Distribution of S-layers on the surface of Bacillus cereus strains: phylogenetic origin and ecological pressure.

Abstract:

:Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis have been described as members of the Bacillus cereus group but are, in fact, one species. B. anthracis is a mammal pathogen, B. thuringiensis an entomopathogen and B. cereus a ubiquitous soil bacterium and an occasional human pathogen. In two clinical isolates of B. cereus, in some B. thuringiensis strains and in B. anthracis, an S-layer has been described. We investigated how the S-layer is distributed in B. cereus, and whether phylogeny or ecology could explain its presence on the surface of some but not all strains. We first developed a simple biochemical assay to test for the presence of the S-layer. We then used the assay with 51 strains of known genetic relationship: 26 genetically diverse B. cereus and 25 non-B. anthracis of the B. anthracis cluster. When present, the genetic organization of the S-layer locus was analysed further. It was identical in B. cereus and B. anthracis. Nineteen strains harboured an S-layer, 16 of which belonged to the B. anthracis cluster. All 19 were B. cereus clinical isolates or B. thuringiensis, except for one soil and one dairy strain. These findings suggest a common phylogenetic origin for the S-layer at the surface of B. cereus strains and, presumably, ecological pressure on its maintenance.

journal_name

Environ Microbiol

authors

Mignot T,Denis B,Couture-Tosi E,Kolstø AB,Mock M,Fouet A

doi

10.1046/j.1462-2920.2001.00220.x

keywords:

subject

Has Abstract

pub_date

2001-08-01 00:00:00

pages

493-501

issue

8

eissn

1462-2912

issn

1462-2920

pii

220

journal_volume

3

pub_type

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