeed over another under the influence of bacteriophage predation have been examined in experimental broiler chickens following the observation that this succession appeared to occur in naturally colonized broiler chicken flocks. Examination of thre...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01351.x

    authors: Scott AE,Timms AR,Connerton PL,El-Shibiny A,Connerton IF

    更新日期:2007-09-01 00:00:00

  • Mas5, a homologue of bacterial DnaJ, is indispensable for the host infection and environmental adaptation of a filamentous fungal insect pathogen.

    abstract::Mas5, a yeast heat shock protein classified to the Hsp40 group, is homologous to bacterial archetype DnaJ but functionally unexplored in filamentous fungi. Here we identify a Mas5 homologue (46.86 kDa) in Beauveria bassiana and show its indispensability for host infection and environmental adaptation of the fungal ins...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13197

    authors: Wang J,Ying SH,Hu Y,Feng MG

    更新日期:2016-03-01 00:00:00

  • Interactive effects of seasonal drought and elevated atmospheric carbon dioxide concentration on prokaryotic rhizosphere communities.

    abstract::Global change models indicate that rainfall patterns are likely to shift towards more extreme events concurrent with increasing atmospheric carbon dioxide concentration ([CO2 ]). Both changes in [CO2 ] and rainfall regime are known to impact above- and belowground communities, but the interactive effects of these glob...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13802

    authors: Drigo B,Nielsen UN,Jeffries TC,Curlevski NJA,Singh BK,Duursma RA,Anderson IC

    更新日期:2017-08-01 00:00:00

  • Pathway of nitrous oxide consumption in isolated Pseudomonas stutzeri strains under anoxic and oxic conditions.

    abstract::The microbial consumption of nitrous oxide (N2O) has gained great interest since it was revealed that this process could mitigate the greenhouse effect of N2O. The consumption of N2O results from its reduction to dinitrogen gas (N2) as part of the denitrification process. However, there is ongoing debate regarding an ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12404

    authors: Desloover J,Roobroeck D,Heylen K,Puig S,Boeckx P,Verstraete W,Boon N

    更新日期:2014-10-01 00:00:00

  • Selection in the host structures the microbiota associated with developing cod larvae (Gadus morhua).

    abstract::Marine fish larvae are immature upon hatching, and share their environment with high numbers of bacteria. The microbial communities associated with developing fish larvae might be structured by other factors than those important in developing terrestrial animals. Here, we analysed the beta (β)-diversity of the microbi...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12888

    authors: Bakke I,Coward E,Andersen T,Vadstein O

    更新日期:2015-10-01 00:00:00

  • Off-axis symbiosis found: Characterization and biogeography of bacterial symbionts of Bathymodiolus mussels from Lost City hydrothermal vents.

    abstract::Organisms at hydrothermal vents inhabit discontinuous chemical 'islands' along mid-ocean ridges, a scenario that may promote genetic divergence among populations. The 2003 discovery of mussels at the Lost City Hydrothermal Field provided a means of evaluating factors that govern the biogeography of symbiotic bacteria ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.01113.x

    authors: DeChaine EG,Bates AE,Shank TM,Cavanaugh CM

    更新日期:2006-11-01 00:00:00

  • AoStuA, an APSES transcription factor, regulates the conidiation, trap formation, stress resistance and pathogenicity of the nematode-trapping fungus Arthrobotrys oligospora.

    abstract::The APSES protein family comprises a conserved class of fungus-specific transcriptional regulators. Some members have been identified in partial ascomycetes. In this study, the APSES protein StuA (AoStuA) of the nematode-trapping fungus Arthrobotrys oligospora was characterized. Compared with the wild-type (WT) strain...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14785

    authors: Xie M,Wang Y,Tang L,Yang L,Zhou D,Li Q,Niu X,Zhang KQ,Yang J

    更新日期:2019-12-01 00:00:00

  • Prevalence and antibiotic resistance pattern of Salmonella serovars in integrated crop-livestock farms and their products sold in local markets.

    abstract::Major concern in the Mixed Crop-Livestock (MCL) farms, in which livestock and vegetables grown closely in the same facility, is cross-contamination of zoonotic bacterial pathogens especially Salmonella. To investigate the distribution of Salmonella serovars in MCL and their products, a total of 1287 pre-harvest sample...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13265

    authors: Peng M,Salaheen S,Almario JA,Tesfaye B,Buchanan R,Biswas D

    更新日期:2016-05-01 00:00:00

  • Insights into networks of functional microbes catalysing methanization of cellulose under mesophilic conditions.

    abstract::DNA-SIP (stable isotope probing) was conducted on anaerobic municipal solid waste samples incubated with (13)C-cellulose, (13)C-glucose and (13)C-acetate under mesophilic conditions. A total of 567 full-length bacterial and 448 1100-bp-length archaeal 16S rRNA gene sequences were analysed. In the clone libraries deriv...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01810.x

    authors: Li T,Mazéas L,Sghir A,Leblon G,Bouchez T

    更新日期:2009-04-01 00:00:00

  • An AbrB-like protein might be involved in the regulation of cylindrospermopsin production by Aphanizomenon ovalisporum.

    abstract::Certain filamentous cyanobacteria, including Aphanizomenon ovalisporum, are potentially toxic owing to the formation of the hepatotoxin cylindrospermopsin. We previously identified a gene cluster in A. ovalisporum likely to be involved in cylindrospermopsin biosynthesis, including amidinotransferase (aoaA) and polyket...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01519.x

    authors: Shalev-Malul G,Lieman-Hurwitz J,Viner-Mozzini Y,Sukenik A,Gaathon A,Lebendiker M,Kaplan A

    更新日期:2008-04-01 00:00:00

  • A dynamic microbial sulfur cycle in a serpentinizing continental ophiolite.

    abstract::Serpentinization is the hydration and oxidation of ultramafic rock, which occurs as oceanic lithosphere is emplaced onto continental margins (ophiolites), and along the seafloor as faulting exposes this mantle-derived material to circulating hydrothermal fluids. This process leads to distinctive fluid chemistries as m...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15006

    authors: Sabuda MC,Brazelton WJ,Putman LI,McCollom TM,Hoehler TM,Kubo MDY,Cardace D,Schrenk MO

    更新日期:2020-06-01 00:00:00

  • The antitoxin MqsA homologue in Pseudomonas fluorescens 2P24 has a rewired regulatory circuit through evolution.

    abstract::The mqsRA operon encodes a toxin-antitoxin pair that was characterized to participate in biofilm and persister cell formation in Escherichia coli. Notably, the antitoxin MqsA possesses a C-terminal DNA-binding domain that recognizes the [5'-AACCT(N)2-4 AGGTT-3'] motif and acts as a transcriptional regulator controllin...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14538

    authors: Wang Y,Zhang SP,Zhang MY,Kempher ML,Guo DD,Han JT,Tao X,Wu Y,Zhang LQ,He YX

    更新日期:2019-05-01 00:00:00

  • Aerotaxis in Desulfovibrio.

    abstract::Aerotaxis of two sulphate-reducing bacteria, the freshwater strain Desulfovibrio desulfuricans CSN (DSM 9104) and the marine strain Desulfovibrio oxyclinae N13 (DSM 11498), was studied using capillary microslides, microscopy and oxygen microsensors. The bacteria formed ring-shaped bands in oxygen diffusion gradients s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.1999.00057.x

    authors: Eschemann A,Kühl M,Cypionka H

    更新日期:1999-12-01 00:00:00