Detection of abundant sulphate-reducing bacteria in marine oxic sediment layers by a combined cultivation and molecular approach.

Abstract:

:The depth distribution and diversity of sulphate-reducing bacteria (SRB) was analysed in the upper intertidal zone of a sandy marine sediment of the Dutch island Schiermonnikoog. The upper centimetre of the sediment included the oxic-anoxic interface and was cut into five slices. With each slice, most probable number (MPN) dilution series were set up in microtitre plates using five different substrates. In the deeper sediment layers, up to 1 x 10(8) cm(-3) lactate-utilizing SRB were counted, corresponding to 23% of the total bacterial count. From the highest positive dilutions of the MPN series, 27 strains of SRB were isolated in pure culture. Sequencing of a 580 bp fragment of the 16S rDNA revealed that 21 isolates had identical sequences, also identical with that of the previously described species Desulfomicrobium apsheronum. However, the diversity of the isolates was higher with respect to their physiological properties: a total of 11 different phenotypes could be distinguished. Genomic fingerprinting by enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR) revealed an even higher diversity of 22 different genotypes. A culture-independent analysis by PCR and denaturing-gradient gel electrophoresis (DGGE) revealed that the partial 16S rDNA sequence of the isolated D. apsheronum strains constituted a significant fraction of the Desulfovibrionaceae. The high subspecies diversity suggests that this abundant aggregate-forming species may have evolved adaptations to different ecological niches in the oxic sediment layers.

journal_name

Environ Microbiol

authors

Wieringa EB,Overmann J,Cypionka H

doi

10.1046/j.1462-2920.2000.00123.x

keywords:

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

417-27

issue

4

eissn

1462-2912

issn

1462-2920

journal_volume

2

pub_type

杂志文章
  • The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina.

    abstract::Reactive oxygen species (ROS) play an important role during host-pathogen interactions and are often an indication of induced host defence responses. In this study, we demonstrate for the first time that Puccinia triticina (Pt) generates ROS, including superoxide, H2 O2 and hydroxyl radicals, during wheat infection. T...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.15063

    authors: Wang X,Che MZ,Khalil HB,McCallum BD,Bakkeren G,Rampitsch C,Saville BJ

    更新日期:2020-07-01 00:00:00

  • Mixing of water masses caused by a drifting iceberg affects bacterial activity, community composition and substrate utilization capability in the Southern Ocean.

    abstract::The number of icebergs produced from ice-shelf disintegration has increased over the past decade in Antarctica. These drifting icebergs mix the water column, influence stratification and nutrient condition, and can affect local productivity and food web composition. Data on whether icebergs affect bacterioplankton fun...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13769

    authors: Dinasquet J,Richert I,Logares R,Yager P,Bertilsson S,Riemann L

    更新日期:2017-06-01 00:00:00

  • Autophagy-related gene BbATG11 is indispensable for pexophagy and mitophagy, and contributes to stress response, conidiation and virulence in the insect mycopathogen Beauveria bassiana.

    abstract::Autophagy is a conserved degradation system in eukaryotic cells that includes non-selective and selective processes. Selective autophagy functions as a selective degradation mechanism for specific substrates in which autophagy-related protein 11 (ATG11) acts as an essential scaffold protein. In B. bassiana, there is a...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14329

    authors: Ding JL,Peng YJ,Chu XL,Feng MG,Ying SH

    更新日期:2018-09-01 00:00:00

  • Hypolithic microbial communities: between a rock and a hard place.

    abstract::Drylands are the largest terrestrial biome on Earth and a ubiquitous feature is desert pavement terrain, comprising rocks embedded in the mineral soil surface. Quartz and other translucent rocks are common and microbial communities termed hypoliths develop as biofilms on their ventral surfaces. In extreme deserts thes...

    journal_title:Environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1462-2920.2012.02821.x

    authors: Chan Y,Lacap DC,Lau MC,Ha KY,Warren-Rhodes KA,Cockell CS,Cowan DA,McKay CP,Pointing SB

    更新日期:2012-09-01 00:00:00

  • Methanol metabolism in the acetogenic bacterium Acetobacterium woodii.

    abstract::Methanol derived from plant tissue is ubiquitous in anaerobic sediments and a good substrate for anaerobes growing on C1 compounds such as methanogens and acetogens. In contrast to methanogens little is known about the physiology, biochemistry and bioenergetics of methanol utilization in acetogenic bacteria. To fill t...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14356

    authors: Kremp F,Poehlein A,Daniel R,Müller V

    更新日期:2018-12-01 00:00:00

  • Effect of environmental variables on eukaryotic microbial community structure of land-fast Arctic sea ice.

