Mas5, a homologue of bacterial DnaJ, is indispensable for the host infection and environmental adaptation of a filamentous fungal insect pathogen.

Abstract:

:Mas5, a yeast heat shock protein classified to the Hsp40 group, is homologous to bacterial archetype DnaJ but functionally unexplored in filamentous fungi. Here we identify a Mas5 homologue (46.86 kDa) in Beauveria bassiana and show its indispensability for host infection and environmental adaptation of the fungal insect pathogen. The deletion of mas5 caused severe defects in aerial conidiation, conidial germination and submerged blastospore production (mimic to host haemocoel). The deletion mutant lost 100% virulence to Galleria mellonella larvae through normal cuticular penetration (topical inoculation) and 50% through cuticle-bypassing infection (intrahaemocoel injection). It formed no blastospore in vivo after inoculation or only a very few after injection. Its extracellular (cuticle degrading) enzymes and virulence-relating Pr1 proteases were 62% and 32% less active respectively. It became more sensitive to high osmolarity, oxidation, cell-wall perturbation, heat shock and UV-B irradiation. These concurred with reduced contents of intracellular mannitol and trehalose, decreased activities of antioxidant enzymes, impaired cell walls and suppressed transcripts of stress-responsive and virulence-relating genes. All the changes were restored by targeted mas5 complementation. All together, Mas5 is indispensable for the in vitro and in vivo life cycle of B. bassiana by targeting many sets of enzymes/proteins at transcriptional and post-transcriptional levels.

journal_name

Environ Microbiol

authors

Wang J,Ying SH,Hu Y,Feng MG

doi

10.1111/1462-2920.13197

subject

Has Abstract

pub_date

2016-03-01 00:00:00

pages

1037-47

issue

3

eissn

1462-2912

issn

1462-2920

journal_volume

18

pub_type

杂志文章
  • Distribution and activity of diazotrophs in the Eastern Equatorial Atlantic.

    abstract::The gene abundance and gene expression of six diazotroph populations from the Eastern Equatorial Atlantic in June 2007 were examined using nifH gene quantitative polymerase chain reaction (q PCR) methods. Of all the diazotrophs, Trichodesmium spp. was the most abundant with the highest number of gene copies in the Gul...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01796.x

    authors: Foster RA,Subramaniam A,Zehr JP

    更新日期:2009-04-01 00:00:00

  • Evidence for selective bacterial community structuring on microplastics.

    abstract::In aquatic ecosystems, microplastics are a relatively new anthropogenic substrate that can readily be colonized by biofilm-forming organisms. To examine the effects of substrate type on microbial community assembly, we exposed ambient Baltic bacterioplankton to plastic substrates commonly found in marine environments ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14120

    authors: Ogonowski M,Motiei A,Ininbergs K,Hell E,Gerdes Z,Udekwu KI,Bacsik Z,Gorokhova E

    更新日期:2018-08-01 00:00:00

  • Metapopulation theory identifies biogeographical patterns among core and satellite marine bacteria scaling from tens to thousands of kilometers.

    abstract::Metapopulation theory developed in terrestrial ecology provides applicable frameworks for interpreting the role of local and regional processes in shaping species distribution patterns. Yet, empirical testing of metapopulation models on microbial communities is essentially lacking. We determined regional bacterioplank...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13650

    authors: Lindh MV,Sjöstedt J,Ekstam B,Casini M,Lundin D,Hugerth LW,Hu YO,Andersson AF,Andersson A,Legrand C,Pinhassi J

    更新日期:2017-03-01 00:00:00

  • Molecular evidence for widespread occurrence of Foraminifera in soils.

    abstract::Environmental SSU rDNA-based surveys are contributing to the dramatic revision of eukaryotic high-level diversity and phylogeny as the number of sequence data increases. This ongoing revolution gives the opportunity to test for the presence of some eukaryotic taxa in environments where they have not been found using c...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02225.x

    authors: Lejzerowicz F,Pawlowski J,Fraissinet-Tachet L,Marmeisse R

    更新日期:2010-09-01 00:00:00

  • Coexistence facilitates interspecific biofilm formation in complex microbial communities.

    abstract::Social interactions in which bacteria respond to one another by modifying their phenotype are central determinants of microbial communities. It is known that interspecific interactions influence the biofilm phenotype of bacteria; a phenotype that is central to the fitness of bacteria. However, the underlying role of f...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13335

    authors: Madsen JS,Røder HL,Russel J,Sørensen H,Burmølle M,Sørensen SJ

    更新日期:2016-09-01 00:00:00

  • Novel, diverse RNA viruses from Mediterranean isolates of the phytopathogenic fungus, Rosellinia necatrix: insights into evolutionary biology of fungal viruses.

