Abstract:
:This study compared bacterial biofilm and planktonic cell susceptibility to metal toxicity by evaluating the minimum inhibitory concentration (MIC), the planktonic minimum bactericidal concentration (MBC), and minimum biofilm eradication concentration (MBEC) using the MBEC device. In total, 17 metal cations and oxyanions, chosen to represent groups VIB to VIA of the periodic table, were each tested on biofilm and planktonic cultures of Escherichia coli JM109, Staphylococcus aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853. In contrast to control antibiotic assays, where biofilm cultures were 2 to 64 times less susceptible to killing than logarithmically growing planktonic bacteria, metal compounds killed planktonic and biofilm cultures at the same concentration in the vast majority of combinations. Our data indicate that, under the conditions reported, growth in a biofilm does not provide resistance to bacteria against killing by metal cations or oxyanions.
journal_name
Environ Microbioljournal_title
Environmental microbiologyauthors
Harrison JJ,Ceri H,Stremick CA,Turner RJdoi
10.1111/j.1462-2920.2004.00656.xkeywords:
subject
Has Abstractpub_date
2004-12-01 00:00:00pages
1220-7issue
12eissn
1462-2912issn
1462-2920pii
EMI656journal_volume
6pub_type
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