    abstract::Sea ice microbial community structure affects carbon and nutrient cycling in polar seas, but its susceptibility to changing environmental conditions is not well understood. We studied the eukaryotic microbial community in sea ice cores recovered near Point Barrow, AK in May 2006 by documenting the composition of the c...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02126.x

    authors: Eddie B,Juhl A,Krembs C,Baysinger C,Neuer S

    更新日期:2010-03-01 00:00:00

  • The arbuscular mycorrhizal fungus Rhizophagus irregularis uses a reductive iron assimilation pathway for high-affinity iron uptake.

    abstract::Arbuscular mycorrhizal (AM) fungi can improve iron (Fe) acquisition of their host plants. Here, we report a characterization of two components of the high-affinity reductive Fe uptake system of Rhizophagus irregularis, the ferric reductase (RiFRE1) and the high affinity Fe permeases (RiFTR1-2). In the extraradical myc...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14121

    authors: Tamayo E,Knight SAB,Valderas A,Dancis A,Ferrol N

    更新日期:2018-05-01 00:00:00

  • Microbial diversity in ikaite tufa columns: an alkaline, cold ecological niche in Greenland.

    abstract::Ikaite tufa columns from the Ikka Fjord in south-western Greenland constitute a natural, stable environment at low temperature and with a pH ranging from neutral at the exterior to very alkaline (pH 10.4) at the interior of the column. Phylogenetic analysis of culturable organisms revealed ten different isolates repre...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00327.x

    authors: Stougaard P,Jørgensen F,Johnsen MG,Hansen OC

    更新日期:2002-08-01 00:00:00

  • Effects of environmental conditions on the nitrifying population dynamics in a pilot wastewater treatment plant.

    abstract::The effect of environmental conditions, especially ammonium concentration, on community composition and nitrification activity of nitrifying bacterial biofilms in a pilot wastewater treatment plant was examined. A decreasing ammonium gradient was created when four aerated tanks with suspended carrier material were ser...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2007.01336.x

    authors: Lydmark P,Almstrand R,Samuelsson K,Mattsson A,Sörensson F,Lindgren PE,Hermansson M

    更新日期:2007-09-01 00:00:00

  • Control and host-dependent activation of insect toxin expression in a root-associated biocontrol pseudomonad.

    abstract::Pseudomonas fluorescens CHA0 is a root-associated biocontrol agent that suppresses soil-borne fungal diseases of crops. Remarkably, the pseudomonad is also endowed with systemic and oral activity against pest insects which depends on the production of the insecticidal Fit toxin. The toxin gene (fitD) is part of a viru...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12050

    authors: Péchy-Tarr M,Borel N,Kupferschmied P,Turner V,Binggeli O,Radovanovic D,Maurhofer M,Keel C

    更新日期:2013-03-01 00:00:00

  • Identification and characterization of naturally occurring DSF-family quorum sensing signal turnover system in the phytopathogen Xanthomonas.

    abstract::Molecules of the diffusible signal factor (DSF)-family are a class of quorum sensing (QS) signals used by the phytopathogens Xanthomonas. Studies during the last decade have outlined how Xanthomonas cells enter the QS phase. However, information on the mechanism underlying its exit from the QS phase is limited. RpfB h...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12999

    authors: Zhou L,Wang XY,Sun S,Yang LC,Jiang BL,He YW

    更新日期:2015-11-01 00:00:00

  • Effects of diurnal temperature variation on microbial community and petroleum hydrocarbon biodegradation in contaminated soils from a sub-Arctic site.

    abstract::Contaminated soils are subject to diurnal and seasonal temperature variations during on-site ex-situ bioremediation processes. We assessed how diurnal temperature variations similar to that in summer at the site from which petroleum hydrocarbon-contaminated soil was collected affect the soil microbial community and th...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12846

    authors: Akbari A,Ghoshal S

    更新日期:2015-12-01 00:00:00

  • AoStuA, an APSES transcription factor, regulates the conidiation, trap formation, stress resistance and pathogenicity of the nematode-trapping fungus Arthrobotrys oligospora.

    abstract::The APSES protein family comprises a conserved class of fungus-specific transcriptional regulators. Some members have been identified in partial ascomycetes. In this study, the APSES protein StuA (AoStuA) of the nematode-trapping fungus Arthrobotrys oligospora was characterized. Compared with the wild-type (WT) strain...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14785

    authors: Xie M,Wang Y,Tang L,Yang L,Zhou D,Li Q,Niu X,Zhang KQ,Yang J

    更新日期:2019-12-01 00:00:00

  • Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing.

