Free Shiga toxin bacteriophages isolated from sewage showed diversity although the stx genes appeared conserved.

Abstract:

:Phages carrying the stx2 gene were detected in a range of sewage samples using a plaque hybridization-based method. After detection, phages were isolated and propagated with a laboratory strain of Escherichia coli as host for characterization purposes. Although it was not possible to conduct propagation or transduction experiments on most of the phages, 11 reached a sufficiently high titre for studies of host infectivity, electron microscopy and sequencing of the stx2 flanking regions to be performed. These phages showed a wide range of host infectivity and morphology. The genetic structure of the 5' stx flanking region appeared conserved whereas the 3' region differed from that of previously described phages. This is the first description of infectious stx-phages isolated as free particles in the environment, and as such constitutes a new contribution to the study of the ecology of these phages.

journal_name

Environ Microbiol

authors

Muniesa M,Serra-Moreno R,Jofre J

doi

10.1111/j.1462-2920.2004.00604.x

keywords:

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

716-25

issue

7

eissn

1462-2912

issn

1462-2920

pii

EMI604

journal_volume

6

pub_type

杂志文章
  • A wide host-range metagenomic library from a waste water treatment plant yields a novel alcohol/aldehyde dehydrogenase.

    abstract::Using DNA obtained from the metagenome of an anaerobic digestor in a waste water treatment plant, we constructed a gene library cloned in the wide host-range cosmid pLAFR3. One cosmid enabled Rhizobium leguminosarum to grow on ethanol as sole carbon and energy source, this being due to the presence of a gene, termed a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00854.x

    authors: Wexler M,Bond PL,Richardson DJ,Johnston AW

    更新日期:2005-12-01 00:00:00

  • Biofilm susceptibility to metal toxicity.

    abstract::This study compared bacterial biofilm and planktonic cell susceptibility to metal toxicity by evaluating the minimum inhibitory concentration (MIC), the planktonic minimum bactericidal concentration (MBC), and minimum biofilm eradication concentration (MBEC) using the MBEC device. In total, 17 metal cations and oxyani...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00656.x

    authors: Harrison JJ,Ceri H,Stremick CA,Turner RJ

    更新日期:2004-12-01 00:00:00

  • Functional and structural response of a cellulose-degrading methanogenic microbial community to multiple aeration stress at two different temperatures.

    abstract::Two cellulose-fermenting methanogenic enrichment cultures originating from rice soil, one at 15 degrees C with Methanosaeta and the other at 30 degrees C with Methanosarcina as the dominant acetoclastic methanogen, both degraded cellulose anaerobically via propionate, acetate and H2 to CH4. The degradation was a two-s...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00199.x

    authors: Wu XL,Conrad R

    更新日期:2001-06-01 00:00:00

  • Current distribution and potential expansion of the harmful benthic dinoflagellate Ostreopsis cf. siamensis towards the warming waters of the Bay of Biscay, North-East Atlantic.

    abstract::In a future scenario of increasing temperatures in North-Atlantic waters, the risk associated with the expansion of the harmful, benthic dinoflagellate Ostreopsis cf. siamensis has to be evaluated and monitored. Microscopy observations and spatio-temporal surveys of environmental DNA (eDNA) were associated with Lagran...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15406

    authors: Drouet K,Jauzein C,Herviot-Heath D,Hariri S,Laza-Martinez A,Lecadet C,Plus M,Seoane S,Sourisseau M,Lemée R,Siano R

    更新日期:2021-01-26 00:00:00

  • Diversity analyses of Aeschynomene symbionts in Tropical Africa and Central America reveal that nod-independent stem nodulation is not restricted to photosynthetic bradyrhizobia.

    abstract::Tropical aquatic legumes of the genus Aeschynomene are unique in that they can be stem-nodulated by photosynthetic bradyrhizobia. Moreover, a recent study demonstrated that two Aeschynomene indica symbionts lack canonical nod genes, thereby raising questions about the distribution of such atypical symbioses among rhiz...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02090.x

    authors: Miché L,Moulin L,Chaintreuil C,Contreras-Jimenez JL,Munive-Hernández JA,Del Carmen Villegas-Hernandez M,Crozier F,Béna G

    更新日期:2010-08-01 00:00:00

  • Nitrate-dependent iron(II) oxidation in paddy soil.

    abstract::Iron(III) profiles of flooded paddy soil incubated in the greenhouse indicated oxidation of iron(II) in the upper 6 mm soil layer. Measurement of oxygen with a Clark-type microelectrode showed that oxygen was only responsible for the oxidation of iron(II) in the upper 3 mm. In the soil beneath, nitrate could be used a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00163.x

    authors: Ratering S,Schnell S

    更新日期:2001-02-01 00:00:00

  • Non-native acylated homoserine lactones reveal that LuxIR quorum sensing promotes symbiont stability.