    abstract::To reveal mycovirus diversity, we conducted a search of as-yet-unexplored Mediterranean isolates of the phytopathogenic ascomycete Rosellinia necatrix for virus infections. Of seventy-nine, eleven fungal isolates tested RNA virus-positive, with many showing coinfections, indicating a virus incidence of 14%, which is s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14065

    authors: Arjona-Lopez JM,Telengech P,Jamal A,Hisano S,Kondo H,Yelin MD,Arjona-Girona I,Kanematsu S,Lopez-Herrera CJ,Suzuki N

    更新日期:2018-04-01 00:00:00

  • Links between ammonia oxidizer species composition, functional diversity and nitrification kinetics in grassland soils.

    abstract::Molecular approaches have revealed considerable diversity and uncultured novelty in natural prokaryotic populations, but not direct links between the new genotypes detected and ecosystem processes. Here we describe the influence of the structure of communities of ammonia-oxidizing bacteria on nitrogen cycling in micro...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00740.x

    authors: Webster G,Embley TM,Freitag TE,Smith Z,Prosser JI

    更新日期:2005-05-01 00:00:00

  • Comparative analyses of actinobacterial genomic fragments from Lake Kinneret.

    abstract::The high genomic G+C group of Actinobacteria possesses a variety of physiological and metabolic properties, and exhibits diverse lifestyles and ecological distribution. In recent years, Actinobacteria have been found to frequently dominate samples obtained from freshwater samples. Furthermore, phylogenetic analyses ha...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02024.x

    authors: Philosof A,Sabehi G,Béjà O

    更新日期:2009-12-01 00:00:00

  • Multidimensional NMR analysis reveals truncated lignin structures in wood decayed by the brown rot basidiomycete Postia placenta.

    abstract::Lignocellulose biodegradation, an essential step in terrestrial carbon cycling, generally involves removal of the recalcitrant lignin barrier that otherwise prevents infiltration by microbial polysaccharide hydrolases. However, fungi that cause brown rot of wood, a major route for biomass recycling in coniferous fores...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02417.x

    authors: Yelle DJ,Wei D,Ralph J,Hammel KE

    更新日期:2011-04-01 00:00:00

  • Elucidation of the zinc-dependent regulation in Amycolatopsis japonicum enabled the identification of the ethylenediamine-disuccinate ([S,S]-EDDS) genes.

    abstract::The actinomycete Amycolatopsis japonicum produces the complexing agent ethylenediamine-disuccinate ([S,S]-EDDS), which is an isomer of the widely industrially applied ethylenediamine-tetraacetate (EDTA). In contrast to EDTA, [S,S]-EDDS is readily biodegradable and is therefore an alternative with a favourable environm...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13159

    authors: Spohn M,Wohlleben W,Stegmann E

    更新日期:2016-04-01 00:00:00

  • Reduced microbial diversity and high numbers of one single Escherichia coli strain in the intestine of colitic mice.

    abstract::Commensal bacteria play a role in the aetiology of inflammatory bowel diseases (IBD). High intestinal numbers of Escherichia coli in IBD patients suggest a role of this organism in the initiation or progression of chronic gut inflammation. In addition, some E. coli genotypes are more frequently detected in IBD patient...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01883.x

    authors: Wohlgemuth S,Haller D,Blaut M,Loh G

    更新日期:2009-06-01 00:00:00

  • A Pseudomonas putida cardiolipin synthesis mutant exhibits increased sensitivity to drugs related to transport functionality.

    abstract::Biological membranes have evolved different mechanisms to modify their composition in response to chemical stimuli in a process called 'homeoviscous adaptation'. Among these mechanisms, modifications in the ratio of saturated/unsaturated fatty acids and in cis/trans fatty acid isomers, cyclopropanation and changes in ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01236.x

    authors: Bernal P,Muñoz-Rojas J,Hurtado A,Ramos JL,Segura A

    更新日期:2007-05-01 00:00:00

  • Aerobic methane oxidation and methanotroph community composition during seasonal stratification in Mono Lake, California (USA).

    abstract::Patterns of aerobic methane (CH4) oxidation and associated methanotroph community composition were investigated during the development of seasonal stratification in Mono Lake, California (USA). CH4 oxidation rates were measured using a tritiated CH4 radiotracer technique. Fluorescence in situ hybridization (FISH), den...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00786.x

    authors: Carini S,Bano N,LeCleir G,Joye SB

    更新日期:2005-08-01 00:00:00

  • Catalases play differentiated roles in the adaptation of a fungal entomopathogen to environmental stresses.

