Toxin-Antitoxin systems eliminate defective cells and preserve symmetry in Bacillus subtilis biofilms.

Abstract:

:Toxin-antitoxin modules are gene pairs encoding a toxin and its antitoxin, and are found on the chromosomes of many bacteria, including pathogens. Here, we characterize the specific contribution of the TxpA and YqcG toxins in elimination of defective cells from developing Bacillus subtilis biofilms. On nutrient limitation, defective cells accumulated in the biofilm breaking its symmetry. Deletion of the toxins resulted in accumulation of morphologically abnormal cells, and interfered with the proper development of the multicellular community. Dual physiological responses are of significance for TxpA and YqcG activation: nitrogen deprivation enhances the transcription of both TxpA and YqcG toxins, and simultaneously sensitizes the biofilm cells to their activity. Furthermore, we demonstrate that while both toxins when overexpressed affect the morphology of the developing biofilm, the toxin TxpA can act to lyse and dissolve pre-established B. subtilis biofilms.

journal_name

Environ Microbiol

authors

Bloom-Ackermann Z,Steinberg N,Rosenberg G,Oppenheimer-Shaanan Y,Pollack D,Ely S,Storzi N,Levy A,Kolodkin-Gal I

doi

10.1111/1462-2920.13471

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

5032-5047

issue

12

eissn

1462-2912

issn

1462-2920

journal_volume

18

pub_type

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