    abstract::Quorum sensing, a group behaviour coordinated by a diffusible pheromone signal and a cognate receptor, is typical of bacteria that form symbioses with plants and animals. LuxIR-type N-acyl L-homoserine (AHL) quorum sensing is common in Gram-negative Proteobacteria, and many members of this group have additional quorum...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12322

    authors: Studer SV,Schwartzman JA,Ho JS,Geske GD,Blackwell HE,Ruby EG

    更新日期:2014-08-01 00:00:00

  • A molecular biological protocol to distinguish potentially human pathogenic Stenotrophomonas maltophilia from plant-associated Stenotrophomonas rhizophila.

    abstract::In recent years, the importance of the Gram-negative bacterium Stenotrophomonas as an opportunistic pathogen as well as in biotechnology has increased. The aim of the present study was to develop new methods for distinguishing between strains closely related to the potentially human pathogenic Stenotrophomonas maltoph...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00928.x

    authors: Ribbeck-Busch K,Roder A,Hasse D,de Boer W,Martínez JL,Hagemann M,Berg G

    更新日期:2005-11-01 00:00:00

  • Cyanobacterial assimilatory nitrate reductase gene diversity in coastal and oligotrophic marine environments.

    abstract::Cyanobacteria are important primary producers in many marine ecosystems and their abundances and growth rates depend on their ability to assimilate various nitrogen sources. To examine the diversity of nitrate-utilizing marine cyanobacteria, we developed PCR primers specific for cyanobacterial assimilatory nitrate red...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01084.x

    authors: Jenkins BD,Zehr JP,Gibson A,Campbell L

    更新日期:2006-12-01 00:00:00

  • Characterization of PfiT/PfiA toxin-antitoxin system of Pseudomonas aeruginosa that affects cell elongation and prophage induction.

    abstract::Toxin-antitoxin (TA) systems are small genetic modules usually consisting of two elements-a toxin and an antitoxin. The abundance of TA systems among various bacterial strains may indicate an important evolutionary role. Pseudomonas aeruginosa, which can be found in a variety of niches in nature, is an opportunistic p...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15102

    authors: Zander I,Shmidov E,Roth S,Ben-David Y,Shoval I,Shoshani S,Danielli A,Banin E

    更新日期:2020-12-01 00:00:00

  • The arbuscular mycorrhizal fungus Rhizophagus irregularis uses a reductive iron assimilation pathway for high-affinity iron uptake.

    abstract::Arbuscular mycorrhizal (AM) fungi can improve iron (Fe) acquisition of their host plants. Here, we report a characterization of two components of the high-affinity reductive Fe uptake system of Rhizophagus irregularis, the ferric reductase (RiFRE1) and the high affinity Fe permeases (RiFTR1-2). In the extraradical myc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14121

    authors: Tamayo E,Knight SAB,Valderas A,Dancis A,Ferrol N

    更新日期:2018-05-01 00:00:00

  • Dynamic exometabolome analysis reveals active metabolic pathways in non-replicating mycobacteria.

    abstract::An organism's metabolic activity leaves an extracellular footprint and dynamic changes in this exometabolome inform about nutrient uptake, waste disposal and signalling activities. Using non-targeted mass spectrometry, we report exometabolome dynamics of hypoxia-induced, non-replicating mycobacteria that are thought t...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13056

    authors: Zimmermann M,Kuehne A,Boshoff HI,Barry CE 3rd,Zamboni N,Sauer U

    更新日期:2015-11-01 00:00:00

  • A periplasmic arsenite-binding protein involved in regulating arsenite oxidation.

    abstract::Arsenic (As) is the most common toxic element in the environment, ranking first on the Superfund List of Hazardous Substances. Microbial redox transformations are the principal drivers of As chemical speciation, which in turn dictates As mobility and toxicity. Consequently, in order to manage or remediate environmenta...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02672.x

    authors: Liu G,Liu M,Kim EH,Maaty WS,Bothner B,Lei B,Rensing C,Wang G,McDermott TR

    更新日期:2012-07-01 00:00:00

  • Massively parallel rRNA gene sequencing exacerbates the potential for biased community diversity comparisons due to variable library sizes.

    abstract::Technologies for massively parallel sequencing are revolutionizing microbial ecology and are vastly increasing the scale of ribosomal RNA (rRNA) gene studies. Although pyrosequencing has increased the breadth and depth of possible rRNA gene sampling, one drawback is that the number of reads obtained per sample is diff...