    abstract::The catalase family of Beauveria bassiana (fungal entomopathogen) consists of catA (spore-specific), catB (secreted), catP (peroxisomal), catC (cytoplasmic) and catD (secreted peroxidase/catalase), which were distinguished in phylogeny and structure and functionally characterized by constructing single-gene disrupted ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2012.02848.x

    authors: Wang ZL,Zhang LB,Ying SH,Feng MG

    更新日期:2013-02-01 00:00:00

  • Exploring bacterial pathogen community dynamics in freshwater beach sediments: A tale of two lakes.

    abstract::Pathogenic bacteria associated with freshwater ecosystems can pose significant health risks particularly where recreational water use is popular. Common water quality assessments involve quantifying indicator Escherichia coli within the water column but neglect to consider physical and geochemical factors and contribu...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14860

    authors: VanMensel D,Chaganti SR,Droppo IG,Weisener CG

    更新日期:2020-02-01 00:00:00

  • Quantitative PCR reveals transient and persistent algal viruses in Lake Ontario, Canada.

    abstract::To determine if different algal viruses (Phycodnaviridae) share common patterns of seasonal abundance, quantitative PCR methods were developed and applied to monitor the abundances of three different viruses in Lake Ontario, Canada over 13 months. Throughout the year, the abundances of two different phycodnavirus polB...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01988.x

    authors: Short SM,Short CM

    更新日期:2009-10-01 00:00:00

  • Cyanobacterial assimilatory nitrate reductase gene diversity in coastal and oligotrophic marine environments.

    abstract::Cyanobacteria are important primary producers in many marine ecosystems and their abundances and growth rates depend on their ability to assimilate various nitrogen sources. To examine the diversity of nitrate-utilizing marine cyanobacteria, we developed PCR primers specific for cyanobacterial assimilatory nitrate red...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01084.x

    authors: Jenkins BD,Zehr JP,Gibson A,Campbell L

    更新日期:2006-12-01 00:00:00

  • Increases in temperature and nutrient availability positively affect methane-cycling microorganisms in Arctic thermokarst lake sediments.

    abstract::Arctic permafrost soils store large amounts of organic matter that is sensitive to temperature increases and subsequent microbial degradation to methane (CH 4 ) and carbon dioxide (CO 2 ). Here, we studied methanogenic and methanotrophic activity and community composition in thermokarst lake sediments from Utqiag˙vik ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14345

    authors: de Jong AEE,In 't Zandt MH,Meisel OH,Jetten MSM,Dean JF,Rasigraf O,Welte CU

    更新日期:2018-12-01 00:00:00

  • Three transcription regulators of the Nss family mediate the adaptive response induced by nitrate, nitric oxide or nitrous oxide in Wolinella succinogenes.

    abstract::Sensing potential nitrogen-containing respiratory substrates such as nitrate, nitrite, hydroxylamine, nitric oxide (NO) or nitrous oxide (N2 O) in the environment and subsequent upregulation of corresponding catabolic enzymes is essential for many microbial cells. The molecular mechanisms of such adaptive responses ar...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13060

    authors: Kern M,Simon J

    更新日期:2016-09-01 00:00:00

  • Prophages of the genus Bifidobacterium as modulating agents of the infant gut microbiota.

    abstract::Phage predation is one of the key forces that shape genetic diversity in bacterial genomes. Phages are also believed to act as modulators of the microbiota composition and, consequently, as agents that drive bacterial speciation in complex bacterial communities. Very little is known about the occurrence and genetic va...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13154

    authors: Lugli GA,Milani C,Turroni F,Tremblay D,Ferrario C,Mancabelli L,Duranti S,Ward DV,Ossiprandi MC,Moineau S,van Sinderen D,Ventura M

    更新日期:2016-07-01 00:00:00

  • Bacterial community shifts in taxa and diversity in response to localized organic loading in the deep sea.

    abstract::The deep sea is a unique and extreme environment characterized by low concentrations of highly recalcitrant carbon. As a consequence, large organic inputs have potential to cause significant perturbation. To assess the impact of organic enrichment on deep sea microbial communities, we investigated bacterial diversity ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02072.x

    authors: Goffredi SK,Orphan VJ

    更新日期:2010-02-01 00:00:00

  • Escherichia coli has multiple enzymes that attack TNT and release nitrogen for growth.

    abstract::There has been a growing interest in the degradation of 2,4,6-trinitrotoluene (TNT) over the last decade, ever since its removal from polluted sites was declared an international environmental priority. Certain aerobic and anaerobic microorganisms are capable of using TNT as an N source, although very few studies have...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01272.x

    authors: González-Pérez MM,van Dillewijn P,Wittich RM,Ramos JL

    更新日期:2007-06-01 00:00:00

  • Microbial succession of nitrate-reducing bacteria in the rhizosphere of Poa alpina across a glacier foreland in the Central Alps.

