Abstract:
:Phages carrying the stx2 gene were detected in a range of sewage samples using a plaque hybridization-based method. After detection, phages were isolated and propagated with a laboratory strain of Escherichia coli as host for characterization purposes. Although it was not possible to conduct propagation or transduction experiments on most of the phages, 11 reached a sufficiently high titre for studies of host infectivity, electron microscopy and sequencing of the stx2 flanking regions to be performed. These phages showed a wide range of host infectivity and morphology. The genetic structure of the 5' stx flanking region appeared conserved whereas the 3' region differed from that of previously described phages. This is the first description of infectious stx-phages isolated as free particles in the environment, and as such constitutes a new contribution to the study of the ecology of these phages.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Muniesa M,Serra-Moreno R,Jofre Jdoi
10.1111/j.1462-2920.2004.00604.xkeywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
716-25issue
7eissn
1462-2912issn
1462-2920pii
EMI604journal_volume
6pub_type
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