    journal_title:Environmental microbiology

    pub_type: 信件

    doi:10.1111/j.1462-2920.2011.02550.x

    authors: Gihring TM,Green SJ,Schadt CW

    更新日期:2012-02-01 00:00:00

  • Near-zero growth kinetics of Pseudomonas putida deduced from proteomic analysis.

    abstract::Intensive microbial growth typically observed in laboratory rarely occurs in nature. Because of severe nutrient deficiency, natural populations exhibit near-zero growth (NZG). There is a long-standing controversy about sustained NZG, specifically whether there is a minimum growth rate below which cells die or whether ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12584

    authors: Panikov NS,Mandalakis M,Dai S,Mulcahy LR,Fowle W,Garrett WS,Karger BL

    更新日期:2015-01-01 00:00:00

  • Syntrophic associations from hypersaline soda lakes converting organic acids and alcohols to methane at extremely haloalkaline conditions.

    abstract::Until now anaerobic oxidation of VFA at high salt-pH has been demonstrated only at sulfate-reducing conditions. Here, we present results of a microbiological investigation of anaerobic conversion of organic acids and alcohols at methanogenic conditions by syntrophic associations enriched from hypersaline soda lakes in...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13448

    authors: Sorokin DY,Abbas B,Geleijnse M,Kolganova TV,Kleerebezem R,van Loosdrecht MC

    更新日期:2016-09-01 00:00:00

  • Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome.

    abstract::Although common knowledge dictates that the lichen thallus is formed solely by a fungus (mycobiont) that develops a symbiotic relationship with an alga and/or cyanobacterium (photobiont), the non-photoautotrophic bacteria found in lichen microbiomes are increasingly regarded as integral components of lichen thalli. Fo...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2011.02560.x

    authors: Hodkinson BP,Gottel NR,Schadt CW,Lutzoni F

    更新日期:2012-01-01 00:00:00

  • Respiratory quinones in Archaea: phylogenetic distribution and application as biomarkers in the marine environment.

    abstract::The distribution of respiratory quinone electron carriers among cultivated organisms provides clues on both the taxonomy of their producers and the redox processes these are mediating. Our study of the quinone inventories of 25 archaeal species belonging to the phyla Eury-, Cren- and Thaumarchaeota facilitates their u...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13086

    authors: Elling FJ,Becker KW,Könneke M,Schröder JM,Kellermann MY,Thomm M,Hinrichs KU

    更新日期:2016-02-01 00:00:00

  • Novel acid resistance genes from the metagenome of the Tinto River, an extremely acidic environment.

    abstract::Microorganisms that thrive in acidic environments are endowed with specialized molecular mechanisms to survive under this extremely harsh condition. In this work, we performed functional screening of six metagenomic libraries from planktonic and rhizosphere microbial communities of the Tinto River, an extremely acidic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12021

    authors: Guazzaroni ME,Morgante V,Mirete S,González-Pastor JE

    更新日期:2013-04-01 00:00:00

  • A three-component signalling system fine-tunes expression kinetics of HPPK responsible for folate synthesis by positive feedback loop during stress response of Xanthomonas campestris.

    abstract::During adaptation to environments, bacteria employ two-component signal transduction systems, which contain histidine kinases and response regulators, to sense and respond to exogenous and cellular stimuli in an accurate spatio-temporal manner. Although the protein phosphorylation process between histidine kinase and ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12293

    authors: Wang FF,Deng CY,Cai Z,Wang T,Wang L,Wang XZ,Chen XY,Fang RX,Qian W

    更新日期:2014-07-01 00:00:00

  • Differences in the species composition of arbuscular mycorrhizal fungi in spore, root and soil communities in a grassland ecosystem.

    abstract::Most studies on the species composition of arbuscular mycorrhizal fungi (AMF) have solely analysed mycorrhizal roots or AM spores collected from soil samples. However, the spore production rate and proportions of AMF mycelium in roots and soils have all been shown to vary substantially in a taxon-specific manner. Ther...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01309.x

    authors: Hempel S,Renker C,Buscot F

    更新日期:2007-08-01 00:00:00

  • The local environment determines the assembly of root endophytic fungi at a continental scale.

    abstract::Root endophytic fungi are found in a great variety of plants and ecosystems, but the ecological drivers of their biogeographic distribution are poorly understood. Here, we investigate the occurrence of root endophytes in the non-mycorrhizal plant genus Microthlaspi, and the effect of environmental factors and geograph...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13112

    authors: Glynou K,Ali T,Buch AK,Haghi Kia S,Ploch S,Xia X,Çelik A,Thines M,Maciá-Vicente JG