    abstract::Changes in community structure and activity of the dissimilatory nitrate-reducing community were investigated across a glacier foreland in the Central Alps to gain insight into the successional pattern of this functional group and the driving environmental factors. Bulk soil and rhizosphere soil of Poa alpina was samp...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01051.x

    authors: Deiglmayr K,Philippot L,Tscherko D,Kandeler E

    更新日期:2006-09-01 00:00:00

  • Genome and physiology of the ascomycete filamentous fungus Xeromyces bisporus, the most xerophilic organism isolated to date.

    abstract::Xeromyces bisporus can grow on sugary substrates down to 0.61, an extremely low water activity. Its genome size is approximately 22 Mb. Gene clusters encoding for secondary metabolites were conspicuously absent; secondary metabolites were not detected experimentally. Thus, in its 'dry' but nutrient-rich environment, X...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12596

    authors: Leong SL,Lantz H,Pettersson OV,Frisvad JC,Thrane U,Heipieper HJ,Dijksterhuis J,Grabherr M,Pettersson M,Tellgren-Roth C,Schnürer J

    更新日期:2015-02-01 00:00:00

  • Pyomelanin-producing Pseudomonas aeruginosa selected during chronic infections have a large chromosomal deletion which confers resistance to pyocins.

    abstract::When bacterial lineages make the transition from free-living to permanent association with hosts, they can undergo massive gene losses, for which the selective forces within host tissues are unknown. We identified here melanogenic clinical isolates of Pseudomonas aeruginosa with large chromosomal deletions (66 to 270 ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13336

    authors: Hocquet D,Petitjean M,Rohmer L,Valot B,Kulasekara HD,Bedel E,Bertrand X,Plésiat P,Köhler T,Pantel A,Jacobs MA,Hoffman LR,Miller SI

    更新日期:2016-10-01 00:00:00

  • High phenotypic diversity in infecting but not in colonizing Staphylococcus aureus populations.

    abstract::In hostile environments diversity within a bacterial population may be beneficial for the fitness of the microbial community as a whole. Here we analysed the population diversity of Staphylococcus aureus in infecting and colonizing situations. In the study, performed independently in two German centres, the heterogene...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01423.x

    authors: Goerke C,Gressinger M,Endler K,Breitkopf C,Wardecki K,Stern M,Wolz C,Kahl BC

    更新日期:2007-12-01 00:00:00

  • Dynamics of Vibrio cholerae abundance in Austrian saline lakes, assessed with quantitative solid-phase cytometry.

    abstract::In order to elucidate the main predictors of Vibrio cholerae dynamics and to estimate the risk of Vibrio cholera-related diseases, a recently developed direct detection approach based on fluorescence in situ hybridization and solid-phase cytometry (CARD-FISH/SPC) was applied in comparison to cultivation for water samp...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12861

    authors: Schauer S,Jakwerth S,Bliem R,Baudart J,Lebaron P,Huhulescu S,Kundi M,Herzig A,Farnleitner AH,Sommer R,Kirschner A

    更新日期:2015-11-01 00:00:00

  • Approaches to prokaryotic biodiversity: a population genetics perspective.

    abstract::The study of prokaryotic diversity has blossomed during the last 10-15 years as a result of the introduction of molecular identification, mostly based on direct 16S rRNA gene polymerase chain reaction (PCR) amplification and sequencing from natural samples. A large amount of information exists about the diversity of t...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1462-2920.2002.00354.x

    authors: Rodríguez-Valera F

    更新日期:2002-11-01 00:00:00

  • Basin-scale distribution patterns of picocyanobacterial lineages in the Atlantic Ocean.

    abstract::Marine picocyanobacteria of the genera Prochlorococcus and Synechococcus are major contributors to oceanic primary production. The genera are genetically diverse, comprising several known ecotypes or lineages. However, little is known of the distribution of these lineages over large geographic areas. Here, we analysed...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01246.x

    authors: Zwirglmaier K,Heywood JL,Chamberlain K,Woodward EM,Zubkov MV,Scanlan DJ

    更新日期:2007-05-01 00:00:00

  • What distinguishes cyanobacteria able to revive after desiccation from those that cannot: the genome aspect.

    abstract::Filamentous cyanobacteria are the main founders and primary producers in biological desert soil crusts (BSCs) and are likely equipped to cope with one of the harshest environmental conditions on earth including daily hydration/dehydration cycles, high irradiance and extreme temperatures. Here, we resolved and report o...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13486

    authors: Murik O,Oren N,Shotland Y,Raanan H,Treves H,Kedem I,Keren N,Hagemann M,Pade N,Kaplan A

    更新日期:2017-02-01 00:00:00