    abstract::It is well recognized that a dynamic biofilm develops upon plant biomass in the herbivore gastrointestinal tract, but this component of the microbiome has not previously been specifically sampled, or directly compared with the biodiversity present in the planktonic fraction of digesta. In this study, the digesta colle...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00721.x

    authors: Larue R,Yu Z,Parisi VA,Egan AR,Morrison M

    更新日期:2005-04-01 00:00:00

  • The genome and structural proteome of an ocean siphovirus: a new window into the cyanobacterial 'mobilome'.

    abstract::Prochlorococcus, an abundant phototroph in the oceans, are infected by members of three families of viruses: myo-, podo- and siphoviruses. Genomes of myo- and podoviruses isolated on Prochlorococcus contain DNA replication machinery and virion structural genes homologous to those from coliphages T4 and T7 respectively...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02081.x

    authors: Sullivan MB,Krastins B,Hughes JL,Kelly L,Chase M,Sarracino D,Chisholm SW

    更新日期:2009-11-01 00:00:00

  • Elucidation of the zinc-dependent regulation in Amycolatopsis japonicum enabled the identification of the ethylenediamine-disuccinate ([S,S]-EDDS) genes.

    abstract::The actinomycete Amycolatopsis japonicum produces the complexing agent ethylenediamine-disuccinate ([S,S]-EDDS), which is an isomer of the widely industrially applied ethylenediamine-tetraacetate (EDTA). In contrast to EDTA, [S,S]-EDDS is readily biodegradable and is therefore an alternative with a favourable environm...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13159

    authors: Spohn M,Wohlleben W,Stegmann E

    更新日期:2016-04-01 00:00:00

  • Physiological and cell morphology adaptation of Bacillus subtilis at near-zero specific growth rates: a transcriptome analysis.

    abstract::Nutrient scarcity is a common condition in nature, but the resulting extremely low growth rates (below 0.025 h(-1) ) are an unexplored research area in Bacillus subtilis. To understand microbial life in natural environments, studying the adaptation of B. subtilis to near-zero growth conditions is relevant. To this end...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12676

    authors: Overkamp W,Ercan O,Herber M,van Maris AJ,Kleerebezem M,Kuipers OP

    更新日期:2015-02-01 00:00:00

  • Structural and functional patterns of bacterial communities in response to protist predation along an experimental productivity gradient.

    abstract::Substrate supply and protist grazing are two of the most important forces that determine the composition and properties of bacterial assemblages. General ecological theory predicts that the relative importance of these factors is changing with the environmental productivity. In the present study, the interplay between...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2008.01713.x

    authors: Corno G,Jürgens K

    更新日期:2008-10-01 00:00:00

  • Quantitative PCR reveals transient and persistent algal viruses in Lake Ontario, Canada.

    abstract::To determine if different algal viruses (Phycodnaviridae) share common patterns of seasonal abundance, quantitative PCR methods were developed and applied to monitor the abundances of three different viruses in Lake Ontario, Canada over 13 months. Throughout the year, the abundances of two different phycodnavirus polB...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.01988.x

    authors: Short SM,Short CM

    更新日期:2009-10-01 00:00:00

  • Chromomycin, an antibiotic produced by Streptomyces flaviscleroticus might play a role in the resistance to oxidative stress and is essential for viability in stationary phase.

    abstract::The well-known role of antibiotics in killing sensitive organisms has been challenged by the effects they exert at subinhibitory concentrations. Unfortunately, there are very few published reports on the advantages these molecules may confer to their producers. This study describes the construction of a genetically ve...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14515

    authors: Prajapati D,Kumari N,Dave K,Chatupale V,Pohnerkar J

    更新日期:2019-02-01 00:00:00

  • Soil bacterial diversity in the Arctic is not fundamentally different from that found in other biomes.

    abstract::The severe environmental stresses of the Arctic may have promoted unique soil bacterial communities compared with those found in lower latitude environments. Here, we present a comprehensive analysis of the biogeography of soil bacterial communities in the Arctic using a high resolution bar-coded pyrosequencing techni...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2010.02277.x

    authors: Chu H,Fierer N,Lauber CL,Caporaso JG,Knight R,Grogan P

    更新日期:2010-11-01 00:00:00

  • Bacterial diversity in the rhizosphere of Proteaceae species.

    abstract::The Cape Floral Kingdom is an area of unique plant biodiversity in South Africa with exceptional concentrations of rare and endemic species and experiencing drastic habitat loss. Here we present the first molecular study of the microbial diversity associated with the rhizosphere soil of endemic plants of the Proteacea...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2005.00929.x

    authors: Stafford WH,Baker GC,Brown SA,Burton SG,Cowan DA

    更新日期:2005-11-01 00:00:00

  • Microbial community of a saline mud volcano at San Biagio-Belpasso, Mt. Etna (Italy).