    更新日期:2016-09-01 00:00:00

  • Organohalide respiration in pristine environments: implications for the natural halogen cycle.

    abstract::Halogenated organic compounds, also termed organohalogens, were initially considered to be of almost exclusively anthropogenic origin. However, over 5000 naturally synthesized organohalogens are known today. This has also fuelled the hypothesis that the natural and ancient origin of organohalogens could have primed de...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/1462-2920.14016

    authors: Atashgahi S,Häggblom MM,Smidt H

    更新日期:2018-03-01 00:00:00

  • Chromomycin, an antibiotic produced by Streptomyces flaviscleroticus might play a role in the resistance to oxidative stress and is essential for viability in stationary phase.

    abstract::The well-known role of antibiotics in killing sensitive organisms has been challenged by the effects they exert at subinhibitory concentrations. Unfortunately, there are very few published reports on the advantages these molecules may confer to their producers. This study describes the construction of a genetically ve...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14515

    authors: Prajapati D,Kumari N,Dave K,Chatupale V,Pohnerkar J

    更新日期:2019-02-01 00:00:00

  • Distribution of specific tetracycline and erythromycin resistance genes in environmental samples assessed by macroarray detection.

    abstract::A macroarray system was developed to screen environmental samples for the presence of specific tetracycline (Tc(R)) and erythromycin (erm(R)) resistance genes. The macroarray was loaded with polymerase chain reaction (PCR) amplicons of 23 Tc(R) genes and 10 erm(R) genes. Total bacterial genomic DNA was extracted from ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2006.01190.x

    authors: Patterson AJ,Colangeli R,Spigaglia P,Scott KP

    更新日期:2007-03-01 00:00:00

  • Predominance of deterministic microbial community dynamics in salterns exposed to different light intensities.

    abstract::While the dynamics of microbial community assembly driven by environmental perturbations have been extensively studied, our understanding is far from complete, particularly for light-induced perturbations. Extremely halophilic communities thriving in coastal solar salterns are mainly influenced by two environmental fa...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14790

    authors: Viver T,Orellana LH,Díaz S,Urdiain M,Ramos-Barbero MD,González-Pastor JE,Oren A,Hatt JK,Amann R,Antón J,Konstantinidis KT,Rosselló-Móra R

    更新日期:2019-11-01 00:00:00

  • Distribution of S-layers on the surface of Bacillus cereus strains: phylogenetic origin and ecological pressure.

    abstract::Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis have been described as members of the Bacillus cereus group but are, in fact, one species. B. anthracis is a mammal pathogen, B. thuringiensis an entomopathogen and B. cereus a ubiquitous soil bacterium and an occasional human pathogen. In two clinical iso...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2001.00220.x

    authors: Mignot T,Denis B,Couture-Tosi E,Kolstø AB,Mock M,Fouet A

    更新日期:2001-08-01 00:00:00

  • Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp.

    abstract::In this study, the putative role of phenazines and rhamnolipid-biosurfactants, antagonistic metabolites produced by Pseudomonas aeruginosa PNA1, was tested in the biological control of Pythium splendens on bean (Phaseolus vulgaris L) and Pythium myriotylum on cocoyam (Xanthosoma sagittifolium L Schott). A rhamnolipid-...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01501.x

    authors: Perneel M,D'hondt L,De Maeyer K,Adiobo A,Rabaey K,Höfte M

    更新日期:2008-03-01 00:00:00

  • Detection of abundant sulphate-reducing bacteria in marine oxic sediment layers by a combined cultivation and molecular approach.

    abstract::The depth distribution and diversity of sulphate-reducing bacteria (SRB) was analysed in the upper intertidal zone of a sandy marine sediment of the Dutch island Schiermonnikoog. The upper centimetre of the sediment included the oxic-anoxic interface and was cut into five slices. With each slice, most probable number ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2000.00123.x

    authors: Wieringa EB,Overmann J,Cypionka H

    更新日期:2000-08-01 00:00:00

  • Bacterial, fungal, and plant communities exhibit no biomass or compositional response to two years of simulated nitrogen deposition in a semiarid grassland.

    abstract::Nitrogen (N) deposition affects myriad aspects of terrestrial ecosystem structure and function, and microbial communities may be particularly sensitive to anthropogenic N inputs. However, our understanding of N deposition effects on microbial communities is far from complete, especially for drylands where data are com...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13678

    authors: McHugh TA,Morrissey EM,Mueller RC,Gallegos-Graves V,Kuske CR,Reed SC

    更新日期:2017-04-01 00:00:00