Abstract:
:Toxin-antitoxin modules are gene pairs encoding a toxin and its antitoxin, and are found on the chromosomes of many bacteria, including pathogens. Here, we characterize the specific contribution of the TxpA and YqcG toxins in elimination of defective cells from developing Bacillus subtilis biofilms. On nutrient limitation, defective cells accumulated in the biofilm breaking its symmetry. Deletion of the toxins resulted in accumulation of morphologically abnormal cells, and interfered with the proper development of the multicellular community. Dual physiological responses are of significance for TxpA and YqcG activation: nitrogen deprivation enhances the transcription of both TxpA and YqcG toxins, and simultaneously sensitizes the biofilm cells to their activity. Furthermore, we demonstrate that while both toxins when overexpressed affect the morphology of the developing biofilm, the toxin TxpA can act to lyse and dissolve pre-established B. subtilis biofilms.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Bloom-Ackermann Z,Steinberg N,Rosenberg G,Oppenheimer-Shaanan Y,Pollack D,Ely S,Storzi N,Levy A,Kolodkin-Gal Idoi
10.1111/1462-2920.13471subject
Has Abstractpub_date
2016-12-01 00:00:00pages
5032-5047issue
12eissn
1462-2912issn
1462-2920journal_volume
18pub_type
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