    abstract::In San Biagio of Belpasso, approximately 20 km south of Mt. Etna, in the area of contact between volcanic and sedimentary formations, a number of small (3- 60 cm in diameter) active mud eruptions discharge CO2-rich gases, mud and NaCl brines. They can be described as mini-volcanoes owing to their typical conic shapes ...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1462-2920.2002.00293.x

    authors: Yakimov MM,Giuliano L,Crisafi E,Chernikova TN,Timmis KN,Golyshin PN

    更新日期:2002-05-01 00:00:00

  • The tail-associated depolymerase of Erwinia amylovora phage L1 mediates host cell adsorption and enzymatic capsule removal, which can enhance infection by other phage.

    abstract::The depolymerase enzyme (DpoL1) encoded by the T7-like phage L1 efficiently degrades amylovoran, an important virulence factor and major component of the extracellular polysaccharide (EPS) of its host, the plant pathogen Erwinia amylovora. Mass spectrometry analysis of hydrolysed EPS revealed that DpoL1 cleaves the ga...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.12212

    authors: Born Y,Fieseler L,Klumpp J,Eugster MR,Zurfluh K,Duffy B,Loessner MJ

    更新日期:2014-07-01 00:00:00

  • Identification of inner membrane translocase components of TolC-mediated secretion in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract::Cyanobacteria were the first organisms ever to perform oxygenic photosynthesis and still significantly contribute to primary production on a global scale. To assure the proper functioning of their primary metabolism and cell homeostasis, cyanobacteria must rely on efficient transport systems to cross their multilayere...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14095

    authors: Gonçalves CF,Pacheco CC,Tamagnini P,Oliveira P

    更新日期:2018-07-01 00:00:00

  • Biofilm susceptibility to metal toxicity.

    abstract::This study compared bacterial biofilm and planktonic cell susceptibility to metal toxicity by evaluating the minimum inhibitory concentration (MIC), the planktonic minimum bactericidal concentration (MBC), and minimum biofilm eradication concentration (MBEC) using the MBEC device. In total, 17 metal cations and oxyani...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2004.00656.x

    authors: Harrison JJ,Ceri H,Stremick CA,Turner RJ

    更新日期:2004-12-01 00:00:00

  • Exploring bacterial pathogen community dynamics in freshwater beach sediments: A tale of two lakes.

    abstract::Pathogenic bacteria associated with freshwater ecosystems can pose significant health risks particularly where recreational water use is popular. Common water quality assessments involve quantifying indicator Escherichia coli within the water column but neglect to consider physical and geochemical factors and contribu...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14860

    authors: VanMensel D,Chaganti SR,Droppo IG,Weisener CG

    更新日期:2020-02-01 00:00:00

  • Heterotrophic bacteria from an extremely phosphate-poor lake have conditionally reduced phosphorus demand and utilize diverse sources of phosphorus.

    abstract::Heterotrophic Proteobacteria and Actinobacteria were isolated from Lake Matano, Indonesia, a stratified, ferruginous (iron-rich), ultra-oligotrophic lake with phosphate concentrations below 50 nM. Here, we describe the growth of eight strains of heterotrophic bacteria on a variety of soluble and insoluble sources of p...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.13063

    authors: Yao M,Elling FJ,Jones C,Nomosatryo S,Long CP,Crowe SA,Antoniewicz MR,Hinrichs KU,Maresca JA

    更新日期:2016-02-01 00:00:00

  • Non-canonical localization of RubisCO under high-light conditions in the toxic cyanobacterium Microcystis aeruginosa PCC7806.

    abstract::The frequent production of the hepatotoxin microcystin (MC) and its impact on the lifestyle of bloom-forming cyanobacteria are poorly understood. Here, we report that MC interferes with the assembly and the subcellular localization of RubisCO, in Microcystis aeruginosa PCC7806. Immunofluorescence, electron microscopic...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/1462-2920.14837

    authors: Barchewitz T,Guljamow A,Meissner S,Timm S,Henneberg M,Baumann O,Hagemann M,Dittmann E

    更新日期:2019-12-01 00:00:00

  • Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene.

    abstract::Butyrate-producing bacteria play an important role in the human colon, supplying energy to the gut epithelium and regulating host cell responses. In order to explore the diversity and culturability of this functional group, we designed degenerate primers to amplify butyryl-CoA:acetate CoA-transferase sequences from fa...

    journal_title:Environmental microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1462-2920.2009.02066.x

    authors: Louis P,Young P,Holtrop G,Flint HJ

    更新日期:2010-02-01 00:00:00